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Expression of SRSF1 in the AD mouse model and the sham group. Mice in each group received PBS (sham) or Aβ 1-42 (model) for 14 days. Following the MWM test, mice were euthanised, and hippocampal and cortical tissues were collected. (A) Western blot bands and quantitative analysis of SRSF1 and β-actin expression in (B) the hippocampus and (C) the cortex; n=3/group. (D) Representative immunofluorescence images and (E) quantitative analysis of SRSF1. Scale bar, 50 µm. n=3/group; **P<0.01. AD, Alzheimer's disease; SRSF1, Serine/Arginine Rich Splicing Factor; MWM, Morris water maze.

Journal: Molecular Medicine Reports

Article Title: SRSF1 as a promising biomarker and key player in the pathogenesis of Alzheimer's disease

doi: 10.3892/mmr.2026.13882

Figure Lengend Snippet: Expression of SRSF1 in the AD mouse model and the sham group. Mice in each group received PBS (sham) or Aβ 1-42 (model) for 14 days. Following the MWM test, mice were euthanised, and hippocampal and cortical tissues were collected. (A) Western blot bands and quantitative analysis of SRSF1 and β-actin expression in (B) the hippocampus and (C) the cortex; n=3/group. (D) Representative immunofluorescence images and (E) quantitative analysis of SRSF1. Scale bar, 50 µm. n=3/group; **P<0.01. AD, Alzheimer's disease; SRSF1, Serine/Arginine Rich Splicing Factor; MWM, Morris water maze.

Article Snippet: Aβ 1-42 (MedChemExpress) was solubilised in sterile 0.1 M PBS (pH 7.4) at a concentration of 1 mg/ml, aliquoted, and stored at −20°C.

Techniques: Expressing, Western Blot, Immunofluorescence