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Alternative splicing isoform analysis via primer extension. All classifications of splicing can be analyzed including exon insertion ( a ), exon deletion ( b ), 3′ splicing acceptor ( c ), 5′ splicing donor ( d ) and mutually exclusive exons ( e ). Exon lengths are not to scale. Primer extension assays of respective genes were assayed on a <t>MALDI-TOF</t> MS. In the mass spectra, extension primer refers to unextended primers, while the subsequent peaks indicate the primer with an extension of the base(s) specified.
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Alternative splicing isoform analysis via primer extension. All classifications of splicing can be analyzed including exon insertion ( a ), exon deletion ( b ), 3′ splicing acceptor ( c ), 5′ splicing donor ( d ) and mutually exclusive exons ( e ). Exon lengths are not to scale. Primer extension assays of respective genes were assayed on a <t>MALDI-TOF</t> MS. In the mass spectra, extension primer refers to unextended primers, while the subsequent peaks indicate the primer with an extension of the base(s) specified.
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Alternative splicing isoform analysis via primer extension. All classifications of splicing can be analyzed including exon insertion ( a ), exon deletion ( b ), 3′ splicing acceptor ( c ), 5′ splicing donor ( d ) and mutually exclusive exons ( e ). Exon lengths are not to scale. Primer extension assays of respective genes were assayed on a <t>MALDI-TOF</t> MS. In the mass spectra, extension primer refers to unextended primers, while the subsequent peaks indicate the primer with an extension of the base(s) specified.
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Alternative splicing isoform analysis via primer extension. All classifications of splicing can be analyzed including exon insertion ( a ), exon deletion ( b ), 3′ splicing acceptor ( c ), 5′ splicing donor ( d ) and mutually exclusive exons ( e ). Exon lengths are not to scale. Primer extension assays of respective genes were assayed on a <t>MALDI-TOF</t> MS. In the mass spectra, extension primer refers to unextended primers, while the subsequent peaks indicate the primer with an extension of the base(s) specified.
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Alternative splicing isoform analysis via primer extension. All classifications of splicing can be analyzed including exon insertion ( a ), exon deletion ( b ), 3′ splicing acceptor ( c ), 5′ splicing donor ( d ) and mutually exclusive exons ( e ). Exon lengths are not to scale. Primer extension assays of respective genes were assayed on a <t>MALDI-TOF</t> MS. In the mass spectra, extension primer refers to unextended primers, while the subsequent peaks indicate the primer with an extension of the base(s) specified.
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384 format spectrochip microarray  (Sequenom)
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Alternative splicing isoform analysis via primer extension. All classifications of splicing can be analyzed including exon insertion ( a ), exon deletion ( b ), 3′ splicing acceptor ( c ), 5′ splicing donor ( d ) and mutually exclusive exons ( e ). Exon lengths are not to scale. Primer extension assays of respective genes were assayed on a <t>MALDI-TOF</t> MS. In the mass spectra, extension primer refers to unextended primers, while the subsequent peaks indicate the primer with an extension of the base(s) specified.
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Alternative splicing isoform analysis via primer extension. All classifications of splicing can be analyzed including exon insertion ( a ), exon deletion ( b ), 3′ splicing acceptor ( c ), 5′ splicing donor ( d ) and mutually exclusive exons ( e ). Exon lengths are not to scale. Primer extension assays of respective genes were assayed on a MALDI-TOF MS. In the mass spectra, extension primer refers to unextended primers, while the subsequent peaks indicate the primer with an extension of the base(s) specified.

Journal: Nucleic Acids Research

Article Title: High-throughput alternative splicing quantification by primer extension and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

doi: 10.1093/nar/gni098

Figure Lengend Snippet: Alternative splicing isoform analysis via primer extension. All classifications of splicing can be analyzed including exon insertion ( a ), exon deletion ( b ), 3′ splicing acceptor ( c ), 5′ splicing donor ( d ) and mutually exclusive exons ( e ). Exon lengths are not to scale. Primer extension assays of respective genes were assayed on a MALDI-TOF MS. In the mass spectra, extension primer refers to unextended primers, while the subsequent peaks indicate the primer with an extension of the base(s) specified.

Article Snippet: ASV analysis was performed using the MassARRAY system (Sequenom) by dispensing ≈10 nl of final product onto a 384-plate format MALDI-TOF MS SpectroCHIP using a SpectroPOINT nanodispenser (Sequenom).

Techniques: