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Promega superscript rt kit
Superscript Rt Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+rt/superscript+rt+kit/pmc05865483-21-30-33
Average 90 stars, based on 1 article reviews
superscript rt kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Synthesized:

Article Title: In vitro and in vivo effects of insulin-producing cells generated by xeno-antigen free 3D culture with RCP piece
Article Snippet: .. Total RNA was extracted from cells using a RNeasy Mini Kit (Qiagen, Hilden, Germany) and cDNA was synthesized from 2.5 μg of total RNA by reverse transcription using a SuperScript RT kit (Promega, Madison, WI, USA), following the manufacturer’s instructions and as previously described . qRT-PCR was performed using the Applied Biosystems 7500 real-time PCR system, TaqMan Gene Expression Assays on demand, and a TaqMan Universal Master Mix (gene-specific TaqMan probes on a StepOne Plus; Applied Biosystems, Foster City, CA, USA). .. MAFA (Hs04419852_s1) TaqMan primer was used.

Article Title: Prognostic value of CYP2W1 expression in patients with human hepatocellular carcinoma.
Article Snippet: CYP2W1 overexpression has been reported in a variety of human cancers.. However, the role of CYP2W1 in hepatocellular carcinoma (HCC) remains unclear.. This study was designed to evaluate the expression and prognostic significance of CYP2W1 in human HCC.

Article Title: A New 2-Step Acceleration Protocol Using a Histone Deacetylase Inhibitor to Generate Insulin-Producing Cells From Adipose-Derived Mesenchymal Stem Cells
Article Snippet: .. Total RNA was extracted from cells using an RNeasy Mini Kit (Qiagen, Hilden, Germany), and complementary DNA was synthesized from 2.5 μg of total RNA by reverse transcription using a SuperScript RT kit (Promega, Madison, Wis), following the manufacturer's instructions. .. Then, quantitative reverse transcription—polymerase chain reaction was performed using the Applied Biosystems 7500 real-time polymerase chain reaction system, TaqMan Gene Expression Assays on demand, and a TaqMan Universal Master Mix (gene-specific TaqMan probes on a StepOne Plus; Applied Biosystems, Foster City, Calif).

Article Title: Prognostic value of centromere protein-A expression in patients with epithelial ovarian cancer.
Article Snippet: Alteredexpressionofcentromereprotein-A(CENPA) is observed invarious types of human cancers.However, the clinical significance and pathological role of CENP-A in epithelial ovarian cancer (EOC) remains unclear.. Themain objectiveof this investigationwas toclarify the relationshipsbetween CENP-A expression and the clinicopathological features of patients with EOC.. Real-time quantitative PCR and Western blotwereperformed toexamineCENP-Aexpression in20pairs of fresh-frozen EOC tissues and corresponding noncancerous tissues.

Article Title: A New 2-Step Acceleration Protocol Using a Histone Deacetylase Inhibitor to Generate Insulin-Producing Cells From Adipose-Derived Mesenchymal Stem Cells
Article Snippet: .. We investigated the gene expression profile of the transcription factor NeuroD1 and the endocrine hormone insulin as we reported previously.18 Total RNA was extracted from cells using an RNeasy Mini Kit (Qiagen, Hilden, Germany), and complementaryDNAwas synthesized from 2.5 μg of total RNAby reverse transcription using a SuperScript RT kit (Promega, Madison, Wis), 478 www.pancreasjournal.com following the manufacturer's instructions. .. Then, quantitative reverse transcription—polymerase chain reaction was performed using the Applied Biosystems 7500 real-time polymerase chain reaction system, TaqMan Gene Expression Assays on demand, and a TaqMan Universal Master Mix (gene-specific TaqMan probes on a StepOne Plus; Applied Biosystems, Foster City, Calif ).

Article Title: Expression profile analysis of long non-coding RNAs involved in the metformin-inhibited gluconeogenesis of primary mouse hepatocytes
Article Snippet: .. Following total RNA extraction, as described above, cDNA was synthesized by reverse transcription using Superscript II RT reagent kit (Promega Corporation, Madison, WI, USA) according to the manufacturer's protocol. .. RT-qPCR was performed with the Roche LightCycle r® 480 instrument (Roche Diagnostics, Basel, Switzerland) using SYBR Premix Ex Taq (Takara Bio, Inc., Otsu, Japan) in a final volume of 10 μ l. The PCR conditions were as follows: Denaturation at 95°C for 10 sec, 40 cycles at 95°C for 5 sec and 60°C for 31 sec. A melting curve was constructed in the temperature range of 60 to 95°C at the end of the amplification.

Reverse Transcription:

Article Title: In vitro and in vivo effects of insulin-producing cells generated by xeno-antigen free 3D culture with RCP piece
Article Snippet: .. Total RNA was extracted from cells using a RNeasy Mini Kit (Qiagen, Hilden, Germany) and cDNA was synthesized from 2.5 μg of total RNA by reverse transcription using a SuperScript RT kit (Promega, Madison, WI, USA), following the manufacturer’s instructions and as previously described . qRT-PCR was performed using the Applied Biosystems 7500 real-time PCR system, TaqMan Gene Expression Assays on demand, and a TaqMan Universal Master Mix (gene-specific TaqMan probes on a StepOne Plus; Applied Biosystems, Foster City, CA, USA). .. MAFA (Hs04419852_s1) TaqMan primer was used.

Article Title: LIN28 expression and prognostic value in hepatocellular carcinoma patients who meet the Milan criteria and undergo hepatectomy
Article Snippet: RNA was treated with DNase I (Invitrogen Inc., Carlsbad, CA) to remove any DNA contamination and quantified by spectrophotometry. .. Reverse transcription was performed with the Superscript RT kit (Promega Inc., Madison, WI) according to the manufacturer's instructions, and the resultant cDNA templates were subjected to PCR amplification. ..

Article Title: A New 2-Step Acceleration Protocol Using a Histone Deacetylase Inhibitor to Generate Insulin-Producing Cells From Adipose-Derived Mesenchymal Stem Cells
Article Snippet: .. Total RNA was extracted from cells using an RNeasy Mini Kit (Qiagen, Hilden, Germany), and complementary DNA was synthesized from 2.5 μg of total RNA by reverse transcription using a SuperScript RT kit (Promega, Madison, Wis), following the manufacturer's instructions. .. Then, quantitative reverse transcription—polymerase chain reaction was performed using the Applied Biosystems 7500 real-time polymerase chain reaction system, TaqMan Gene Expression Assays on demand, and a TaqMan Universal Master Mix (gene-specific TaqMan probes on a StepOne Plus; Applied Biosystems, Foster City, Calif).

Article Title: A New 2-Step Acceleration Protocol Using a Histone Deacetylase Inhibitor to Generate Insulin-Producing Cells From Adipose-Derived Mesenchymal Stem Cells
Article Snippet: .. We investigated the gene expression profile of the transcription factor NeuroD1 and the endocrine hormone insulin as we reported previously.18 Total RNA was extracted from cells using an RNeasy Mini Kit (Qiagen, Hilden, Germany), and complementaryDNAwas synthesized from 2.5 μg of total RNAby reverse transcription using a SuperScript RT kit (Promega, Madison, Wis), 478 www.pancreasjournal.com following the manufacturer's instructions. .. Then, quantitative reverse transcription—polymerase chain reaction was performed using the Applied Biosystems 7500 real-time polymerase chain reaction system, TaqMan Gene Expression Assays on demand, and a TaqMan Universal Master Mix (gene-specific TaqMan probes on a StepOne Plus; Applied Biosystems, Foster City, Calif ).

Article Title: Expression profile analysis of long non-coding RNAs involved in the metformin-inhibited gluconeogenesis of primary mouse hepatocytes
Article Snippet: .. Following total RNA extraction, as described above, cDNA was synthesized by reverse transcription using Superscript II RT reagent kit (Promega Corporation, Madison, WI, USA) according to the manufacturer's protocol. .. RT-qPCR was performed with the Roche LightCycle r® 480 instrument (Roche Diagnostics, Basel, Switzerland) using SYBR Premix Ex Taq (Takara Bio, Inc., Otsu, Japan) in a final volume of 10 μ l. The PCR conditions were as follows: Denaturation at 95°C for 10 sec, 40 cycles at 95°C for 5 sec and 60°C for 31 sec. A melting curve was constructed in the temperature range of 60 to 95°C at the end of the amplification.

Quantitative RT-PCR:

Article Title: In vitro and in vivo effects of insulin-producing cells generated by xeno-antigen free 3D culture with RCP piece
Article Snippet: .. Total RNA was extracted from cells using a RNeasy Mini Kit (Qiagen, Hilden, Germany) and cDNA was synthesized from 2.5 μg of total RNA by reverse transcription using a SuperScript RT kit (Promega, Madison, WI, USA), following the manufacturer’s instructions and as previously described . qRT-PCR was performed using the Applied Biosystems 7500 real-time PCR system, TaqMan Gene Expression Assays on demand, and a TaqMan Universal Master Mix (gene-specific TaqMan probes on a StepOne Plus; Applied Biosystems, Foster City, CA, USA). .. MAFA (Hs04419852_s1) TaqMan primer was used.

Real-time Polymerase Chain Reaction:

Article Title: In vitro and in vivo effects of insulin-producing cells generated by xeno-antigen free 3D culture with RCP piece
Article Snippet: .. Total RNA was extracted from cells using a RNeasy Mini Kit (Qiagen, Hilden, Germany) and cDNA was synthesized from 2.5 μg of total RNA by reverse transcription using a SuperScript RT kit (Promega, Madison, WI, USA), following the manufacturer’s instructions and as previously described . qRT-PCR was performed using the Applied Biosystems 7500 real-time PCR system, TaqMan Gene Expression Assays on demand, and a TaqMan Universal Master Mix (gene-specific TaqMan probes on a StepOne Plus; Applied Biosystems, Foster City, CA, USA). .. MAFA (Hs04419852_s1) TaqMan primer was used.

Article Title: Prognostic value of centromere protein-A expression in patients with epithelial ovarian cancer.
Article Snippet: Alteredexpressionofcentromereprotein-A(CENPA) is observed invarious types of human cancers.However, the clinical significance and pathological role of CENP-A in epithelial ovarian cancer (EOC) remains unclear.. Themain objectiveof this investigationwas toclarify the relationshipsbetween CENP-A expression and the clinicopathological features of patients with EOC.. Real-time quantitative PCR and Western blotwereperformed toexamineCENP-Aexpression in20pairs of fresh-frozen EOC tissues and corresponding noncancerous tissues.

Gene Expression:

Article Title: In vitro and in vivo effects of insulin-producing cells generated by xeno-antigen free 3D culture with RCP piece
Article Snippet: .. Total RNA was extracted from cells using a RNeasy Mini Kit (Qiagen, Hilden, Germany) and cDNA was synthesized from 2.5 μg of total RNA by reverse transcription using a SuperScript RT kit (Promega, Madison, WI, USA), following the manufacturer’s instructions and as previously described . qRT-PCR was performed using the Applied Biosystems 7500 real-time PCR system, TaqMan Gene Expression Assays on demand, and a TaqMan Universal Master Mix (gene-specific TaqMan probes on a StepOne Plus; Applied Biosystems, Foster City, CA, USA). .. MAFA (Hs04419852_s1) TaqMan primer was used.

Article Title: A New 2-Step Acceleration Protocol Using a Histone Deacetylase Inhibitor to Generate Insulin-Producing Cells From Adipose-Derived Mesenchymal Stem Cells
Article Snippet: .. We investigated the gene expression profile of the transcription factor NeuroD1 and the endocrine hormone insulin as we reported previously.18 Total RNA was extracted from cells using an RNeasy Mini Kit (Qiagen, Hilden, Germany), and complementaryDNAwas synthesized from 2.5 μg of total RNAby reverse transcription using a SuperScript RT kit (Promega, Madison, Wis), 478 www.pancreasjournal.com following the manufacturer's instructions. .. Then, quantitative reverse transcription—polymerase chain reaction was performed using the Applied Biosystems 7500 real-time polymerase chain reaction system, TaqMan Gene Expression Assays on demand, and a TaqMan Universal Master Mix (gene-specific TaqMan probes on a StepOne Plus; Applied Biosystems, Foster City, Calif ).

Polymerase Chain Reaction:

Article Title: LIN28 expression and prognostic value in hepatocellular carcinoma patients who meet the Milan criteria and undergo hepatectomy
Article Snippet: RNA was treated with DNase I (Invitrogen Inc., Carlsbad, CA) to remove any DNA contamination and quantified by spectrophotometry. .. Reverse transcription was performed with the Superscript RT kit (Promega Inc., Madison, WI) according to the manufacturer's instructions, and the resultant cDNA templates were subjected to PCR amplification. ..

Amplification:

Article Title: LIN28 expression and prognostic value in hepatocellular carcinoma patients who meet the Milan criteria and undergo hepatectomy
Article Snippet: RNA was treated with DNase I (Invitrogen Inc., Carlsbad, CA) to remove any DNA contamination and quantified by spectrophotometry. .. Reverse transcription was performed with the Superscript RT kit (Promega Inc., Madison, WI) according to the manufacturer's instructions, and the resultant cDNA templates were subjected to PCR amplification. ..

RNA Extraction:

Article Title: Expression profile analysis of long non-coding RNAs involved in the metformin-inhibited gluconeogenesis of primary mouse hepatocytes
Article Snippet: .. Following total RNA extraction, as described above, cDNA was synthesized by reverse transcription using Superscript II RT reagent kit (Promega Corporation, Madison, WI, USA) according to the manufacturer's protocol. .. RT-qPCR was performed with the Roche LightCycle r® 480 instrument (Roche Diagnostics, Basel, Switzerland) using SYBR Premix Ex Taq (Takara Bio, Inc., Otsu, Japan) in a final volume of 10 μ l. The PCR conditions were as follows: Denaturation at 95°C for 10 sec, 40 cycles at 95°C for 5 sec and 60°C for 31 sec. A melting curve was constructed in the temperature range of 60 to 95°C at the end of the amplification.



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