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Correlation of MTAP expression by IHC and <t>9p21</t> copy number by NGS and <t>CDKN2A</t> FISH. (A) OncoPrint of histologic subtype, tumor purity (light red bars: ≤20%), MTAP expression (clones mAb 1813 and EPR6893), 9p21 copy number (CN) determined by integer copy number by FACETS, and detection of CDKN2A homozygous deletion by FISH; see for further details. (B) Contingency tables of MTAP loss using mAb 1813 and 9p21 homozygous deletion (Homdel) by FACETS and/or FISH in the entire study cohort (top), cases with tumor purity >20% (homozygous deletion by FACETS only) and cases with tumor purity ≤20% (homozygous deletion by FISH only) (bottom). FACETS, Fraction and Allele-Specific Copy Number Estimates from Tumor Sequencing; FISH, fluorescence in situ hybridization; mAb, monoclonal antibodies.
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Correlation of MTAP expression by IHC and <t>9p21</t> copy number by NGS and <t>CDKN2A</t> FISH. (A) OncoPrint of histologic subtype, tumor purity (light red bars: ≤20%), MTAP expression (clones mAb 1813 and EPR6893), 9p21 copy number (CN) determined by integer copy number by FACETS, and detection of CDKN2A homozygous deletion by FISH; see for further details. (B) Contingency tables of MTAP loss using mAb 1813 and 9p21 homozygous deletion (Homdel) by FACETS and/or FISH in the entire study cohort (top), cases with tumor purity >20% (homozygous deletion by FACETS only) and cases with tumor purity ≤20% (homozygous deletion by FISH only) (bottom). FACETS, Fraction and Allele-Specific Copy Number Estimates from Tumor Sequencing; FISH, fluorescence in situ hybridization; mAb, monoclonal antibodies.
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Correlation of MTAP expression by IHC and <t>9p21</t> copy number by NGS and <t>CDKN2A</t> FISH. (A) OncoPrint of histologic subtype, tumor purity (light red bars: ≤20%), MTAP expression (clones mAb 1813 and EPR6893), 9p21 copy number (CN) determined by integer copy number by FACETS, and detection of CDKN2A homozygous deletion by FISH; see for further details. (B) Contingency tables of MTAP loss using mAb 1813 and 9p21 homozygous deletion (Homdel) by FACETS and/or FISH in the entire study cohort (top), cases with tumor purity >20% (homozygous deletion by FACETS only) and cases with tumor purity ≤20% (homozygous deletion by FISH only) (bottom). FACETS, Fraction and Allele-Specific Copy Number Estimates from Tumor Sequencing; FISH, fluorescence in situ hybridization; mAb, monoclonal antibodies.
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MTAP loss in tumors lacking <t>9p21</t> deletion. MTAP staining was completely absent in tumor cells of a NET of the lung (A), a NET of the ileum (B), a colorectal NET (C), a pancreatic NET (D), a T-cell non-Hodgkin lymphoma (E), a marginal cell lymphoma (F) and in 2 cases of Hodgkin lymphoma (G and H). Distinct MTAP positivity of intermingled stroma cells serves as a positive internal control.
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Image Search Results


Correlation of MTAP expression by IHC and 9p21 copy number by NGS and CDKN2A FISH. (A) OncoPrint of histologic subtype, tumor purity (light red bars: ≤20%), MTAP expression (clones mAb 1813 and EPR6893), 9p21 copy number (CN) determined by integer copy number by FACETS, and detection of CDKN2A homozygous deletion by FISH; see for further details. (B) Contingency tables of MTAP loss using mAb 1813 and 9p21 homozygous deletion (Homdel) by FACETS and/or FISH in the entire study cohort (top), cases with tumor purity >20% (homozygous deletion by FACETS only) and cases with tumor purity ≤20% (homozygous deletion by FISH only) (bottom). FACETS, Fraction and Allele-Specific Copy Number Estimates from Tumor Sequencing; FISH, fluorescence in situ hybridization; mAb, monoclonal antibodies.

Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Article Title: Comparison of Immunohistochemistry, Next-generation Sequencing and Fluorescence In Situ Hybridization for Detection of MTAP Loss in Pleural Mesothelioma

doi: 10.1016/j.modpat.2023.100420

Figure Lengend Snippet: Correlation of MTAP expression by IHC and 9p21 copy number by NGS and CDKN2A FISH. (A) OncoPrint of histologic subtype, tumor purity (light red bars: ≤20%), MTAP expression (clones mAb 1813 and EPR6893), 9p21 copy number (CN) determined by integer copy number by FACETS, and detection of CDKN2A homozygous deletion by FISH; see for further details. (B) Contingency tables of MTAP loss using mAb 1813 and 9p21 homozygous deletion (Homdel) by FACETS and/or FISH in the entire study cohort (top), cases with tumor purity >20% (homozygous deletion by FACETS only) and cases with tumor purity ≤20% (homozygous deletion by FISH only) (bottom). FACETS, Fraction and Allele-Specific Copy Number Estimates from Tumor Sequencing; FISH, fluorescence in situ hybridization; mAb, monoclonal antibodies.

Article Snippet: CDKN2A (9p21) FISH probe of 222 kb, labeled in orange, was used in combination with a centromere-specific probe for chromosome 9 (CEP9), labeled in green (both from Abbott Molecular).

Techniques: Expressing, Clone Assay, Sequencing, Fluorescence, In Situ Hybridization, Bioprocessing

MTAP loss in tumors lacking 9p21 deletion. MTAP staining was completely absent in tumor cells of a NET of the lung (A), a NET of the ileum (B), a colorectal NET (C), a pancreatic NET (D), a T-cell non-Hodgkin lymphoma (E), a marginal cell lymphoma (F) and in 2 cases of Hodgkin lymphoma (G and H). Distinct MTAP positivity of intermingled stroma cells serves as a positive internal control.

Journal: The American Journal of Surgical Pathology

Article Title: Prevalence of S-methyl-5′-thioadenosine Phosphorylase (MTAP) Deficiency in Human Cancer

doi: 10.1097/PAS.0000000000002297

Figure Lengend Snippet: MTAP loss in tumors lacking 9p21 deletion. MTAP staining was completely absent in tumor cells of a NET of the lung (A), a NET of the ileum (B), a colorectal NET (C), a pancreatic NET (D), a T-cell non-Hodgkin lymphoma (E), a marginal cell lymphoma (F) and in 2 cases of Hodgkin lymphoma (G and H). Distinct MTAP positivity of intermingled stroma cells serves as a positive internal control.

Article Snippet: A commercial 315 kilobases (kb) FISH probe spanning the 194 kb region on chromosome 9p21 where the CDKN2A (27 kb) and the MTAP (135 kb) gene localize was utilized for 9p21 copy number detection (ZytoLight SPEC CDKN2A/CEN 9 Dual Color Probe, Zytovision; #Z-2063).

Techniques: Staining, Control

MTAP loss in tumors with homozygous 9p21 deletion. MTAP staining was completely lacking in tumor cells of an (epithelioid) malignant mesothelioma (A), a urothelial carcinoma of the renal pelvis (B), a mucinous ovarian carcinoma (C), a serous high-grade ovarian carcinoma (D), a malignant melanoma (E), a recurrent adenocarcinoma of the prostate (F), a colorectal adenocarcinoma (G), and a ductal adenocarcinoma of the pancreas (H). Distinct MTAP positivity of intermingled stroma cells and of non-neoplastic epithelial cells serves as a positive internal control.

Journal: The American Journal of Surgical Pathology

Article Title: Prevalence of S-methyl-5′-thioadenosine Phosphorylase (MTAP) Deficiency in Human Cancer

doi: 10.1097/PAS.0000000000002297

Figure Lengend Snippet: MTAP loss in tumors with homozygous 9p21 deletion. MTAP staining was completely lacking in tumor cells of an (epithelioid) malignant mesothelioma (A), a urothelial carcinoma of the renal pelvis (B), a mucinous ovarian carcinoma (C), a serous high-grade ovarian carcinoma (D), a malignant melanoma (E), a recurrent adenocarcinoma of the prostate (F), a colorectal adenocarcinoma (G), and a ductal adenocarcinoma of the pancreas (H). Distinct MTAP positivity of intermingled stroma cells and of non-neoplastic epithelial cells serves as a positive internal control.

Article Snippet: A commercial 315 kilobases (kb) FISH probe spanning the 194 kb region on chromosome 9p21 where the CDKN2A (27 kb) and the MTAP (135 kb) gene localize was utilized for 9p21 copy number detection (ZytoLight SPEC CDKN2A/CEN 9 Dual Color Probe, Zytovision; #Z-2063).

Techniques: Staining, Control

Concordance rate of MTAP IHC and 9p21 copy number analysis.

Journal: The American Journal of Surgical Pathology

Article Title: Prevalence of S-methyl-5′-thioadenosine Phosphorylase (MTAP) Deficiency in Human Cancer

doi: 10.1097/PAS.0000000000002297

Figure Lengend Snippet: Concordance rate of MTAP IHC and 9p21 copy number analysis.

Article Snippet: A commercial 315 kilobases (kb) FISH probe spanning the 194 kb region on chromosome 9p21 where the CDKN2A (27 kb) and the MTAP (135 kb) gene localize was utilized for 9p21 copy number detection (ZytoLight SPEC CDKN2A/CEN 9 Dual Color Probe, Zytovision; #Z-2063).

Techniques: