Journal: Genetics

Article Title: Oxidative Stress Survival in a Clinical Saccharomyces cerevisiae Isolate Is Influenced by a Major Quantitative Trait Nucleotide

doi: 10.1534/genetics.111.128256

Figure Lengend Snippet: Peroxide survival candidate QTL as identified by BSA and SSA. Shown is a combination of all four S98 BSA and 4x2k SSA genotyping analyses. (A) S98 one-mismatch SFPs, (B) S98 two-mismatch SFPs, (C) 4x2k SFPs with gaps, (D) 4x2k ≥3 consecutive SFPs. The black horizontal line at the bottom is the S. cerevisiae genome with its chromosomes indicated by roman numerals between the beginning (left) and end (right) of each chromosome (short vertical bars). Chromosome lengths are drawn to scale. Candidate QTL are marked by short horizontal bars. Red bars represent loci that were found in the most conservative analysis (B) and at least one other analysis. These regions are highlighted by red vertical lines and their approximate position on the chromosome is indicated at the bottom. Black arrows point to candidate QTL that have been tested with diagnostic RFLPs (Table 2).

Article Snippet: SSA via custom-designed Combimatrix 4x2k arrays provides an economic application that facilitates genotyping of segregants of any cross between strains whose genomes have been sequenced.

Techniques: Diagnostic Assay

Journal: Genetics

Article Title: Oxidative Stress Survival in a Clinical Saccharomyces cerevisiae Isolate Is Influenced by a Major Quantitative Trait Nucleotide

doi: 10.1534/genetics.111.128256

Figure Lengend Snippet: Peroxide survival candidate QTL as identified by BSA and SSA. Shown is a combination of all four S98 BSA and 4x2k SSA genotyping analyses. (A) S98 one-mismatch SFPs, (B) S98 two-mismatch SFPs, (C) 4x2k SFPs with gaps, (D) 4x2k ≥3 consecutive SFPs. The black horizontal line at the bottom is the S. cerevisiae genome with its chromosomes indicated by roman numerals between the beginning (left) and end (right) of each chromosome (short vertical bars). Chromosome lengths are drawn to scale. Candidate QTL are marked by short horizontal bars. Red bars represent loci that were found in the most conservative analysis (B) and at least one other analysis. These regions are highlighted by red vertical lines and their approximate position on the chromosome is indicated at the bottom. Black arrows point to candidate QTL that have been tested with diagnostic RFLPs (Table 2).

Article Snippet: The 22 BCI segregants were separately hybridized to the 4x2k Combimatrix custom-designed array and compared to both parental strains.

Techniques: Diagnostic Assay

Journal: Applied and Environmental Microbiology

Article Title: Use of Cellular CRISPR (Clusters of Regularly Interspaced Short Palindromic Repeats) Spacer-Based Microarrays for Detection of Viruses in Environmental Samples ▿ †

doi: 10.1128/AEM.01109-10

Figure Lengend Snippet: Probe category and the number of probes on the two generations of microarrays

Article Snippet: The second generation of microarrays was expanded to include all of the CRISPR spacer sequences derived from CHANN041 and NL10 cellular metagenomes on the CombiMatrix 4X2K custom array.

Techniques: CRISPR