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saccharomyces cerevisiae saccharomyces cerevisiae substrate hexose oxidase gene non recombinant control d glucose  (DSMZ)
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116 dsm13335  (DSMZ)
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antibodies u2af2  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc antibodies u2af2
Primer sequences utilized in this study.
Antibodies U2af2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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r777e187 hs ppp1ca  (Addgene inc)
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Primer sequences utilized in this study.
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Image Search Results


Primer sequences utilized in this study.

Journal: Journal of Immunology Research

Article Title: lncRNA ZFAS1 Promotes HMGCR mRNA Stabilization via Binding U2AF2 to Modulate Pancreatic Carcinoma Lipometabolism

doi: 10.1155/2022/4163198

Figure Lengend Snippet: Primer sequences utilized in this study.

Article Snippet: Then, 20 μ g total protein was sampled and separated on SDS-PAGE with a concentration of 10%, followed by transfer onto a PVDF membrane, 1 h of 5% defatted milk blocking, as well as overnight immersion (4°C) in corresponding primary antibodies U2AF2 (1 : 1000, Cell Signaling Technology, USA), HMGCR (1 μ g/mL, Abcam, UK), and β -actin (1 : 1000, Abcam, UK).

Techniques: Sequencing

ZFAS1 maintains HMGCR mRNA stabilization by binding to U2AF2. (a) ZFAS1 distribution in BxPC-3 cells; (b) immunoprecipitation protein levels and RIP assay; (c) U2AF2 and HMGCR expression detected by qRT-PCT; (d) HMGCR mRNA stabilization; (e) HMGCR fold enrichment in U2AF2 relative to IgG; (f) qRT-PCR analysis of HMGCR in RIP experience; ∗ P < 0.05; ∗∗ P < 0.01; ## P < 0.01; ∗∗∗ P < 0.001.

Journal: Journal of Immunology Research

Article Title: lncRNA ZFAS1 Promotes HMGCR mRNA Stabilization via Binding U2AF2 to Modulate Pancreatic Carcinoma Lipometabolism

doi: 10.1155/2022/4163198

Figure Lengend Snippet: ZFAS1 maintains HMGCR mRNA stabilization by binding to U2AF2. (a) ZFAS1 distribution in BxPC-3 cells; (b) immunoprecipitation protein levels and RIP assay; (c) U2AF2 and HMGCR expression detected by qRT-PCT; (d) HMGCR mRNA stabilization; (e) HMGCR fold enrichment in U2AF2 relative to IgG; (f) qRT-PCR analysis of HMGCR in RIP experience; ∗ P < 0.05; ∗∗ P < 0.01; ## P < 0.01; ∗∗∗ P < 0.001.

Article Snippet: Then, 20 μ g total protein was sampled and separated on SDS-PAGE with a concentration of 10%, followed by transfer onto a PVDF membrane, 1 h of 5% defatted milk blocking, as well as overnight immersion (4°C) in corresponding primary antibodies U2AF2 (1 : 1000, Cell Signaling Technology, USA), HMGCR (1 μ g/mL, Abcam, UK), and β -actin (1 : 1000, Abcam, UK).

Techniques: Binding Assay, Immunoprecipitation, Expressing, Quantitative RT-PCR

Impact of U2AF2 on biological function of PC cells. (a) U2AF2 expression; (b) cell viability detection; (c) plate clonal cell formation; (d) cell invasiveness; ∗ P < 0.05; ∗∗ P < 0.01.

Journal: Journal of Immunology Research

Article Title: lncRNA ZFAS1 Promotes HMGCR mRNA Stabilization via Binding U2AF2 to Modulate Pancreatic Carcinoma Lipometabolism

doi: 10.1155/2022/4163198

Figure Lengend Snippet: Impact of U2AF2 on biological function of PC cells. (a) U2AF2 expression; (b) cell viability detection; (c) plate clonal cell formation; (d) cell invasiveness; ∗ P < 0.05; ∗∗ P < 0.01.

Article Snippet: Then, 20 μ g total protein was sampled and separated on SDS-PAGE with a concentration of 10%, followed by transfer onto a PVDF membrane, 1 h of 5% defatted milk blocking, as well as overnight immersion (4°C) in corresponding primary antibodies U2AF2 (1 : 1000, Cell Signaling Technology, USA), HMGCR (1 μ g/mL, Abcam, UK), and β -actin (1 : 1000, Abcam, UK).

Techniques: Expressing