Journal: Frontiers in Veterinary Science

Article Title: Acetonic Fraction of Bidens pilosa Enriched for Maturase K Is Able to Control Cerebral Parasite Burden in Mice Experimentally Infected With Toxoplasma gondii

doi: 10.3389/fvets.2019.00055

Figure Lengend Snippet: Identification of maturase k in acetonic fraction from Bidens pilosa stained by Coomassie brilliant blue G-250®, resolved by 12% SDS-PAGE. (A) One-dimensional gel electrophoresis (1-DE). (A) Apparent molecular mass (Mr) of the B. pilosa acetonic fraction is shown on the right as determined by Kodak 1D image analysis. Representative gel image from four independent experiments. (B) Two-dimensional gel electrophoresis (2-DE). Isoelectric focusing (IEF) was performed on 7 cm immobilized pH gradient strips (ReadyStrip TM IPG Strip pH 3–10). The protein spot was determined by ImageMasterTM 2D Platinum 7.0 software. The spot is identified by arrow. Molecular weight markers (MW) are indicated on the left in kiloDalton (kDa) and the range of isoelectric point (pI) is indicated above the panel. Representative gel image from two matched independent experiments.

Article Snippet: The molecular weight bands were estimated by linear regression analysis, based on the calculation of relative mobility (Rf), using KODAK 1D Image Analysis program (Eastman Kodak Co., Rochester, USA).

Techniques: Staining, SDS Page, Nucleic Acid Electrophoresis, Two-Dimensional Gel Electrophoresis, Electrophoresis, Stripping Membranes, Software, Molecular Weight