Journal: Scientific Reports

Article Title: Label-free fluorescence lifetime imaging can distinguish cancer from healthy tissue in spontaneously occurring canine oral tumors

doi: 10.1038/s41598-026-37001-3

Figure Lengend Snippet: PpIX accumulation in Canine Epithelial Oral Cancer Cell Lines. ( a ) Confocal microscopy of canine CAA and SCC cell lines with and without incubation with 5-ALA revealing increased mean florescence intensity under excitation at 470 nm when incubated with 5-ALA (+ 5-ALA) compared to the control (-5-ALA, cell autofluorescence only). ( b ): Flow cytometry of canine cell lines with and without 5-ALA incubation showing increased percent of fluorescent cells captured in channel 3, although minimal change was noted in SCC4 compared to other cell lines.

Article Snippet: 5-ALA hydrochloride (Thermo Scientific, Switzerland) was dissolved in phosphate buffered saline and 10% fetal bovine serum and added to cell culture medium at a concentration of 5 mmol/L for 2 h prior to washing and evaluation of PpIX fluorescence as previously described .

Techniques: Confocal Microscopy, Incubation, Control, Flow Cytometry

Journal: Scientific Reports

Article Title: Label-free fluorescence lifetime imaging can distinguish cancer from healthy tissue in spontaneously occurring canine oral tumors

doi: 10.1038/s41598-026-37001-3

Figure Lengend Snippet: Protoporphyrin IX (PpIX) accumulation in canine oral squamous cell carcinoma after exogenous 5-aminolevulinic acid (5-ALA) administration. ( a ) Visual fluorescence (pink) of 5-ALA induced PpIX under blue light stimulation (450 nm) of a canine oral tumor on the hard palate that preferentially accumulated PpIX compared to the surrounding normal tissue. ( b ) Heme biosynthetic pathway illustrating the enzymes involved in breakdown of 5-ALA to PpIX, then heme and bilirubin. * Represents enzymes that were upregulated in fluorescent canine oral squamous cell carcinoma compared to non-fluorescent normal oral mucosa.

Article Snippet: 5-ALA hydrochloride (Thermo Scientific, Switzerland) was dissolved in phosphate buffered saline and 10% fetal bovine serum and added to cell culture medium at a concentration of 5 mmol/L for 2 h prior to washing and evaluation of PpIX fluorescence as previously described .

Techniques: Fluorescence