Review



20x loading reagent  (fluidigm)


Bioz Verified Symbol fluidigm is a verified supplier
Bioz Manufacturer Symbol fluidigm manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    fluidigm 20x loading reagent
    20x Loading Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/20x+loading+reagent/Bulk+GT+Sample+Loading+Reagent/pmc12764852-239-34-37
    Average 93 stars, based on 1 article reviews
    20x loading reagent - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    other:

    Article Title: Multiplexed detection of febrile infections using CARMEN.
    Article Snippet: Sample mix: The sample mix contained 1x homemade 10X cleavage buffer (1M Tris-HCl (pH 7.5), 0.1M dithiothreitol, and nuclease-free water), 25.2 U RNase Inhibitor (New England Biolabs), 0.1X ROX reference dye (Invitrogen), 20X loading reagent (Fluidigm), 1 mM rNTPs (New England Biolabs), 9 mM MgCl2 in water, and a 500 nM quenched synthetic fluorescent RNA reporter (FAM/rUrUrUrUrUrUrU/3IABkFQ/; Integrated DNA Technologies) was used for a total volume of 14 μl per reaction.

    Article Title: Multiplexed detection of febrile infections using CARMEN
    Article Snippet: Sample mix: The sample mix contained 1x homemade 10X cleavage buffer (1 M Tris-HCl (pH 7.5), 0.1 M dithiothreitol, and nuclease-free water), 25.2 U RNase Inhibitor (New England Biolabs), 0.1X ROX reference dye (Invitrogen), 20X loading reagent (Fluidigm), 1 mM rNTPs (New England Biolabs), 9 mM MgCl 2 in water, and a 500 nM quenched synthetic fluorescent RNA reporter (FAM/rUrUrUrUrUrUrU/3IABkFQ/; Integrated DNA Technologies) was used for a total volume of 14 μl per reaction.

    Article Title: Multiplexed detection of febrile infections using CARMEN
    Article Snippet: The sample mix contained 1x homemade 10X cleavage buffer (1M Tris-HCl (pH 7.5), 0.1M dithiothreitol, and nuclease-free water), 25.2 U RNase Inhibitor (New England Biolabs), 0.1X ROX reference dye (Invitrogen), 20X loading reagent (Fluidigm), 1 mM rNTPs (New England Biolabs), 9 mM MgCl 2 in water, and a 500 nM quenched synthetic fluorescent RNA reporter (FAM/rUrUrUrUrUrUrU/3IABkFQ/; Integrated DNA Technologies) was used for a total volume of 14 μl per reaction.



    Similar Products

    20x loading reagent  (fluidigm)
    93
    fluidigm 20x loading reagent
    20x Loading Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/20x+loading+reagent/Bulk+GT+Sample+Loading+Reagent/pmc12764852-239-34-37
    Average 93 stars, based on 1 article reviews
    20x loading reagent - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    20x ge sample loading reagent  (fluidigm)
    94
    fluidigm 20x ge sample loading reagent
    20x Ge Sample Loading Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/20x+loading+reagent/GE+Sample+Loading+Reagent/10__21203_slash_rs__3__rs___7825215_slash_v1-207-18-23
    Average 94 stars, based on 1 article reviews
    20x ge sample loading reagent - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    20x dna binding dye sample loading reagent  (fluidigm)
    95
    fluidigm 20x dna binding dye sample loading reagent
    H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum <t>(20x),</t> and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.
    20x Dna Binding Dye Sample Loading Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/20x+loading+reagent/DNA+Binding+Dye/pmc12540166-320-17-24
    Average 95 stars, based on 1 article reviews
    20x dna binding dye sample loading reagent - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    20x ge sample loading 303 reagent  (fluidigm)
    94
    fluidigm 20x ge sample loading 303 reagent
    H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum <t>(20x),</t> and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.
    20x Ge Sample Loading 303 Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/20x+loading+reagent/GE+Sample+Loading+Reagent/pmc10083572__pnas__2212476120__sapp-147-21-27
    Average 94 stars, based on 1 article reviews
    20x ge sample loading 303 reagent - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    g 20x ge sample loading reagent  (fluidigm)
    94
    fluidigm g 20x ge sample loading reagent
    H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum <t>(20x),</t> and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.
    G 20x Ge Sample Loading Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/20x+loading+reagent/GE+Sample+Loading+Reagent/pmc10428101__ew3c00169_si_001-110-18-24
    Average 94 stars, based on 1 article reviews
    g 20x ge sample loading reagent - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum (20x), and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.

    Journal: Frontiers in Immunology

    Article Title: Genetic reinstatement of RIG-I in chickens reveals insights into avian immune evolution and influenza interaction

    doi: 10.3389/fimmu.2025.1680791

    Figure Lengend Snippet: H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum (20x), and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.

    Article Snippet: The reaction was prepared by mixing 2.5 μl TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μl 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μl 20X EvaGreen DNA binding dye (Biotum) and 2 μl of preamplified cDNA.

    Techniques: Infection, Generated, Transgenic Assay, Expressing, Comparison, In Vivo