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Promega two steps kit
Two Steps Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two+step+detection+kit/two+step+kit/pm22771636-77-7-9
Average 90 stars, based on 1 article reviews
two steps kit - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Real-time Polymerase Chain Reaction:

Article Title: Inhibition of fatty acid synthase suppresses osteosarcoma cell invasion and migration via downregulation of the PI3K/Akt signaling pathway in vitro.
Article Snippet: RT-PCR and the Two-Step kit (Promega Corporation, Madison, WI, USA) were used to to obtain cDNA according to the manufacturer's instructions, which was then used as the template for amplification.

Article Title: Inhibition of fatty acid synthase suppresses U-2 OS cell invasion and migration via downregulating the activity of HER2/PI3K/AKT signaling pathway in vitro.
Article Snippet: 0006-291X/$ see front matter 2013 Elsevier Inc. All rights reserved. http://dx.doi.org/10.1016/j.bbrc.2013.09.024 ⇑ Corresponding author.. Address: Department of Orthopedics, First Affiliated Hospital of Nanchang University, Yong Wai Zheng Street 17, Nanchang, Jiangxi 330006, PR China.. E-mail address: zgm7977@163.com (Z.L.

Article Title: Interaction between fatty acid synthase and human epidermal growth receptor 2 (HER2) in osteosarcoma cells
Article Snippet: The Two-Step kit (Promega Corporation, Madison, WI, USA) was used to obtain cDNA according to the manufacturer’s instructions, which was then used as the template for amplification.

Article Title: MEK inhibition enhances the antitumor effect of radiation therapy in NF1-deficient glioblastoma
Article Snippet: Real-time PCR RNA was extracted from treated cells and reverse transcription performed as previously described(22). qPCR was performed using the Promega 2-step kit on a CFX96 Real-Time PCR System (Bio-Rad) according to the manufacturer’s instructions.

Article Title: Knockdown of ELMO3 Suppresses Growth, Invasion and Metastasis of Colorectal Cancer
Article Snippet: The total RNA concentration was determined, and then 1 μg of total RNA was reverse transcribed using the Two-Step kit (Promega Corporation, Madison, WI, USA) according to the manufacturer’s instructions.

Article Title: LY294002 inhibits the malignant phenotype of osteosarcoma cells by modulating the phosphatidylinositol 3‑kinase/Akt/fatty acid synthase signaling pathway in vitro.
Article Snippet: The Two-Step kit (Promega Corporation, Madison, WI, USA) was used to obtain cDNA according to the manufacturer's instructions, which was subsequently used as the template for amplification.

Article Title: Positive feedback regulation between Akt phosphorylation and fatty acid synthase expression in osteosarcoma.
Article Snippet: The two-step kit (Promega Corp., Madison, WI, USA) was used to to obtain cDNA according to the manufacturer's instructions, which was then used as the template for amplification.

Reverse Transcription:

Article Title: Inhibition of fatty acid synthase suppresses osteosarcoma cell invasion and migration via downregulation of the PI3K/Akt signaling pathway in vitro.
Article Snippet: RT-PCR and the Two-Step kit (Promega Corporation, Madison, WI, USA) were used to to obtain cDNA according to the manufacturer's instructions, which was then used as the template for amplification.

Article Title: Inhibition of fatty acid synthase suppresses U-2 OS cell invasion and migration via downregulating the activity of HER2/PI3K/AKT signaling pathway in vitro.
Article Snippet: 0006-291X/$ see front matter 2013 Elsevier Inc. All rights reserved. http://dx.doi.org/10.1016/j.bbrc.2013.09.024 ⇑ Corresponding author.. Address: Department of Orthopedics, First Affiliated Hospital of Nanchang University, Yong Wai Zheng Street 17, Nanchang, Jiangxi 330006, PR China.. E-mail address: zgm7977@163.com (Z.L.

Article Title: Interaction between fatty acid synthase and human epidermal growth receptor 2 (HER2) in osteosarcoma cells
Article Snippet: The Two-Step kit (Promega Corporation, Madison, WI, USA) was used to obtain cDNA according to the manufacturer’s instructions, which was then used as the template for amplification.

Article Title: MEK inhibition enhances the antitumor effect of radiation therapy in NF1-deficient glioblastoma
Article Snippet: Real-time PCR RNA was extracted from treated cells and reverse transcription performed as previously described(22). qPCR was performed using the Promega 2-step kit on a CFX96 Real-Time PCR System (Bio-Rad) according to the manufacturer’s instructions.

Article Title: Knockdown of ELMO3 Suppresses Growth, Invasion and Metastasis of Colorectal Cancer
Article Snippet: The total RNA concentration was determined, and then 1 μg of total RNA was reverse transcribed using the Two-Step kit (Promega Corporation, Madison, WI, USA) according to the manufacturer’s instructions.

Article Title: LY294002 inhibits the malignant phenotype of osteosarcoma cells by modulating the phosphatidylinositol 3‑kinase/Akt/fatty acid synthase signaling pathway in vitro.
Article Snippet: The Two-Step kit (Promega Corporation, Madison, WI, USA) was used to obtain cDNA according to the manufacturer's instructions, which was subsequently used as the template for amplification.

Article Title: Positive feedback regulation between Akt phosphorylation and fatty acid synthase expression in osteosarcoma.
Article Snippet: The two-step kit (Promega Corp., Madison, WI, USA) was used to to obtain cDNA according to the manufacturer's instructions, which was then used as the template for amplification.

Concentration Assay:

Article Title: Inhibition of fatty acid synthase suppresses osteosarcoma cell invasion and migration via downregulation of the PI3K/Akt signaling pathway in vitro.
Article Snippet: RT-PCR and the Two-Step kit (Promega Corporation, Madison, WI, USA) were used to to obtain cDNA according to the manufacturer's instructions, which was then used as the template for amplification.

Article Title: Inhibition of fatty acid synthase suppresses U-2 OS cell invasion and migration via downregulating the activity of HER2/PI3K/AKT signaling pathway in vitro.
Article Snippet: 0006-291X/$ see front matter 2013 Elsevier Inc. All rights reserved. http://dx.doi.org/10.1016/j.bbrc.2013.09.024 ⇑ Corresponding author.. Address: Department of Orthopedics, First Affiliated Hospital of Nanchang University, Yong Wai Zheng Street 17, Nanchang, Jiangxi 330006, PR China.. E-mail address: zgm7977@163.com (Z.L.

Article Title: Interaction between fatty acid synthase and human epidermal growth receptor 2 (HER2) in osteosarcoma cells
Article Snippet: The Two-Step kit (Promega Corporation, Madison, WI, USA) was used to obtain cDNA according to the manufacturer’s instructions, which was then used as the template for amplification.

Article Title: MEK inhibition enhances the antitumor effect of radiation therapy in NF1-deficient glioblastoma
Article Snippet: Real-time PCR RNA was extracted from treated cells and reverse transcription performed as previously described(22). qPCR was performed using the Promega 2-step kit on a CFX96 Real-Time PCR System (Bio-Rad) according to the manufacturer’s instructions.

Article Title: Knockdown of ELMO3 Suppresses Growth, Invasion and Metastasis of Colorectal Cancer
Article Snippet: The total RNA concentration was determined, and then 1 μg of total RNA was reverse transcribed using the Two-Step kit (Promega Corporation, Madison, WI, USA) according to the manufacturer’s instructions.

Article Title: LY294002 inhibits the malignant phenotype of osteosarcoma cells by modulating the phosphatidylinositol 3‑kinase/Akt/fatty acid synthase signaling pathway in vitro.
Article Snippet: The Two-Step kit (Promega Corporation, Madison, WI, USA) was used to obtain cDNA according to the manufacturer's instructions, which was subsequently used as the template for amplification.

Article Title: Positive feedback regulation between Akt phosphorylation and fatty acid synthase expression in osteosarcoma.
Article Snippet: The two-step kit (Promega Corp., Madison, WI, USA) was used to to obtain cDNA according to the manufacturer's instructions, which was then used as the template for amplification.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Inhibition of fatty acid synthase suppresses osteosarcoma cell invasion and migration via downregulation of the PI3K/Akt signaling pathway in vitro.
Article Snippet: RT-PCR and the Two-Step kit (Promega Corporation, Madison, WI, USA) were used to to obtain cDNA according to the manufacturer's instructions, which was then used as the template for amplification.

Article Title: Inhibition of fatty acid synthase suppresses U-2 OS cell invasion and migration via downregulating the activity of HER2/PI3K/AKT signaling pathway in vitro.
Article Snippet: 0006-291X/$ see front matter 2013 Elsevier Inc. All rights reserved. http://dx.doi.org/10.1016/j.bbrc.2013.09.024 ⇑ Corresponding author.. Address: Department of Orthopedics, First Affiliated Hospital of Nanchang University, Yong Wai Zheng Street 17, Nanchang, Jiangxi 330006, PR China.. E-mail address: zgm7977@163.com (Z.L.

Article Title: Interaction between fatty acid synthase and human epidermal growth receptor 2 (HER2) in osteosarcoma cells
Article Snippet: The Two-Step kit (Promega Corporation, Madison, WI, USA) was used to obtain cDNA according to the manufacturer’s instructions, which was then used as the template for amplification.

Article Title: MEK inhibition enhances the antitumor effect of radiation therapy in NF1-deficient glioblastoma
Article Snippet: Real-time PCR RNA was extracted from treated cells and reverse transcription performed as previously described(22). qPCR was performed using the Promega 2-step kit on a CFX96 Real-Time PCR System (Bio-Rad) according to the manufacturer’s instructions.

Article Title: Knockdown of ELMO3 Suppresses Growth, Invasion and Metastasis of Colorectal Cancer
Article Snippet: The total RNA concentration was determined, and then 1 μg of total RNA was reverse transcribed using the Two-Step kit (Promega Corporation, Madison, WI, USA) according to the manufacturer’s instructions.

Article Title: LY294002 inhibits the malignant phenotype of osteosarcoma cells by modulating the phosphatidylinositol 3‑kinase/Akt/fatty acid synthase signaling pathway in vitro.
Article Snippet: The Two-Step kit (Promega Corporation, Madison, WI, USA) was used to obtain cDNA according to the manufacturer's instructions, which was subsequently used as the template for amplification.

Article Title: Positive feedback regulation between Akt phosphorylation and fatty acid synthase expression in osteosarcoma.
Article Snippet: The two-step kit (Promega Corp., Madison, WI, USA) was used to to obtain cDNA according to the manufacturer's instructions, which was then used as the template for amplification.

Amplification:

Article Title: Inhibition of fatty acid synthase suppresses osteosarcoma cell invasion and migration via downregulation of the PI3K/Akt signaling pathway in vitro.
Article Snippet: RT-PCR and the Two-Step kit (Promega Corporation, Madison, WI, USA) were used to to obtain cDNA according to the manufacturer's instructions, which was then used as the template for amplification.

Article Title: Inhibition of fatty acid synthase suppresses U-2 OS cell invasion and migration via downregulating the activity of HER2/PI3K/AKT signaling pathway in vitro.
Article Snippet: 0006-291X/$ see front matter 2013 Elsevier Inc. All rights reserved. http://dx.doi.org/10.1016/j.bbrc.2013.09.024 ⇑ Corresponding author.. Address: Department of Orthopedics, First Affiliated Hospital of Nanchang University, Yong Wai Zheng Street 17, Nanchang, Jiangxi 330006, PR China.. E-mail address: zgm7977@163.com (Z.L.

Article Title: Interaction between fatty acid synthase and human epidermal growth receptor 2 (HER2) in osteosarcoma cells
Article Snippet: The Two-Step kit (Promega Corporation, Madison, WI, USA) was used to obtain cDNA according to the manufacturer’s instructions, which was then used as the template for amplification.

Article Title: MEK inhibition enhances the antitumor effect of radiation therapy in NF1-deficient glioblastoma
Article Snippet: Real-time PCR RNA was extracted from treated cells and reverse transcription performed as previously described(22). qPCR was performed using the Promega 2-step kit on a CFX96 Real-Time PCR System (Bio-Rad) according to the manufacturer’s instructions.

Article Title: Knockdown of ELMO3 Suppresses Growth, Invasion and Metastasis of Colorectal Cancer
Article Snippet: The total RNA concentration was determined, and then 1 μg of total RNA was reverse transcribed using the Two-Step kit (Promega Corporation, Madison, WI, USA) according to the manufacturer’s instructions.

Article Title: LY294002 inhibits the malignant phenotype of osteosarcoma cells by modulating the phosphatidylinositol 3‑kinase/Akt/fatty acid synthase signaling pathway in vitro.
Article Snippet: The Two-Step kit (Promega Corporation, Madison, WI, USA) was used to obtain cDNA according to the manufacturer's instructions, which was subsequently used as the template for amplification.

Article Title: Positive feedback regulation between Akt phosphorylation and fatty acid synthase expression in osteosarcoma.
Article Snippet: The two-step kit (Promega Corp., Madison, WI, USA) was used to to obtain cDNA according to the manufacturer's instructions, which was then used as the template for amplification.



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