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Evaluation of different beta‐casein removal approaches. Beta‐casein bands from Western blot analysis (top) and total protein content determined by ATR‐FTIR (bottom). Control = skimmed HM. Error bars indicate standard deviation ( N = 3). ATR‐FTIR, attenuated total reflectance–Fourier transform infrared; CaCl 2 , calcium chloride; HAc, acetic acid; HCl, hydrochloric acid; KH 2 PO 4 , potassium dihydrogen phosphate.
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Evaluation of different beta‐casein removal approaches. Beta‐casein bands from Western blot analysis (top) and total protein content determined by ATR‐FTIR (bottom). Control = skimmed HM. Error bars indicate standard deviation ( N = 3). ATR‐FTIR, attenuated total reflectance–Fourier transform infrared; CaCl 2 , calcium chloride; HAc, acetic acid; HCl, hydrochloric acid; KH 2 PO 4 , potassium dihydrogen phosphate.

Journal: Journal of Extracellular Biology

Article Title: Workflow for the Isolation and Characterisation of Human Milk Extracellular Vesicles (HMEVs) and Their Inflammatory Biomarker Profile

doi: 10.1002/jex2.70110

Figure Lengend Snippet: Evaluation of different beta‐casein removal approaches. Beta‐casein bands from Western blot analysis (top) and total protein content determined by ATR‐FTIR (bottom). Control = skimmed HM. Error bars indicate standard deviation ( N = 3). ATR‐FTIR, attenuated total reflectance–Fourier transform infrared; CaCl 2 , calcium chloride; HAc, acetic acid; HCl, hydrochloric acid; KH 2 PO 4 , potassium dihydrogen phosphate.

Article Snippet: Primary antibodies against CD81, Alix, β‐casein and secondary antibody against IgG were purchased from Santa Cruz Biotech (USA).

Techniques: Western Blot, Control, Standard Deviation, Fourier Transform Infrared Spectroscopy

(Top) EV concentrations for the four tested processing and storage procedures. Note : Each condition was evaluated using two independent replicates; *one‐way ANOVA with Tukey's post hoc test was used for identifying significant differences (i.e., p values <0.05); EV concentrations were normalised to HM volume. (Bottom) Western blot analysis for the method (from HM‐DP to HMS2‐dUC) and casein positive control. DP, direct processing; dUC, differential ultracentrifugation; EV, extracellular vesicle; HM, human milk; HMS, HM storage.

Journal: Journal of Extracellular Biology

Article Title: Workflow for the Isolation and Characterisation of Human Milk Extracellular Vesicles (HMEVs) and Their Inflammatory Biomarker Profile

doi: 10.1002/jex2.70110

Figure Lengend Snippet: (Top) EV concentrations for the four tested processing and storage procedures. Note : Each condition was evaluated using two independent replicates; *one‐way ANOVA with Tukey's post hoc test was used for identifying significant differences (i.e., p values <0.05); EV concentrations were normalised to HM volume. (Bottom) Western blot analysis for the method (from HM‐DP to HMS2‐dUC) and casein positive control. DP, direct processing; dUC, differential ultracentrifugation; EV, extracellular vesicle; HM, human milk; HMS, HM storage.

Article Snippet: Primary antibodies against CD81, Alix, β‐casein and secondary antibody against IgG were purchased from Santa Cruz Biotech (USA).

Techniques: Western Blot, Positive Control