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Fig. 1. (A) Schematic representation of the <t>pGAPM-pccbgl1</t> vector used in the study. The S. <t>cerevisiae</t> YSR[Pccbgl1] and YSR[pGAPM]; K. pastoris DSMZ[Pccbgl1] and DSMZ[pGAPM] strains were transferred onto (B) SC-esculin and (C) SC-cellobiose plates and cultivated at 30 ◦C. Extracellular β-glucosidase activity resulted in (B) the formation of dark zones surrounding the S. cerevisiae YSR[Pccbgl1] and K. pastoris DSMZ[Pccbgl1] strains and (C) enabled the strains to utilise the cellobiose as a carbohydrate source. The S. cerevisiae YSR[pGAPM] and K. pastoris DSMZ[pGAPM] reference strains do not display β-glucosidase activity and is unable to grow on cellobiose.
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Fig. 1. (A) Schematic representation of the pGAPM-pccbgl1 vector used in the study. The S. cerevisiae YSR[Pccbgl1] and YSR[pGAPM]; K. pastoris DSMZ[Pccbgl1] and DSMZ[pGAPM] strains were transferred onto (B) SC-esculin and (C) SC-cellobiose plates and cultivated at 30 ◦C. Extracellular β-glucosidase activity resulted in (B) the formation of dark zones surrounding the S. cerevisiae YSR[Pccbgl1] and K. pastoris DSMZ[Pccbgl1] strains and (C) enabled the strains to utilise the cellobiose as a carbohydrate source. The S. cerevisiae YSR[pGAPM] and K. pastoris DSMZ[pGAPM] reference strains do not display β-glucosidase activity and is unable to grow on cellobiose.

Journal: Biocatalysis and Agricultural Biotechnology

Article Title: Media optimisation for enhanced β-glucosidase production in Komagataella pastoris

doi: 10.1016/j.bcab.2025.103603

Figure Lengend Snippet: Fig. 1. (A) Schematic representation of the pGAPM-pccbgl1 vector used in the study. The S. cerevisiae YSR[Pccbgl1] and YSR[pGAPM]; K. pastoris DSMZ[Pccbgl1] and DSMZ[pGAPM] strains were transferred onto (B) SC-esculin and (C) SC-cellobiose plates and cultivated at 30 ◦C. Extracellular β-glucosidase activity resulted in (B) the formation of dark zones surrounding the S. cerevisiae YSR[Pccbgl1] and K. pastoris DSMZ[Pccbgl1] strains and (C) enabled the strains to utilise the cellobiose as a carbohydrate source. The S. cerevisiae YSR[pGAPM] and K. pastoris DSMZ[pGAPM] reference strains do not display β-glucosidase activity and is unable to grow on cellobiose.

Article Snippet: The S. cerevisiae YSR[pGAPM] and K. pastoris DSMZ[pGAPM] strains were unable to utilise the cellobiose, whereas the S. cerevisiae YSR[Pccbgl1] and K. pastoris DSMZ[Pccbgl1] strains displayed visible growth after three days of cultivation.

Techniques: Plasmid Preparation, Activity Assay

Fig. 2. The recombinant -■- S. cerevisiae YSR[pGAPM] and -●-YSR[Pccbgl1]; -▴- K. pastoris DSMZ[pGAPM] and -◆- DSMZ[Pccbgl1] strains were cultivated in 2 × SC medium. The (A) biomass production, (B) extracellular volumetric β-glucosidase activity and strain productivity (dashed lines) was monitored daily. (C) SDS-PAGE analysis of the culture supernatants confirmed the presence of the Pccbgl1 as a 120 kDA species for the S. cerevisiae YSR[Pccbgl1] and K. pastoris DSMZ[Pccbgl1] strains. Values represent the mean of three repeats and error bars represent the stan dard deviations.

Journal: Biocatalysis and Agricultural Biotechnology

Article Title: Media optimisation for enhanced β-glucosidase production in Komagataella pastoris

doi: 10.1016/j.bcab.2025.103603

Figure Lengend Snippet: Fig. 2. The recombinant -■- S. cerevisiae YSR[pGAPM] and -●-YSR[Pccbgl1]; -▴- K. pastoris DSMZ[pGAPM] and -◆- DSMZ[Pccbgl1] strains were cultivated in 2 × SC medium. The (A) biomass production, (B) extracellular volumetric β-glucosidase activity and strain productivity (dashed lines) was monitored daily. (C) SDS-PAGE analysis of the culture supernatants confirmed the presence of the Pccbgl1 as a 120 kDA species for the S. cerevisiae YSR[Pccbgl1] and K. pastoris DSMZ[Pccbgl1] strains. Values represent the mean of three repeats and error bars represent the stan dard deviations.

Article Snippet: The S. cerevisiae YSR[pGAPM] and K. pastoris DSMZ[pGAPM] strains were unable to utilise the cellobiose, whereas the S. cerevisiae YSR[Pccbgl1] and K. pastoris DSMZ[Pccbgl1] strains displayed visible growth after three days of cultivation.

Techniques: Recombinant, Activity Assay, SDS Page