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tmprss2 sequence  (Addgene inc)


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    Structured Review

    Addgene inc tmprss2 sequence
    Fig. 1. Expression of CD13 and <t>TMPRSS2</t> in engineered A549 cells. 30 μg of protein from cell lysates was loaded onto a 4%–12% Bis-Tris gel and sepa rated by SDS-PAGE before expressions of (A) CD13 and (B) TMPRSS2 were analyzed by Western Blot. GAPDH detection is included as the loading control. WT A549 cells are included as a negative control, whereas HAE lysates are included as a positive control for TMPRSS2.
    Tmprss2 Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tmprss2+sequence/pLEX307-TMPRSS2-blast+(Plasmid+%23158458)/pm37778059-45-35-47
    Average 93 stars, based on 7 article reviews
    tmprss2 sequence - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "An engineered A549 cell line expressing CD13 and TMPRSS2 is permissive to clinical isolate of human coronavirus 229E."

    Article Title: An engineered A549 cell line expressing CD13 and TMPRSS2 is permissive to clinical isolate of human coronavirus 229E.

    Journal: Virology

    doi: 10.1016/j.virol.2023.109889

    Fig. 1. Expression of CD13 and TMPRSS2 in engineered A549 cells. 30 μg of protein from cell lysates was loaded onto a 4%–12% Bis-Tris gel and sepa rated by SDS-PAGE before expressions of (A) CD13 and (B) TMPRSS2 were analyzed by Western Blot. GAPDH detection is included as the loading control. WT A549 cells are included as a negative control, whereas HAE lysates are included as a positive control for TMPRSS2.
    Figure Legend Snippet: Fig. 1. Expression of CD13 and TMPRSS2 in engineered A549 cells. 30 μg of protein from cell lysates was loaded onto a 4%–12% Bis-Tris gel and sepa rated by SDS-PAGE before expressions of (A) CD13 and (B) TMPRSS2 were analyzed by Western Blot. GAPDH detection is included as the loading control. WT A549 cells are included as a negative control, whereas HAE lysates are included as a positive control for TMPRSS2.

    Techniques Used: Expressing, SDS Page, Western Blot, Control, Negative Control, Positive Control

    Related Articles

    Recombinant:

    Article Title: Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform
    Article Snippet: Antibody , Anti-SARS-CoV-2 ORF8 (rabbit polyclonal) , Novus Biologicals , Cat# NBP3-07972; Lot# 25966-2102 , WB (1:1000) ICC (1:1000). .. Recombinant DNA reagent , pLEX307-TMPRSS2-blast (plasmid) , Addgene , Cat# 158458 , –. .. Recombinant DNA reagent , pUC19_CoV-2_Linker , This study , Addgene plasmid #211731 , Plasmid expressing 5’ and 3’ regions for direct rescue.

    Plasmid Preparation:

    Article Title: Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform
    Article Snippet: Antibody , Anti-SARS-CoV-2 ORF8 (rabbit polyclonal) , Novus Biologicals , Cat# NBP3-07972; Lot# 25966-2102 , WB (1:1000) ICC (1:1000). .. Recombinant DNA reagent , pLEX307-TMPRSS2-blast (plasmid) , Addgene , Cat# 158458 , –. .. Recombinant DNA reagent , pUC19_CoV-2_Linker , This study , Addgene plasmid #211731 , Plasmid expressing 5’ and 3’ regions for direct rescue.

    Article Title: High protection and transmission-blocking immunity elicited by single-cycle SARS-CoV-2 vaccine in hamsters
    Article Snippet: .. Human TMPRSS2 expression in Vero-E2T and Vero E6 cells (Vero E6-TMPRSS2) was achieved by infecting the cells with a 2nd generation lentiviral vector pLEX307-TMPRSS2-blast (Addgene plasmid #158458) as a transfer vector. ..

    Article Title: Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform
    Article Snippet: .. Vero E6-TMPRSS2 cells were generated by transduction with a second-generation lentiviral vector pLEX307-TMPRSS2-blast (Addgene plasmid #158458) and selected for 2 wk in DMEM containing 20 μg/mL of Blasticidin (Cat# SBR00022, Sigma-Aldrich). .. Virus stocks of the initial Wuhan strain of SARS-CoV-2 were provided by G. Kochs, University of Freiburg, Germany (SARS-CoV_FR-3 [GenBank OR018857]; SARS-CoV_Muc [GenBank OR018856]).

    Article Title: High protection and transmission-blocking immunity elicited by single-cycle SARS-CoV-2 vaccine in hamsters.
    Article Snippet: .. Human TMPRSS2 expression in Vero-E2T andVero E6 cells (Vero E6-TMPRSS2) was achieved by infecting the cells with a 2nd generation lentiviral vector pLEX307-TMPRSS2-blast (Addgene plasmid #158458) as a transfer vector. ..

    Article Title: The proton-activated chloride channel inhibits SARS-CoV-2 spike protein-mediated viral entry through the endosomal pathway
    Article Snippet: .. 6 μg of ACE2 plasmid (pLENTI_ACE2_PURO, Addgene 155295), TMPRSS2 plasmid (pLEX307-TMPRSS2-blast, Addgene158458) or hPAC-mCherry were co-transfected with 2 μg of packaging plasmids [pVSV-G (pMD2.G, Addgene 12259), pMDL (pMDLg/pRRE, Addgene 12251) and pRSV (pRSV-REV, Addgene 12253)]. .. To generate VSV-G pseudoviral particles, we transfected a 15 cm dish of HEK 293T cells with 3.5 μg of VSV-G along with 3.5 μg of packaging plasmids pMDL and pRSV, and 16 μg of luciferase construct (pHIV-Luc-ZsGreen, Addgene 39196).

    Next-Generation Sequencing:

    Article Title: High protection and transmission-blocking immunity elicited by single-cycle SARS-CoV-2 vaccine in hamsters.
    Article Snippet: .. We thank G. Kochs, Freiburg, GER, and the Bundeswehr Institute of Microbiology, Munich, GER for providing the SARS-CoV-2 Wuhan isolates and Martin Daeumer, Alex Thielen (Seq-It GmbH, Kaiserslautern, GER) and Adrian Egli & team (USB, Basel, CH) for expert NGS support. pCMVR8.74 and pMD2.G were a gift from Didier Trono (RRID: Addgene_12259), pLEX307-TMPRSS2-blast was a gift from Alejandro Chavez & Sho Iketani (RRID: Addgene_158458). pHR_PGK-hACE2 was a gift from Brad Rosenberg (RRID: Addgene_161612). .. Vero E6 cells and synthetic SARS-CoV-2 virus (Wuhan-1, GenBank No. MT108784) were kindly provided by Volker Thiel.

    Expressing:

    Article Title: High protection and transmission-blocking immunity elicited by single-cycle SARS-CoV-2 vaccine in hamsters
    Article Snippet: .. Human TMPRSS2 expression in Vero-E2T and Vero E6 cells (Vero E6-TMPRSS2) was achieved by infecting the cells with a 2nd generation lentiviral vector pLEX307-TMPRSS2-blast (Addgene plasmid #158458) as a transfer vector. ..

    Article Title: High protection and transmission-blocking immunity elicited by single-cycle SARS-CoV-2 vaccine in hamsters.
    Article Snippet: .. Human TMPRSS2 expression in Vero-E2T andVero E6 cells (Vero E6-TMPRSS2) was achieved by infecting the cells with a 2nd generation lentiviral vector pLEX307-TMPRSS2-blast (Addgene plasmid #158458) as a transfer vector. ..

    Generated:

    Article Title: Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform
    Article Snippet: .. Vero E6-TMPRSS2 cells were generated by transduction with a second-generation lentiviral vector pLEX307-TMPRSS2-blast (Addgene plasmid #158458) and selected for 2 wk in DMEM containing 20 μg/mL of Blasticidin (Cat# SBR00022, Sigma-Aldrich). .. Virus stocks of the initial Wuhan strain of SARS-CoV-2 were provided by G. Kochs, University of Freiburg, Germany (SARS-CoV_FR-3 [GenBank OR018857]; SARS-CoV_Muc [GenBank OR018856]).

    Article Title: High protection and transmission-blocking immunity elicited by single-cycle SARS-CoV-2 vaccine in hamsters.
    Article Snippet: The plasmids required for the generation of second-generation lentiviruses were obtained from Addgene. .. Lentiviruses were generated by transfecting HEK-293T cells with pCMVR8.74 (RRID:Addgene_22036), pMD2.G (RRID:Addgene_12259), and pLEX307-TMPRSS2-blast (RRID:Addgene_158458) plasmids. ..

    Transduction:

    Article Title: Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform
    Article Snippet: .. Vero E6-TMPRSS2 cells were generated by transduction with a second-generation lentiviral vector pLEX307-TMPRSS2-blast (Addgene plasmid #158458) and selected for 2 wk in DMEM containing 20 μg/mL of Blasticidin (Cat# SBR00022, Sigma-Aldrich). .. Virus stocks of the initial Wuhan strain of SARS-CoV-2 were provided by G. Kochs, University of Freiburg, Germany (SARS-CoV_FR-3 [GenBank OR018857]; SARS-CoV_Muc [GenBank OR018856]).



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    Fig. 1. Expression of CD13 and <t>TMPRSS2</t> in engineered A549 cells. 30 μg of protein from cell lysates was loaded onto a 4%–12% Bis-Tris gel and sepa rated by SDS-PAGE before expressions of (A) CD13 and (B) TMPRSS2 were analyzed by Western Blot. GAPDH detection is included as the loading control. WT A549 cells are included as a negative control, whereas HAE lysates are included as a positive control for TMPRSS2.
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    Image Search Results


    Experimental and modelled ECD spectrum of the TMPRSS2 mRNA G4 in solution.

    Journal: bioRxiv

    Article Title: Resolving the Structure of a Guanine Quadruplex in TMPRSS2 Messenger RNA by Circular Dichroism and Molecular Modeling

    doi: 10.1101/2024.07.29.605618

    Figure Lengend Snippet: Experimental and modelled ECD spectrum of the TMPRSS2 mRNA G4 in solution.

    Article Snippet: For this experiment we used the TMPRSS2 sequence 5’-GGGCGGGCGGCCUGCAGGGACAUGGG-3’ (IDT Technologies).

    Techniques:

    Journal: bioRxiv

    Article Title: Resolving the Structure of a Guanine Quadruplex in TMPRSS2 Messenger RNA by Circular Dichroism and Molecular Modeling

    doi: 10.1101/2024.07.29.605618

    Figure Lengend Snippet:

    Article Snippet: For this experiment we used the TMPRSS2 sequence 5’-GGGCGGGCGGCCUGCAGGGACAUGGG-3’ (IDT Technologies).

    Techniques:

    Fig. 1. Expression of CD13 and TMPRSS2 in engineered A549 cells. 30 μg of protein from cell lysates was loaded onto a 4%–12% Bis-Tris gel and sepa rated by SDS-PAGE before expressions of (A) CD13 and (B) TMPRSS2 were analyzed by Western Blot. GAPDH detection is included as the loading control. WT A549 cells are included as a negative control, whereas HAE lysates are included as a positive control for TMPRSS2.

    Journal: Virology

    Article Title: An engineered A549 cell line expressing CD13 and TMPRSS2 is permissive to clinical isolate of human coronavirus 229E.

    doi: 10.1016/j.virol.2023.109889

    Figure Lengend Snippet: Fig. 1. Expression of CD13 and TMPRSS2 in engineered A549 cells. 30 μg of protein from cell lysates was loaded onto a 4%–12% Bis-Tris gel and sepa rated by SDS-PAGE before expressions of (A) CD13 and (B) TMPRSS2 were analyzed by Western Blot. GAPDH detection is included as the loading control. WT A549 cells are included as a negative control, whereas HAE lysates are included as a positive control for TMPRSS2.

    Article Snippet: The mix consisted of 27 μg of PEI (Sigma-Aldrich), 9 μg of a vector carrying either the CD13/aminopeptidase N sequence (pLEX307-APN-G418 was a gift from Alejandro Chavez & Sho Iketani; Addgene plasmid #158456) or the TMPRSS2 sequence (pLEX307-TMPRSS2-blast was a gift from Alejandro Chavez & Sho Iketani; Addgene plasmid #158458).

    Techniques: Expressing, SDS Page, Western Blot, Control, Negative Control, Positive Control

    Binding energy of top ranked plant secondary metabolites against four targets of SARS-CoV-2 pathogenesis and their physicochemical properties.

    Journal: Scientific Reports

    Article Title: In silico studies evidenced the role of structurally diverse plant secondary metabolites in reducing SARS-CoV-2 pathogenesis

    doi: 10.1038/s41598-020-77602-0

    Figure Lengend Snippet: Binding energy of top ranked plant secondary metabolites against four targets of SARS-CoV-2 pathogenesis and their physicochemical properties.

    Article Snippet: The TMPRSS2 sequence (NP_001128571.1) was retrieved from the National Center for Biotechnology Information (NCBI) protein database.

    Techniques: Binding Assay

    ( a ) Structural activity relationship: Correlation of canonical SMILES structure similarity (data points are joined by colored lines) and binding energy (represented in different color shades of data point) of selected plant secondary metabolites (PSM) evaluated against SARS-CoV-2 TMPRSS2 using Data Warrior software. Structurally similar molecules are grouped in dotted lines and a representative molecule with low binding energy (kcal/mol) (values in parenthesis) is represented in box. Among top ranked PSM studied, > 32% found belonging to Flavonoid glucoside and other major groups are ellagitannins and triterpenoids. ( b ) Data analysis of selected PSM against SARS-CoV-2 TMPRSS2. ( A ) Bioavailability radar chart representing lipophilicity (LIPO), Molecular weight (SIZE), Topological polar surface area (POLAR), Solubility (INSOLU), Flexibility (FLEX) and Saturation (INSATU) along with Bioavailability score (BAS) of selected molecules, ( B ) 3D visualization of protein–ligand interaction using PyMOL (selected amino acid residue of target site of protein are colored in cyan, and ( C ) 2D visualization of different types of interactions between ligand and target site of protein using Discovery Studio software (different types of interactions are represented in color codes).

    Journal: Scientific Reports

    Article Title: In silico studies evidenced the role of structurally diverse plant secondary metabolites in reducing SARS-CoV-2 pathogenesis

    doi: 10.1038/s41598-020-77602-0

    Figure Lengend Snippet: ( a ) Structural activity relationship: Correlation of canonical SMILES structure similarity (data points are joined by colored lines) and binding energy (represented in different color shades of data point) of selected plant secondary metabolites (PSM) evaluated against SARS-CoV-2 TMPRSS2 using Data Warrior software. Structurally similar molecules are grouped in dotted lines and a representative molecule with low binding energy (kcal/mol) (values in parenthesis) is represented in box. Among top ranked PSM studied, > 32% found belonging to Flavonoid glucoside and other major groups are ellagitannins and triterpenoids. ( b ) Data analysis of selected PSM against SARS-CoV-2 TMPRSS2. ( A ) Bioavailability radar chart representing lipophilicity (LIPO), Molecular weight (SIZE), Topological polar surface area (POLAR), Solubility (INSOLU), Flexibility (FLEX) and Saturation (INSATU) along with Bioavailability score (BAS) of selected molecules, ( B ) 3D visualization of protein–ligand interaction using PyMOL (selected amino acid residue of target site of protein are colored in cyan, and ( C ) 2D visualization of different types of interactions between ligand and target site of protein using Discovery Studio software (different types of interactions are represented in color codes).

    Article Snippet: The TMPRSS2 sequence (NP_001128571.1) was retrieved from the National Center for Biotechnology Information (NCBI) protein database.

    Techniques: Activity Assay, Binding Assay, Software, Molecular Weight, Solubility, Residue