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frame insertion asn387 gly388dup tyrosine trna ligase chlc rpld 431632 snp a g missense variant lys63arg 50s ribosomal protein l4 3178132 snp c  (ATCC)
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ATCC frame insertion asn387 gly388dup tyrosine trna ligase chlc rpld 431632 snp a g missense variant lys63arg 50s ribosomal protein l4 3178132 snp c
Frame Insertion Asn387 Gly388dup Tyrosine Trna Ligase Chlc Rpld 431632 Snp A G Missense Variant Lys63arg 50s Ribosomal Protein L4 3178132 Snp C, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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frame insertion asn387 gly388dup tyrosine trna ligase chlc rpld 431632 snp a g missense variant lys63arg 50s ribosomal protein l4 3178132 snp c - by Bioz Stars, 2026-06
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frame insertion  (New England Biolabs)
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New England Biolabs frame insertion
Frame Insertion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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frame atti1 insertion variants  (Twist Bioscience)
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Twist Bioscience frame atti1 insertion variants
(A) Plasmid maps of the cassette capture system. The capture vector, pVR106 (pUS250 <t>-ccdB-attI1-ccdA</t> ) (Table S1), contains a ccdB gene, with an attI1 recombination site embedded within it, under the control of an L-arabinose inducible promoter, allowing counter-selection of successful, site-specific insertion events. Additionally, this plasmid contains the antitoxin gene ccdA under a cumate-inducible promoter to protects cells against the toxic CcdB. Plasmid pVR110 (pttQ- intI1 ) contains intI1 under the control of an IPTG-inducible promoter. (B) Workflow for the recovery and characterisation of the cassette metagenome from different environments. Gene cassettes were amplified from diverse environmental DNA sources (mixed salad leaves, prawn gut, sea water, and horse manure fertiliser), circularised and transformed into recipient cells expressing IntI1. Integration of a cassette into the attI1 site disrupts the ccdB reading frame, ensuring that cells containing a captured cassette survive counter-selection. The resulting library was subjected to two parallel analyses: targeted functional screening to identify AMR genes, and untargeted nanopore sequencing of the PCR-amplified plasmid DNA (using ccdB -in-fow/rev junction primers) to characterise the diversity of the captured cassette pool. Schematic created with BioRender.com and modified using assets from Adobe Stock.
Frame Atti1 Insertion Variants, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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frame atti1 insertion variants - by Bioz Stars, 2026-06
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a-frame insert  (Med Associates Inc)
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Med Associates Inc a-frame insert
(A) Plasmid maps of the cassette capture system. The capture vector, pVR106 (pUS250 <t>-ccdB-attI1-ccdA</t> ) (Table S1), contains a ccdB gene, with an attI1 recombination site embedded within it, under the control of an L-arabinose inducible promoter, allowing counter-selection of successful, site-specific insertion events. Additionally, this plasmid contains the antitoxin gene ccdA under a cumate-inducible promoter to protects cells against the toxic CcdB. Plasmid pVR110 (pttQ- intI1 ) contains intI1 under the control of an IPTG-inducible promoter. (B) Workflow for the recovery and characterisation of the cassette metagenome from different environments. Gene cassettes were amplified from diverse environmental DNA sources (mixed salad leaves, prawn gut, sea water, and horse manure fertiliser), circularised and transformed into recipient cells expressing IntI1. Integration of a cassette into the attI1 site disrupts the ccdB reading frame, ensuring that cells containing a captured cassette survive counter-selection. The resulting library was subjected to two parallel analyses: targeted functional screening to identify AMR genes, and untargeted nanopore sequencing of the PCR-amplified plasmid DNA (using ccdB -in-fow/rev junction primers) to characterise the diversity of the captured cassette pool. Schematic created with BioRender.com and modified using assets from Adobe Stock.
A Frame Insert, supplied by Med Associates Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/a-frame insert/product/Med Associates Inc
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a-frame insert - by Bioz Stars, 2026-06
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insert fragment of the ha-tagged pdcd4 open reading frame  (GenScript corporation)
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GenScript corporation insert fragment of the ha-tagged pdcd4 open reading frame
(A) Plasmid maps of the cassette capture system. The capture vector, pVR106 (pUS250 <t>-ccdB-attI1-ccdA</t> ) (Table S1), contains a ccdB gene, with an attI1 recombination site embedded within it, under the control of an L-arabinose inducible promoter, allowing counter-selection of successful, site-specific insertion events. Additionally, this plasmid contains the antitoxin gene ccdA under a cumate-inducible promoter to protects cells against the toxic CcdB. Plasmid pVR110 (pttQ- intI1 ) contains intI1 under the control of an IPTG-inducible promoter. (B) Workflow for the recovery and characterisation of the cassette metagenome from different environments. Gene cassettes were amplified from diverse environmental DNA sources (mixed salad leaves, prawn gut, sea water, and horse manure fertiliser), circularised and transformed into recipient cells expressing IntI1. Integration of a cassette into the attI1 site disrupts the ccdB reading frame, ensuring that cells containing a captured cassette survive counter-selection. The resulting library was subjected to two parallel analyses: targeted functional screening to identify AMR genes, and untargeted nanopore sequencing of the PCR-amplified plasmid DNA (using ccdB -in-fow/rev junction primers) to characterise the diversity of the captured cassette pool. Schematic created with BioRender.com and modified using assets from Adobe Stock.
Insert Fragment Of The Ha Tagged Pdcd4 Open Reading Frame, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/insert fragment of the ha-tagged pdcd4 open reading frame/product/GenScript corporation
Average 90 stars, based on 1 article reviews
insert fragment of the ha-tagged pdcd4 open reading frame - by Bioz Stars, 2026-06
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maxisorp plates frames with inserts no 469914  (Thermo Fisher)
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Thermo Fisher maxisorp plates frames with inserts no 469914
(A) Plasmid maps of the cassette capture system. The capture vector, pVR106 (pUS250 <t>-ccdB-attI1-ccdA</t> ) (Table S1), contains a ccdB gene, with an attI1 recombination site embedded within it, under the control of an L-arabinose inducible promoter, allowing counter-selection of successful, site-specific insertion events. Additionally, this plasmid contains the antitoxin gene ccdA under a cumate-inducible promoter to protects cells against the toxic CcdB. Plasmid pVR110 (pttQ- intI1 ) contains intI1 under the control of an IPTG-inducible promoter. (B) Workflow for the recovery and characterisation of the cassette metagenome from different environments. Gene cassettes were amplified from diverse environmental DNA sources (mixed salad leaves, prawn gut, sea water, and horse manure fertiliser), circularised and transformed into recipient cells expressing IntI1. Integration of a cassette into the attI1 site disrupts the ccdB reading frame, ensuring that cells containing a captured cassette survive counter-selection. The resulting library was subjected to two parallel analyses: targeted functional screening to identify AMR genes, and untargeted nanopore sequencing of the PCR-amplified plasmid DNA (using ccdB -in-fow/rev junction primers) to characterise the diversity of the captured cassette pool. Schematic created with BioRender.com and modified using assets from Adobe Stock.
Maxisorp Plates Frames With Inserts No 469914, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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maxisorp plates frames with inserts no 469914 - by Bioz Stars, 2026-06
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sticky silicon frame culture-inserts  (ibidi GmbH)
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ibidi GmbH sticky silicon frame culture-inserts
(A) Plasmid maps of the cassette capture system. The capture vector, pVR106 (pUS250 <t>-ccdB-attI1-ccdA</t> ) (Table S1), contains a ccdB gene, with an attI1 recombination site embedded within it, under the control of an L-arabinose inducible promoter, allowing counter-selection of successful, site-specific insertion events. Additionally, this plasmid contains the antitoxin gene ccdA under a cumate-inducible promoter to protects cells against the toxic CcdB. Plasmid pVR110 (pttQ- intI1 ) contains intI1 under the control of an IPTG-inducible promoter. (B) Workflow for the recovery and characterisation of the cassette metagenome from different environments. Gene cassettes were amplified from diverse environmental DNA sources (mixed salad leaves, prawn gut, sea water, and horse manure fertiliser), circularised and transformed into recipient cells expressing IntI1. Integration of a cassette into the attI1 site disrupts the ccdB reading frame, ensuring that cells containing a captured cassette survive counter-selection. The resulting library was subjected to two parallel analyses: targeted functional screening to identify AMR genes, and untargeted nanopore sequencing of the PCR-amplified plasmid DNA (using ccdB -in-fow/rev junction primers) to characterise the diversity of the captured cassette pool. Schematic created with BioRender.com and modified using assets from Adobe Stock.
Sticky Silicon Frame Culture Inserts, supplied by ibidi GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sticky silicon frame culture-inserts/product/ibidi GmbH
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sticky silicon frame culture-inserts - by Bioz Stars, 2026-06
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stage insert h201 k frame slim profile model  (Okolab USA Inc)
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Okolab USA Inc stage insert h201 k frame slim profile model
(A) Plasmid maps of the cassette capture system. The capture vector, pVR106 (pUS250 <t>-ccdB-attI1-ccdA</t> ) (Table S1), contains a ccdB gene, with an attI1 recombination site embedded within it, under the control of an L-arabinose inducible promoter, allowing counter-selection of successful, site-specific insertion events. Additionally, this plasmid contains the antitoxin gene ccdA under a cumate-inducible promoter to protects cells against the toxic CcdB. Plasmid pVR110 (pttQ- intI1 ) contains intI1 under the control of an IPTG-inducible promoter. (B) Workflow for the recovery and characterisation of the cassette metagenome from different environments. Gene cassettes were amplified from diverse environmental DNA sources (mixed salad leaves, prawn gut, sea water, and horse manure fertiliser), circularised and transformed into recipient cells expressing IntI1. Integration of a cassette into the attI1 site disrupts the ccdB reading frame, ensuring that cells containing a captured cassette survive counter-selection. The resulting library was subjected to two parallel analyses: targeted functional screening to identify AMR genes, and untargeted nanopore sequencing of the PCR-amplified plasmid DNA (using ccdB -in-fow/rev junction primers) to characterise the diversity of the captured cassette pool. Schematic created with BioRender.com and modified using assets from Adobe Stock.
Stage Insert H201 K Frame Slim Profile Model, supplied by Okolab USA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stage insert h201 k frame slim profile model/product/Okolab USA Inc
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stage insert h201 k frame slim profile model - by Bioz Stars, 2026-06
90/100 stars
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insert fragment of the hatagged f13b open reading frame  (GenScript corporation)
90
GenScript corporation insert fragment of the hatagged f13b open reading frame
(A) Plasmid maps of the cassette capture system. The capture vector, pVR106 (pUS250 <t>-ccdB-attI1-ccdA</t> ) (Table S1), contains a ccdB gene, with an attI1 recombination site embedded within it, under the control of an L-arabinose inducible promoter, allowing counter-selection of successful, site-specific insertion events. Additionally, this plasmid contains the antitoxin gene ccdA under a cumate-inducible promoter to protects cells against the toxic CcdB. Plasmid pVR110 (pttQ- intI1 ) contains intI1 under the control of an IPTG-inducible promoter. (B) Workflow for the recovery and characterisation of the cassette metagenome from different environments. Gene cassettes were amplified from diverse environmental DNA sources (mixed salad leaves, prawn gut, sea water, and horse manure fertiliser), circularised and transformed into recipient cells expressing IntI1. Integration of a cassette into the attI1 site disrupts the ccdB reading frame, ensuring that cells containing a captured cassette survive counter-selection. The resulting library was subjected to two parallel analyses: targeted functional screening to identify AMR genes, and untargeted nanopore sequencing of the PCR-amplified plasmid DNA (using ccdB -in-fow/rev junction primers) to characterise the diversity of the captured cassette pool. Schematic created with BioRender.com and modified using assets from Adobe Stock.
Insert Fragment Of The Hatagged F13b Open Reading Frame, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/insert fragment of the hatagged f13b open reading frame/product/GenScript corporation
Average 90 stars, based on 1 article reviews
insert fragment of the hatagged f13b open reading frame - by Bioz Stars, 2026-06
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(A) Plasmid maps of the cassette capture system. The capture vector, pVR106 (pUS250 -ccdB-attI1-ccdA ) (Table S1), contains a ccdB gene, with an attI1 recombination site embedded within it, under the control of an L-arabinose inducible promoter, allowing counter-selection of successful, site-specific insertion events. Additionally, this plasmid contains the antitoxin gene ccdA under a cumate-inducible promoter to protects cells against the toxic CcdB. Plasmid pVR110 (pttQ- intI1 ) contains intI1 under the control of an IPTG-inducible promoter. (B) Workflow for the recovery and characterisation of the cassette metagenome from different environments. Gene cassettes were amplified from diverse environmental DNA sources (mixed salad leaves, prawn gut, sea water, and horse manure fertiliser), circularised and transformed into recipient cells expressing IntI1. Integration of a cassette into the attI1 site disrupts the ccdB reading frame, ensuring that cells containing a captured cassette survive counter-selection. The resulting library was subjected to two parallel analyses: targeted functional screening to identify AMR genes, and untargeted nanopore sequencing of the PCR-amplified plasmid DNA (using ccdB -in-fow/rev junction primers) to characterise the diversity of the captured cassette pool. Schematic created with BioRender.com and modified using assets from Adobe Stock.

Journal: bioRxiv

Article Title: Discovery of novel antimicrobial resistance genes in food and fertiliser using a high-throughput gene capture and functional screening platform

doi: 10.64898/2026.03.15.711940

Figure Lengend Snippet: (A) Plasmid maps of the cassette capture system. The capture vector, pVR106 (pUS250 -ccdB-attI1-ccdA ) (Table S1), contains a ccdB gene, with an attI1 recombination site embedded within it, under the control of an L-arabinose inducible promoter, allowing counter-selection of successful, site-specific insertion events. Additionally, this plasmid contains the antitoxin gene ccdA under a cumate-inducible promoter to protects cells against the toxic CcdB. Plasmid pVR110 (pttQ- intI1 ) contains intI1 under the control of an IPTG-inducible promoter. (B) Workflow for the recovery and characterisation of the cassette metagenome from different environments. Gene cassettes were amplified from diverse environmental DNA sources (mixed salad leaves, prawn gut, sea water, and horse manure fertiliser), circularised and transformed into recipient cells expressing IntI1. Integration of a cassette into the attI1 site disrupts the ccdB reading frame, ensuring that cells containing a captured cassette survive counter-selection. The resulting library was subjected to two parallel analyses: targeted functional screening to identify AMR genes, and untargeted nanopore sequencing of the PCR-amplified plasmid DNA (using ccdB -in-fow/rev junction primers) to characterise the diversity of the captured cassette pool. Schematic created with BioRender.com and modified using assets from Adobe Stock.

Article Snippet: A series of in-frame attI1 insertion variants were synthesised (Twist Bioscience, USA) to identify integration sites that preserved marker lethality (Table S2).

Techniques: Plasmid Preparation, Control, Selection, Amplification, Transformation Assay, Expressing, Functional Assay, Nanopore Sequencing, Modification

(A) Captured gene cassette arrays and their corresponding environmental sources. Long arrows indicate open reading frames (ORFs) and their transcriptional orientation, maroon arrows represent attC sites and short green arrows represent the attI1 site. (B) Antimicrobial resistance (AMR) profile of bleomycin resistance genes, AlphaFold3 model of IblA and structural superimposition of IblA (blue) onto its structural homolog AF-Q53795-F1 (yellow). (C) AMR profile of ImzA, AlphaFold3 model and structural superimposition of ImzA (blue) onto its structural homolog AF-A0A0S8E1S1-F1 (yellow). (D) AMR profiles for the known AMR-conferring genes (in parentheses): rifampicin ( arr-3 ), aminoglycoside acetyltransferase ( aacA6 ), and trimethoprim ( dfrA1 ). Bar charts display the fold-increase in minimum inhibitory concentration (MIC) relative to the negative control (pVR106- fuGFP ) (Table S1). The antimicrobial concentrations (µg/mL) are indicated above each bar, and biological replicates (n = 3) are represented by asterisks.

Journal: bioRxiv

Article Title: Discovery of novel antimicrobial resistance genes in food and fertiliser using a high-throughput gene capture and functional screening platform

doi: 10.64898/2026.03.15.711940

Figure Lengend Snippet: (A) Captured gene cassette arrays and their corresponding environmental sources. Long arrows indicate open reading frames (ORFs) and their transcriptional orientation, maroon arrows represent attC sites and short green arrows represent the attI1 site. (B) Antimicrobial resistance (AMR) profile of bleomycin resistance genes, AlphaFold3 model of IblA and structural superimposition of IblA (blue) onto its structural homolog AF-Q53795-F1 (yellow). (C) AMR profile of ImzA, AlphaFold3 model and structural superimposition of ImzA (blue) onto its structural homolog AF-A0A0S8E1S1-F1 (yellow). (D) AMR profiles for the known AMR-conferring genes (in parentheses): rifampicin ( arr-3 ), aminoglycoside acetyltransferase ( aacA6 ), and trimethoprim ( dfrA1 ). Bar charts display the fold-increase in minimum inhibitory concentration (MIC) relative to the negative control (pVR106- fuGFP ) (Table S1). The antimicrobial concentrations (µg/mL) are indicated above each bar, and biological replicates (n = 3) are represented by asterisks.

Article Snippet: A series of in-frame attI1 insertion variants were synthesised (Twist Bioscience, USA) to identify integration sites that preserved marker lethality (Table S2).

Techniques: Concentration Assay, Negative Control