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Harvard Bioscience two-needle array electrodes (450121)
Two Needle Array Electrodes (450121), supplied by Harvard Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-needle+array+electrodes/2+needle+array/pmc05323275-125-0-4
Average 90 stars, based on 1 article reviews
two-needle array electrodes (450121) - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Plasmid Preparation:

Article Title: Chirality and asymmetry increase the potency of candidate ADRM1/RPN13 inhibitors
Article Snippet: .. A patch of CD1 mouse leg was shaved of hair and 10 μg 4Ub-FL plasmid in 20 μL of PBS was injected into the quadriceps femoralis muscle followed immediately by injection of the 2 Needle Array to 5 mm depth encompassing the injection site and square wave electroporation (ElectroSquarePorator 833, BTX-2 Needle array 5mm gap, Harvard apparatus) delivered as eight pulses at 106V for 20 ms with 200 ms intervals. ..

Article Title: Structure-function analyses of candidate small molecule RPN13 inhibitors with antitumor properties
Article Snippet: .. A patch of Balb/c mouse leg was shaved of hair and 10 μg 4Ub-FL plasmid in 20 μL of PBS was injected into the quadriceps femoralis muscle followed immediately by injection of the 2 Needle Array to 5 mm depth encompassing the injection site and square wave electroporation (ElectroSquarePorator 833, BTX-2 Needle array 5mm gap, Harvard apparatus) delivered as eight pulses at 106V for 20 ms with 200 ms intervals. ..

Article Title: Covalent Rpn13-Binding Inhibitors for the Treatment of Ovarian Cancer
Article Snippet: Gene gun particle-mediated DNA delivery was performed using a helium-driven gene gun (Bio-Rad, Hercules, CA), as described previously. .. For electroporation, a patch of the mouse leg was shaved and 10 μg of 4UbFL or FL DNA plasmid in 20 μL of PBS was injected into the quadriceps femoralis muscle, followed immediately by injection of the 2 Needle Array to 5 mm depth encompassing the injection site and square-wave electroporation (ElectroSquarePorator 833, BTX-2 Needle Array 5 mm gap, Harvard Apparatus) delivered as eight pulses at 106 V for 20 ms with 200 ms intervals. ..

Article Title: Development and anticancer properties of Up284, a spirocyclic candidate ADRM1/RPN13 inhibitor
Article Snippet: .. A patch of CD1 mouse leg was shaved of hair and 10 μg 4Ub-FL plasmid in 20 μL of PBS was injected into the quadriceps femoralis muscle followed immediately by injection of the 2 Needle Array to 5 mm depth encompassing the injection site and square wave EP (ElectroSquarePorator 833, BTX-2 Needle array 5mm gap, Harvard apparatus) delivered as eight pulses at 106V for 20 ms with 200 ms intervals. ..

Injection:

Article Title: Chirality and asymmetry increase the potency of candidate ADRM1/RPN13 inhibitors
Article Snippet: .. A patch of CD1 mouse leg was shaved of hair and 10 μg 4Ub-FL plasmid in 20 μL of PBS was injected into the quadriceps femoralis muscle followed immediately by injection of the 2 Needle Array to 5 mm depth encompassing the injection site and square wave electroporation (ElectroSquarePorator 833, BTX-2 Needle array 5mm gap, Harvard apparatus) delivered as eight pulses at 106V for 20 ms with 200 ms intervals. ..

Article Title: Structure-function analyses of candidate small molecule RPN13 inhibitors with antitumor properties
Article Snippet: .. A patch of Balb/c mouse leg was shaved of hair and 10 μg 4Ub-FL plasmid in 20 μL of PBS was injected into the quadriceps femoralis muscle followed immediately by injection of the 2 Needle Array to 5 mm depth encompassing the injection site and square wave electroporation (ElectroSquarePorator 833, BTX-2 Needle array 5mm gap, Harvard apparatus) delivered as eight pulses at 106V for 20 ms with 200 ms intervals. ..

Article Title: Synthetic Antiangiogenic Vascular Endothelial Growth Factor—A Splice Variant Revascularizes Ischemic Muscle in Peripheral Artery Disease
Article Snippet: BACKGROUND: Alternative splicing in the eighth exon Cterminus of VEGFA (vascular endothelial growth factorA) results in the formation of proangiogenic VEGF165a and antiangiogenic VEGF165b isoforms.. The only known difference between these 2 isoform families is a 6amino acid switch from CDKPRR (in VEGF165a) to SLTRKD (in VEGF165b).. We have recently shown that VEGF165b can induce VEGFR2activation but fails to induce VEGFR1 (VEGF receptor 1)activation.

Article Title: Covalent Rpn13-Binding Inhibitors for the Treatment of Ovarian Cancer
Article Snippet: Gene gun particle-mediated DNA delivery was performed using a helium-driven gene gun (Bio-Rad, Hercules, CA), as described previously. .. For electroporation, a patch of the mouse leg was shaved and 10 μg of 4UbFL or FL DNA plasmid in 20 μL of PBS was injected into the quadriceps femoralis muscle, followed immediately by injection of the 2 Needle Array to 5 mm depth encompassing the injection site and square-wave electroporation (ElectroSquarePorator 833, BTX-2 Needle Array 5 mm gap, Harvard Apparatus) delivered as eight pulses at 106 V for 20 ms with 200 ms intervals. ..

Article Title: Development and anticancer properties of Up284, a spirocyclic candidate ADRM1/RPN13 inhibitor
Article Snippet: .. A patch of CD1 mouse leg was shaved of hair and 10 μg 4Ub-FL plasmid in 20 μL of PBS was injected into the quadriceps femoralis muscle followed immediately by injection of the 2 Needle Array to 5 mm depth encompassing the injection site and square wave EP (ElectroSquarePorator 833, BTX-2 Needle array 5mm gap, Harvard apparatus) delivered as eight pulses at 106V for 20 ms with 200 ms intervals. ..

Electroporation:

Article Title: Chirality and asymmetry increase the potency of candidate ADRM1/RPN13 inhibitors
Article Snippet: .. A patch of CD1 mouse leg was shaved of hair and 10 μg 4Ub-FL plasmid in 20 μL of PBS was injected into the quadriceps femoralis muscle followed immediately by injection of the 2 Needle Array to 5 mm depth encompassing the injection site and square wave electroporation (ElectroSquarePorator 833, BTX-2 Needle array 5mm gap, Harvard apparatus) delivered as eight pulses at 106V for 20 ms with 200 ms intervals. ..

Article Title: Structure-function analyses of candidate small molecule RPN13 inhibitors with antitumor properties
Article Snippet: .. A patch of Balb/c mouse leg was shaved of hair and 10 μg 4Ub-FL plasmid in 20 μL of PBS was injected into the quadriceps femoralis muscle followed immediately by injection of the 2 Needle Array to 5 mm depth encompassing the injection site and square wave electroporation (ElectroSquarePorator 833, BTX-2 Needle array 5mm gap, Harvard apparatus) delivered as eight pulses at 106V for 20 ms with 200 ms intervals. ..

Article Title: Covalent Rpn13-Binding Inhibitors for the Treatment of Ovarian Cancer
Article Snippet: Gene gun particle-mediated DNA delivery was performed using a helium-driven gene gun (Bio-Rad, Hercules, CA), as described previously. .. For electroporation, a patch of the mouse leg was shaved and 10 μg of 4UbFL or FL DNA plasmid in 20 μL of PBS was injected into the quadriceps femoralis muscle, followed immediately by injection of the 2 Needle Array to 5 mm depth encompassing the injection site and square-wave electroporation (ElectroSquarePorator 833, BTX-2 Needle Array 5 mm gap, Harvard Apparatus) delivered as eight pulses at 106 V for 20 ms with 200 ms intervals. ..

other:

Article Title: Skeletal muscle-enriched miRNAs are highly unstable in vivo and may be regulated in a Dicer-independent manner.
Article Snippet: Within 1 min of injection, an electrical field was delivered to the injected muscles with a SEN-3401 electric stimulator (Nihon Kohden, Tokyo, Japan) through a Model 532 two-needle array (BTX Instrument Division Harvard Apparatus, Inc., Holliston, MA, USA).

Article Title: Glycolytic PFKFB3 and Glycogenic UGP2 Axis Regulates Perfusion Recovery in Experimental Hind Limb Ischemia
Article Snippet: BACKGROUND: Despite being in an oxygen-rich environment, endothelial cells (ECs) use anaerobic glycolysis (Warburg effect) as the primary metabolic pathway for cellular energy needs.. PFKFB (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase)-3 regulates a critical enzymatic checkpoint in glycolysis and has been shown to induce angiogenesis.. This study builds on our efforts to determine the metabolic regulation of ischemic angiogenesis and perfusion recovery in the ischemic muscle.

Article Title: Proteomics of rimmed vacuoles define new risk allele in inclusion body myositis
Article Snippet: Two-needle array electrodes (450121) (Harvard Apparatus, Holliston, MA, USA) were inserted into the muscle immediately after DNA delivery for electroporation.



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