Journal: Journal of advanced research

Article Title: Serum taurine affects lung cancer progression by regulating tumor immune escape mediated by the immune microenvironment.

doi: 10.1016/j.jare.2024.09.005

Figure Lengend Snippet: Fig. 3. Taurine might influence tumor immune response through the Nfe2l1-ROS-PD-1 axis in immune-competent mice. (A-B) RNA sequencing revealed GO analyses and 100 DEGs in subcutaneous lung tumors of C57 mice induced by different doses of taurine (n = 3). (C) Seven representative DEGs were finally identified among the taurine low, taurine high and model group. (D) Different taurine doses significantly affected the expression of Nfe2l1, 41BBL, and PD-1 proteins in tumors of C57 mice, as determined by western blot assay (n = 4). (E) For in vivo experiments, LLC (wt) or LLC (Nfe2l1 overexpression) cells were injected by subcutaneously into wild type C57 mice (5 105 cells per mouse, n = 6). (F) Overexpressed Nfe2l1 gene did not significantly affect mouse body weight. (G) Overexpressed Nfe2l1 gene could significantly inhibit LLC-xenografted tumor growth in C57 mice. (H) Representative images of tumors revealed significant anti-tumor effects of overexpressed Nfe2l1 gene in C57 mice. (I) Overexpressed Nfe2l1 gene could cause a decrease in tumor weight. (J) Overexpressed Nfe2l1 gene significantly affected the expression of Nfe2l1, 41BBL, and PD-1 proteins in tumors of C57 mice (n = 4). Statistical significance was calculated by means of ANOVA analysis, *p < 0.05, **p < 0.01, and ***p < 0.001.

Article Snippet: The following antibodies were used in this experiment: b-Actin (CST#8457), Nfe2l1 (Nrf1, SANTA: sc-28379), 41BBL (CD137L, SANTA: sc-398933), PD-1 (CST#84651), NF-jB (CST#8242), and p-NF-jB (CST#3033).

Techniques: RNA Sequencing, Expressing, Western Blot, In Vivo, Over Expression, Injection

Journal: Cell reports

Article Title: Batf3 + DCs and the 4–1BB/4–1BBL axis are required at the effector phase in the tumor microenvironment for PD-1/PD-L1 blockade efficacy

doi: 10.1016/j.celrep.2024.114141

Figure Lengend Snippet:

Article Snippet: CD137L (41BBL) Biotin , eBioscience , RRID: AB_466788.

Techniques: Purification, Blocking Assay, Control, Recombinant, Staining, Labeling, Software, Cell Analysis