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Weight loss driven improvement of methylation age.

Journal: Frontiers in Endocrinology

Article Title: Implication of DNA methylation during lifestyle mediated weight loss

doi: 10.3389/fendo.2023.1181002

Figure Lengend Snippet: Weight loss driven improvement of methylation age.

Article Snippet: hypocaloric dietary intervention/ 6 weeks Aim: investigate the effects of short-term hypocaloric diet-induced weight loss on DNA methylation profile in leukocytes from women with severe obesity , 11 women with morbid obesity (Age = 36.9 ± 10.3 years; BMI: 58.5 ± 10.5 kg/m 2 ) , Illumina HumanMethylation 450K Bead Chips microarray Tissue: blood , intervention changed the methylation levels at 16,064 CpG sites; These CpGs sites were related to cancer, cell cycle-related, MAPK, Rap1, and Ras signaling pathways; regardless of hypocaloric intervention, a group of 878 CpGs (related to 649 genes) remained significantly altered in obese women when compared with normal-weight women; Pathway enrichment analysis identified genes related to the cadherin and Wnt pathway, angiogenesis signaling, and p53 pathways by glucose deprivation , Nicoletti et al. 2020 ( ) .

Techniques: Methylation, DNA Methylation Assay

In-utero methylation studies under lifestyle intervention.

Journal: Frontiers in Endocrinology

Article Title: Implication of DNA methylation during lifestyle mediated weight loss

doi: 10.3389/fendo.2023.1181002

Figure Lengend Snippet: In-utero methylation studies under lifestyle intervention.

Article Snippet: hypocaloric dietary intervention/ 6 weeks Aim: investigate the effects of short-term hypocaloric diet-induced weight loss on DNA methylation profile in leukocytes from women with severe obesity , 11 women with morbid obesity (Age = 36.9 ± 10.3 years; BMI: 58.5 ± 10.5 kg/m 2 ) , Illumina HumanMethylation 450K Bead Chips microarray Tissue: blood , intervention changed the methylation levels at 16,064 CpG sites; These CpGs sites were related to cancer, cell cycle-related, MAPK, Rap1, and Ras signaling pathways; regardless of hypocaloric intervention, a group of 878 CpGs (related to 649 genes) remained significantly altered in obese women when compared with normal-weight women; Pathway enrichment analysis identified genes related to the cadherin and Wnt pathway, angiogenesis signaling, and p53 pathways by glucose deprivation , Nicoletti et al. 2020 ( ) .

Techniques: Methylation, DNA Methylation Assay, Activity Assay, Control, Modification