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tissue microarray based immunohistochemistry analysis  (Human Protein Atlas)

 
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    Human Protein Atlas tissue microarray based immunohistochemistry analysis
    Cell type specific genes demonstrated at mRNA level. (A) Heat map of genes with cell type specific expression. (B) Heat map of selected genes with endothelial or epithelial cell type specific expression. (C) <t>Immunohistochemistry</t> staining of protein C1orf116 across human tissues showing its epithelial cell type specific expression. The panel shows a magnified view (20×) from tissue microarrays.
    Tissue Microarray Based Immunohistochemistry Analysis, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tissue-microarray+based+immunohistochemistry+datasets/data+microarray+tissue/pmc12802902-14-17-1
    Average 86 stars, based on 1 article reviews
    tissue microarray based immunohistochemistry analysis - by Bioz Stars, 2026-09
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    1) Product Images from "A transcriptomic and proteomic map of primary human cell types"

    Article Title: A transcriptomic and proteomic map of primary human cell types

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkaf1498

    Cell type specific genes demonstrated at mRNA level. (A) Heat map of genes with cell type specific expression. (B) Heat map of selected genes with endothelial or epithelial cell type specific expression. (C) Immunohistochemistry staining of protein C1orf116 across human tissues showing its epithelial cell type specific expression. The panel shows a magnified view (20×) from tissue microarrays.
    Figure Legend Snippet: Cell type specific genes demonstrated at mRNA level. (A) Heat map of genes with cell type specific expression. (B) Heat map of selected genes with endothelial or epithelial cell type specific expression. (C) Immunohistochemistry staining of protein C1orf116 across human tissues showing its epithelial cell type specific expression. The panel shows a magnified view (20×) from tissue microarrays.

    Techniques Used: Expressing, Immunohistochemistry, Staining

    Related Articles

    Transcriptomics:

    Article Title: A transcriptomic and proteomic map of primary human cell types
    Article Snippet: .. The Human Protein Atlas (HPA) project provided a tissue-based map of the human proteome through transcriptomics and tissue microarray-based immunohistochemistry analysis [ , ]. ..

    Article Title: A transcriptomic and proteomic map of primary human cell types.
    Article Snippet: .. The Human Protein Atlas (HPA) project provided a tissue-based map of the human proteome through transcriptomics and tissue microarray-based immunohistochemistry analysis [ 4 , 5 ]. ..

    Microarray:

    Article Title: A transcriptomic and proteomic map of primary human cell types
    Article Snippet: .. The Human Protein Atlas (HPA) project provided a tissue-based map of the human proteome through transcriptomics and tissue microarray-based immunohistochemistry analysis [ , ]. ..

    Article Title: A Cytokine-Related Gene Signature for Pan-Cancer Prognostic Stratification and Malignant Phenotype Characterization
    Article Snippet: .. To examine the protein-level expression of representative cytokine-related signature genes, immunohistochemistry (IHC) analyses were performed using publicly available tissue microarray-based staining data (Human Protein Atlas). ..

    Article Title: Ferritin-nanocaged aggregation-induced emission nanoaggregates for integrated sensitive detection and treatment of gastric cancer
    Article Snippet: .. In order to verify the high expression of CD71 and Claudin18.2 in gastric cancer patients at the protein level, we mined the gastric cancer tissue microarray data in the Human protein atlas database, and confirmed that CD71 and Claudin18.2 were highly expressed in the tumor region, with a significant difference compared to the paraneoplastic expression ( D). ..

    Article Title: A Cytokine-Related Gene Signature for Pan-Cancer Prognostic Stratification and Malignant Phenotype Characterization.
    Article Snippet: .. To examine the protein-level expression of representative cytokine-related signature genes, immunohistochemistry (IHC) analyses were performed using publicly available tissue microarray-based staining data (Human Protein Atlas). ..

    Article Title: SHP2 regulates VEGFR2 Y1175/PLCγ signaling to impair tumor endothelial barrier stability
    Article Snippet: .. Human tumor tissue microarray (TMA) containing formalin-fixed paraffin-embedded core biopsies of patients with different cancer forms, generated by Human Protein Atlas (HPA) consortium, , were examined, including bladder- ( n = 12), stomach- (GAC) ( n = 12), kidney (RCC) ( n = 16) and testicular cancer ( n = 12), using immunofluorescent staining. .. Two core biopsies were collected per patient and samples were sectioned to 4 μm thickness and mounted on Superfrost Plus microscope slides (Thermo Fisher Scientific).

    Article Title: SHP2 regulates VEGFR2 Y1175/PLCγ signaling to impair tumor endothelial barrier stability
    Article Snippet: .. Human tumor tissue microarray (TMA) , Human Protein Atlas (HPA) , Uhlén et al. . ..

    Article Title: A transcriptomic and proteomic map of primary human cell types.
    Article Snippet: .. The Human Protein Atlas (HPA) project provided a tissue-based map of the human proteome through transcriptomics and tissue microarray-based immunohistochemistry analysis [ 4 , 5 ]. ..

    Article Title: Mapping cross-domain drivers of Alzheimer's disease risk through integrated network analysis.
    Article Snippet: .. In addition, we annotated each node for evidence of expression in the brain using any three different sources.Weexamined evidence of expression in normal brain tissues using the Human Protein Atlas (HPA) tissue microarray data v22.16 We assembled the list of proteins for which there was evidence (Reliability either “Enhanced”, “Supported”, or “Approved”) for any level of expression (Level either “Low”, “Medium”, “High”, “Ascending”, or “Descending”) for any of the nine brain tissues included in the normal tissue IHC data (i.e., “caudate”, “cerebellum”, “cerebral cortex”, “hippocampus”, “hypothalamus”, “pituitary gland”, “dorsal raphe”, “choroid plexus”, or “substantia nigra”). ..

    Immunohistochemistry:

    Article Title: A transcriptomic and proteomic map of primary human cell types
    Article Snippet: .. The Human Protein Atlas (HPA) project provided a tissue-based map of the human proteome through transcriptomics and tissue microarray-based immunohistochemistry analysis [ , ]. ..

    Article Title: A Cytokine-Related Gene Signature for Pan-Cancer Prognostic Stratification and Malignant Phenotype Characterization
    Article Snippet: .. To examine the protein-level expression of representative cytokine-related signature genes, immunohistochemistry (IHC) analyses were performed using publicly available tissue microarray-based staining data (Human Protein Atlas). ..

    Article Title: A Cytokine-Related Gene Signature for Pan-Cancer Prognostic Stratification and Malignant Phenotype Characterization.
    Article Snippet: .. To examine the protein-level expression of representative cytokine-related signature genes, immunohistochemistry (IHC) analyses were performed using publicly available tissue microarray-based staining data (Human Protein Atlas). ..

    Article Title: A transcriptomic and proteomic map of primary human cell types.
    Article Snippet: .. The Human Protein Atlas (HPA) project provided a tissue-based map of the human proteome through transcriptomics and tissue microarray-based immunohistochemistry analysis [ 4 , 5 ]. ..

    Article Title: Mapping cross-domain drivers of Alzheimer's disease risk through integrated network analysis.
    Article Snippet: .. In addition, we annotated each node for evidence of expression in the brain using any three different sources.Weexamined evidence of expression in normal brain tissues using the Human Protein Atlas (HPA) tissue microarray data v22.16 We assembled the list of proteins for which there was evidence (Reliability either “Enhanced”, “Supported”, or “Approved”) for any level of expression (Level either “Low”, “Medium”, “High”, “Ascending”, or “Descending”) for any of the nine brain tissues included in the normal tissue IHC data (i.e., “caudate”, “cerebellum”, “cerebral cortex”, “hippocampus”, “hypothalamus”, “pituitary gland”, “dorsal raphe”, “choroid plexus”, or “substantia nigra”). ..

    Expressing:

    Article Title: A Cytokine-Related Gene Signature for Pan-Cancer Prognostic Stratification and Malignant Phenotype Characterization
    Article Snippet: .. To examine the protein-level expression of representative cytokine-related signature genes, immunohistochemistry (IHC) analyses were performed using publicly available tissue microarray-based staining data (Human Protein Atlas). ..

    Article Title: Ferritin-nanocaged aggregation-induced emission nanoaggregates for integrated sensitive detection and treatment of gastric cancer
    Article Snippet: .. In order to verify the high expression of CD71 and Claudin18.2 in gastric cancer patients at the protein level, we mined the gastric cancer tissue microarray data in the Human protein atlas database, and confirmed that CD71 and Claudin18.2 were highly expressed in the tumor region, with a significant difference compared to the paraneoplastic expression ( D). ..

    Article Title: A Cytokine-Related Gene Signature for Pan-Cancer Prognostic Stratification and Malignant Phenotype Characterization.
    Article Snippet: .. To examine the protein-level expression of representative cytokine-related signature genes, immunohistochemistry (IHC) analyses were performed using publicly available tissue microarray-based staining data (Human Protein Atlas). ..

    Article Title: Mapping cross-domain drivers of Alzheimer's disease risk through integrated network analysis.
    Article Snippet: .. In addition, we annotated each node for evidence of expression in the brain using any three different sources.Weexamined evidence of expression in normal brain tissues using the Human Protein Atlas (HPA) tissue microarray data v22.16 We assembled the list of proteins for which there was evidence (Reliability either “Enhanced”, “Supported”, or “Approved”) for any level of expression (Level either “Low”, “Medium”, “High”, “Ascending”, or “Descending”) for any of the nine brain tissues included in the normal tissue IHC data (i.e., “caudate”, “cerebellum”, “cerebral cortex”, “hippocampus”, “hypothalamus”, “pituitary gland”, “dorsal raphe”, “choroid plexus”, or “substantia nigra”). ..

    Staining:

    Article Title: A Cytokine-Related Gene Signature for Pan-Cancer Prognostic Stratification and Malignant Phenotype Characterization
    Article Snippet: .. To examine the protein-level expression of representative cytokine-related signature genes, immunohistochemistry (IHC) analyses were performed using publicly available tissue microarray-based staining data (Human Protein Atlas). ..

    Article Title: A Cytokine-Related Gene Signature for Pan-Cancer Prognostic Stratification and Malignant Phenotype Characterization.
    Article Snippet: .. To examine the protein-level expression of representative cytokine-related signature genes, immunohistochemistry (IHC) analyses were performed using publicly available tissue microarray-based staining data (Human Protein Atlas). ..

    Article Title: SHP2 regulates VEGFR2 Y1175/PLCγ signaling to impair tumor endothelial barrier stability
    Article Snippet: .. Human tumor tissue microarray (TMA) containing formalin-fixed paraffin-embedded core biopsies of patients with different cancer forms, generated by Human Protein Atlas (HPA) consortium, , were examined, including bladder- ( n = 12), stomach- (GAC) ( n = 12), kidney (RCC) ( n = 16) and testicular cancer ( n = 12), using immunofluorescent staining. .. Two core biopsies were collected per patient and samples were sectioned to 4 μm thickness and mounted on Superfrost Plus microscope slides (Thermo Fisher Scientific).

    Formalin-fixed Paraffin-Embedded:

    Article Title: SHP2 regulates VEGFR2 Y1175/PLCγ signaling to impair tumor endothelial barrier stability
    Article Snippet: .. Human tumor tissue microarray (TMA) containing formalin-fixed paraffin-embedded core biopsies of patients with different cancer forms, generated by Human Protein Atlas (HPA) consortium, , were examined, including bladder- ( n = 12), stomach- (GAC) ( n = 12), kidney (RCC) ( n = 16) and testicular cancer ( n = 12), using immunofluorescent staining. .. Two core biopsies were collected per patient and samples were sectioned to 4 μm thickness and mounted on Superfrost Plus microscope slides (Thermo Fisher Scientific).

    Generated:

    Article Title: SHP2 regulates VEGFR2 Y1175/PLCγ signaling to impair tumor endothelial barrier stability
    Article Snippet: .. Human tumor tissue microarray (TMA) containing formalin-fixed paraffin-embedded core biopsies of patients with different cancer forms, generated by Human Protein Atlas (HPA) consortium, , were examined, including bladder- ( n = 12), stomach- (GAC) ( n = 12), kidney (RCC) ( n = 16) and testicular cancer ( n = 12), using immunofluorescent staining. .. Two core biopsies were collected per patient and samples were sectioned to 4 μm thickness and mounted on Superfrost Plus microscope slides (Thermo Fisher Scientific).



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    A. Differential CALR gene copy numbers or CALR mRNA levels between tissues derived from various cancer types and corresponding normal tissues were analysed using the Oncomine database ( p -value threshold was set at less than 0.01; all fold changes were deemed valid). Over-expression or under-expression in the top 1, 5 and 10% are color-coded according to the legend. B. Ratio of differential CRT protein levels between tissues derived from various cancer types and corresponding normal tissues were analysed using the dbDEPC 2.0 proteomics database. C. Overall CRT protein levels determined in human tumour tissues via tissue microarray analysis-based <t>immunohistochemistry</t> were retrieved using the Human Protein Atlas database. Here, the level or overall intensity of antibody-based staining has been used to generate three annotated protein expression patterns i.e. high, medium, and low, and no detection levels (colour coded here, in the legend, where the intensity of colour indicates the level of expression/staining).
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    Image Search Results


    A. Differential CALR gene copy numbers or CALR mRNA levels between tissues derived from various cancer types and corresponding normal tissues were analysed using the Oncomine database ( p -value threshold was set at less than 0.01; all fold changes were deemed valid). Over-expression or under-expression in the top 1, 5 and 10% are color-coded according to the legend. B. Ratio of differential CRT protein levels between tissues derived from various cancer types and corresponding normal tissues were analysed using the dbDEPC 2.0 proteomics database. C. Overall CRT protein levels determined in human tumour tissues via tissue microarray analysis-based immunohistochemistry were retrieved using the Human Protein Atlas database. Here, the level or overall intensity of antibody-based staining has been used to generate three annotated protein expression patterns i.e. high, medium, and low, and no detection levels (colour coded here, in the legend, where the intensity of colour indicates the level of expression/staining).

    Journal: Oncotarget

    Article Title: Resistance to anticancer vaccination effect is controlled by a cancer cell-autonomous phenotype that disrupts immunogenic phagocytic removal

    doi:

    Figure Lengend Snippet: A. Differential CALR gene copy numbers or CALR mRNA levels between tissues derived from various cancer types and corresponding normal tissues were analysed using the Oncomine database ( p -value threshold was set at less than 0.01; all fold changes were deemed valid). Over-expression or under-expression in the top 1, 5 and 10% are color-coded according to the legend. B. Ratio of differential CRT protein levels between tissues derived from various cancer types and corresponding normal tissues were analysed using the dbDEPC 2.0 proteomics database. C. Overall CRT protein levels determined in human tumour tissues via tissue microarray analysis-based immunohistochemistry were retrieved using the Human Protein Atlas database. Here, the level or overall intensity of antibody-based staining has been used to generate three annotated protein expression patterns i.e. high, medium, and low, and no detection levels (colour coded here, in the legend, where the intensity of colour indicates the level of expression/staining).

    Article Snippet: In order to increase this coverage, we decided to analyse the publicly available tissue-microarray based immunohistochemistry datasets in the Human Protein Atlas database [ ].

    Techniques: Derivative Assay, Over Expression, Expressing, Microarray, Immunohistochemistry, Staining