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    Perrigo Inc cream perrigo n a zombie nir fixable viability kit biolegend
    Cream Perrigo N A Zombie Nir Fixable Viability Kit Biolegend, supplied by Perrigo Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zombie+nir/pm33910004-182-45-46
    Average 86 stars, based on 1 article reviews
    cream perrigo n a zombie nir fixable viability kit biolegend - by Bioz Stars, 2026-11
    86/100 stars

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    Cream:

    Article Title: The miR-424(322)/503 gene cluster regulates pro- versus anti-inflammatory skin DC subset differentiation by modulating TGF-β signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies (clone) Anti-human CD1a (HI149) BD Biosciences Cat# 563938 Anti-human CD83 (HB15e) BD Biosciences Cat# 551073 Anti-human CD80 (L307.4) BD Biosciences Cat# 56113 Anti-human CD86 (FUN-1) BD Biosciences Cat# 561128 Anti-human CD11b (ICRF44) Biolegend Cat# 301306 Anti-human CD207 (DCGM4) Beckman Coulter Cat# IM3577 Anti-human CD209 (eB-h209) eBioscience Cat# 12-2099-42 Anti-human/mouse CD207(929F3.01) Acris Antibodies Cat# DDX0362 Anti-mouse MHC II/ I-A/I-E (M5/114.15.2) Biolegend Cat# 107607 Anti-mouse CD103 (2E7) eBioscience Cat# 50-150-43 Anti-human/mouse CD207 (4c7) Biolegend Cat# 144205 Anti-human/mouse CD11b (M1/70) Biolegend Cat# 101207 Anti-mouse MHC II/ I-A/I-E (M5/114.15.2) Biolegend Cat# 107619 Anti-mouse Ly-6C (HK 1.4) Biolegend Cat# 128017 Anti-mouse CD86 (GL-1) Biolegend Cat# 105013 Anti-mouse CD11c (418) Biolegend Cat# 117325 Anti-mouse CD45 (30-F11) Biolegend Cat# 103108 Anti-mouse EpCAM (G8.8) Biolegend Cat# 118227 Anti-mouse CD64 (290322) R&D Cat# FAB20741c Anti-mouse MerTK (108928) R&D Cat# FAB5912N Anti-mouse CD117/c-kit (2B8) Biolegend Cat# 105815 Anti-mouse CD135 (A2F10) Biolegend Cat# 135309 Anti-mouse CD115/CSF-1R (AFS98) Biolegend Cat# 135531 Anti-mouse CD172a/SIRPa (P84) Biolegend Cat# 144011 Anti-mouse CD16/32 (93) Biolegend Cat# 101301 Bacterial and virus strains MiR Zip-424 anti-miR-424 microRNA construct System Biosciences (SBI) Cat# MZIP424-PA-1 MiR Zip Scramble Hairpin control System Biosciences (SBI) Cat# MZIP000-PA-1 TLR4 agonist - LPS-EK Ultrapure InvivoGen Cat# tlrl-peklps PGN from Staphylococcus aureus Sigma Aldrich Cat# 77140 Chemicals, peptides, and recombinant proteins Recombinant human GM-CSF Peprotech Cat# 300-03 Recombinant human TNF-a Peprotech Cat# 300-01A Recombinant human SCF Peprotech Cat# 300-07 Recombinant human TGF- b1 R&D systems Cat# 240-B-010 Recombinant human TPO Peprotech Cat# 300-18 Recombinant human FLT3L Peprotech Cat# 300-19 Recombinant human IL-4 Peprotech Cat# 200-04 Recombinant human M-CSF Peprotech Cat# 300-25 Recombinant murine GM-CSF Peprotech Cat# 315-03 GlutaMAX ThermoFisher Cat# 35050038 Pennicilin/Streptomycin PAA Cat# P11-010 (Continued on next page) Cell Reports 35, 109049, April 27, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Retronectin Takara Cat# T100B Lymphoprep Axis Shield Cat# 1114547 RNase-Free DNase Set QIAGEN Cat#79254 Red Blood Cell Lysing Buffer Hybri-Max Sigma-Aldrich Cat# R7757 DNase I Stem Cell Cat# 07900 Liberase Roche Cat# 5401119001 Hoechst 33342 ThermoFisher Cat# H3570 Imiquimod 5% cream Perrigo N/A Zombie NIR Fixable Viability Kit Biolegend Cat# 423105 Critical commercial assays EasySep human CD34 positive selection kit StemCells Cat# 18056 CD14 MicroBeads, human Miltenyi Cat# 130-050-201 TaqMan MicroRNA Assay hsa-miR-424-5p ThermoFisher Cat# 4427975 (Assay ID: 000604) TaqMan MicroRNA Assay hsa-miR-424-3p ThermoFisher Cat# 4427975 (Assay ID: 002309) TaqMan MicroRNA Assay hsa-miR-503-5p ThermoFisher Cat# 4427975 (Assay ID: 001048) TaqMan MicroRNA Assay U6 snRNA ThermoFisher Cat# 4427975 (Assay ID: 001973) miRNeasy Mini kit QIAGEN Cat# 217004 TaqMan microRNA reverse transcription kit ThermoFisher Cat# 4366596 KAPA RNA HyperPrep Kit with RiboErase (HMR) Roche N/A Deposited data Raw and analyzed data This paper GEO: GSE169717 Experimental models: Cell lines HEK293T Forster et al., 2014 N/A Experimental models: Organisms/strains miR-424(322)/503 knockout mice (Llobet-Navas et al., 2014a) N/A Software and algorithms FlowJo TreeStar https://www.flowjo.com ImageJ NIH https://imagej.nih.gov/ij/ FACS DIVA BD Biosciences N/A Prism 6 GraphPad https://www.graphpad.com TargetScan 7.2 Lewis et al., 2005 http://www.targetscan.org/vert_72/ GSEA software Subramanian et al., 2005 http://www.gsea-msigdb.org/gsea/ index.jsp Salmon Patro et al., 2017 https://bioinformaticshome.com/tools/ rna-seq/descriptions/Salmon.html ..

    Selection:

    Article Title: The miR-424(322)/503 gene cluster regulates pro- versus anti-inflammatory skin DC subset differentiation by modulating TGF-β signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies (clone) Anti-human CD1a (HI149) BD Biosciences Cat# 563938 Anti-human CD83 (HB15e) BD Biosciences Cat# 551073 Anti-human CD80 (L307.4) BD Biosciences Cat# 56113 Anti-human CD86 (FUN-1) BD Biosciences Cat# 561128 Anti-human CD11b (ICRF44) Biolegend Cat# 301306 Anti-human CD207 (DCGM4) Beckman Coulter Cat# IM3577 Anti-human CD209 (eB-h209) eBioscience Cat# 12-2099-42 Anti-human/mouse CD207(929F3.01) Acris Antibodies Cat# DDX0362 Anti-mouse MHC II/ I-A/I-E (M5/114.15.2) Biolegend Cat# 107607 Anti-mouse CD103 (2E7) eBioscience Cat# 50-150-43 Anti-human/mouse CD207 (4c7) Biolegend Cat# 144205 Anti-human/mouse CD11b (M1/70) Biolegend Cat# 101207 Anti-mouse MHC II/ I-A/I-E (M5/114.15.2) Biolegend Cat# 107619 Anti-mouse Ly-6C (HK 1.4) Biolegend Cat# 128017 Anti-mouse CD86 (GL-1) Biolegend Cat# 105013 Anti-mouse CD11c (418) Biolegend Cat# 117325 Anti-mouse CD45 (30-F11) Biolegend Cat# 103108 Anti-mouse EpCAM (G8.8) Biolegend Cat# 118227 Anti-mouse CD64 (290322) R&D Cat# FAB20741c Anti-mouse MerTK (108928) R&D Cat# FAB5912N Anti-mouse CD117/c-kit (2B8) Biolegend Cat# 105815 Anti-mouse CD135 (A2F10) Biolegend Cat# 135309 Anti-mouse CD115/CSF-1R (AFS98) Biolegend Cat# 135531 Anti-mouse CD172a/SIRPa (P84) Biolegend Cat# 144011 Anti-mouse CD16/32 (93) Biolegend Cat# 101301 Bacterial and virus strains MiR Zip-424 anti-miR-424 microRNA construct System Biosciences (SBI) Cat# MZIP424-PA-1 MiR Zip Scramble Hairpin control System Biosciences (SBI) Cat# MZIP000-PA-1 TLR4 agonist - LPS-EK Ultrapure InvivoGen Cat# tlrl-peklps PGN from Staphylococcus aureus Sigma Aldrich Cat# 77140 Chemicals, peptides, and recombinant proteins Recombinant human GM-CSF Peprotech Cat# 300-03 Recombinant human TNF-a Peprotech Cat# 300-01A Recombinant human SCF Peprotech Cat# 300-07 Recombinant human TGF- b1 R&D systems Cat# 240-B-010 Recombinant human TPO Peprotech Cat# 300-18 Recombinant human FLT3L Peprotech Cat# 300-19 Recombinant human IL-4 Peprotech Cat# 200-04 Recombinant human M-CSF Peprotech Cat# 300-25 Recombinant murine GM-CSF Peprotech Cat# 315-03 GlutaMAX ThermoFisher Cat# 35050038 Pennicilin/Streptomycin PAA Cat# P11-010 (Continued on next page) Cell Reports 35, 109049, April 27, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Retronectin Takara Cat# T100B Lymphoprep Axis Shield Cat# 1114547 RNase-Free DNase Set QIAGEN Cat#79254 Red Blood Cell Lysing Buffer Hybri-Max Sigma-Aldrich Cat# R7757 DNase I Stem Cell Cat# 07900 Liberase Roche Cat# 5401119001 Hoechst 33342 ThermoFisher Cat# H3570 Imiquimod 5% cream Perrigo N/A Zombie NIR Fixable Viability Kit Biolegend Cat# 423105 Critical commercial assays EasySep human CD34 positive selection kit StemCells Cat# 18056 CD14 MicroBeads, human Miltenyi Cat# 130-050-201 TaqMan MicroRNA Assay hsa-miR-424-5p ThermoFisher Cat# 4427975 (Assay ID: 000604) TaqMan MicroRNA Assay hsa-miR-424-3p ThermoFisher Cat# 4427975 (Assay ID: 002309) TaqMan MicroRNA Assay hsa-miR-503-5p ThermoFisher Cat# 4427975 (Assay ID: 001048) TaqMan MicroRNA Assay U6 snRNA ThermoFisher Cat# 4427975 (Assay ID: 001973) miRNeasy Mini kit QIAGEN Cat# 217004 TaqMan microRNA reverse transcription kit ThermoFisher Cat# 4366596 KAPA RNA HyperPrep Kit with RiboErase (HMR) Roche N/A Deposited data Raw and analyzed data This paper GEO: GSE169717 Experimental models: Cell lines HEK293T Forster et al., 2014 N/A Experimental models: Organisms/strains miR-424(322)/503 knockout mice (Llobet-Navas et al., 2014a) N/A Software and algorithms FlowJo TreeStar https://www.flowjo.com ImageJ NIH https://imagej.nih.gov/ij/ FACS DIVA BD Biosciences N/A Prism 6 GraphPad https://www.graphpad.com TargetScan 7.2 Lewis et al., 2005 http://www.targetscan.org/vert_72/ GSEA software Subramanian et al., 2005 http://www.gsea-msigdb.org/gsea/ index.jsp Salmon Patro et al., 2017 https://bioinformaticshome.com/tools/ rna-seq/descriptions/Salmon.html ..

    TaqMan microRNA Assay:

    Article Title: The miR-424(322)/503 gene cluster regulates pro- versus anti-inflammatory skin DC subset differentiation by modulating TGF-β signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies (clone) Anti-human CD1a (HI149) BD Biosciences Cat# 563938 Anti-human CD83 (HB15e) BD Biosciences Cat# 551073 Anti-human CD80 (L307.4) BD Biosciences Cat# 56113 Anti-human CD86 (FUN-1) BD Biosciences Cat# 561128 Anti-human CD11b (ICRF44) Biolegend Cat# 301306 Anti-human CD207 (DCGM4) Beckman Coulter Cat# IM3577 Anti-human CD209 (eB-h209) eBioscience Cat# 12-2099-42 Anti-human/mouse CD207(929F3.01) Acris Antibodies Cat# DDX0362 Anti-mouse MHC II/ I-A/I-E (M5/114.15.2) Biolegend Cat# 107607 Anti-mouse CD103 (2E7) eBioscience Cat# 50-150-43 Anti-human/mouse CD207 (4c7) Biolegend Cat# 144205 Anti-human/mouse CD11b (M1/70) Biolegend Cat# 101207 Anti-mouse MHC II/ I-A/I-E (M5/114.15.2) Biolegend Cat# 107619 Anti-mouse Ly-6C (HK 1.4) Biolegend Cat# 128017 Anti-mouse CD86 (GL-1) Biolegend Cat# 105013 Anti-mouse CD11c (418) Biolegend Cat# 117325 Anti-mouse CD45 (30-F11) Biolegend Cat# 103108 Anti-mouse EpCAM (G8.8) Biolegend Cat# 118227 Anti-mouse CD64 (290322) R&D Cat# FAB20741c Anti-mouse MerTK (108928) R&D Cat# FAB5912N Anti-mouse CD117/c-kit (2B8) Biolegend Cat# 105815 Anti-mouse CD135 (A2F10) Biolegend Cat# 135309 Anti-mouse CD115/CSF-1R (AFS98) Biolegend Cat# 135531 Anti-mouse CD172a/SIRPa (P84) Biolegend Cat# 144011 Anti-mouse CD16/32 (93) Biolegend Cat# 101301 Bacterial and virus strains MiR Zip-424 anti-miR-424 microRNA construct System Biosciences (SBI) Cat# MZIP424-PA-1 MiR Zip Scramble Hairpin control System Biosciences (SBI) Cat# MZIP000-PA-1 TLR4 agonist - LPS-EK Ultrapure InvivoGen Cat# tlrl-peklps PGN from Staphylococcus aureus Sigma Aldrich Cat# 77140 Chemicals, peptides, and recombinant proteins Recombinant human GM-CSF Peprotech Cat# 300-03 Recombinant human TNF-a Peprotech Cat# 300-01A Recombinant human SCF Peprotech Cat# 300-07 Recombinant human TGF- b1 R&D systems Cat# 240-B-010 Recombinant human TPO Peprotech Cat# 300-18 Recombinant human FLT3L Peprotech Cat# 300-19 Recombinant human IL-4 Peprotech Cat# 200-04 Recombinant human M-CSF Peprotech Cat# 300-25 Recombinant murine GM-CSF Peprotech Cat# 315-03 GlutaMAX ThermoFisher Cat# 35050038 Pennicilin/Streptomycin PAA Cat# P11-010 (Continued on next page) Cell Reports 35, 109049, April 27, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Retronectin Takara Cat# T100B Lymphoprep Axis Shield Cat# 1114547 RNase-Free DNase Set QIAGEN Cat#79254 Red Blood Cell Lysing Buffer Hybri-Max Sigma-Aldrich Cat# R7757 DNase I Stem Cell Cat# 07900 Liberase Roche Cat# 5401119001 Hoechst 33342 ThermoFisher Cat# H3570 Imiquimod 5% cream Perrigo N/A Zombie NIR Fixable Viability Kit Biolegend Cat# 423105 Critical commercial assays EasySep human CD34 positive selection kit StemCells Cat# 18056 CD14 MicroBeads, human Miltenyi Cat# 130-050-201 TaqMan MicroRNA Assay hsa-miR-424-5p ThermoFisher Cat# 4427975 (Assay ID: 000604) TaqMan MicroRNA Assay hsa-miR-424-3p ThermoFisher Cat# 4427975 (Assay ID: 002309) TaqMan MicroRNA Assay hsa-miR-503-5p ThermoFisher Cat# 4427975 (Assay ID: 001048) TaqMan MicroRNA Assay U6 snRNA ThermoFisher Cat# 4427975 (Assay ID: 001973) miRNeasy Mini kit QIAGEN Cat# 217004 TaqMan microRNA reverse transcription kit ThermoFisher Cat# 4366596 KAPA RNA HyperPrep Kit with RiboErase (HMR) Roche N/A Deposited data Raw and analyzed data This paper GEO: GSE169717 Experimental models: Cell lines HEK293T Forster et al., 2014 N/A Experimental models: Organisms/strains miR-424(322)/503 knockout mice (Llobet-Navas et al., 2014a) N/A Software and algorithms FlowJo TreeStar https://www.flowjo.com ImageJ NIH https://imagej.nih.gov/ij/ FACS DIVA BD Biosciences N/A Prism 6 GraphPad https://www.graphpad.com TargetScan 7.2 Lewis et al., 2005 http://www.targetscan.org/vert_72/ GSEA software Subramanian et al., 2005 http://www.gsea-msigdb.org/gsea/ index.jsp Salmon Patro et al., 2017 https://bioinformaticshome.com/tools/ rna-seq/descriptions/Salmon.html ..

    Reverse Transcription:

    Article Title: The miR-424(322)/503 gene cluster regulates pro- versus anti-inflammatory skin DC subset differentiation by modulating TGF-β signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies (clone) Anti-human CD1a (HI149) BD Biosciences Cat# 563938 Anti-human CD83 (HB15e) BD Biosciences Cat# 551073 Anti-human CD80 (L307.4) BD Biosciences Cat# 56113 Anti-human CD86 (FUN-1) BD Biosciences Cat# 561128 Anti-human CD11b (ICRF44) Biolegend Cat# 301306 Anti-human CD207 (DCGM4) Beckman Coulter Cat# IM3577 Anti-human CD209 (eB-h209) eBioscience Cat# 12-2099-42 Anti-human/mouse CD207(929F3.01) Acris Antibodies Cat# DDX0362 Anti-mouse MHC II/ I-A/I-E (M5/114.15.2) Biolegend Cat# 107607 Anti-mouse CD103 (2E7) eBioscience Cat# 50-150-43 Anti-human/mouse CD207 (4c7) Biolegend Cat# 144205 Anti-human/mouse CD11b (M1/70) Biolegend Cat# 101207 Anti-mouse MHC II/ I-A/I-E (M5/114.15.2) Biolegend Cat# 107619 Anti-mouse Ly-6C (HK 1.4) Biolegend Cat# 128017 Anti-mouse CD86 (GL-1) Biolegend Cat# 105013 Anti-mouse CD11c (418) Biolegend Cat# 117325 Anti-mouse CD45 (30-F11) Biolegend Cat# 103108 Anti-mouse EpCAM (G8.8) Biolegend Cat# 118227 Anti-mouse CD64 (290322) R&D Cat# FAB20741c Anti-mouse MerTK (108928) R&D Cat# FAB5912N Anti-mouse CD117/c-kit (2B8) Biolegend Cat# 105815 Anti-mouse CD135 (A2F10) Biolegend Cat# 135309 Anti-mouse CD115/CSF-1R (AFS98) Biolegend Cat# 135531 Anti-mouse CD172a/SIRPa (P84) Biolegend Cat# 144011 Anti-mouse CD16/32 (93) Biolegend Cat# 101301 Bacterial and virus strains MiR Zip-424 anti-miR-424 microRNA construct System Biosciences (SBI) Cat# MZIP424-PA-1 MiR Zip Scramble Hairpin control System Biosciences (SBI) Cat# MZIP000-PA-1 TLR4 agonist - LPS-EK Ultrapure InvivoGen Cat# tlrl-peklps PGN from Staphylococcus aureus Sigma Aldrich Cat# 77140 Chemicals, peptides, and recombinant proteins Recombinant human GM-CSF Peprotech Cat# 300-03 Recombinant human TNF-a Peprotech Cat# 300-01A Recombinant human SCF Peprotech Cat# 300-07 Recombinant human TGF- b1 R&D systems Cat# 240-B-010 Recombinant human TPO Peprotech Cat# 300-18 Recombinant human FLT3L Peprotech Cat# 300-19 Recombinant human IL-4 Peprotech Cat# 200-04 Recombinant human M-CSF Peprotech Cat# 300-25 Recombinant murine GM-CSF Peprotech Cat# 315-03 GlutaMAX ThermoFisher Cat# 35050038 Pennicilin/Streptomycin PAA Cat# P11-010 (Continued on next page) Cell Reports 35, 109049, April 27, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Retronectin Takara Cat# T100B Lymphoprep Axis Shield Cat# 1114547 RNase-Free DNase Set QIAGEN Cat#79254 Red Blood Cell Lysing Buffer Hybri-Max Sigma-Aldrich Cat# R7757 DNase I Stem Cell Cat# 07900 Liberase Roche Cat# 5401119001 Hoechst 33342 ThermoFisher Cat# H3570 Imiquimod 5% cream Perrigo N/A Zombie NIR Fixable Viability Kit Biolegend Cat# 423105 Critical commercial assays EasySep human CD34 positive selection kit StemCells Cat# 18056 CD14 MicroBeads, human Miltenyi Cat# 130-050-201 TaqMan MicroRNA Assay hsa-miR-424-5p ThermoFisher Cat# 4427975 (Assay ID: 000604) TaqMan MicroRNA Assay hsa-miR-424-3p ThermoFisher Cat# 4427975 (Assay ID: 002309) TaqMan MicroRNA Assay hsa-miR-503-5p ThermoFisher Cat# 4427975 (Assay ID: 001048) TaqMan MicroRNA Assay U6 snRNA ThermoFisher Cat# 4427975 (Assay ID: 001973) miRNeasy Mini kit QIAGEN Cat# 217004 TaqMan microRNA reverse transcription kit ThermoFisher Cat# 4366596 KAPA RNA HyperPrep Kit with RiboErase (HMR) Roche N/A Deposited data Raw and analyzed data This paper GEO: GSE169717 Experimental models: Cell lines HEK293T Forster et al., 2014 N/A Experimental models: Organisms/strains miR-424(322)/503 knockout mice (Llobet-Navas et al., 2014a) N/A Software and algorithms FlowJo TreeStar https://www.flowjo.com ImageJ NIH https://imagej.nih.gov/ij/ FACS DIVA BD Biosciences N/A Prism 6 GraphPad https://www.graphpad.com TargetScan 7.2 Lewis et al., 2005 http://www.targetscan.org/vert_72/ GSEA software Subramanian et al., 2005 http://www.gsea-msigdb.org/gsea/ index.jsp Salmon Patro et al., 2017 https://bioinformaticshome.com/tools/ rna-seq/descriptions/Salmon.html ..

    Knock-Out:

    Article Title: The miR-424(322)/503 gene cluster regulates pro- versus anti-inflammatory skin DC subset differentiation by modulating TGF-β signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies (clone) Anti-human CD1a (HI149) BD Biosciences Cat# 563938 Anti-human CD83 (HB15e) BD Biosciences Cat# 551073 Anti-human CD80 (L307.4) BD Biosciences Cat# 56113 Anti-human CD86 (FUN-1) BD Biosciences Cat# 561128 Anti-human CD11b (ICRF44) Biolegend Cat# 301306 Anti-human CD207 (DCGM4) Beckman Coulter Cat# IM3577 Anti-human CD209 (eB-h209) eBioscience Cat# 12-2099-42 Anti-human/mouse CD207(929F3.01) Acris Antibodies Cat# DDX0362 Anti-mouse MHC II/ I-A/I-E (M5/114.15.2) Biolegend Cat# 107607 Anti-mouse CD103 (2E7) eBioscience Cat# 50-150-43 Anti-human/mouse CD207 (4c7) Biolegend Cat# 144205 Anti-human/mouse CD11b (M1/70) Biolegend Cat# 101207 Anti-mouse MHC II/ I-A/I-E (M5/114.15.2) Biolegend Cat# 107619 Anti-mouse Ly-6C (HK 1.4) Biolegend Cat# 128017 Anti-mouse CD86 (GL-1) Biolegend Cat# 105013 Anti-mouse CD11c (418) Biolegend Cat# 117325 Anti-mouse CD45 (30-F11) Biolegend Cat# 103108 Anti-mouse EpCAM (G8.8) Biolegend Cat# 118227 Anti-mouse CD64 (290322) R&D Cat# FAB20741c Anti-mouse MerTK (108928) R&D Cat# FAB5912N Anti-mouse CD117/c-kit (2B8) Biolegend Cat# 105815 Anti-mouse CD135 (A2F10) Biolegend Cat# 135309 Anti-mouse CD115/CSF-1R (AFS98) Biolegend Cat# 135531 Anti-mouse CD172a/SIRPa (P84) Biolegend Cat# 144011 Anti-mouse CD16/32 (93) Biolegend Cat# 101301 Bacterial and virus strains MiR Zip-424 anti-miR-424 microRNA construct System Biosciences (SBI) Cat# MZIP424-PA-1 MiR Zip Scramble Hairpin control System Biosciences (SBI) Cat# MZIP000-PA-1 TLR4 agonist - LPS-EK Ultrapure InvivoGen Cat# tlrl-peklps PGN from Staphylococcus aureus Sigma Aldrich Cat# 77140 Chemicals, peptides, and recombinant proteins Recombinant human GM-CSF Peprotech Cat# 300-03 Recombinant human TNF-a Peprotech Cat# 300-01A Recombinant human SCF Peprotech Cat# 300-07 Recombinant human TGF- b1 R&D systems Cat# 240-B-010 Recombinant human TPO Peprotech Cat# 300-18 Recombinant human FLT3L Peprotech Cat# 300-19 Recombinant human IL-4 Peprotech Cat# 200-04 Recombinant human M-CSF Peprotech Cat# 300-25 Recombinant murine GM-CSF Peprotech Cat# 315-03 GlutaMAX ThermoFisher Cat# 35050038 Pennicilin/Streptomycin PAA Cat# P11-010 (Continued on next page) Cell Reports 35, 109049, April 27, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Retronectin Takara Cat# T100B Lymphoprep Axis Shield Cat# 1114547 RNase-Free DNase Set QIAGEN Cat#79254 Red Blood Cell Lysing Buffer Hybri-Max Sigma-Aldrich Cat# R7757 DNase I Stem Cell Cat# 07900 Liberase Roche Cat# 5401119001 Hoechst 33342 ThermoFisher Cat# H3570 Imiquimod 5% cream Perrigo N/A Zombie NIR Fixable Viability Kit Biolegend Cat# 423105 Critical commercial assays EasySep human CD34 positive selection kit StemCells Cat# 18056 CD14 MicroBeads, human Miltenyi Cat# 130-050-201 TaqMan MicroRNA Assay hsa-miR-424-5p ThermoFisher Cat# 4427975 (Assay ID: 000604) TaqMan MicroRNA Assay hsa-miR-424-3p ThermoFisher Cat# 4427975 (Assay ID: 002309) TaqMan MicroRNA Assay hsa-miR-503-5p ThermoFisher Cat# 4427975 (Assay ID: 001048) TaqMan MicroRNA Assay U6 snRNA ThermoFisher Cat# 4427975 (Assay ID: 001973) miRNeasy Mini kit QIAGEN Cat# 217004 TaqMan microRNA reverse transcription kit ThermoFisher Cat# 4366596 KAPA RNA HyperPrep Kit with RiboErase (HMR) Roche N/A Deposited data Raw and analyzed data This paper GEO: GSE169717 Experimental models: Cell lines HEK293T Forster et al., 2014 N/A Experimental models: Organisms/strains miR-424(322)/503 knockout mice (Llobet-Navas et al., 2014a) N/A Software and algorithms FlowJo TreeStar https://www.flowjo.com ImageJ NIH https://imagej.nih.gov/ij/ FACS DIVA BD Biosciences N/A Prism 6 GraphPad https://www.graphpad.com TargetScan 7.2 Lewis et al., 2005 http://www.targetscan.org/vert_72/ GSEA software Subramanian et al., 2005 http://www.gsea-msigdb.org/gsea/ index.jsp Salmon Patro et al., 2017 https://bioinformaticshome.com/tools/ rna-seq/descriptions/Salmon.html ..

    Software:

    Article Title: The miR-424(322)/503 gene cluster regulates pro- versus anti-inflammatory skin DC subset differentiation by modulating TGF-β signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies (clone) Anti-human CD1a (HI149) BD Biosciences Cat# 563938 Anti-human CD83 (HB15e) BD Biosciences Cat# 551073 Anti-human CD80 (L307.4) BD Biosciences Cat# 56113 Anti-human CD86 (FUN-1) BD Biosciences Cat# 561128 Anti-human CD11b (ICRF44) Biolegend Cat# 301306 Anti-human CD207 (DCGM4) Beckman Coulter Cat# IM3577 Anti-human CD209 (eB-h209) eBioscience Cat# 12-2099-42 Anti-human/mouse CD207(929F3.01) Acris Antibodies Cat# DDX0362 Anti-mouse MHC II/ I-A/I-E (M5/114.15.2) Biolegend Cat# 107607 Anti-mouse CD103 (2E7) eBioscience Cat# 50-150-43 Anti-human/mouse CD207 (4c7) Biolegend Cat# 144205 Anti-human/mouse CD11b (M1/70) Biolegend Cat# 101207 Anti-mouse MHC II/ I-A/I-E (M5/114.15.2) Biolegend Cat# 107619 Anti-mouse Ly-6C (HK 1.4) Biolegend Cat# 128017 Anti-mouse CD86 (GL-1) Biolegend Cat# 105013 Anti-mouse CD11c (418) Biolegend Cat# 117325 Anti-mouse CD45 (30-F11) Biolegend Cat# 103108 Anti-mouse EpCAM (G8.8) Biolegend Cat# 118227 Anti-mouse CD64 (290322) R&D Cat# FAB20741c Anti-mouse MerTK (108928) R&D Cat# FAB5912N Anti-mouse CD117/c-kit (2B8) Biolegend Cat# 105815 Anti-mouse CD135 (A2F10) Biolegend Cat# 135309 Anti-mouse CD115/CSF-1R (AFS98) Biolegend Cat# 135531 Anti-mouse CD172a/SIRPa (P84) Biolegend Cat# 144011 Anti-mouse CD16/32 (93) Biolegend Cat# 101301 Bacterial and virus strains MiR Zip-424 anti-miR-424 microRNA construct System Biosciences (SBI) Cat# MZIP424-PA-1 MiR Zip Scramble Hairpin control System Biosciences (SBI) Cat# MZIP000-PA-1 TLR4 agonist - LPS-EK Ultrapure InvivoGen Cat# tlrl-peklps PGN from Staphylococcus aureus Sigma Aldrich Cat# 77140 Chemicals, peptides, and recombinant proteins Recombinant human GM-CSF Peprotech Cat# 300-03 Recombinant human TNF-a Peprotech Cat# 300-01A Recombinant human SCF Peprotech Cat# 300-07 Recombinant human TGF- b1 R&D systems Cat# 240-B-010 Recombinant human TPO Peprotech Cat# 300-18 Recombinant human FLT3L Peprotech Cat# 300-19 Recombinant human IL-4 Peprotech Cat# 200-04 Recombinant human M-CSF Peprotech Cat# 300-25 Recombinant murine GM-CSF Peprotech Cat# 315-03 GlutaMAX ThermoFisher Cat# 35050038 Pennicilin/Streptomycin PAA Cat# P11-010 (Continued on next page) Cell Reports 35, 109049, April 27, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Retronectin Takara Cat# T100B Lymphoprep Axis Shield Cat# 1114547 RNase-Free DNase Set QIAGEN Cat#79254 Red Blood Cell Lysing Buffer Hybri-Max Sigma-Aldrich Cat# R7757 DNase I Stem Cell Cat# 07900 Liberase Roche Cat# 5401119001 Hoechst 33342 ThermoFisher Cat# H3570 Imiquimod 5% cream Perrigo N/A Zombie NIR Fixable Viability Kit Biolegend Cat# 423105 Critical commercial assays EasySep human CD34 positive selection kit StemCells Cat# 18056 CD14 MicroBeads, human Miltenyi Cat# 130-050-201 TaqMan MicroRNA Assay hsa-miR-424-5p ThermoFisher Cat# 4427975 (Assay ID: 000604) TaqMan MicroRNA Assay hsa-miR-424-3p ThermoFisher Cat# 4427975 (Assay ID: 002309) TaqMan MicroRNA Assay hsa-miR-503-5p ThermoFisher Cat# 4427975 (Assay ID: 001048) TaqMan MicroRNA Assay U6 snRNA ThermoFisher Cat# 4427975 (Assay ID: 001973) miRNeasy Mini kit QIAGEN Cat# 217004 TaqMan microRNA reverse transcription kit ThermoFisher Cat# 4366596 KAPA RNA HyperPrep Kit with RiboErase (HMR) Roche N/A Deposited data Raw and analyzed data This paper GEO: GSE169717 Experimental models: Cell lines HEK293T Forster et al., 2014 N/A Experimental models: Organisms/strains miR-424(322)/503 knockout mice (Llobet-Navas et al., 2014a) N/A Software and algorithms FlowJo TreeStar https://www.flowjo.com ImageJ NIH https://imagej.nih.gov/ij/ FACS DIVA BD Biosciences N/A Prism 6 GraphPad https://www.graphpad.com TargetScan 7.2 Lewis et al., 2005 http://www.targetscan.org/vert_72/ GSEA software Subramanian et al., 2005 http://www.gsea-msigdb.org/gsea/ index.jsp Salmon Patro et al., 2017 https://bioinformaticshome.com/tools/ rna-seq/descriptions/Salmon.html ..

    FACS:

    Article Title: The miR-424(322)/503 gene cluster regulates pro- versus anti-inflammatory skin DC subset differentiation by modulating TGF-β signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies (clone) Anti-human CD1a (HI149) BD Biosciences Cat# 563938 Anti-human CD83 (HB15e) BD Biosciences Cat# 551073 Anti-human CD80 (L307.4) BD Biosciences Cat# 56113 Anti-human CD86 (FUN-1) BD Biosciences Cat# 561128 Anti-human CD11b (ICRF44) Biolegend Cat# 301306 Anti-human CD207 (DCGM4) Beckman Coulter Cat# IM3577 Anti-human CD209 (eB-h209) eBioscience Cat# 12-2099-42 Anti-human/mouse CD207(929F3.01) Acris Antibodies Cat# DDX0362 Anti-mouse MHC II/ I-A/I-E (M5/114.15.2) Biolegend Cat# 107607 Anti-mouse CD103 (2E7) eBioscience Cat# 50-150-43 Anti-human/mouse CD207 (4c7) Biolegend Cat# 144205 Anti-human/mouse CD11b (M1/70) Biolegend Cat# 101207 Anti-mouse MHC II/ I-A/I-E (M5/114.15.2) Biolegend Cat# 107619 Anti-mouse Ly-6C (HK 1.4) Biolegend Cat# 128017 Anti-mouse CD86 (GL-1) Biolegend Cat# 105013 Anti-mouse CD11c (418) Biolegend Cat# 117325 Anti-mouse CD45 (30-F11) Biolegend Cat# 103108 Anti-mouse EpCAM (G8.8) Biolegend Cat# 118227 Anti-mouse CD64 (290322) R&D Cat# FAB20741c Anti-mouse MerTK (108928) R&D Cat# FAB5912N Anti-mouse CD117/c-kit (2B8) Biolegend Cat# 105815 Anti-mouse CD135 (A2F10) Biolegend Cat# 135309 Anti-mouse CD115/CSF-1R (AFS98) Biolegend Cat# 135531 Anti-mouse CD172a/SIRPa (P84) Biolegend Cat# 144011 Anti-mouse CD16/32 (93) Biolegend Cat# 101301 Bacterial and virus strains MiR Zip-424 anti-miR-424 microRNA construct System Biosciences (SBI) Cat# MZIP424-PA-1 MiR Zip Scramble Hairpin control System Biosciences (SBI) Cat# MZIP000-PA-1 TLR4 agonist - LPS-EK Ultrapure InvivoGen Cat# tlrl-peklps PGN from Staphylococcus aureus Sigma Aldrich Cat# 77140 Chemicals, peptides, and recombinant proteins Recombinant human GM-CSF Peprotech Cat# 300-03 Recombinant human TNF-a Peprotech Cat# 300-01A Recombinant human SCF Peprotech Cat# 300-07 Recombinant human TGF- b1 R&D systems Cat# 240-B-010 Recombinant human TPO Peprotech Cat# 300-18 Recombinant human FLT3L Peprotech Cat# 300-19 Recombinant human IL-4 Peprotech Cat# 200-04 Recombinant human M-CSF Peprotech Cat# 300-25 Recombinant murine GM-CSF Peprotech Cat# 315-03 GlutaMAX ThermoFisher Cat# 35050038 Pennicilin/Streptomycin PAA Cat# P11-010 (Continued on next page) Cell Reports 35, 109049, April 27, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Retronectin Takara Cat# T100B Lymphoprep Axis Shield Cat# 1114547 RNase-Free DNase Set QIAGEN Cat#79254 Red Blood Cell Lysing Buffer Hybri-Max Sigma-Aldrich Cat# R7757 DNase I Stem Cell Cat# 07900 Liberase Roche Cat# 5401119001 Hoechst 33342 ThermoFisher Cat# H3570 Imiquimod 5% cream Perrigo N/A Zombie NIR Fixable Viability Kit Biolegend Cat# 423105 Critical commercial assays EasySep human CD34 positive selection kit StemCells Cat# 18056 CD14 MicroBeads, human Miltenyi Cat# 130-050-201 TaqMan MicroRNA Assay hsa-miR-424-5p ThermoFisher Cat# 4427975 (Assay ID: 000604) TaqMan MicroRNA Assay hsa-miR-424-3p ThermoFisher Cat# 4427975 (Assay ID: 002309) TaqMan MicroRNA Assay hsa-miR-503-5p ThermoFisher Cat# 4427975 (Assay ID: 001048) TaqMan MicroRNA Assay U6 snRNA ThermoFisher Cat# 4427975 (Assay ID: 001973) miRNeasy Mini kit QIAGEN Cat# 217004 TaqMan microRNA reverse transcription kit ThermoFisher Cat# 4366596 KAPA RNA HyperPrep Kit with RiboErase (HMR) Roche N/A Deposited data Raw and analyzed data This paper GEO: GSE169717 Experimental models: Cell lines HEK293T Forster et al., 2014 N/A Experimental models: Organisms/strains miR-424(322)/503 knockout mice (Llobet-Navas et al., 2014a) N/A Software and algorithms FlowJo TreeStar https://www.flowjo.com ImageJ NIH https://imagej.nih.gov/ij/ FACS DIVA BD Biosciences N/A Prism 6 GraphPad https://www.graphpad.com TargetScan 7.2 Lewis et al., 2005 http://www.targetscan.org/vert_72/ GSEA software Subramanian et al., 2005 http://www.gsea-msigdb.org/gsea/ index.jsp Salmon Patro et al., 2017 https://bioinformaticshome.com/tools/ rna-seq/descriptions/Salmon.html ..



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