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rabbit anti-syntaxin3  (Synaptic Systems)


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    Structured Review

    Synaptic Systems rabbit anti-syntaxin3
    Rabbit Anti Syntaxin3, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/syntaxin3/syntaxin+2+antibody/pm38026697-52-1-6
    Average 90 stars, based on 1 article reviews
    rabbit anti-syntaxin3 - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: Method for suppressing spasmodic torticollis
    Article Snippet: Proteins were separated by SDS PAGE (12% Tris-Bis), transferred to nitrocellulose membrane and syntaxin detected using rabbit anti-syntaxin 2 antibody (Synaptic Systems, cat #110022).

    Article Title: SMAP2 Regulates Retrograde Transport from Recycling Endosomes to the Golgi
    Article Snippet: Rabbit anti-Syntaxin 5 antibody was purchased from Synaptic Systems.

    Article Title: The first synapse in vision in the aging mouse retina.
    Article Snippet: 612044); SNARE Complex: rabbit anti-Complexin4 (1:40,000, Synaptic Systems, cat.no.

    Article Title: Complement promotes endothelial von Willebrand factor and angiopoietin-2 release in obstructive sleep apnea
    Article Snippet: Immunoprecipitation and Western blotting After exposure to normoxia or IH, HUVEC lysate was incubated with antibodies for vWF (Abcam), syntaxin-3 (Synaptic Systems or Santa Cruz Biotechnology), vesicle-associated membrane protein (VAMP) 3 (Cell Signaling Technology) or VAMP8 (Novus Biologicals) overnight at 4°C.

    Article Title: Cardiac SNARE Expression in Health and Disease
    Article Snippet: Anti-VAMP1 (#104002), anti-VAMP2 (#104202), anti-VAMP3 (#43080), anti-VAMP4 (#136002), anti-VAMP5 (#176003), anti-VAMP7 (#232003), anti-VAMP8 (#104302), anti-SNAP23 (#111202), anti-SNAP29 (#111303), anti-SNAP47 (#111403), anti-syntaxin2 (#110022), anti-syntaxin3 (#110032), anti-syntaxin4 (#110042), anti-syntaxin5 (#110053), anti-syntaxin7 (#110072), anti-syntaxin8 (#110083), and anti-syntaxin16 (#110162) were from Synaptic Systems (Goettingen, Germany).

    Western Blot:

    Article Title: Method for suppressing spasmodic torticollis
    Article Snippet: Proteins were separated by SDS PAGE (12% Tris-Bis), transferred to nitrocellulose membrane and syntaxin detected using rabbit anti-syntaxin 2 antibody (Synaptic Systems, cat #110022).

    Article Title: SMAP2 Regulates Retrograde Transport from Recycling Endosomes to the Golgi
    Article Snippet: Rabbit anti-Syntaxin 5 antibody was purchased from Synaptic Systems.

    Article Title: The first synapse in vision in the aging mouse retina.
    Article Snippet: 612044); SNARE Complex: rabbit anti-Complexin4 (1:40,000, Synaptic Systems, cat.no.

    Article Title: Complement promotes endothelial von Willebrand factor and angiopoietin-2 release in obstructive sleep apnea
    Article Snippet: Immunoprecipitation and Western blotting After exposure to normoxia or IH, HUVEC lysate was incubated with antibodies for vWF (Abcam), syntaxin-3 (Synaptic Systems or Santa Cruz Biotechnology), vesicle-associated membrane protein (VAMP) 3 (Cell Signaling Technology) or VAMP8 (Novus Biologicals) overnight at 4°C.

    Article Title: Cardiac SNARE Expression in Health and Disease
    Article Snippet: Anti-VAMP1 (#104002), anti-VAMP2 (#104202), anti-VAMP3 (#43080), anti-VAMP4 (#136002), anti-VAMP5 (#176003), anti-VAMP7 (#232003), anti-VAMP8 (#104302), anti-SNAP23 (#111202), anti-SNAP29 (#111303), anti-SNAP47 (#111403), anti-syntaxin2 (#110022), anti-syntaxin3 (#110032), anti-syntaxin4 (#110042), anti-syntaxin5 (#110053), anti-syntaxin7 (#110072), anti-syntaxin8 (#110083), and anti-syntaxin16 (#110162) were from Synaptic Systems (Goettingen, Germany).

    Incubation:

    Article Title: Method for suppressing spasmodic torticollis
    Article Snippet: Proteins were separated by SDS PAGE (12% Tris-Bis), transferred to nitrocellulose membrane and syntaxin detected using rabbit anti-syntaxin 2 antibody (Synaptic Systems, cat #110022).

    Article Title: SMAP2 Regulates Retrograde Transport from Recycling Endosomes to the Golgi
    Article Snippet: Rabbit anti-Syntaxin 5 antibody was purchased from Synaptic Systems.

    Article Title: The first synapse in vision in the aging mouse retina.
    Article Snippet: 612044); SNARE Complex: rabbit anti-Complexin4 (1:40,000, Synaptic Systems, cat.no.

    Article Title: Complement promotes endothelial von Willebrand factor and angiopoietin-2 release in obstructive sleep apnea
    Article Snippet: Immunoprecipitation and Western blotting After exposure to normoxia or IH, HUVEC lysate was incubated with antibodies for vWF (Abcam), syntaxin-3 (Synaptic Systems or Santa Cruz Biotechnology), vesicle-associated membrane protein (VAMP) 3 (Cell Signaling Technology) or VAMP8 (Novus Biologicals) overnight at 4°C.

    Article Title: Cardiac SNARE Expression in Health and Disease
    Article Snippet: Anti-VAMP1 (#104002), anti-VAMP2 (#104202), anti-VAMP3 (#43080), anti-VAMP4 (#136002), anti-VAMP5 (#176003), anti-VAMP7 (#232003), anti-VAMP8 (#104302), anti-SNAP23 (#111202), anti-SNAP29 (#111303), anti-SNAP47 (#111403), anti-syntaxin2 (#110022), anti-syntaxin3 (#110032), anti-syntaxin4 (#110042), anti-syntaxin5 (#110053), anti-syntaxin7 (#110072), anti-syntaxin8 (#110083), and anti-syntaxin16 (#110162) were from Synaptic Systems (Goettingen, Germany).

    Membrane:

    Article Title: Method for suppressing spasmodic torticollis
    Article Snippet: Proteins were separated by SDS PAGE (12% Tris-Bis), transferred to nitrocellulose membrane and syntaxin detected using rabbit anti-syntaxin 2 antibody (Synaptic Systems, cat #110022).

    Article Title: SMAP2 Regulates Retrograde Transport from Recycling Endosomes to the Golgi
    Article Snippet: Rabbit anti-Syntaxin 5 antibody was purchased from Synaptic Systems.

    Article Title: The first synapse in vision in the aging mouse retina.
    Article Snippet: 612044); SNARE Complex: rabbit anti-Complexin4 (1:40,000, Synaptic Systems, cat.no.

    Article Title: Complement promotes endothelial von Willebrand factor and angiopoietin-2 release in obstructive sleep apnea
    Article Snippet: Immunoprecipitation and Western blotting After exposure to normoxia or IH, HUVEC lysate was incubated with antibodies for vWF (Abcam), syntaxin-3 (Synaptic Systems or Santa Cruz Biotechnology), vesicle-associated membrane protein (VAMP) 3 (Cell Signaling Technology) or VAMP8 (Novus Biologicals) overnight at 4°C.

    Article Title: Cardiac SNARE Expression in Health and Disease
    Article Snippet: Anti-VAMP1 (#104002), anti-VAMP2 (#104202), anti-VAMP3 (#43080), anti-VAMP4 (#136002), anti-VAMP5 (#176003), anti-VAMP7 (#232003), anti-VAMP8 (#104302), anti-SNAP23 (#111202), anti-SNAP29 (#111303), anti-SNAP47 (#111403), anti-syntaxin2 (#110022), anti-syntaxin3 (#110032), anti-syntaxin4 (#110042), anti-syntaxin5 (#110053), anti-syntaxin7 (#110072), anti-syntaxin8 (#110083), and anti-syntaxin16 (#110162) were from Synaptic Systems (Goettingen, Germany).

    Lysis:

    Article Title: Method for suppressing spasmodic torticollis
    Article Snippet: Proteins were separated by SDS PAGE (12% Tris-Bis), transferred to nitrocellulose membrane and syntaxin detected using rabbit anti-syntaxin 2 antibody (Synaptic Systems, cat #110022).

    Article Title: SMAP2 Regulates Retrograde Transport from Recycling Endosomes to the Golgi
    Article Snippet: Rabbit anti-Syntaxin 5 antibody was purchased from Synaptic Systems.

    Article Title: The first synapse in vision in the aging mouse retina.
    Article Snippet: 612044); SNARE Complex: rabbit anti-Complexin4 (1:40,000, Synaptic Systems, cat.no.

    Article Title: Complement promotes endothelial von Willebrand factor and angiopoietin-2 release in obstructive sleep apnea
    Article Snippet: Immunoprecipitation and Western blotting After exposure to normoxia or IH, HUVEC lysate was incubated with antibodies for vWF (Abcam), syntaxin-3 (Synaptic Systems or Santa Cruz Biotechnology), vesicle-associated membrane protein (VAMP) 3 (Cell Signaling Technology) or VAMP8 (Novus Biologicals) overnight at 4°C.

    Article Title: Cardiac SNARE Expression in Health and Disease
    Article Snippet: Anti-VAMP1 (#104002), anti-VAMP2 (#104202), anti-VAMP3 (#43080), anti-VAMP4 (#136002), anti-VAMP5 (#176003), anti-VAMP7 (#232003), anti-VAMP8 (#104302), anti-SNAP23 (#111202), anti-SNAP29 (#111303), anti-SNAP47 (#111403), anti-syntaxin2 (#110022), anti-syntaxin3 (#110032), anti-syntaxin4 (#110042), anti-syntaxin5 (#110053), anti-syntaxin7 (#110072), anti-syntaxin8 (#110083), and anti-syntaxin16 (#110162) were from Synaptic Systems (Goettingen, Germany).

    SDS Page:

    Article Title: Method for suppressing spasmodic torticollis
    Article Snippet: Proteins were separated by SDS PAGE (12% Tris-Bis), transferred to nitrocellulose membrane and syntaxin detected using rabbit anti-syntaxin 2 antibody (Synaptic Systems, cat #110022).

    Article Title: SMAP2 Regulates Retrograde Transport from Recycling Endosomes to the Golgi
    Article Snippet: Rabbit anti-Syntaxin 5 antibody was purchased from Synaptic Systems.

    Article Title: The first synapse in vision in the aging mouse retina.
    Article Snippet: 612044); SNARE Complex: rabbit anti-Complexin4 (1:40,000, Synaptic Systems, cat.no.

    Article Title: Complement promotes endothelial von Willebrand factor and angiopoietin-2 release in obstructive sleep apnea
    Article Snippet: Immunoprecipitation and Western blotting After exposure to normoxia or IH, HUVEC lysate was incubated with antibodies for vWF (Abcam), syntaxin-3 (Synaptic Systems or Santa Cruz Biotechnology), vesicle-associated membrane protein (VAMP) 3 (Cell Signaling Technology) or VAMP8 (Novus Biologicals) overnight at 4°C.

    Article Title: Cardiac SNARE Expression in Health and Disease
    Article Snippet: Anti-VAMP1 (#104002), anti-VAMP2 (#104202), anti-VAMP3 (#43080), anti-VAMP4 (#136002), anti-VAMP5 (#176003), anti-VAMP7 (#232003), anti-VAMP8 (#104302), anti-SNAP23 (#111202), anti-SNAP29 (#111303), anti-SNAP47 (#111403), anti-syntaxin2 (#110022), anti-syntaxin3 (#110032), anti-syntaxin4 (#110042), anti-syntaxin5 (#110053), anti-syntaxin7 (#110072), anti-syntaxin8 (#110083), and anti-syntaxin16 (#110162) were from Synaptic Systems (Goettingen, Germany).



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    Fig. 4. Effects of SKF-10047 on the protein expression of <t>Syntaxin3</t> (STX3). Syntaxin3 consists of some isoforms, produced by alternative splicing (https://www.uniprot. org/uniprot/Q13277). According to the predictive molecular size of isoforms, the isoform A and isoform B, main isoforms of STX3 were considered to be detected in COS-7 cells (left). Treatment of COS-7 cells with SKF-10047 for 48 h significantly increased the protein levels of both STX3 isoforms in a dose-dependent manner. Statistical analysis was performed for isoform B (*P < 0.05, n ¼ 3, one-way ANOVA with Dunnett's post-test).
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    Image Search Results


    Fig. 4. Effects of SKF-10047 on the protein expression of Syntaxin3 (STX3). Syntaxin3 consists of some isoforms, produced by alternative splicing (https://www.uniprot. org/uniprot/Q13277). According to the predictive molecular size of isoforms, the isoform A and isoform B, main isoforms of STX3 were considered to be detected in COS-7 cells (left). Treatment of COS-7 cells with SKF-10047 for 48 h significantly increased the protein levels of both STX3 isoforms in a dose-dependent manner. Statistical analysis was performed for isoform B (*P < 0.05, n ¼ 3, one-way ANOVA with Dunnett's post-test).

    Journal: Journal of pharmacological sciences

    Article Title: SKF-10047, a prototype Sigma-1 receptor agonist, augmented the membrane trafficking and uptake activity of the serotonin transporter and its C-terminus-deleted mutant via a Sigma-1 receptor-independent mechanism.

    doi: 10.1016/j.jphs.2018.11.005

    Figure Lengend Snippet: Fig. 4. Effects of SKF-10047 on the protein expression of Syntaxin3 (STX3). Syntaxin3 consists of some isoforms, produced by alternative splicing (https://www.uniprot. org/uniprot/Q13277). According to the predictive molecular size of isoforms, the isoform A and isoform B, main isoforms of STX3 were considered to be detected in COS-7 cells (left). Treatment of COS-7 cells with SKF-10047 for 48 h significantly increased the protein levels of both STX3 isoforms in a dose-dependent manner. Statistical analysis was performed for isoform B (*P < 0.05, n ¼ 3, one-way ANOVA with Dunnett's post-test).

    Article Snippet: An anti-Syntaxin3 rabbit polyclonal antibody was purchased from Alomone labs. (Jerusalem, Israel).

    Techniques: Expressing, Produced, Alternative Splicing

    Fig. 5. Influence of knockdown or overexpression of Syntaxin3 on the incremental effects of SKF-10047 on the SERTDCT uptake activity. We examined the effects of SKF-10047 on the SERTDCT uptake activity in STX3-knockdown or STX3-overexpressing COS-7 cells, and compared the increasing rate in these cells to that in control cells. A: Validation of STX3 knockdown by STX3 siRNA (upper) and overexpression by pMyc-DDK-STX3 (lower) in COS-7 cells. The transfection of STX3 siRNA #1 and #2 reduced the expression of STX3 isoform A, a functional isoform, by 53% and 56%, respectively (upper). In pMyc-DDK-STX3-transfected cells, prominent expression of Myc-DDK-STX3 was seen in addition to endogenous STX3, isoform A and B. B: Effects of SKF-10047 on the uptake activity of SERTDCT in control siRNA-transfected COS-7 cells and STX3 siRNA #2-transfected COS-7 cells. In both cells, 200 mM SKF-10047 significantly increased the uptake activity of SERTDCT (n ¼ 4, P < 0.001, unpaired t-test). The increase in SERTDCT uptake activity induced by 200 mM SKF-10047 was similar in control siRNA-transfected cells and STX3 siRNA-transfected cells (n ¼ 4, ns, unpaired t-test). C: Effects of SKF-10047 on the uptake activity of SERTDCT in mock-transfected COS-7 cells and Myc-DDK-STX3-expressing COS-7 cells. In both cells, 200 mM SKF-10047 significantly increased the uptake activity of SERTDCT (n ¼ 4, P < 0.001, unpaired t-test). The increase in SERTDCT uptake activity induced by 200 mM SKF-10047 in Myc-DDK-STX3-expressing cells was significantly higher than that in mock-transfected cells (n ¼ 4, P < 0.001, unpaired t-test).

    Journal: Journal of pharmacological sciences

    Article Title: SKF-10047, a prototype Sigma-1 receptor agonist, augmented the membrane trafficking and uptake activity of the serotonin transporter and its C-terminus-deleted mutant via a Sigma-1 receptor-independent mechanism.

    doi: 10.1016/j.jphs.2018.11.005

    Figure Lengend Snippet: Fig. 5. Influence of knockdown or overexpression of Syntaxin3 on the incremental effects of SKF-10047 on the SERTDCT uptake activity. We examined the effects of SKF-10047 on the SERTDCT uptake activity in STX3-knockdown or STX3-overexpressing COS-7 cells, and compared the increasing rate in these cells to that in control cells. A: Validation of STX3 knockdown by STX3 siRNA (upper) and overexpression by pMyc-DDK-STX3 (lower) in COS-7 cells. The transfection of STX3 siRNA #1 and #2 reduced the expression of STX3 isoform A, a functional isoform, by 53% and 56%, respectively (upper). In pMyc-DDK-STX3-transfected cells, prominent expression of Myc-DDK-STX3 was seen in addition to endogenous STX3, isoform A and B. B: Effects of SKF-10047 on the uptake activity of SERTDCT in control siRNA-transfected COS-7 cells and STX3 siRNA #2-transfected COS-7 cells. In both cells, 200 mM SKF-10047 significantly increased the uptake activity of SERTDCT (n ¼ 4, P < 0.001, unpaired t-test). The increase in SERTDCT uptake activity induced by 200 mM SKF-10047 was similar in control siRNA-transfected cells and STX3 siRNA-transfected cells (n ¼ 4, ns, unpaired t-test). C: Effects of SKF-10047 on the uptake activity of SERTDCT in mock-transfected COS-7 cells and Myc-DDK-STX3-expressing COS-7 cells. In both cells, 200 mM SKF-10047 significantly increased the uptake activity of SERTDCT (n ¼ 4, P < 0.001, unpaired t-test). The increase in SERTDCT uptake activity induced by 200 mM SKF-10047 in Myc-DDK-STX3-expressing cells was significantly higher than that in mock-transfected cells (n ¼ 4, P < 0.001, unpaired t-test).

    Article Snippet: An anti-Syntaxin3 rabbit polyclonal antibody was purchased from Alomone labs. (Jerusalem, Israel).

    Techniques: Knockdown, Over Expression, Activity Assay, Control, Biomarker Discovery, Transfection, Expressing, Functional Assay