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Preparation of a 15 steroid mix solution of steroids.

Journal: MethodsX

Article Title: A practical approach to supported liquid extraction and measurement of 18 steroids in plasma and serum by targeted liquid chromatography tandem mass spectrometry

doi: 10.1016/j.mex.2024.102728

Figure Lengend Snippet: Preparation of a 15 steroid mix solution of steroids.

Article Snippet: All reagents used were of analytical grade or better ( Resource availability section ) cortisol (F), cortisone (E), corticosterone (B), 11-deoxycortisol (S), 21-deoxycortisol (21DF), 11-deoxycorticosterone (11DOC), testosterone (T), androstenedione (A4), 5α-dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), progesterone (P4), pregnenolone (P5), 17-hydroxypregnenolone (17OHP5), 17-hydroxyprogesterone (17OHP4), aldosterone (Aldo), estrone (E1), estradiol (E2), estriol (E3) and DC Mass Spect Gold Serum (MSG4000) were all purchased from Sigma-Aldrich and Cerilliant.

Techniques: Concentration Assay

Working internal standard (WIS) solution preparation in 2 mL or 10 mL batches.

Journal: MethodsX

Article Title: A practical approach to supported liquid extraction and measurement of 18 steroids in plasma and serum by targeted liquid chromatography tandem mass spectrometry

doi: 10.1016/j.mex.2024.102728

Figure Lengend Snippet: Working internal standard (WIS) solution preparation in 2 mL or 10 mL batches.

Article Snippet: All reagents used were of analytical grade or better ( Resource availability section ) cortisol (F), cortisone (E), corticosterone (B), 11-deoxycortisol (S), 21-deoxycortisol (21DF), 11-deoxycorticosterone (11DOC), testosterone (T), androstenedione (A4), 5α-dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), progesterone (P4), pregnenolone (P5), 17-hydroxypregnenolone (17OHP5), 17-hydroxyprogesterone (17OHP4), aldosterone (Aldo), estrone (E1), estradiol (E2), estriol (E3) and DC Mass Spect Gold Serum (MSG4000) were all purchased from Sigma-Aldrich and Cerilliant.

Techniques: Concentration Assay

Calibration standard table detailing volume of calibration standard stock solution ( <xref ref-type= Table 2.2 ) and Working IS solution ( Table 2.3 ) to be added to each standard." width="100%" height="100%">

Journal: MethodsX

Article Title: A practical approach to supported liquid extraction and measurement of 18 steroids in plasma and serum by targeted liquid chromatography tandem mass spectrometry

doi: 10.1016/j.mex.2024.102728

Figure Lengend Snippet: Calibration standard table detailing volume of calibration standard stock solution ( Table 2.2 ) and Working IS solution ( Table 2.3 ) to be added to each standard.

Article Snippet: All reagents used were of analytical grade or better ( Resource availability section ) cortisol (F), cortisone (E), corticosterone (B), 11-deoxycortisol (S), 21-deoxycortisol (21DF), 11-deoxycorticosterone (11DOC), testosterone (T), androstenedione (A4), 5α-dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), progesterone (P4), pregnenolone (P5), 17-hydroxypregnenolone (17OHP5), 17-hydroxyprogesterone (17OHP4), aldosterone (Aldo), estrone (E1), estradiol (E2), estriol (E3) and DC Mass Spect Gold Serum (MSG4000) were all purchased from Sigma-Aldrich and Cerilliant.

Techniques:

Positive Multiple Reaction Monitoring parameters and retention times for each steroid and isotopically labelled internal standard as analysed on a QTrap 6500+. DP - Declustering Potential; CE - Collision Energy; CXP - Collision Cell Exit Potential. Quantifier (1) and qualifier (2) indicated accordingly. RT according to separation on a Kinetex C18 (150 × 2.1 mm; 2.6 µm), 0.3 mL/min methanol/water system. Assigned internal standard for each steroid indicated in final column.

Journal: MethodsX

Article Title: A practical approach to supported liquid extraction and measurement of 18 steroids in plasma and serum by targeted liquid chromatography tandem mass spectrometry

doi: 10.1016/j.mex.2024.102728

Figure Lengend Snippet: Positive Multiple Reaction Monitoring parameters and retention times for each steroid and isotopically labelled internal standard as analysed on a QTrap 6500+. DP - Declustering Potential; CE - Collision Energy; CXP - Collision Cell Exit Potential. Quantifier (1) and qualifier (2) indicated accordingly. RT according to separation on a Kinetex C18 (150 × 2.1 mm; 2.6 µm), 0.3 mL/min methanol/water system. Assigned internal standard for each steroid indicated in final column.

Article Snippet: All reagents used were of analytical grade or better ( Resource availability section ) cortisol (F), cortisone (E), corticosterone (B), 11-deoxycortisol (S), 21-deoxycortisol (21DF), 11-deoxycorticosterone (11DOC), testosterone (T), androstenedione (A4), 5α-dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), progesterone (P4), pregnenolone (P5), 17-hydroxypregnenolone (17OHP5), 17-hydroxyprogesterone (17OHP4), aldosterone (Aldo), estrone (E1), estradiol (E2), estriol (E3) and DC Mass Spect Gold Serum (MSG4000) were all purchased from Sigma-Aldrich and Cerilliant.

Techniques:

Recovery of steroids from gold serum at three concentrations, by automated supported liquid extraction, following dilution with 0.1 % formic acid in water and elution with dichloromethane/isopropanol.

Journal: MethodsX

Article Title: A practical approach to supported liquid extraction and measurement of 18 steroids in plasma and serum by targeted liquid chromatography tandem mass spectrometry

doi: 10.1016/j.mex.2024.102728

Figure Lengend Snippet: Recovery of steroids from gold serum at three concentrations, by automated supported liquid extraction, following dilution with 0.1 % formic acid in water and elution with dichloromethane/isopropanol.

Article Snippet: All reagents used were of analytical grade or better ( Resource availability section ) cortisol (F), cortisone (E), corticosterone (B), 11-deoxycortisol (S), 21-deoxycortisol (21DF), 11-deoxycorticosterone (11DOC), testosterone (T), androstenedione (A4), 5α-dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), progesterone (P4), pregnenolone (P5), 17-hydroxypregnenolone (17OHP5), 17-hydroxyprogesterone (17OHP4), aldosterone (Aldo), estrone (E1), estradiol (E2), estriol (E3) and DC Mass Spect Gold Serum (MSG4000) were all purchased from Sigma-Aldrich and Cerilliant.

Techniques: Extraction

Inter-assay accuracy (%RME) and precision (%RSD) for each steroid following automated supported liquid extraction and LC-MS/MS analysis.

Journal: MethodsX

Article Title: A practical approach to supported liquid extraction and measurement of 18 steroids in plasma and serum by targeted liquid chromatography tandem mass spectrometry

doi: 10.1016/j.mex.2024.102728

Figure Lengend Snippet: Inter-assay accuracy (%RME) and precision (%RSD) for each steroid following automated supported liquid extraction and LC-MS/MS analysis.

Article Snippet: All reagents used were of analytical grade or better ( Resource availability section ) cortisol (F), cortisone (E), corticosterone (B), 11-deoxycortisol (S), 21-deoxycortisol (21DF), 11-deoxycorticosterone (11DOC), testosterone (T), androstenedione (A4), 5α-dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), progesterone (P4), pregnenolone (P5), 17-hydroxypregnenolone (17OHP5), 17-hydroxyprogesterone (17OHP4), aldosterone (Aldo), estrone (E1), estradiol (E2), estriol (E3) and DC Mass Spect Gold Serum (MSG4000) were all purchased from Sigma-Aldrich and Cerilliant.

Techniques: Inter Assay, Extraction

Inter-assay accuracy and precision ( n = 6) of quality control serum at low, medium and high levels, defined in the table,for each QC serum panel following automated supported liquid extraction and LC-MS/MS analysis.

Journal: MethodsX

Article Title: A practical approach to supported liquid extraction and measurement of 18 steroids in plasma and serum by targeted liquid chromatography tandem mass spectrometry

doi: 10.1016/j.mex.2024.102728

Figure Lengend Snippet: Inter-assay accuracy and precision ( n = 6) of quality control serum at low, medium and high levels, defined in the table,for each QC serum panel following automated supported liquid extraction and LC-MS/MS analysis.

Article Snippet: All reagents used were of analytical grade or better ( Resource availability section ) cortisol (F), cortisone (E), corticosterone (B), 11-deoxycortisol (S), 21-deoxycortisol (21DF), 11-deoxycorticosterone (11DOC), testosterone (T), androstenedione (A4), 5α-dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), progesterone (P4), pregnenolone (P5), 17-hydroxypregnenolone (17OHP5), 17-hydroxyprogesterone (17OHP4), aldosterone (Aldo), estrone (E1), estradiol (E2), estriol (E3) and DC Mass Spect Gold Serum (MSG4000) were all purchased from Sigma-Aldrich and Cerilliant.

Techniques: Inter Assay, Control, Extraction