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Molecular dynamics simulation video of ZAKα-RACK1 corresponding to Figures 7F–7H and <xref ref-type=S7 K. ZAKα residues 336 to 417 (SAM domain, green) and 309 to 329 (C-terminal part of LZ, blue) and RACK1 (orange) are highlighted. Time frame, 100 ns. " width="250" height="auto" />
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Molecular dynamics simulation video of ZAKα-RACK1 corresponding to Figures 7F–7H and <xref ref-type=S7 K. ZAKα residues 336 to 417 (SAM domain, green) and 309 to 329 (C-terminal part of LZ, blue) and RACK1 (orange) are highlighted. Time frame, 100 ns. " width="250" height="auto" />
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Molecular dynamics simulation video of ZAKα-RACK1 corresponding to Figures 7F–7H and <xref ref-type=S7 K. ZAKα residues 336 to 417 (SAM domain, green) and 309 to 329 (C-terminal part of LZ, blue) and RACK1 (orange) are highlighted. Time frame, 100 ns. " width="250" height="auto" />
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Molecular dynamics simulation video of ZAKα-RACK1 corresponding to Figures 7F–7H and <xref ref-type=S7 K. ZAKα residues 336 to 417 (SAM domain, green) and 309 to 329 (C-terminal part of LZ, blue) and RACK1 (orange) are highlighted. Time frame, 100 ns. " width="250" height="auto" />
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Molecular dynamics simulation video of ZAKα-RACK1 corresponding to Figures 7F–7H and <xref ref-type=S7 K. ZAKα residues 336 to 417 (SAM domain, green) and 309 to 329 (C-terminal part of LZ, blue) and RACK1 (orange) are highlighted. Time frame, 100 ns. " width="100%" height="100%">

Journal: Molecular Cell

Article Title: Mechanism of RACK1-dependent ZAKα activation at stalled and collided ribosomes

doi: 10.1016/j.molcel.2026.04.034

Figure Lengend Snippet: Molecular dynamics simulation video of ZAKα-RACK1 corresponding to Figures 7F–7H and S7 K. ZAKα residues 336 to 417 (SAM domain, green) and 309 to 329 (C-terminal part of LZ, blue) and RACK1 (orange) are highlighted. Time frame, 100 ns.

Article Snippet: Antibodies used: Rabbit polyclonal anti-ZAKα (Bethyl, Cat#A301-993A; RRID: AB_1576612 ), Mouse monoclonal anti-phospho-p38 (Cell Signaling, Cat#9216; RRID: AB_331296 ) Rabbit monoclonal anti-phospho-p38 (Cell Signaling, Cat#4511S; RRID: AB_2139682 ), Rabbit polyclonal antibody anti-p38 (Cell Signaling, Cat#9212; RRID: AB_330713 ), Mouse monoclonal anti-phospho-SAPK/JNK (Cell Signaling, Cat#9255; RRID: AB_2307321 ), Rabbit monoclonal anti-phospho-SAPK/JNK (Cell Signaling, Cat#4668, RRID: AB_823588 ), Rabbit monoclonal anti-SAPK/JNK (Cell Signaling, Cat#9258; RRID: AB_2141027 ), Rabbit polyclonal anti-ZAK (Proteintech, Cat#14945-1-AP; RRID: AB_1064269 ), Mouse monoclonal anti-p150 (BD biosciences, Cat#610473; RRID: AB_397845 ), Mouse monoclonal anti-α-Tubulin (Merck, Cat#T9026; RRID: AB_477593 ), Mouse monoclonal anti-HA-tag (Santa Cruz Biotechnology, Cat#sc-7392 HRP; RRID: AB_2894930 ), Mouse monoclonal anti-Puromycin (Millipore, Cat#MABE343; RRID: AB_2566826 ), Rabbit polyclonal anti-RACK1 (Bethyl, Cat#A302-545A; RRID: AB_1999012 ), Rabbit monoclonal anti-RPS10 (Abcam # ab151550; RRID: AB_2714147 ), Rabbit polyclonal anti-GST (Sigma, Cat#G7781; RRID: AB_259965 ), Rabbit polyclonal anti-GCN1 (Thermo Fisher, A301-843A; RRID: AB_1264319 ), Rabbit polyclonal anti-GCN2 (Cell Signaling, Cat#3302S; RRID: AB_2277617 ), Mouse monoclonal anti-GFP (Roche Cat# 11814460001; RRID: AB_390913 ), Mouse monoclonal anti-gamma-tubulin (Sigma, #T5326; RRID: AB_532292 ), Mouse monoclonal anti-RPL19 (Novus Biologicals #H00006143-M01; RRID: AB_509253 ).

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