Journal: Veterinary research

Article Title: Establishment of minigenomes for infectious bursal disease virus.

doi: 10.1186/s13567-024-01423-6

Figure Lengend Snippet: Figure 6 IBDV MG activity is independent of eIF4E and eIF4G. A and B HEK293T cells (6-well plate format, 106 cells/well, triplicate) were transfected with shRNA plasmids for 24 h. After shRNA plasmid transfection, the cells were co-transfected with the indicated plasmids. Luciferase assays were performed at 72 h after transfection. The expression of VP1 and VP3 was determined by western blotting with the indicated antibodies. GAPDH was used as a loading control. The data are presented as the means ± SD and are representative of three independent experiments. **, p < 0.01 and *, p < 0.05. C HEK293T cells (6-well plate format, 106 cells/well, triplicate) were transfected with the shRNA plasmids and co-transfected for 24 h with the indicated plasmids. At 72 h, the expression of EGFP was determined by fluorescence microscopy. Scale bar, 200 μm. Magnification, ×10.

Article Snippet: Rabbit anti-eIF4E and anti-eIF4G monoclonal antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Activity Assay, Transfection, shRNA, Plasmid Preparation, Luciferase, Expressing, Western Blot, Control, Fluorescence, Microscopy