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2 hydroxy datp  (Jena Bioscience)


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    Structured Review

    Jena Bioscience 2 hydroxy datp
    2 Hydroxy Datp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2+hydroxy+datp/2-Hydroxy-dATP/pm41873764-128-51-52
    Average 94 stars, based on 3 article reviews
    2 hydroxy datp - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Purification:

    Article Title: High-throughput methods enabling random duplications, deletions, or nucleotide-constrained mutagenesis of entire DNA motifs
    Article Snippet: 5 μg/ml of a Nb.BbvCI (New England Biolabs) nicked pMW17 plasmid ( ) was incubated at 37°C in the ExoChase Master Mix ( ) for 120 min. After 60 min, an additional 10 mM DTT was added to the reaction. .. The resulting circular and randomly nicked DNA was purified by DNA-binding column and then added to the NSM Master Mix , containing a nucleotide mixture comprised of 2′-Deoxy-P-nucleoside-5′-O-triphosphate (dPTP) (Biolog Life Science Institute), 2′-Deoxyribavirin-5′-O-triphosphate (dRTP) (Biolog Life Science Institute), 2-Hydroxy-dATP (Jena Bioscience), and 8-Oxo-dGTP (Jena Bioscience), which was incubated at 55°C for 10–30 min. 250 Units/ml of T4 DNA ligase (Thermo Scientific), 10 mM DTT (Sigma–Aldrich) and 1 mM ATP (Thermo Scientific) was added to the NSM reaction, which was incubated for a further 60 min at 25°C, to ligate the DNA-backbone. .. 2.4 Units/ml of Thermolabile Proteinase K (New England Biolabs) were added, and the NSM reaction was incubated at 37°C for 30 min to proteolytically inactivate all enzymes.

    Incubation:

    Article Title: High-throughput methods enabling random duplications, deletions, or nucleotide-constrained mutagenesis of entire DNA motifs
    Article Snippet: 5 μg/ml of a Nb.BbvCI (New England Biolabs) nicked pMW17 plasmid ( ) was incubated at 37°C in the ExoChase Master Mix ( ) for 120 min. After 60 min, an additional 10 mM DTT was added to the reaction. .. The resulting circular and randomly nicked DNA was purified by DNA-binding column and then added to the NSM Master Mix , containing a nucleotide mixture comprised of 2′-Deoxy-P-nucleoside-5′-O-triphosphate (dPTP) (Biolog Life Science Institute), 2′-Deoxyribavirin-5′-O-triphosphate (dRTP) (Biolog Life Science Institute), 2-Hydroxy-dATP (Jena Bioscience), and 8-Oxo-dGTP (Jena Bioscience), which was incubated at 55°C for 10–30 min. 250 Units/ml of T4 DNA ligase (Thermo Scientific), 10 mM DTT (Sigma–Aldrich) and 1 mM ATP (Thermo Scientific) was added to the NSM reaction, which was incubated for a further 60 min at 25°C, to ligate the DNA-backbone. .. 2.4 Units/ml of Thermolabile Proteinase K (New England Biolabs) were added, and the NSM reaction was incubated at 37°C for 30 min to proteolytically inactivate all enzymes.

    other:

    Article Title: High-throughput methods enabling random duplications, deletions, or nucleotide-constrained mutagenesis of entire DNA motifs.
    Article Snippet: Data analysis were done using FlowJo v10.10.0 Software (BD Life Sciences), with gating for intact yeast cells done by FSC-H/SSC-H, singlets by SSC-A/SSC-H, and red auto-fluorescence (561 nm, 615/20 nm) was used to remove compromised yeast cells (FSC-H/Y615-H).



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