Journal: Cell reports

Article Title: Targeted gene transfer into developmentally defined cell populations of the primate brain

doi: 10.1016/j.celrep.2025.116756

Figure Lengend Snippet: (A) Representative coronal sections in a 3-day-old marmoset show the widespread transduction pattern following a PC87 injection of AAV9-CAG-GFP. The side view of the extracted central nervous system highlights broad GFP labeling. Across all regions, the most abundant labeling is in the lower layers. (B–E) Detailed view of the insets shown in (A), highlighting the labeling pattern in the temporal, prefrontal, frontal, and parietal regions. Most labeled cells in the cortex are neurons, based on morphology and co-expression of NeuN (B, white arrowheads in yellow inset). Labeling in more superficial layers is almost entirely restricted to apical dendrites from lower-layer neurons (B, pink inset). The density of transduced neurons varied across cortical depths (dashed boxes in B–E). GFP+/NeuN+ cells were located in similar layers across regions, but the proportion of transduced neurons varied with depth, with up to 80% of cells being transduced at certain depths. (F–I) Cell labeling was observed in additional structures, such as the olfactory bulbs (OBs; A-1), striatum (Str; A-3, A-4, and F), hippocampus and choroid plexus (Hipp and CP, respectively; A-5 and G), and cerebellum (A-7 and I). Strong fiber labeling was observed in the thalamus (Th; A-5 and H) and white matter paths, such as corticofugal pathways within the internal capsule (F). Scale bars: (A) 1 mm, (B–H) 500 μm, and (I) and insets in (G) and (H), 250 μm. FIVI volume: 60 μL.

Article Snippet: AAV9-CAG-GFP , Addgene , 37825-AAV9.

Techniques: Transduction, Injection, Labeling, Expressing, Olfactory