transcriptome analysis (BioSpyder Technologies)
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Transcriptome Analysis, supplied by BioSpyder Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transcriptomic+analysis/transcriptome+analysis/pmc09071099-125-15-18
Average 90 stars, based on 1 article reviews
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1) Product Images from "Towards an advanced testing strategy for genotoxicity using image-based 2D and 3D HepG2 DNA damage response fluorescent protein reporters"
Article Title: Towards an advanced testing strategy for genotoxicity using image-based 2D and 3D HepG2 DNA damage response fluorescent protein reporters
Journal: Mutagenesis
doi: 10.1093/mutage/geab031
Figure Legend Snippet: Hepatocyte maturation status of four different HepG2 models. (A) Bright field pictures showing morphological differences between the different models; HepG2 cultured in 2D and normal DMEM medium, HepG2 cultured in 2D and AAGLY medium, HepG2 cultured in 3D and normal DMEM medium, HepG2 cultured in 3D and AAGLY medium from left to right. (B) Gene expression profiles of some hepatocyte markers ( SERPINA1 and ALB ), CYP enzymes ( 3A4 , 3A7 and 1B1 ) and transporters ( UGT1A1 and SLC10A1 ) and the housekeeping genes ( GAPDH ). Gene expression values are relative to model 1 (HepG2 cultured in 2D and normal DMEM medium) and benchmarked to a pool of 10 different donors of cryopreserved PHHs (10×). N = 6; error bars represent SD; significance levels represented as * P adj < 0.05, ** P adj < 0.01 and *** P adj < 0.001.
Techniques Used: Cell Culture, Gene Expression
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Cell Culture:Article Title: Towards an advanced testing strategy for genotoxicity using image-based 2D and 3D HepG2 DNA damage response fluorescent protein reporters Article Snippet: day, cells were exposed to cisplatin in a wide concentration range for 8 or 24 h followed by sample collection using 1× BNN lysis buffer (BioSpyder). .. Samples were lysed for 15 min at room temperature, stored at −80°C and shipped for Gene Expression:Article Title: Towards an advanced testing strategy for genotoxicity using image-based 2D and 3D HepG2 DNA damage response fluorescent protein reporters Article Snippet: day, cells were exposed to cisplatin in a wide concentration range for 8 or 24 h followed by sample collection using 1× BNN lysis buffer (BioSpyder). .. Samples were lysed for 15 min at room temperature, stored at −80°C and shipped for |
