Journal: Poultry Science

Article Title: ERAD inhibitor Eeyarestatin I (EerI) suppresses duck plague virus by restoring STING-mediated antiviral immunity

doi: 10.1016/j.psj.2026.106808

Figure Lengend Snippet: EerI attenuates DPV-induced STING degradation and innate immune suppression. (A) The DEF cells transfected with STING-Myc were infected with DPV-CHv-GFP at an MOI of 1, and treated with EerI (10 μM). At 24 h after infection, CHX (10 μM) was added, and the whole cell lysates were collected at specified time points after the addition of CHX. Protein expression levels were analyzed by Western blot. (B) The relative expression of STING in (A). (C) DEF cells transfected with STING-Myc were infected with DPV-CHv-GFP at an MOI of 1. At 24 h post-infection, MG132 (10 μM) or BAF A1 (200 nM) was added. Whole-cell lysates were collected 12 h after inhibitor treatment. Protein expression levels were analyzed by Western blot. (D) DEF cells were co-transfected with duck pIFNβ-Luc, pRL-TK, and STING-Myc for 12 h, then infected with DPV-CHv-GFP at an MOI of 1 for 24 h. Luciferase activity was measured in cell lysates using a dual-luciferase assay system. (E-G) DEF cells transfected with STING-Myc for 12 h were infected with DPV-CHv-GFP at an MOI of 1 for 24 h. Total RNA was extracted, and mRNA levels of IFNβ (E), MX (F), and OASL (G) were quantified by qPCR. (H and I) DEF cells were transfected with shNC or shSTING for 12 h and then infected with DPV-CHv-GFP at an MOI of 1, followed by treatment with 10 μM EerI for 24 h. DMSO was used as a control. (H) The viral fluorescent plaques of DPV-CHv-GFP were observed under a fluorescence microscope (scale bar: 50 μm). (I) The cell culture supernatant was collected to determine the TCID 50 . Data are representative of three independent experiments.

Article Snippet: Eeyarestatin I (EerI) (HY-110078), MG132 (HY-13259), Bafilomycin A1 (BAF A1) (HY-100558), Cycloheximide (CHX) (HY-12320) were purchased from Med Chem Express and diluted in dimethyl sulfoxide (DMSO).

Techniques: Transfection, Infection, Expressing, Western Blot, Luciferase, Activity Assay, Control, Fluorescence, Microscopy, Cell Culture

Journal: Food Chemistry: Molecular Sciences

Article Title: The Cl1978 gene regulates delphinidin-based anthocyanin accumulation in a rare purple Wampee ( Clausena lansium )

doi: 10.1016/j.fochms.2026.100389

Figure Lengend Snippet: Anthocyanin proportion (A) and change (B) of purple wampee during ripening. Note: Others in the include 22 minor anthocyanins: cyanidin, cyanidin-3-arabinoside, cyanidin-3-O-galactoside, cyanidin-3-O-glucoside, delphinidin-3-arabinoside, malvidin, malvidin-3-arabinoside, malvidin-3-O-galactoside, malvidin-3-O-glucoside, peonidin, peonidin-3-arabinoside, peonidin 3-galactoside, peonidin-3-O-glucoside, petunidin-3-arabinoside, procyanidin A1, procyanidin A2, procyanidin B1, procyanidin B2, procyanidin B3, procyanidin B4, procyanidin C1, and robinetinidin. Petunidin: Pet; Petunidin-3-O-glucoside: Pet-3-O-glu; Delphinidin: Del; Delphinidin-3-O-glucoside: Del-3-O-glu; Delphinidin 3-O-galactoside: Del-3-O-gal. S1 ∼ S4 represent the immature, unripe, half-ripe, and full-ripe stage of wampee fruits, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Chromatographic-grade procyanidin A1, A2, B1, B2, B3, B4, and C1 (purity ≥98.0%) were supplied by Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China); an additional 32 chromatographic-grade anthocyanidins and anthocyanins (purity ≥95.0%) were supplied by Shanghai ZZBIO Co., Ltd. (Shanghai, China).

Techniques: