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Incyte corporation cdna arrays
Cdna Arrays, supplied by Incyte corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u133a+dna+microarray/cdna+microarray/pmc01140753-169-1-0
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cdna arrays - by Bioz Stars, 2026-09
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Related Articles

Microarray:

Article Title: High-Throughput Transcriptome Profiling in Drug and Biomarker Discovery
Article Snippet: .. They used cDNA microarray tools (Incyte, Inc., MO, U.S.A.) to identify the candidate genes responsible for enhancing memory and evaluate the specific gene expression patterns by real-time PCR in SD rat model of passive avoidance task. ..

Article Title: ASBMR 23nd annual meeting.
Article Snippet: .. In this study, cDNA microarray analysis was conducted by Incyte Genomic Systems on 8,734 gene accessions using mRNA from mouse femurs (free of marrow) of both the congenic and B6 strains to test two hypotheses: 1) microarray analysis will identify candidate genes for bone density; and 2) by comparing the gene expression profile outside of the QTL region between B6 and the congenic mice, we can better characterize the molecular phenotypic mechanisms that contribute to the high bone density QTL. ..

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Article Title: Differentially expressed genes in large granular lymphocyte leukemia
Article Snippet: .. Overexpression of a variety cytotoxic genes was observed in leukemic LGL utilizing cDNA microarray from Incyte Genomics (FIG. 1 and Table 4). .. To verify the identity of these overexpressed genes, clones containing cDNA fragments of the selected genes were obtained from Incyte Genomics and confirmed by sequencing.

Article Title: A review of Omics research in acupuncture: the relevance and future prospects for understanding the nature of meridians and acupoints.
Article Snippet: .. Insulin-like growth factor-1 (IGF-1) was one of the most notable up-regulated J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 597 598 J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 Table 1 (Continued) Ref/Year Acupoints Treatment Subject groups Targeted disease/symptom Tissue sample Technique Verification/ validation Identified candidate gene Implication Ko/2002 ST36 (Zusanli) EA SD rats (n = 7/group): normal/neuropathic pain/EA treatment/ Neuropathic pain RNA from spinal nerves [pooled RNA samples] cDNA microarray (Incyte genomics) Dot-blot Up: opioid receptor, MAP kinase, zinc finger protein, and tyrosine phosphatase related genes Pain and analgesic Pan/2011 MS6 (scalp acu motor areas)/BL10(Tianzhu)/GB20 (Fengchi)/ LI4 (Hegu)/PC6 (Neiguan)/BL40 (Weizhong)/SP6 (Sanyinjiao)/S36 (Zusanli) EA 35 human: drug treatment/EA + drug treatment/normal Acute ischemic stroke Serum protein 2-DE + MS/MS Western blot Up: Gelsolin, C3, down: Serpin G1 Neuroprotection Kim/2010 HT8 (Sobu) Acupuncture SD rats (n = 34): pup+ mother/pup + mother +acupuncture/maternally separated(MS)/MS + acupuncture/ Maternal separation Protein sample from hypothalamic area 2-DE + MALDITOF/TOF MS N.A. ..

Article Title: Ectonucleoside triphosphate diphosphohydrolase 6 expression in testis and testicular cancer and its implication in cisplatin resistance.
Article Snippet: After 48 h, the surviving cells were stained with the alamarBlue assay (treK Diagnostic systems, cleveland, oH, UsA) for 180 min at 37°c. the absorbances of the wells were measured using a plate reader (ArVoTM MX; perkin elmer Inc., Waltham, MA, UsA). cDNA microarray analysis. the procedure of cDnA microarray analysis was performed as described previously (18). .. Briefly, cDNA generation, hybridization, and data collection for cDnA microarray analyses were performed by the Incyte corporation (palo Alto, cA, UsA). ..

Gene Expression:

Article Title: High-Throughput Transcriptome Profiling in Drug and Biomarker Discovery
Article Snippet: .. They used cDNA microarray tools (Incyte, Inc., MO, U.S.A.) to identify the candidate genes responsible for enhancing memory and evaluate the specific gene expression patterns by real-time PCR in SD rat model of passive avoidance task. ..

Article Title: ASBMR 23nd annual meeting.
Article Snippet: .. In this study, cDNA microarray analysis was conducted by Incyte Genomic Systems on 8,734 gene accessions using mRNA from mouse femurs (free of marrow) of both the congenic and B6 strains to test two hypotheses: 1) microarray analysis will identify candidate genes for bone density; and 2) by comparing the gene expression profile outside of the QTL region between B6 and the congenic mice, we can better characterize the molecular phenotypic mechanisms that contribute to the high bone density QTL. ..

Real-time Polymerase Chain Reaction:

Article Title: High-Throughput Transcriptome Profiling in Drug and Biomarker Discovery
Article Snippet: .. They used cDNA microarray tools (Incyte, Inc., MO, U.S.A.) to identify the candidate genes responsible for enhancing memory and evaluate the specific gene expression patterns by real-time PCR in SD rat model of passive avoidance task. ..

Labeling:

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Hybridization:

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Article Title: Ectonucleoside triphosphate diphosphohydrolase 6 expression in testis and testicular cancer and its implication in cisplatin resistance.
Article Snippet: After 48 h, the surviving cells were stained with the alamarBlue assay (treK Diagnostic systems, cleveland, oH, UsA) for 180 min at 37°c. the absorbances of the wells were measured using a plate reader (ArVoTM MX; perkin elmer Inc., Waltham, MA, UsA). cDNA microarray analysis. the procedure of cDnA microarray analysis was performed as described previously (18). .. Briefly, cDNA generation, hybridization, and data collection for cDnA microarray analyses were performed by the Incyte corporation (palo Alto, cA, UsA). ..

Clone Assay:

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Amplification:

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Over Expression:

Article Title: Differentially expressed genes in large granular lymphocyte leukemia
Article Snippet: .. Overexpression of a variety cytotoxic genes was observed in leukemic LGL utilizing cDNA microarray from Incyte Genomics (FIG. 1 and Table 4). .. To verify the identity of these overexpressed genes, clones containing cDNA fragments of the selected genes were obtained from Incyte Genomics and confirmed by sequencing.

other:

Article Title: Three microarray platforms: an analysis of their concordance in profiling gene expression
Article Snippet: Incyte cDNA arrays and Operon long oligonucleotide (70-mer) arrays were printed in house, and Affymetrix 25-mer HG U133A arrays were purchased from the manufacturer.

Article Title: Multifactorial regulation of E-cadherin expression: an integrative study
Article Snippet: We have previously described our processing and normalization of NCI-60 transcript expression data from pin-spotted cDNA arrays (Incyte, Inc., Palo Alto, CA) ( 27 , 28 ), Affymetrix Hu-6800 arrays (Affymetrix, Sunnyvale, CA) ( 29 ), Affymetrix HG-U95 arrays ( 30 ), and Affymetrix HG-U133 ( 30 ).

Biomarker Discovery:

Article Title: A review of Omics research in acupuncture: the relevance and future prospects for understanding the nature of meridians and acupoints.
Article Snippet: .. Insulin-like growth factor-1 (IGF-1) was one of the most notable up-regulated J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 597 598 J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 Table 1 (Continued) Ref/Year Acupoints Treatment Subject groups Targeted disease/symptom Tissue sample Technique Verification/ validation Identified candidate gene Implication Ko/2002 ST36 (Zusanli) EA SD rats (n = 7/group): normal/neuropathic pain/EA treatment/ Neuropathic pain RNA from spinal nerves [pooled RNA samples] cDNA microarray (Incyte genomics) Dot-blot Up: opioid receptor, MAP kinase, zinc finger protein, and tyrosine phosphatase related genes Pain and analgesic Pan/2011 MS6 (scalp acu motor areas)/BL10(Tianzhu)/GB20 (Fengchi)/ LI4 (Hegu)/PC6 (Neiguan)/BL40 (Weizhong)/SP6 (Sanyinjiao)/S36 (Zusanli) EA 35 human: drug treatment/EA + drug treatment/normal Acute ischemic stroke Serum protein 2-DE + MS/MS Western blot Up: Gelsolin, C3, down: Serpin G1 Neuroprotection Kim/2010 HT8 (Sobu) Acupuncture SD rats (n = 34): pup+ mother/pup + mother +acupuncture/maternally separated(MS)/MS + acupuncture/ Maternal separation Protein sample from hypothalamic area 2-DE + MALDITOF/TOF MS N.A. ..

Tandem Mass Spectroscopy:

Article Title: A review of Omics research in acupuncture: the relevance and future prospects for understanding the nature of meridians and acupoints.
Article Snippet: .. Insulin-like growth factor-1 (IGF-1) was one of the most notable up-regulated J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 597 598 J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 Table 1 (Continued) Ref/Year Acupoints Treatment Subject groups Targeted disease/symptom Tissue sample Technique Verification/ validation Identified candidate gene Implication Ko/2002 ST36 (Zusanli) EA SD rats (n = 7/group): normal/neuropathic pain/EA treatment/ Neuropathic pain RNA from spinal nerves [pooled RNA samples] cDNA microarray (Incyte genomics) Dot-blot Up: opioid receptor, MAP kinase, zinc finger protein, and tyrosine phosphatase related genes Pain and analgesic Pan/2011 MS6 (scalp acu motor areas)/BL10(Tianzhu)/GB20 (Fengchi)/ LI4 (Hegu)/PC6 (Neiguan)/BL40 (Weizhong)/SP6 (Sanyinjiao)/S36 (Zusanli) EA 35 human: drug treatment/EA + drug treatment/normal Acute ischemic stroke Serum protein 2-DE + MS/MS Western blot Up: Gelsolin, C3, down: Serpin G1 Neuroprotection Kim/2010 HT8 (Sobu) Acupuncture SD rats (n = 34): pup+ mother/pup + mother +acupuncture/maternally separated(MS)/MS + acupuncture/ Maternal separation Protein sample from hypothalamic area 2-DE + MALDITOF/TOF MS N.A. ..

Western Blot:

Article Title: A review of Omics research in acupuncture: the relevance and future prospects for understanding the nature of meridians and acupoints.
Article Snippet: .. Insulin-like growth factor-1 (IGF-1) was one of the most notable up-regulated J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 597 598 J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 Table 1 (Continued) Ref/Year Acupoints Treatment Subject groups Targeted disease/symptom Tissue sample Technique Verification/ validation Identified candidate gene Implication Ko/2002 ST36 (Zusanli) EA SD rats (n = 7/group): normal/neuropathic pain/EA treatment/ Neuropathic pain RNA from spinal nerves [pooled RNA samples] cDNA microarray (Incyte genomics) Dot-blot Up: opioid receptor, MAP kinase, zinc finger protein, and tyrosine phosphatase related genes Pain and analgesic Pan/2011 MS6 (scalp acu motor areas)/BL10(Tianzhu)/GB20 (Fengchi)/ LI4 (Hegu)/PC6 (Neiguan)/BL40 (Weizhong)/SP6 (Sanyinjiao)/S36 (Zusanli) EA 35 human: drug treatment/EA + drug treatment/normal Acute ischemic stroke Serum protein 2-DE + MS/MS Western blot Up: Gelsolin, C3, down: Serpin G1 Neuroprotection Kim/2010 HT8 (Sobu) Acupuncture SD rats (n = 34): pup+ mother/pup + mother +acupuncture/maternally separated(MS)/MS + acupuncture/ Maternal separation Protein sample from hypothalamic area 2-DE + MALDITOF/TOF MS N.A. ..



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Image Search Results


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Journal: Breast Cancer (Tokyo, Japan)

Article Title: IRSN-23 gene diagnosis enhances breast cancer subtype classification and predicts response to neoadjuvant chemotherapy: new validation analyses

doi: 10.1007/s12282-025-01687-6

Figure Lengend Snippet: Overview of IRSN-23 pCR-prediction without anti-HER2 therapy in breast cancer. A Meta-analysis of all datasets. The gray-shaded areas show the results of the present study. B Pooled analysis of each subtype and treatment. C IRSN-23 immune scores (IS) and pCR rates for each subtype. The solid lines and bands represent loess regression fitting (span = 2), and 95% confidence intervals were implemented using the R function geom_smooth. Abbreviations in the figure are as follows: GPL570 Affymetrix Human Genome U133 Plus 2.0 Array, GPL571 Affymetrix Human Genome U133A 2.0 Array, GPL96 Affymetrix Human Genome U133A Array, GPL6884 Illumina HumanWG-6 v3.0 expression beadchip, GPL1352 Affymetrix Human X3P, GPL6480 Agilent-014850 Whole Human Genome Microarray 4 × 44 K G4112F, A anthracycline, T taxane, Pac paclitaxel, Doc docetaxel, Ixa ixabepilone. Predictors of chemotherapy sensitivity and clinical utility

Article Snippet: Gene expression analysis was performed using Affymetrix U133A Plus 2.0 DNA microarray since the last publication.

Techniques: Expressing, Microarray

Overview of the production and use of expression microarrays. 3′ Expression arrays use synthetically derived oligo probes with design based on mRNA Databases (RefSeq mRNAs, GenBank mRNAs, and ESTs from dbEST) or cDNA derived from bacterial libraries (see ). Sample mRNA can be labeled using two methods (a) Cy3/Cy5 labeling: sample mRNA is reverse transcribed into cDNA and Cy3 is added to one sample and Cy5 to another. Both labeled samples are hybridized to the same microarray. (b) 3′ IVT array: sample mRNA is reverse transcribed to cDNA using oligo(dT) primers, to provide a template for transcription. Using biotin-conjugated nucleotides, the template cDNA is then converted to amplified RNA (aRNA). The biotin-labeled aRNA samples are then fragmented and hybridized onto 3′ expression arrays. A biotin binding fluorescent stain is added to the microarray after hybridization. (c) Affymetrix HuExon 1.0 ST: sample mRNA is reverse transcribed to cDNA using random primers, to provide a template for transcription. The resulting RNA is then reverse transcribed in the presence of dUTPs which are incorporated occasionally into the cDNA sequence instead of dTTP. An enzyme is then used to cleave the cDNA at the site of dUTP incorporation and fragments are terminally labeled before hybridization onto the array. The microarray is then washed and stained after hybridization.

Journal: Journal of Allergy

Article Title: How Can Microarrays Unlock Asthma?

doi: 10.1155/2012/241314

Figure Lengend Snippet: Overview of the production and use of expression microarrays. 3′ Expression arrays use synthetically derived oligo probes with design based on mRNA Databases (RefSeq mRNAs, GenBank mRNAs, and ESTs from dbEST) or cDNA derived from bacterial libraries (see ). Sample mRNA can be labeled using two methods (a) Cy3/Cy5 labeling: sample mRNA is reverse transcribed into cDNA and Cy3 is added to one sample and Cy5 to another. Both labeled samples are hybridized to the same microarray. (b) 3′ IVT array: sample mRNA is reverse transcribed to cDNA using oligo(dT) primers, to provide a template for transcription. Using biotin-conjugated nucleotides, the template cDNA is then converted to amplified RNA (aRNA). The biotin-labeled aRNA samples are then fragmented and hybridized onto 3′ expression arrays. A biotin binding fluorescent stain is added to the microarray after hybridization. (c) Affymetrix HuExon 1.0 ST: sample mRNA is reverse transcribed to cDNA using random primers, to provide a template for transcription. The resulting RNA is then reverse transcribed in the presence of dUTPs which are incorporated occasionally into the cDNA sequence instead of dTTP. An enzyme is then used to cleave the cDNA at the site of dUTP incorporation and fragments are terminally labeled before hybridization onto the array. The microarray is then washed and stained after hybridization.

Article Snippet: 2010 , Transglutaminase 2, a novel regulator of eicosanoid production in asthma revealed by genomewide expression profiling of distinct asthma phenotypes , Affymetrix Human U133A DNA microarrays Human Genome U133 Plus 2.0 GeneChip arrays , GSE13785 , [ ] .

Techniques: Expressing, Derivative Assay, Labeling, Microarray, Amplification, Binding Assay, Staining, Hybridization, Sequencing

The GEO accession number for microarray studies conducted on asthma.

Journal: Journal of Allergy

Article Title: How Can Microarrays Unlock Asthma?

doi: 10.1155/2012/241314

Figure Lengend Snippet: The GEO accession number for microarray studies conducted on asthma.

Article Snippet: 2010 , Transglutaminase 2, a novel regulator of eicosanoid production in asthma revealed by genomewide expression profiling of distinct asthma phenotypes , Affymetrix Human U133A DNA microarrays Human Genome U133 Plus 2.0 GeneChip arrays , GSE13785 , [ ] .

Techniques: Microarray, Expressing, Clone Assay, Infection, Functional Assay, Sequencing, Synthesized