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2de experiment  (ProteomeTech Inc)
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ProteomeTech Inc 2de experiment
Gene expression changes in PPs-treated M. tuberculosis H37Rv. ( A ) Two-dimensional gel electrophoresis <t>(2DE)</t> analysis for protein expression in M. tuberculosis after treatment with 1× MIC and 10× MIC PP1S at 37 °C for 6 h. ( B ) Protein spots showing significantly different expressions between PP1S treated and untreated groups were identified. Two spots whose expressions were significantly increased by PP1S were found to be Rv0560c. ( C ) As a result of confirming the gene transcription pattern through microarray under the same conditions as 2DE, the most upregulated gene by PPs was Rv0560c. Overexpression of Rv0560c by PPs was confirmed by ( D ) RT-PCR and ( E ) Western blotting using an Rv0560c-specific antibody. ( D , E ) Rv0560c gene was also upregulated by salicylate (SAL). RT-PCR results are expressed as mean ± standard deviation.
2de Experiment, supplied by ProteomeTech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2de experiment - by Bioz Stars, 2026-07
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Gene expression changes in PPs-treated M. tuberculosis H37Rv. ( A ) Two-dimensional gel electrophoresis (2DE) analysis for protein expression in M. tuberculosis after treatment with 1× MIC and 10× MIC PP1S at 37 °C for 6 h. ( B ) Protein spots showing significantly different expressions between PP1S treated and untreated groups were identified. Two spots whose expressions were significantly increased by PP1S were found to be Rv0560c. ( C ) As a result of confirming the gene transcription pattern through microarray under the same conditions as 2DE, the most upregulated gene by PPs was Rv0560c. Overexpression of Rv0560c by PPs was confirmed by ( D ) RT-PCR and ( E ) Western blotting using an Rv0560c-specific antibody. ( D , E ) Rv0560c gene was also upregulated by salicylate (SAL). RT-PCR results are expressed as mean ± standard deviation.

Journal: Antibiotics

Article Title: Increased Susceptibility of Mycobacterium tuberculosis to Ethionamide by Expressing PPs-Induced Rv0560c

doi: 10.3390/antibiotics11101349

Figure Lengend Snippet: Gene expression changes in PPs-treated M. tuberculosis H37Rv. ( A ) Two-dimensional gel electrophoresis (2DE) analysis for protein expression in M. tuberculosis after treatment with 1× MIC and 10× MIC PP1S at 37 °C for 6 h. ( B ) Protein spots showing significantly different expressions between PP1S treated and untreated groups were identified. Two spots whose expressions were significantly increased by PP1S were found to be Rv0560c. ( C ) As a result of confirming the gene transcription pattern through microarray under the same conditions as 2DE, the most upregulated gene by PPs was Rv0560c. Overexpression of Rv0560c by PPs was confirmed by ( D ) RT-PCR and ( E ) Western blotting using an Rv0560c-specific antibody. ( D , E ) Rv0560c gene was also upregulated by salicylate (SAL). RT-PCR results are expressed as mean ± standard deviation.

Article Snippet: 2DE experiment was performed by ProteomeTech (Seoul, Korea) as previously described [ ].

Techniques: Gene Expression, Two-Dimensional Gel Electrophoresis, Electrophoresis, Expressing, Microarray, Over Expression, Reverse Transcription Polymerase Chain Reaction, Western Blot, Standard Deviation