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Dual-luciferase reporter assay verified that c-Jun targeted PI3K/AKT. (A) CNE-2R cells cotransfected with the PI3KCG or <t>AKT1</t> promoter construct and TFS-c-Jun were observed by inverted fluorescence microscopy (magnification ×100). (B,C) CNE-2R cells were cotransfected with c-Jun and different PI3KCG or AKT1 promoter-driven luciferase plasmids for 48 hours, and luciferase activity was determined by a luciferase reporter assay kit (*, P<0.05 compared with the control).
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Dual-luciferase reporter assay verified that c-Jun targeted PI3K/AKT. (A) CNE-2R cells cotransfected with the PI3KCG or <t>AKT1</t> promoter construct and TFS-c-Jun were observed by inverted fluorescence microscopy (magnification ×100). (B,C) CNE-2R cells were cotransfected with c-Jun and different PI3KCG or AKT1 promoter-driven luciferase plasmids for 48 hours, and luciferase activity was determined by a luciferase reporter assay kit (*, P<0.05 compared with the control).
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Dual-luciferase reporter assay verified that c-Jun targeted PI3K/AKT. (A) CNE-2R cells cotransfected with the PI3KCG or AKT1 promoter construct and TFS-c-Jun were observed by inverted fluorescence microscopy (magnification ×100). (B,C) CNE-2R cells were cotransfected with c-Jun and different PI3KCG or AKT1 promoter-driven luciferase plasmids for 48 hours, and luciferase activity was determined by a luciferase reporter assay kit (*, P<0.05 compared with the control).

Journal: Annals of Translational Medicine

Article Title: Silencing c-Jun inhibits autophagy and abrogates radioresistance in nasopharyngeal carcinoma by activating the PI3K/AKT/mTOR pathway

doi: 10.21037/atm-21-2563

Figure Lengend Snippet: Dual-luciferase reporter assay verified that c-Jun targeted PI3K/AKT. (A) CNE-2R cells cotransfected with the PI3KCG or AKT1 promoter construct and TFS-c-Jun were observed by inverted fluorescence microscopy (magnification ×100). (B,C) CNE-2R cells were cotransfected with c-Jun and different PI3KCG or AKT1 promoter-driven luciferase plasmids for 48 hours, and luciferase activity was determined by a luciferase reporter assay kit (*, P<0.05 compared with the control).

Article Snippet: Reporter genes, including the promoter AKT1 (42409-1) and promoter PIK3CG (42408-1), were constructed by GeneChem (Shanghai, China), and CNE-2R cells were cotransfected with luciferase reporters and the c-Jun mimic.

Techniques: Luciferase, Reporter Assay, Construct, Fluorescence, Microscopy, Activity Assay, Control