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control ythdf3 nc lentiviruses  (Genechem)

 
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    Structured Review

    Genechem control ythdf3 nc lentiviruses
    Control Ythdf3 Nc Lentiviruses, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdf3/lentiviral+particles/pm42235938-78-5-12
    Average 86 stars, based on 1 article reviews
    control ythdf3 nc lentiviruses - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    Gene Knockout:

    Article Title: Targeting UXS1-Dependent Glucuronate Detoxification Potentiates Metformin's Anti-Tumor Efficacy in Lung Adenocarcinoma.
    Article Snippet: The survival differences in panel F were evaluated using Advanced Science, 2026 rules of use; w n p d 4 S w s r t U w g 4 T w d g L 4 I l w ( s w T n f p 4 S s s T u g 4 U I w b p c u s A nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202510542 by N at Prov Indonesia, on [14/05/2026] .. All lentiviral particles (including those for gene knockout and UGDH variant overexpression) were customized by GENECHEM (Shanghai, China). ..

    Variant Assay:

    Article Title: Targeting UXS1-Dependent Glucuronate Detoxification Potentiates Metformin's Anti-Tumor Efficacy in Lung Adenocarcinoma.
    Article Snippet: The survival differences in panel F were evaluated using Advanced Science, 2026 rules of use; w n p d 4 S w s r t U w g 4 T w d g L 4 I l w ( s w T n f p 4 S s s T u g 4 U I w b p c u s A nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202510542 by N at Prov Indonesia, on [14/05/2026] .. All lentiviral particles (including those for gene knockout and UGDH variant overexpression) were customized by GENECHEM (Shanghai, China). ..

    Over Expression:

    Article Title: Targeting UXS1-Dependent Glucuronate Detoxification Potentiates Metformin's Anti-Tumor Efficacy in Lung Adenocarcinoma.
    Article Snippet: The survival differences in panel F were evaluated using Advanced Science, 2026 rules of use; w n p d 4 S w s r t U w g 4 T w d g L 4 I l w ( s w T n f p 4 S s s T u g 4 U I w b p c u s A nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202510542 by N at Prov Indonesia, on [14/05/2026] .. All lentiviral particles (including those for gene knockout and UGDH variant overexpression) were customized by GENECHEM (Shanghai, China). ..

    Article Title: YTHDF3 Enhances Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Osteoporosis by Promoting TBX19 Expression.
    Article Snippet: .. Ythdf3 overexpression (Ythdf3- OE) and control (Ythdf3- NC) lentiviruses were constructed by Genechem. ..

    Article Title: Integrative multi-omics analysis identifies key ubiquitination regulators in prostate cancer
    Article Snippet: .. Lentiviral particles expressing TRIM8 (LV-TRIM8, titer: 6.2 × 108 TU/mL), TRIM8-specific short hairpin RNA (LV-TRIM8-RNAi, titer: 7.9 × 108 TU/mL), and their corresponding negative controls (CON335 for overexpression, titer: 9.9 × 108 TU/mL; CON313 for knockdown, titer: 1.0 × 109 TU/mL) were purchased from GeneChem (Shanghai, China). .. Lentiviral particles expressing TRIM8 (LV-TRIM8, titer: 6.2 × 108 TU/mL), TRIM8-specific short hairpin RNA (LV-TRIM8-RNAi, titer: 7.9 × 108 TU/mL), and their corresponding negative controls (CON335 for overexpression, titer: 9.9 × 108 TU/mL; CON313 for knockdown, titer: 1.0 × 109 TU/mL) were purchased from GeneChem (Shanghai, China).

    shRNA:

    Article Title: Rhubarb attenuates intracerebral hemorrhage injury by upregulating Snord17 to inhibit PANoptosis.
    Article Snippet: Background: Intracerebral hemorrhage (ICH) is the most severe subtype of stroke and currently lacks effective therapies.. PANoptosis, a novel programmed cell death mode, plays an important role in ICH.. Rhubarb has shown neuroprotective potential in ICH, but its mechanisms remain unclear.

    Article Title: Integrative multi-omics analysis identifies key ubiquitination regulators in prostate cancer
    Article Snippet: .. Lentiviral particles expressing TRIM8 (LV-TRIM8, titer: 6.2 × 108 TU/mL), TRIM8-specific short hairpin RNA (LV-TRIM8-RNAi, titer: 7.9 × 108 TU/mL), and their corresponding negative controls (CON335 for overexpression, titer: 9.9 × 108 TU/mL; CON313 for knockdown, titer: 1.0 × 109 TU/mL) were purchased from GeneChem (Shanghai, China). .. Lentiviral particles expressing TRIM8 (LV-TRIM8, titer: 6.2 × 108 TU/mL), TRIM8-specific short hairpin RNA (LV-TRIM8-RNAi, titer: 7.9 × 108 TU/mL), and their corresponding negative controls (CON335 for overexpression, titer: 9.9 × 108 TU/mL; CON313 for knockdown, titer: 1.0 × 109 TU/mL) were purchased from GeneChem (Shanghai, China).

    Transfection:

    Article Title: Identification of PRRG1 as a possible molecular target of pancreatic cancer.
    Article Snippet: AR TI CL E IN P RE SS The lentiviral particles encoding PRRG1 (PRRG1 for human) cDNA (“OE-PRRG1”) and the empty vector (“Vector”), provided by Genechem (Shanghai, China), were transfected into CFPAC-1 and PATU-8988T cells. .. The lentiviral particles encoding Prrg1 (Prrg1 for mouse) cDNA (“OE-Prrg1”) and the empty vector (“Vector”), provided by Genechem (Shanghai, China), were transfected into PANC02 cells. ..

    Article Title: Identification of PRRG1 as a possible molecular target of pancreatic cancer.
    Article Snippet: .. AR TI CL E IN P RE SS The lentiviral particles encoding PRRG1 (PRRG1 for human) cDNA (“OE-PRRG1”) and the empty vector (“Vector”), provided by Genechem (Shanghai, China), were transfected into CFPAC-1 and PATU-8988T cells. .. The lentiviral particles encoding Prrg1 (Prrg1 for mouse) cDNA (“OE-Prrg1”) and the empty vector (“Vector”), provided by Genechem (Shanghai, China), were transfected into PANC02 cells.

    Plasmid Preparation:

    Article Title: Chemokine-Like Receptor 1 Knockdown Suppresses Oral Squamous Cell Carcinoma Progression by Reducing Oxidative Phosphorylation
    Article Snippet: Short hairpin RNA (shRNA) lentiviral vectors targeting human CMKLR1 were purchased from OBiO Technology (Shanghai, China). .. Lentiviral particles encoding full-length human CMKLR1 cDNA (vector name: GV358, OE-CMKLR1) were purchased from GeneChem Technology (Shanghai, China). ..

    Control:

    Article Title: YTHDF3 Enhances Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Osteoporosis by Promoting TBX19 Expression.
    Article Snippet: .. Ythdf3 overexpression (Ythdf3- OE) and control (Ythdf3- NC) lentiviruses were constructed by Genechem. ..

    Construct:

    Article Title: YTHDF3 Enhances Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Osteoporosis by Promoting TBX19 Expression.
    Article Snippet: .. Ythdf3 overexpression (Ythdf3- OE) and control (Ythdf3- NC) lentiviruses were constructed by Genechem. ..

    Article Title: PEBP4 alleviates muscle wasting in lung cancer cachexia via KEAP1-NRF2-mediated redox homeostasis.
    Article Snippet: All siRNAs were synthesized by JTSBIO Co., Ltd., Wuhan, China. .. Lentiviral particles encoding PEBP4 (GeneChem, Shanghai, China) or CRISPR/Cas9 constructs targeting KEAP1 (Focus Biology, Shanghai, China), with the sgRNA sequence 5′-GGACTTTCGTAGCCCCCATG-3′, were used to infect C2C12 cells at a multiplicity of infection (MOI) of 30, according to the manufacturers’ instructions. .. Total RNA was isolated from C2C12 myoblasts and skeletal muscle tissues using the MagZol Reagent (Magen, R4801), following the manufacturer’s protocol. cDNA was synthesized from 1 μg of total RNA using the HiScript II Q RT SuperMix (Vazyme, R223-01).

    CRISPR:

    Article Title: PEBP4 alleviates muscle wasting in lung cancer cachexia via KEAP1-NRF2-mediated redox homeostasis.
    Article Snippet: All siRNAs were synthesized by JTSBIO Co., Ltd., Wuhan, China. .. Lentiviral particles encoding PEBP4 (GeneChem, Shanghai, China) or CRISPR/Cas9 constructs targeting KEAP1 (Focus Biology, Shanghai, China), with the sgRNA sequence 5′-GGACTTTCGTAGCCCCCATG-3′, were used to infect C2C12 cells at a multiplicity of infection (MOI) of 30, according to the manufacturers’ instructions. .. Total RNA was isolated from C2C12 myoblasts and skeletal muscle tissues using the MagZol Reagent (Magen, R4801), following the manufacturer’s protocol. cDNA was synthesized from 1 μg of total RNA using the HiScript II Q RT SuperMix (Vazyme, R223-01).

    Sequencing:

    Article Title: PEBP4 alleviates muscle wasting in lung cancer cachexia via KEAP1-NRF2-mediated redox homeostasis.
    Article Snippet: All siRNAs were synthesized by JTSBIO Co., Ltd., Wuhan, China. .. Lentiviral particles encoding PEBP4 (GeneChem, Shanghai, China) or CRISPR/Cas9 constructs targeting KEAP1 (Focus Biology, Shanghai, China), with the sgRNA sequence 5′-GGACTTTCGTAGCCCCCATG-3′, were used to infect C2C12 cells at a multiplicity of infection (MOI) of 30, according to the manufacturers’ instructions. .. Total RNA was isolated from C2C12 myoblasts and skeletal muscle tissues using the MagZol Reagent (Magen, R4801), following the manufacturer’s protocol. cDNA was synthesized from 1 μg of total RNA using the HiScript II Q RT SuperMix (Vazyme, R223-01).

    Infection:

    Article Title: PEBP4 alleviates muscle wasting in lung cancer cachexia via KEAP1-NRF2-mediated redox homeostasis.
    Article Snippet: All siRNAs were synthesized by JTSBIO Co., Ltd., Wuhan, China. .. Lentiviral particles encoding PEBP4 (GeneChem, Shanghai, China) or CRISPR/Cas9 constructs targeting KEAP1 (Focus Biology, Shanghai, China), with the sgRNA sequence 5′-GGACTTTCGTAGCCCCCATG-3′, were used to infect C2C12 cells at a multiplicity of infection (MOI) of 30, according to the manufacturers’ instructions. .. Total RNA was isolated from C2C12 myoblasts and skeletal muscle tissues using the MagZol Reagent (Magen, R4801), following the manufacturer’s protocol. cDNA was synthesized from 1 μg of total RNA using the HiScript II Q RT SuperMix (Vazyme, R223-01).

    Expressing:

    Article Title: Integrative multi-omics analysis identifies key ubiquitination regulators in prostate cancer
    Article Snippet: .. Lentiviral particles expressing TRIM8 (LV-TRIM8, titer: 6.2 × 108 TU/mL), TRIM8-specific short hairpin RNA (LV-TRIM8-RNAi, titer: 7.9 × 108 TU/mL), and their corresponding negative controls (CON335 for overexpression, titer: 9.9 × 108 TU/mL; CON313 for knockdown, titer: 1.0 × 109 TU/mL) were purchased from GeneChem (Shanghai, China). .. Lentiviral particles expressing TRIM8 (LV-TRIM8, titer: 6.2 × 108 TU/mL), TRIM8-specific short hairpin RNA (LV-TRIM8-RNAi, titer: 7.9 × 108 TU/mL), and their corresponding negative controls (CON335 for overexpression, titer: 9.9 × 108 TU/mL; CON313 for knockdown, titer: 1.0 × 109 TU/mL) were purchased from GeneChem (Shanghai, China).

    Knockdown:

    Article Title: Integrative multi-omics analysis identifies key ubiquitination regulators in prostate cancer
    Article Snippet: .. Lentiviral particles expressing TRIM8 (LV-TRIM8, titer: 6.2 × 108 TU/mL), TRIM8-specific short hairpin RNA (LV-TRIM8-RNAi, titer: 7.9 × 108 TU/mL), and their corresponding negative controls (CON335 for overexpression, titer: 9.9 × 108 TU/mL; CON313 for knockdown, titer: 1.0 × 109 TU/mL) were purchased from GeneChem (Shanghai, China). .. Lentiviral particles expressing TRIM8 (LV-TRIM8, titer: 6.2 × 108 TU/mL), TRIM8-specific short hairpin RNA (LV-TRIM8-RNAi, titer: 7.9 × 108 TU/mL), and their corresponding negative controls (CON335 for overexpression, titer: 9.9 × 108 TU/mL; CON313 for knockdown, titer: 1.0 × 109 TU/mL) were purchased from GeneChem (Shanghai, China).



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    YTHDF3 is overexpressed in breast cancer tissues and cells. (A) YTHDF3 expression in breast cancer tissues and adjacent normal tissues in TCGA database; (B) YTHDF3 expression in different types of breast cancer in TCGA database; (C) Kaplan–Meier curves of OS in the TCGA dataset of all breast cancer patients with high and low YTHDF3 expression; (D) YTHDF3 expression in paired breast cancer tissues and adjacent normal tissues; (E) YTHDF3 expression of breast cancer and para‐cancerous tissues was evaluated by immunohistochemistry, ×100; (F) YTHDF3 protein expression in normal human breast cell line MCF‐10A and breast cancer cell lines MCF‐7, MDA‐MB‐231, SK‐BR‐3, ZR‐75‐1 and MDA‐MB‐453; (G) Relative YTHDF3 mRNA expression in normal human breast cell line MCF‐10A and breast cancer cell lines MCF‐7, MDA‐MB‐231, SK‐BR‐3, ZR‐75‐1 and MDA‐MB‐453.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR – HIF1α – LHDA Axis

    doi: 10.1111/jcmm.71105

    Figure Lengend Snippet: YTHDF3 is overexpressed in breast cancer tissues and cells. (A) YTHDF3 expression in breast cancer tissues and adjacent normal tissues in TCGA database; (B) YTHDF3 expression in different types of breast cancer in TCGA database; (C) Kaplan–Meier curves of OS in the TCGA dataset of all breast cancer patients with high and low YTHDF3 expression; (D) YTHDF3 expression in paired breast cancer tissues and adjacent normal tissues; (E) YTHDF3 expression of breast cancer and para‐cancerous tissues was evaluated by immunohistochemistry, ×100; (F) YTHDF3 protein expression in normal human breast cell line MCF‐10A and breast cancer cell lines MCF‐7, MDA‐MB‐231, SK‐BR‐3, ZR‐75‐1 and MDA‐MB‐453; (G) Relative YTHDF3 mRNA expression in normal human breast cell line MCF‐10A and breast cancer cell lines MCF‐7, MDA‐MB‐231, SK‐BR‐3, ZR‐75‐1 and MDA‐MB‐453.

    Article Snippet: Primary antibodies used in the present study were listed as follows: GAPDH (ab313650, Abcam), HIF1 alpha (ab1, Abcam), mTOR (BM4182, Boster), p‐mTOR (BM4840, Boster), RICTOR (A03195‐1, Boster), RAPTOR (BM4438, Boster), AKT (BM4390, Boster), p‐AKT (BM4744, Boster), S6K (BM4240, Boster), p‐S6K (BM4141, Boster), LDHA (A1146, ABclonal), YTHDF3 (A8395, ABclonal), cyclin D1 (A11022, ABclonal), CDK4 (A23522, ABclonal), vimentin ( PAB48967 , Bioswamp), MMP9 (FNab05247, FineTest), LDHB (A5131, ABclonal), PKM1/2 (A27199PM, ABclonal), PFKL (A23687, ABclonal), snail (13099–1‐AP, Proteintech), E‐cadherin (PB9561, Boster), N‐cadherin (A01577‐3, Boster).

    Techniques: Expressing, Immunohistochemistry

    Silence of YTHDF3 restrain cell proliferation and migration of breast cancer cells and arrested the cell cycle. (A) Relative expression of YTHDF3 mRNA and protein after transfection of shNC and shYTHDF3 in MDA‐MB‐231 and MCF‐7 cells; (B) the growth ability of MDA‐MB‐231 and MCF‐7 cells transfected with shNC and shYTHDF3 was detected by CCK‐8 assay; (C) colony formation assay was used to detect the proliferation ability of MDA‐MB‐231 and MCF‐7 cells in shNC and shYTHDF3 groups; (D) the cell cycle distribution of MDA‐MB‐231 and MCF‐7 cells in shNC and shYTHDF3 groups was detected by flow cytometry; (E) expression of cyclin D1 and CDK4 in shNC and shYTHDF3 in MDA‐MB‐231 and MCF‐7 cells; (F, G) transwell invasion assay was used to detect the migration ability of breast cancer cells in the shNC group and the shYTHDF3 group in MCF‐7 and MDA‐MB‐231 cells; (H) the expression of vimentin, MMP9, E‐cadherin, N‐cadherin and snail in shNC and shYTHDF3 in MDA‐MB‐231 and MCF‐7 cells.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR – HIF1α – LHDA Axis

    doi: 10.1111/jcmm.71105

    Figure Lengend Snippet: Silence of YTHDF3 restrain cell proliferation and migration of breast cancer cells and arrested the cell cycle. (A) Relative expression of YTHDF3 mRNA and protein after transfection of shNC and shYTHDF3 in MDA‐MB‐231 and MCF‐7 cells; (B) the growth ability of MDA‐MB‐231 and MCF‐7 cells transfected with shNC and shYTHDF3 was detected by CCK‐8 assay; (C) colony formation assay was used to detect the proliferation ability of MDA‐MB‐231 and MCF‐7 cells in shNC and shYTHDF3 groups; (D) the cell cycle distribution of MDA‐MB‐231 and MCF‐7 cells in shNC and shYTHDF3 groups was detected by flow cytometry; (E) expression of cyclin D1 and CDK4 in shNC and shYTHDF3 in MDA‐MB‐231 and MCF‐7 cells; (F, G) transwell invasion assay was used to detect the migration ability of breast cancer cells in the shNC group and the shYTHDF3 group in MCF‐7 and MDA‐MB‐231 cells; (H) the expression of vimentin, MMP9, E‐cadherin, N‐cadherin and snail in shNC and shYTHDF3 in MDA‐MB‐231 and MCF‐7 cells.

    Article Snippet: Primary antibodies used in the present study were listed as follows: GAPDH (ab313650, Abcam), HIF1 alpha (ab1, Abcam), mTOR (BM4182, Boster), p‐mTOR (BM4840, Boster), RICTOR (A03195‐1, Boster), RAPTOR (BM4438, Boster), AKT (BM4390, Boster), p‐AKT (BM4744, Boster), S6K (BM4240, Boster), p‐S6K (BM4141, Boster), LDHA (A1146, ABclonal), YTHDF3 (A8395, ABclonal), cyclin D1 (A11022, ABclonal), CDK4 (A23522, ABclonal), vimentin ( PAB48967 , Bioswamp), MMP9 (FNab05247, FineTest), LDHB (A5131, ABclonal), PKM1/2 (A27199PM, ABclonal), PFKL (A23687, ABclonal), snail (13099–1‐AP, Proteintech), E‐cadherin (PB9561, Boster), N‐cadherin (A01577‐3, Boster).

    Techniques: Migration, Expressing, Transfection, CCK-8 Assay, Colony Assay, Flow Cytometry, Transwell Invasion Assay

    Silencing YTHDF3 caused a decrease in lactate levels and affected glycolysis. (A) Lactate levels in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (B) extracellular acidification rate (ECAR) in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells measured by a seahorse instrument; (C) oxygen consumption rate (OCR) in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells measured by a seahorse instrument; (D) relative expression levels of hexokinase‐1 (HK1), hexokinase‐2 (HK2), PFKL, PKM, LDHA and LDHB in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (E) protein expression levels of PFKL, PKM, LDHA and LDHB in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; and (F) the correlation between YTHDF3 and PFKL, PKM, LDHA and LDHB was predicted using the GEPIA website.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR – HIF1α – LHDA Axis

    doi: 10.1111/jcmm.71105

    Figure Lengend Snippet: Silencing YTHDF3 caused a decrease in lactate levels and affected glycolysis. (A) Lactate levels in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (B) extracellular acidification rate (ECAR) in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells measured by a seahorse instrument; (C) oxygen consumption rate (OCR) in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells measured by a seahorse instrument; (D) relative expression levels of hexokinase‐1 (HK1), hexokinase‐2 (HK2), PFKL, PKM, LDHA and LDHB in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (E) protein expression levels of PFKL, PKM, LDHA and LDHB in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; and (F) the correlation between YTHDF3 and PFKL, PKM, LDHA and LDHB was predicted using the GEPIA website.

    Article Snippet: Primary antibodies used in the present study were listed as follows: GAPDH (ab313650, Abcam), HIF1 alpha (ab1, Abcam), mTOR (BM4182, Boster), p‐mTOR (BM4840, Boster), RICTOR (A03195‐1, Boster), RAPTOR (BM4438, Boster), AKT (BM4390, Boster), p‐AKT (BM4744, Boster), S6K (BM4240, Boster), p‐S6K (BM4141, Boster), LDHA (A1146, ABclonal), YTHDF3 (A8395, ABclonal), cyclin D1 (A11022, ABclonal), CDK4 (A23522, ABclonal), vimentin ( PAB48967 , Bioswamp), MMP9 (FNab05247, FineTest), LDHB (A5131, ABclonal), PKM1/2 (A27199PM, ABclonal), PFKL (A23687, ABclonal), snail (13099–1‐AP, Proteintech), E‐cadherin (PB9561, Boster), N‐cadherin (A01577‐3, Boster).

    Techniques: Expressing

    YTHDF3 regulates the glycolysis level of breast cancer through the mTOR–HIF1α–LDHA axis. (A) Relative mRNA expression of HIF1α in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (B) the correlation between YTHDF3 and HIF1α was predicted using the GEPIA website; (C) expression of key proteins in the mTOR–HIF1α–LDHA axis in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (D) after adding activator MHY1445, the growth ability of cancer cells in shNC and shYTHDF3 groups was detected by CCK‐8 assay; (E) the lactate level of cancer cells in shNC and shYTHDF3 groups treated with activator MHY1485; and (F) expression of proteins of cancer cells in shNC and shYTHDF3 groups treated with activator MHY1485.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR – HIF1α – LHDA Axis

    doi: 10.1111/jcmm.71105

    Figure Lengend Snippet: YTHDF3 regulates the glycolysis level of breast cancer through the mTOR–HIF1α–LDHA axis. (A) Relative mRNA expression of HIF1α in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (B) the correlation between YTHDF3 and HIF1α was predicted using the GEPIA website; (C) expression of key proteins in the mTOR–HIF1α–LDHA axis in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (D) after adding activator MHY1445, the growth ability of cancer cells in shNC and shYTHDF3 groups was detected by CCK‐8 assay; (E) the lactate level of cancer cells in shNC and shYTHDF3 groups treated with activator MHY1485; and (F) expression of proteins of cancer cells in shNC and shYTHDF3 groups treated with activator MHY1485.

    Article Snippet: Primary antibodies used in the present study were listed as follows: GAPDH (ab313650, Abcam), HIF1 alpha (ab1, Abcam), mTOR (BM4182, Boster), p‐mTOR (BM4840, Boster), RICTOR (A03195‐1, Boster), RAPTOR (BM4438, Boster), AKT (BM4390, Boster), p‐AKT (BM4744, Boster), S6K (BM4240, Boster), p‐S6K (BM4141, Boster), LDHA (A1146, ABclonal), YTHDF3 (A8395, ABclonal), cyclin D1 (A11022, ABclonal), CDK4 (A23522, ABclonal), vimentin ( PAB48967 , Bioswamp), MMP9 (FNab05247, FineTest), LDHB (A5131, ABclonal), PKM1/2 (A27199PM, ABclonal), PFKL (A23687, ABclonal), snail (13099–1‐AP, Proteintech), E‐cadherin (PB9561, Boster), N‐cadherin (A01577‐3, Boster).

    Techniques: Expressing, CCK-8 Assay

    YTHDF3 regulates breast cancer development through the mTOR–HIF1α–LDHA axis in vivo. (A) Tumour volumes in shNC and shYTHDF3 groups on the indicated days; (B) representative tumour images and (C) tumour weights in shNC and shYTHDF3 groups on day 63; (D) mRNA and (E) protein expression levels of YTHDF3, HIF1α and LDHA in the tumours in shNC and shYTHDF3 groups; (F) Immunohistochemical images of YTHDF3, Ki67, HIF1α and LDHA in tumours in the shNC and shYTHDF3 groups, ×100.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR – HIF1α – LHDA Axis

    doi: 10.1111/jcmm.71105

    Figure Lengend Snippet: YTHDF3 regulates breast cancer development through the mTOR–HIF1α–LDHA axis in vivo. (A) Tumour volumes in shNC and shYTHDF3 groups on the indicated days; (B) representative tumour images and (C) tumour weights in shNC and shYTHDF3 groups on day 63; (D) mRNA and (E) protein expression levels of YTHDF3, HIF1α and LDHA in the tumours in shNC and shYTHDF3 groups; (F) Immunohistochemical images of YTHDF3, Ki67, HIF1α and LDHA in tumours in the shNC and shYTHDF3 groups, ×100.

    Article Snippet: Primary antibodies used in the present study were listed as follows: GAPDH (ab313650, Abcam), HIF1 alpha (ab1, Abcam), mTOR (BM4182, Boster), p‐mTOR (BM4840, Boster), RICTOR (A03195‐1, Boster), RAPTOR (BM4438, Boster), AKT (BM4390, Boster), p‐AKT (BM4744, Boster), S6K (BM4240, Boster), p‐S6K (BM4141, Boster), LDHA (A1146, ABclonal), YTHDF3 (A8395, ABclonal), cyclin D1 (A11022, ABclonal), CDK4 (A23522, ABclonal), vimentin ( PAB48967 , Bioswamp), MMP9 (FNab05247, FineTest), LDHB (A5131, ABclonal), PKM1/2 (A27199PM, ABclonal), PFKL (A23687, ABclonal), snail (13099–1‐AP, Proteintech), E‐cadherin (PB9561, Boster), N‐cadherin (A01577‐3, Boster).

    Techniques: In Vivo, Expressing, Immunohistochemical staining

    YTHDF3 is associated with prognosis in several cancers. (A) Column nomogram was constructed using YTHDF3 expression levels, age, T stage, N stage, M stage and tumour stage data of breast cancer patients; (B–E) survival analysis software package was used to analyse the survival prognosis difference between patients with high and low YTHDF3 expression in BRCA, DLBC, THCA and UVM.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR – HIF1α – LHDA Axis

    doi: 10.1111/jcmm.71105

    Figure Lengend Snippet: YTHDF3 is associated with prognosis in several cancers. (A) Column nomogram was constructed using YTHDF3 expression levels, age, T stage, N stage, M stage and tumour stage data of breast cancer patients; (B–E) survival analysis software package was used to analyse the survival prognosis difference between patients with high and low YTHDF3 expression in BRCA, DLBC, THCA and UVM.

    Article Snippet: Primary antibodies used in the present study were listed as follows: GAPDH (ab313650, Abcam), HIF1 alpha (ab1, Abcam), mTOR (BM4182, Boster), p‐mTOR (BM4840, Boster), RICTOR (A03195‐1, Boster), RAPTOR (BM4438, Boster), AKT (BM4390, Boster), p‐AKT (BM4744, Boster), S6K (BM4240, Boster), p‐S6K (BM4141, Boster), LDHA (A1146, ABclonal), YTHDF3 (A8395, ABclonal), cyclin D1 (A11022, ABclonal), CDK4 (A23522, ABclonal), vimentin ( PAB48967 , Bioswamp), MMP9 (FNab05247, FineTest), LDHB (A5131, ABclonal), PKM1/2 (A27199PM, ABclonal), PFKL (A23687, ABclonal), snail (13099–1‐AP, Proteintech), E‐cadherin (PB9561, Boster), N‐cadherin (A01577‐3, Boster).

    Techniques: Construct, Expressing, Software