Journal: Animals : an Open Access Journal from MDPI

Article Title: Mitochondrial Haplogroups Influence Mitochondrial Structure and Function, Oxidative Stress, Autophagy, and Lipid Metabolism of Chicken Hepatocytes in Response to Energy Stimulation

doi: 10.3390/ani16050766

Figure Lengend Snippet: The effects of mitochondrial haplogroups on the expression of mitochondria-related proteins in the livers of the refed hens. ( A ) Representative images of some mitochondria-associated proteins in the A-group and E-group chicken hepatocytes from immunoblotting analysis. ( B – F ) Quantification of the content of mitochondria-associated proteins, including MFN2, CYTB, MT-ND1, LC3, and SOD2, in the A-group and E-group chicken hepatocytes. n = 6. Data are presented as the mean ± SD. Statistical significance was assessed using Student’s t -test.

Article Snippet: Primary antibodies used in this study include Mitofusin 2 (MFN2) (sc-100560, Santa Cruz Biotechnology, Dallas, TX, USA), Beta-Actin (β-actin) (AC026, ABclonal, Wuhan, China), Superoxide Dismutase 2 (SOD2) (24127-1-AP, Proteintech, Wuhan, China), Microtubule-Associated Protein 1A/1B-Light Chain 3 Beta (LC3B) (3868T, Cell Signaling Technology, Danvers, MA, USA), Mitochondrially Encoded NADH Dehydrogenase 1 (MT-ND1) (YA2089, MedChemExpress, Monmouth Junction, NJ, USA), and Cytochrome B (CYTB) (55090-1-AP, Proteintech, Wuhan, China).

Techniques: Expressing, Western Blot

Journal: Animals : an Open Access Journal from MDPI

Article Title: Mitochondrial Haplogroups Influence Mitochondrial Structure and Function, Oxidative Stress, Autophagy, and Lipid Metabolism of Chicken Hepatocytes in Response to Energy Stimulation

doi: 10.3390/ani16050766

Figure Lengend Snippet: The effects of mitochondrial haplogroups on the expression of mitochondria-related proteins in the livers of the fasted hens. ( A ) Representative images of some mitochondria-associated proteins in the A-group and E-group chicken hepatocytes from immunoblotting analysis. ( B – F ) Quantification of the content of mitochondria-associated proteins, including MFN2, CYTB, MT-ND1, LC3, and SOD2, in the A-group and E-group chicken hepatocytes. n = 6. Data are presented as the mean ± SD. Statistical significance was assessed using Student’s t -test. * denotes p < 0.05.

Article Snippet: Primary antibodies used in this study include Mitofusin 2 (MFN2) (sc-100560, Santa Cruz Biotechnology, Dallas, TX, USA), Beta-Actin (β-actin) (AC026, ABclonal, Wuhan, China), Superoxide Dismutase 2 (SOD2) (24127-1-AP, Proteintech, Wuhan, China), Microtubule-Associated Protein 1A/1B-Light Chain 3 Beta (LC3B) (3868T, Cell Signaling Technology, Danvers, MA, USA), Mitochondrially Encoded NADH Dehydrogenase 1 (MT-ND1) (YA2089, MedChemExpress, Monmouth Junction, NJ, USA), and Cytochrome B (CYTB) (55090-1-AP, Proteintech, Wuhan, China).

Techniques: Expressing, Western Blot

Journal: Animals : an Open Access Journal from MDPI

Article Title: Mitochondrial Haplogroups Influence Mitochondrial Structure and Function, Oxidative Stress, Autophagy, and Lipid Metabolism of Chicken Hepatocytes in Response to Energy Stimulation

doi: 10.3390/ani16050766

Figure Lengend Snippet: Impact of mitochondrial haplogroups on mitochondrial protein content in chicken primary hepatocytes upon glucose treatment. ( A ) Representative immunoblotting images of some mitochondria-associated proteins in the A-group and E-group chicken hepatocytes. ( B – F ) Quantification of the immunoblots for some mitochondria-associated proteins, including MFN2, CYTB, MT-ND1, LC3, and SOD2, in the A-group and E-group chicken hepatocytes. n = 3. Data are presented as the mean ± SD. Statistical significance was assessed using Student’s t -test. ** denotes p < 0.01.

Article Snippet: Primary antibodies used in this study include Mitofusin 2 (MFN2) (sc-100560, Santa Cruz Biotechnology, Dallas, TX, USA), Beta-Actin (β-actin) (AC026, ABclonal, Wuhan, China), Superoxide Dismutase 2 (SOD2) (24127-1-AP, Proteintech, Wuhan, China), Microtubule-Associated Protein 1A/1B-Light Chain 3 Beta (LC3B) (3868T, Cell Signaling Technology, Danvers, MA, USA), Mitochondrially Encoded NADH Dehydrogenase 1 (MT-ND1) (YA2089, MedChemExpress, Monmouth Junction, NJ, USA), and Cytochrome B (CYTB) (55090-1-AP, Proteintech, Wuhan, China).

Techniques: Western Blot

Journal: Animals : an Open Access Journal from MDPI

Article Title: Mitochondrial Haplogroups Influence Mitochondrial Structure and Function, Oxidative Stress, Autophagy, and Lipid Metabolism of Chicken Hepatocytes in Response to Energy Stimulation

doi: 10.3390/ani16050766

Figure Lengend Snippet: Impact of mitochondrial haplogroups on mitochondrial protein content in chicken primary hepatocytes upon oleic acid treatment. ( A ) Representative immunoblotting images of some mitochondria-associated proteins in the A-group and E-group chicken hepatocytes. ( B – F ) Quantification of immunoblots of some mitochondria-associated proteins, including MFN2, CYTB, MT-ND1, LC3, and SOD2, in the A-group and E-group chicken hepatocytes. n = 3. Data are presented as the mean ± SD. Statistical significance was assessed using Student’s t -test. * denotes p < 0.05, and ** denotes p < 0.01.

Article Snippet: Primary antibodies used in this study include Mitofusin 2 (MFN2) (sc-100560, Santa Cruz Biotechnology, Dallas, TX, USA), Beta-Actin (β-actin) (AC026, ABclonal, Wuhan, China), Superoxide Dismutase 2 (SOD2) (24127-1-AP, Proteintech, Wuhan, China), Microtubule-Associated Protein 1A/1B-Light Chain 3 Beta (LC3B) (3868T, Cell Signaling Technology, Danvers, MA, USA), Mitochondrially Encoded NADH Dehydrogenase 1 (MT-ND1) (YA2089, MedChemExpress, Monmouth Junction, NJ, USA), and Cytochrome B (CYTB) (55090-1-AP, Proteintech, Wuhan, China).

Techniques: Western Blot