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tris borate edta buffer  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology tris borate edta buffer
    Summary of the experimental conditions and antibodies used for IF analysis.
    Tris Borate Edta Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tris+buffer+solution/Tris-EDTA+buffer+solution/pmc07926545-15-4-17
    Average 93 stars, based on 79 article reviews
    tris borate edta buffer - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Picturing Breast Cancer Brain Metastasis Development to Unravel Molecular Players and Cellular Crosstalk"

    Article Title: Picturing Breast Cancer Brain Metastasis Development to Unravel Molecular Players and Cellular Crosstalk

    Journal: Cancers

    doi: 10.3390/cancers13040910

    Summary of the experimental conditions and antibodies used for IF analysis.
    Figure Legend Snippet: Summary of the experimental conditions and antibodies used for IF analysis.

    Techniques Used: Blocking Assay, Plasmid Preparation

    Related Articles

    Saline:

    Article Title: Targeting PIM1 by Bruceine D attenuates skin fibrosis via myofibroblast ferroptosis.
    Article Snippet: Skin pan-fibrosis diseases—such as hypertrophic scar (HS), keloid scar (KS), and systemic sclerosis (SSc)—pose significant threats to patients’ health and quality of life.. In this study, the authors conducted both in vivo and in vitro experiments and discovered that the serine/threonine kinase PIM1 is upregulated in the myofibroblasts of human HS, KS, and SSc tissues, as well as in various animal models of skin fibrosis.. Overexpression of PIM1 enhanced the profibrotic phenotypes of human hypertrophic scar fibroblasts (HSFs), which serve as key effector cells in the pathogenesis of skin pan-fibrosis diseases.

    Article Title: Deciphering the Intricate Relationship Between Macrophages, Pigmentation, and Prognosis in Uveal Melanoma.
    Article Snippet: High pigmentation and the abundance of M2 macrophages have been identified as negative predictors in uveal melanoma (UM).. Risk factors associated with UM that are prevalent in high-risk White populations are still present, although less common, in relatively low-risk Asian populations.. Research indicates that proangiogenic M2 macrophages and monosomy 3 play significant roles in UM progression.

    Article Title: Targeting PIM1 by Bruceine D attenuates skin fibrosis via myofibroblast ferroptosis
    Article Snippet: .. After antigen retrieval within Tris-EDTA buffer (pH = 9.0), 5 % goat serum-phosphate buffered saline (PBS) was added to block samples, followed by overnight co-staining using primary antibody against PIM1 (1:50, SC-13513, Santa Cruz) under 4 °C and on the following day, HRP-labeled secondary antibody (ComWin, Beijing, China) was added to incubate sections for 60 min under ambient temperature. .. After washing thrice in PBS, DAB reagent (DAKO, Denmark) was applied in section development, whereas hematoxylin was introduced for counterstaining.

    Blocking Assay:

    Article Title: Targeting PIM1 by Bruceine D attenuates skin fibrosis via myofibroblast ferroptosis.
    Article Snippet: Skin pan-fibrosis diseases—such as hypertrophic scar (HS), keloid scar (KS), and systemic sclerosis (SSc)—pose significant threats to patients’ health and quality of life.. In this study, the authors conducted both in vivo and in vitro experiments and discovered that the serine/threonine kinase PIM1 is upregulated in the myofibroblasts of human HS, KS, and SSc tissues, as well as in various animal models of skin fibrosis.. Overexpression of PIM1 enhanced the profibrotic phenotypes of human hypertrophic scar fibroblasts (HSFs), which serve as key effector cells in the pathogenesis of skin pan-fibrosis diseases.

    Article Title: Targeting PIM1 by Bruceine D attenuates skin fibrosis via myofibroblast ferroptosis
    Article Snippet: .. After antigen retrieval within Tris-EDTA buffer (pH = 9.0), 5 % goat serum-phosphate buffered saline (PBS) was added to block samples, followed by overnight co-staining using primary antibody against PIM1 (1:50, SC-13513, Santa Cruz) under 4 °C and on the following day, HRP-labeled secondary antibody (ComWin, Beijing, China) was added to incubate sections for 60 min under ambient temperature. .. After washing thrice in PBS, DAB reagent (DAKO, Denmark) was applied in section development, whereas hematoxylin was introduced for counterstaining.

    Immunohistochemistry:

    Article Title: Deciphering the Intricate Relationship Between Macrophages, Pigmentation, and Prognosis in Uveal Melanoma.
    Article Snippet: High pigmentation and the abundance of M2 macrophages have been identified as negative predictors in uveal melanoma (UM).. Risk factors associated with UM that are prevalent in high-risk White populations are still present, although less common, in relatively low-risk Asian populations.. Research indicates that proangiogenic M2 macrophages and monosomy 3 play significant roles in UM progression.

    other:

    Article Title: Role of early growth response-1 as a tumor suppressor in oral squamous cell carcinoma.
    Article Snippet: The resulting solution was analyzed using a flow cytometer (CyAn ADP, BECKMAN COULTER) and Cell Quest software (BD GmbH, Heidelberg, Germany).

    Article Title: Chronic starvation induces microglial cell depletion in an activity-based anorexia model
    Article Snippet: GFAP , Mouse , 1:2000 , Tris-EDTA buffer (10 min) , Santa Cruz, USA , Astroglia , sc-33,673.

    Article Title: A case of anaplastic oligodendroglioma in a rabbit ( Oryctolagus cuniculus )
    Article Snippet: AC in citrate buffer/10 min AC in citrate buffer/10 min AC in Tris-EDTA buffer/10 min Merck Millipore Merck Millipore Merck Millipore Santa Cruz Dako Manufacturer Cell Signaling Technology (Denver, MA, U.S.A.) FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan) Dako (Santa Clara, CA, U.S.A.) Merck Millipore (Darmstadt, Germany) AC in citrate buffer/10 min AC in citrate buffer/10 min Antigen retrieval AC in citrate buffer/10 min AC in citrate buffer/10 min AC in citrate buffer/10 min AC in citrate buffer/10 min Santa Cruz (Dallas, TX, U.S.A.) Supplementary Fig. 2.

    Article Title: A case of anaplastic oligodendroglioma in a rabbit (Oryctolagus cuniculus).
    Article Snippet: AC in citrate buffer/10 min AC in citrate buffer/10 min AC in Tris-EDTA buffer/10 min Merck Millipore Merck Millipore Merck Millipore Santa Cruz Dako Manufacturer Cell Signaling Technology (Denver, MA, U.S.A.) FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan) Dako (Santa Clara, CA, U.S.A.) Merck Millipore (Darmstadt, Germany) AC in citrate buffer/10 min AC in citrate buffer/10 min Antigen retrieval AC in citrate buffer/10 min AC in citrate buffer/10 min AC in citrate buffer/10 min AC in citrate buffer/10 min Santa Cruz (Dallas, TX, U.S.A.) Supplementary Fig. 2.

    Staining:

    Article Title: Chronic starvation induces microglial cell depletion in an activity-based anorexia model.
    Article Snippet: .. Staining for the following Antibody Antibody host Concentration Antigen-retrieval Manufacturer Target cells Catalog number GFAP Mouse 1:2000 Tris-EDTA buffer (10 min) Santa Cruz, USA Astroglia sc-33,673 IBA1 Rabbit 1:10000 Tris-EDTA buffer (10 min) Wako, Germany Microglia 019-19741 OLIG2 Mouse 1:1000 Tris-EDTA buffer (20 min) Millipore, Germany Mature and premature oligodendrocytes MABN50 MAP2 Rrabbit 1:1000 Citrate buffer (20 min) cell signaling, Netherlands Neurons 8707 Ki-67 Rabbit 1:1500 Citrate buffer (10 min) Abcam, UK Proliferating cells ab16667 APC Mouse 1:250 Tris-EDTA buffer (20 min) Millipore, Germany Mature oligodendrocytes OP80 Table 1. ..

    Concentration Assay:

    Article Title: Chronic starvation induces microglial cell depletion in an activity-based anorexia model.
    Article Snippet: .. Staining for the following Antibody Antibody host Concentration Antigen-retrieval Manufacturer Target cells Catalog number GFAP Mouse 1:2000 Tris-EDTA buffer (10 min) Santa Cruz, USA Astroglia sc-33,673 IBA1 Rabbit 1:10000 Tris-EDTA buffer (10 min) Wako, Germany Microglia 019-19741 OLIG2 Mouse 1:1000 Tris-EDTA buffer (20 min) Millipore, Germany Mature and premature oligodendrocytes MABN50 MAP2 Rrabbit 1:1000 Citrate buffer (20 min) cell signaling, Netherlands Neurons 8707 Ki-67 Rabbit 1:1500 Citrate buffer (10 min) Abcam, UK Proliferating cells ab16667 APC Mouse 1:250 Tris-EDTA buffer (20 min) Millipore, Germany Mature oligodendrocytes OP80 Table 1. ..



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