act (analysis customization toolkit)  (Custo Med GmbH)

Journal: Neural Regeneration Research

Article Title: Single-cell RNA sequencing of the post–spinal cord injury dorsal root ganglia in cynomolgus monkeys: Elucidation of the cellular immune microenvironment of the central nervous system

doi: 10.4103/NRR.NRR-D-24-00974

Figure Lengend Snippet: Single-cell dataset with reduced dimension clustering and cell type identification in DRG cells from a cynomolgus monkey with SCI. (A) Schematic representation of the experimental workflow. (B) Cluster analysis of cell groups, and t-SNE distribution showing cluster analysis groupings. Each point represents a cell, and cells that are close in distance are considered to be of the same type. Different groups of cells are distinguished by different colors and numbers. (C) t-SNE plot of all cells collected from the DRG following SCI. Cells are colored and annotated by cell type. (D) Heatmap of distinct genes with high and low expression enriched in each cell type. (E) t-SNE distribution of different cell types in the DRG and spinal cord following SCI. DRG: Dorsal root ganglia; SCI: spinal cord injury; t-SNE: t-distributed stochastic neighbor embedding.

Article Snippet: For the 10X Genomics data, the Cell Ranger Single-Cell toolkit (v3.0.0; 10X Genomics, Majorbio Co., Ltd., Shanghai, China) was employed to align the reads and generate the gene–cell unique molecular identifier (UMI) matrix for each sample ( https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest ).

Techniques: Single Cell, Expressing