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cdk5 1h3  (Cell Signaling Technology Inc)
92
Cell Signaling Technology Inc cdk5 1h3
Chronic palmitate exposure during differentiation disrupts both GSK3β and <t>CDK5</t> signalling in SH-SY5Y cells. A – C Western blotting quantification of GSK3β and GSK3β pS9 in D10 SH-SY5Y cells differentiated in the presence of 20 µM BSA-conjugated palmitic or oleic acid, or an equivalent volume of vehicle. All protein levels are normalised to a GAPDH loading control and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. *** p < 0.001, **** p < 0.0001, 1-way ANOVA with Bonferroni’s post-hoc test. A Total GSK3β. B GSK3β phosphorylated at Ser-9 (pS9 GSK3β), normalised to GSK3β levels. C Representative Western blots. D , E Time course of CDK5 and p35 expressing during differentiation of SH-SY5Y cells under chronic oleate, palmitate or equivalent vehicle exposure. All protein levels are normalised to a GAPDH loading control apart from in C and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. D , E Representative Western blot is shown below each graph. * p < 0.05, *** p < 0.001, 2-way ANOVA with Bonferroni’s post-hoc test
Cdk5 1h3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1h3/CDK5+Mouse+mAb/pmc12657545-19-0-5
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cdk5 1h3 - by Bioz Stars, 2026-09
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2-deoxy-d-(1h3)-glucose  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc 2-deoxy-d-(1h3)-glucose
Chronic palmitate exposure during differentiation disrupts both GSK3β and <t>CDK5</t> signalling in SH-SY5Y cells. A – C Western blotting quantification of GSK3β and GSK3β pS9 in D10 SH-SY5Y cells differentiated in the presence of 20 µM BSA-conjugated palmitic or oleic acid, or an equivalent volume of vehicle. All protein levels are normalised to a GAPDH loading control and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. *** p < 0.001, **** p < 0.0001, 1-way ANOVA with Bonferroni’s post-hoc test. A Total GSK3β. B GSK3β phosphorylated at Ser-9 (pS9 GSK3β), normalised to GSK3β levels. C Representative Western blots. D , E Time course of CDK5 and p35 expressing during differentiation of SH-SY5Y cells under chronic oleate, palmitate or equivalent vehicle exposure. All protein levels are normalised to a GAPDH loading control apart from in C and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. D , E Representative Western blot is shown below each graph. * p < 0.05, *** p < 0.001, 2-way ANOVA with Bonferroni’s post-hoc test
2 Deoxy D (1h3) Glucose, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1h3/radiochemicals/pmc09380596__DataSheet_1-9-8-13
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2-deoxy-d-(1h3)-glucose - by Bioz Stars, 2026-09
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toluene (inchi = 1s/c7h8/c1-7-5-3-2-4-6-7/h2-6h,1h3)  (M. BRAUN)
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M. BRAUN toluene (inchi = 1s/c7h8/c1-7-5-3-2-4-6-7/h2-6h,1h3)
Chronic palmitate exposure during differentiation disrupts both GSK3β and <t>CDK5</t> signalling in SH-SY5Y cells. A – C Western blotting quantification of GSK3β and GSK3β pS9 in D10 SH-SY5Y cells differentiated in the presence of 20 µM BSA-conjugated palmitic or oleic acid, or an equivalent volume of vehicle. All protein levels are normalised to a GAPDH loading control and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. *** p < 0.001, **** p < 0.0001, 1-way ANOVA with Bonferroni’s post-hoc test. A Total GSK3β. B GSK3β phosphorylated at Ser-9 (pS9 GSK3β), normalised to GSK3β levels. C Representative Western blots. D , E Time course of CDK5 and p35 expressing during differentiation of SH-SY5Y cells under chronic oleate, palmitate or equivalent vehicle exposure. All protein levels are normalised to a GAPDH loading control apart from in C and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. D , E Representative Western blot is shown below each graph. * p < 0.05, *** p < 0.001, 2-way ANOVA with Bonferroni’s post-hoc test
Toluene (Inchi = 1s/C7h8/C1 7 5 3 2 4 6 7/H2 6h,1h3), supplied by M. BRAUN, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
toluene (inchi = 1s/c7h8/c1-7-5-3-2-4-6-7/h2-6h,1h3) - by Bioz Stars, 2026-09
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2-deoxy-d-(1h3)glucose  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc 2-deoxy-d-(1h3)glucose
Chronic palmitate exposure during differentiation disrupts both GSK3β and <t>CDK5</t> signalling in SH-SY5Y cells. A – C Western blotting quantification of GSK3β and GSK3β pS9 in D10 SH-SY5Y cells differentiated in the presence of 20 µM BSA-conjugated palmitic or oleic acid, or an equivalent volume of vehicle. All protein levels are normalised to a GAPDH loading control and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. *** p < 0.001, **** p < 0.0001, 1-way ANOVA with Bonferroni’s post-hoc test. A Total GSK3β. B GSK3β phosphorylated at Ser-9 (pS9 GSK3β), normalised to GSK3β levels. C Representative Western blots. D , E Time course of CDK5 and p35 expressing during differentiation of SH-SY5Y cells under chronic oleate, palmitate or equivalent vehicle exposure. All protein levels are normalised to a GAPDH loading control apart from in C and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. D , E Representative Western blot is shown below each graph. * p < 0.05, *** p < 0.001, 2-way ANOVA with Bonferroni’s post-hoc test
2 Deoxy D (1h3)glucose, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1h3/radiochemicals/pmc11156499__10___1055___a___2240___7462___sup_pmh0654-64-1-14
Average 90 stars, based on 1 article reviews
2-deoxy-d-(1h3)glucose - by Bioz Stars, 2026-09
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nf l antibody 1h3 nefl novus biologicals  (Novus Biologicals)
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Novus Biologicals nf l antibody 1h3 nefl novus biologicals
Chronic palmitate exposure during differentiation disrupts both GSK3β and <t>CDK5</t> signalling in SH-SY5Y cells. A – C Western blotting quantification of GSK3β and GSK3β pS9 in D10 SH-SY5Y cells differentiated in the presence of 20 µM BSA-conjugated palmitic or oleic acid, or an equivalent volume of vehicle. All protein levels are normalised to a GAPDH loading control and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. *** p < 0.001, **** p < 0.0001, 1-way ANOVA with Bonferroni’s post-hoc test. A Total GSK3β. B GSK3β phosphorylated at Ser-9 (pS9 GSK3β), normalised to GSK3β levels. C Representative Western blots. D , E Time course of CDK5 and p35 expressing during differentiation of SH-SY5Y cells under chronic oleate, palmitate or equivalent vehicle exposure. All protein levels are normalised to a GAPDH loading control apart from in C and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. D , E Representative Western blot is shown below each graph. * p < 0.05, *** p < 0.001, 2-way ANOVA with Bonferroni’s post-hoc test
Nf L Antibody 1h3 Nefl Novus Biologicals, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1h3/NF-L+Antibody+(1H3)+-+BSA+Free/pmc11058230__41419_2024_6692_MOESM3_ESM-1-215-219
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nf l antibody 1h3 nefl novus biologicals - by Bioz Stars, 2026-09
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anti-b7x (1h3)  (Bio X Cell)
90
Bio X Cell anti-b7x (1h3)
Chronic palmitate exposure during differentiation disrupts both GSK3β and <t>CDK5</t> signalling in SH-SY5Y cells. A – C Western blotting quantification of GSK3β and GSK3β pS9 in D10 SH-SY5Y cells differentiated in the presence of 20 µM BSA-conjugated palmitic or oleic acid, or an equivalent volume of vehicle. All protein levels are normalised to a GAPDH loading control and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. *** p < 0.001, **** p < 0.0001, 1-way ANOVA with Bonferroni’s post-hoc test. A Total GSK3β. B GSK3β phosphorylated at Ser-9 (pS9 GSK3β), normalised to GSK3β levels. C Representative Western blots. D , E Time course of CDK5 and p35 expressing during differentiation of SH-SY5Y cells under chronic oleate, palmitate or equivalent vehicle exposure. All protein levels are normalised to a GAPDH loading control apart from in C and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. D , E Representative Western blot is shown below each graph. * p < 0.05, *** p < 0.001, 2-way ANOVA with Bonferroni’s post-hoc test
Anti B7x (1h3), supplied by Bio X Cell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1h3/anti+b7x++1h3+/pm37963469-394-26-33
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anti-b7x (1h3) - by Bioz Stars, 2026-09
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batf2  (Developmental Studies Hybridoma Bank)
92
Developmental Studies Hybridoma Bank batf2
Chronic palmitate exposure during differentiation disrupts both GSK3β and <t>CDK5</t> signalling in SH-SY5Y cells. A – C Western blotting quantification of GSK3β and GSK3β pS9 in D10 SH-SY5Y cells differentiated in the presence of 20 µM BSA-conjugated palmitic or oleic acid, or an equivalent volume of vehicle. All protein levels are normalised to a GAPDH loading control and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. *** p < 0.001, **** p < 0.0001, 1-way ANOVA with Bonferroni’s post-hoc test. A Total GSK3β. B GSK3β phosphorylated at Ser-9 (pS9 GSK3β), normalised to GSK3β levels. C Representative Western blots. D , E Time course of CDK5 and p35 expressing during differentiation of SH-SY5Y cells under chronic oleate, palmitate or equivalent vehicle exposure. All protein levels are normalised to a GAPDH loading control apart from in C and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. D , E Representative Western blot is shown below each graph. * p < 0.05, *** p < 0.001, 2-way ANOVA with Bonferroni’s post-hoc test
Batf2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1h3/anti-Basic+leucine+zipper+transcription+factor/10__1158_slash_2643___3230__bcd___23___0062-392-8-10
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anti pitrm1  (Novus Biologicals)
91
Novus Biologicals anti pitrm1
Chronic palmitate exposure during differentiation disrupts both GSK3β and <t>CDK5</t> signalling in SH-SY5Y cells. A – C Western blotting quantification of GSK3β and GSK3β pS9 in D10 SH-SY5Y cells differentiated in the presence of 20 µM BSA-conjugated palmitic or oleic acid, or an equivalent volume of vehicle. All protein levels are normalised to a GAPDH loading control and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. *** p < 0.001, **** p < 0.0001, 1-way ANOVA with Bonferroni’s post-hoc test. A Total GSK3β. B GSK3β phosphorylated at Ser-9 (pS9 GSK3β), normalised to GSK3β levels. C Representative Western blots. D , E Time course of CDK5 and p35 expressing during differentiation of SH-SY5Y cells under chronic oleate, palmitate or equivalent vehicle exposure. All protein levels are normalised to a GAPDH loading control apart from in C and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. D , E Representative Western blot is shown below each graph. * p < 0.05, *** p < 0.001, 2-way ANOVA with Bonferroni’s post-hoc test
Anti Pitrm1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1h3/PITRM1+Antibody+(1H3)/pm37286597-721-118-119
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Image Search Results


Chronic palmitate exposure during differentiation disrupts both GSK3β and CDK5 signalling in SH-SY5Y cells. A – C Western blotting quantification of GSK3β and GSK3β pS9 in D10 SH-SY5Y cells differentiated in the presence of 20 µM BSA-conjugated palmitic or oleic acid, or an equivalent volume of vehicle. All protein levels are normalised to a GAPDH loading control and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. *** p < 0.001, **** p < 0.0001, 1-way ANOVA with Bonferroni’s post-hoc test. A Total GSK3β. B GSK3β phosphorylated at Ser-9 (pS9 GSK3β), normalised to GSK3β levels. C Representative Western blots. D , E Time course of CDK5 and p35 expressing during differentiation of SH-SY5Y cells under chronic oleate, palmitate or equivalent vehicle exposure. All protein levels are normalised to a GAPDH loading control apart from in C and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. D , E Representative Western blot is shown below each graph. * p < 0.05, *** p < 0.001, 2-way ANOVA with Bonferroni’s post-hoc test

Journal: Molecular Neurobiology

Article Title: Chronic Fatty Acid Exposure Disrupts SH-SY5Y and Neuronal Differentiation and Is a Potential Link Between Type-2 Diabetes and Alzheimer’s Disease

doi: 10.1007/s12035-025-05367-6

Figure Lengend Snippet: Chronic palmitate exposure during differentiation disrupts both GSK3β and CDK5 signalling in SH-SY5Y cells. A – C Western blotting quantification of GSK3β and GSK3β pS9 in D10 SH-SY5Y cells differentiated in the presence of 20 µM BSA-conjugated palmitic or oleic acid, or an equivalent volume of vehicle. All protein levels are normalised to a GAPDH loading control and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. *** p < 0.001, **** p < 0.0001, 1-way ANOVA with Bonferroni’s post-hoc test. A Total GSK3β. B GSK3β phosphorylated at Ser-9 (pS9 GSK3β), normalised to GSK3β levels. C Representative Western blots. D , E Time course of CDK5 and p35 expressing during differentiation of SH-SY5Y cells under chronic oleate, palmitate or equivalent vehicle exposure. All protein levels are normalised to a GAPDH loading control apart from in C and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. D , E Representative Western blot is shown below each graph. * p < 0.05, *** p < 0.001, 2-way ANOVA with Bonferroni’s post-hoc test

Article Snippet: CDK5 (1H3) , Mouse , Cell Signaling technologies , 12134 , 1:500.

Techniques: Western Blot, Control, Expressing