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cy3 streptavidin solution  (Agilent technologies)


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    Agilent technologies cy3 streptavidin solution
    Cy3 Streptavidin Solution, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 25496 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/surescan+microarray+scanner/Streptavidin/ppr0412794-104-6-11
    Average 99 stars, based on 25496 article reviews
    cy3 streptavidin solution - by Bioz Stars, 2026-11
    99/100 stars

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    Incubation:

    Article Title: Pooled intravenous immunoglobulins exhibit broad binding and Fc-mediated activity against diverse SARS-CoV-2 variants.
    Article Snippet: .. Thirty minutes prior to washing the plates, AVI-tagged human Fc receptors (purchased from Sino Biological or Acro Biosystems) were mixed with Streptavidin-PE (ProZyme PJRS301) at a molar ratio of 1:4 (4 μM FcR: 1 μM Streptavidin-PE) and incubated by end-over end mixing for 10 min at RT. ..

    Article Title: Capybara Oil‐Based Nanoemulsion Attenuates Obesity‐Induced Renal Injury Through Modulation of Inflammation, Oxidative Stress, and Fibrosis in Mice
    Article Snippet: .. Subsequently, sections were incubated with antibodies, and these reactions were amplified using a biotin‐streptavidin system (Dako, Santa Clara, California, USA). .. Immunoreactive products were visualized using diaminobenzidine (DAB) reagent (Dako) and counterstained with hematoxylin.

    Article Title: Differences in the collagen of semitendinosus tendon in patients with and without general joint laxity
    Article Snippet: Sections were incubated overnight at 4°C with anti-human collagen type I rabbit polyclonal antibody (Abcam ab34710, UK), anti-human collagen type III goat polyclonal antibody (Southern Biotechnology Associates, Inc. #1330-01, USA), anti-human collagen type V mouse monoclonal antibody clone V-3C9 (Kyowa Pharma Chemical Co. F-61, Japan), and anti-COL12A1 rabbit polyclonal antibody (Abcam ab196619). .. The sections were then incubated with peroxidase-conjugated anti-rabbit IgG polyclonal antibody (Nichirei #424144, Japan) for collagen types I and XII, biotin-labeled anti-goat immunoglobulin rabbit antibody (DAKO E0466), peroxidase-labelled streptavidin (DAKO K0675; DAKO, Denmark) for collagen type III, and peroxidase-conjugated anti-mouse IgG polyclonal antibody (Nichirei #424134) for collagen type V. Colour development was achieved with 3,3'-diaminobenzidine-4HCl for five minutes. ..

    Article Title: Antitumor efficacy and safety profile of gallic acid in the murine sarcoma 180 model
    Article Snippet: .. Sections were incubated with primary antibodies (four sections per antibody; Supplementary Table 2) at room temperature, followed by biotinylated secondary antibody and streptavidin–HRP complex (LSAB System Kit, Dako) for 1 h. Detection was achieved with 3,3′-diaminobenzidine (Sigma), counterstained with Meyer’s hematoxylin (1 min), dehydrated, cleared in xylene, and mounted with Permount (Fisher Scientific). ..

    Article Title: Chronic Excessive Exercise Aggravates Apical Periodontitis in Mice.
    Article Snippet: The sections were incubated overnight at 4°C with primary antibodies against IL- 6 (bs- 0782R) and IL- 10 (bs- 0698R), both rabbit polyclonal antibodies (Bioss Antibodies, Woburn, MA, USA), diluted 1:200, or with a rabbit polyclonal antiTNF- α antibody (NB600- 587, Novus Biologicals, Littleton, CO, USA), diluted 1:100. .. The sections were then incubated with a biotinylated secondary antibody for 40 min, followed by incubation with the streptavidin–peroxidase complex from the EnVision FLEX/HRP detection system (K8000; Dako, Glostrup, Denmark). ..

    Article Title: Placental TRPV2 supports trophoblast function and normal fetal development.
    Article Snippet: Non-specific binding was blocked using blocking buffer (dH2O containing 2% bovine serum albumin (Sigma), 1% skimmed dry milk (Nestlé), 0.1% Tween80 (Merck) and normal goat serum (1/30), 15min, RT). .. Sections were incubated with biotinylated lectin (isolectin B4, B1205, Vector Laboratories; 1:250, 90min, 37 °C), which was detected using horseradish peroxide (HRP)-conjugated streptavidin (Agilent P0397; 1:800, 30min, RT) followed by 3’-Diaminobenzidine (Sigma, 10min, RT). ..

    Amplification:

    Article Title: Capybara Oil‐Based Nanoemulsion Attenuates Obesity‐Induced Renal Injury Through Modulation of Inflammation, Oxidative Stress, and Fibrosis in Mice
    Article Snippet: .. Subsequently, sections were incubated with antibodies, and these reactions were amplified using a biotin‐streptavidin system (Dako, Santa Clara, California, USA). .. Immunoreactive products were visualized using diaminobenzidine (DAB) reagent (Dako) and counterstained with hematoxylin.



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    Flow chart of microarray data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.

    Journal: bioRxiv

    Article Title: Synovial fluid transcriptome dynamics in osteoarthritis progression: Implications in pathogenesis

    doi: 10.1101/2024.06.24.600143

    Figure Lengend Snippet: Flow chart of microarray data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.

    Article Snippet: Following hybridization, the array slides were washed and scanned with an Agilent SureScan High-Resolution DNA Microarray Scanner.

    Techniques: Microarray, Software