tnt wash buffer (NEN Life Science)

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NEN Life Science tnt wash buffer
Tnt Wash Buffer, supplied by NEN Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tnt+buffer/tnt+buffer/pmc01514466-113-16-19
Average 90 stars, based on 1 article reviews

tnt wash buffer - by Bioz Stars, 2026-11

90/100 stars

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Amplification:

Article Title: Endothelial-Directed Hepatic Regeneration After Partial Hepatectomy
Article Snippet: After all incubations, except for the TNB blocking step, the sections were washed three times with TNT wash buffer (NEN Life Sciences). .. The TNB buffer, TNT buffer, biotinylated-tyramide solution, amplification diluent, and streptavidin-AP were used according to the instructions of the NEN TSA indirect kit (catalogue #NEL 700A, NEN Life Sciences). ..

Article Title: Expression of the novel epithelial Ca2+ channel ECaC1 in rat pancreatic islets.
Article Snippet: The epithelial Ca 2 channel, ECaC1, is primarily expressed in the apical membrane of vitamin D-responsive tissues.. This study characterizes for the first time the presence of this novel channel in pancreatic tissue by reverse transcriptase-polymerase chain reaction and immunohistochemistry.. In addition, the expression of ECaC1 was investigated in an animal model for Type 2 diabetes mellitus, the Zucker diabetic fatty (ZDF) rat.

Article Title: TTF-1 regulates growth hormone and prolactin transcription in the anterior pituitary gland.
Article Snippet: Thyroid transcription factor 1 (TTF-1) is required for morphogenesis of the fetal diencephalon.. Previous reports showed that mice carrying a TTF-1 null mutation lacked normal development of the pituitary gland.. In this study, a role for TTF-1 in the regulation of growth hormone and prolactin transcription was identified.

Article Title: Expression and role of TTF-1 in the rat suprachiasmatic nucleus.
Article Snippet: a Department of Biological Sciences, College of Natural Sciences, University of Ulsan, San29, Mugeodong, Namgu, Ulsan 680-749, South Korea b Biomedical Research Center, College of Medicine, University of Ulsan, Ulsan 682-714, South Korea c Hangang Burn Center and Department of General Surgery, Hallym University College of Medicine, Seoul 94-200, South Korea d School of Biotechnology and Biomedical Sciences, Inje University, Kimhae 621-749, South Korea

Incubation:

Staining:

Blocking Assay:

Article Title: Endothelial-Directed Hepatic Regeneration After Partial Hepatectomy
Article Snippet: .. After all incubations, except for the TNB blocking step, the sections were washed three times with TNT wash buffer (NEN Life Sciences). .. The TNB buffer, TNT buffer, biotinylated-tyramide solution, amplification diluent, and streptavidin-AP were used according to the instructions of the NEN TSA indirect kit (catalogue #NEL 700A, NEN Life Sciences).

Western Blot:

Article Title: CD4 + T-Lymphocyte and Immunoglobulin G2 Responses in Calves Immunized with Anaplasma marginale Outer Membranes and Protected against Homologous Challenge
Article Snippet: The reactions were then labeled for 1 h at room temperature with peroxidase-conjugated affinity-purified donkey anti-mouse IgG (heavy and light chains) (Jackson Immunoresearch Laboratories, West Grove, Pa.) diluted 1:5,000 in TNT buffer containing 1% horse serum. .. The membranes were washed repeatedly with TNT buffer, and the chemiluminescence was developed with a Renaissance Western blot chemiluminescence reagent (NEN Life Science Products, Boston, Mass.) according to the manufacturer’s instructions. ..

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