vectastain abc kit (Vector Laboratories)
Vector Laboratories is a verified supplier 
Vector Laboratories manufactures this product 
96
Structured Review
Vector Laboratories
vectastain abc kit
Vectastain Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 11577 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/strepabcomplex/Avidin%2FBiotin+Blocking+Kit/pmc02696036-174-11-14
Average 96 stars, based on 11577 article reviews
Vectastain Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 11577 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/strepabcomplex/Avidin%2FBiotin+Blocking+Kit/pmc02696036-174-11-14
Average 96 stars, based on 11577 article reviews
vectastain abc kit - by Bioz Stars,
2026-11
96/100 stars
Images
1) Product Images from "CCHCR1 Is Up-Regulated in Skin Cancer and Associated with EGFR Expression"
Article Title: CCHCR1 Is Up-Regulated in Skin Cancer and Associated with EGFR Expression
Journal: PLoS ONE
doi: 10.1371/journal.pone.0006030
Figure Legend Snippet: Serial sections of grade I SCC (A–C) and normal skin (H–J) were immunostained with antibodies against CCHCR1, Ki67, and EGFR. Higher magnifications of A–C are also shown (D–F, respectively). CCHCR1 staining (G) in an adjacent section to EGFR staining (F). CCHCR1 protein (A) is expressed in proliferative cancer cells at the invasive front of dermal cancer cell islands of an SCC in association with the hyperproliferation marker Ki67 (B) and EGFR (C). The Ki67 positive cells (E) express CCHCR1 (D). EGFR staining (F) associates with CCHCR1 staining (G) in adjacent sections. Normal skin samples express CCHCR1 (H) and EGFR (J) in basal KCs, while Ki67 expression (I) is more sparse. Arrows point at illustrative positions. Scale bars: (A–C) 50 µm; (D–G) 12.5 µm; (H–J) 25 µm.
Techniques Used: Staining, Marker, Expressing
Figure Legend Snippet: Serial sections of grade III (A, B) and grade II (C, D) SCC were stained with the indicated antibodies. Higher magnification of another grade II SCC (E, F). In grade III SCC (A, B), expression of CCHCR1 is heterogeneous: many, but not all, cancer cells express both CCHCR1 (A) and Ki67 (B). The insets show lower magnification of the same region, asterisk points out a cancer cell with negative CCHCR1 and Ki67 expression. In grade II SCC (C, D), infiltrative outgrowth is CCHCR1 positive (C), and the CCHCR1 positive cancer nests are also cyclin-D1 positive (D). Adjacent sections of grade II SCC (E, F) show association of CCHCR1 and cyclin-D1 positive cells. Arrows indicate corresponding areas. Scale bars: (A,B,E,F) 12.5 µm; (C,D, insets) 50 µm.
Techniques Used: Staining, Expressing
Figure Legend Snippet: Serial sections of BCC (A, B) were stained with antibodies against CCHCR1 and EGFR. Higher magnifications of A and B are also shown (C, D, respectively). CCHCR1 protein is expressed in a granular pattern in the cytoplasm of the palisading cancer cells of a nodular BCC (A, C). CCHCR1 expression (C) co-localizes with EGFR staining (D). CCHCR1 and EGFR are also expressed in basal KCs (A, B). Arrows show corresponding positions. Scale bars: (A,B) 50 µm; (C,D) 25 µm.
Techniques Used: Staining, Expressing
Figure Legend Snippet: CCHCR1 transfected HaCaT cells (A–D) stained with antibodies against CCHCR1 (A) and EGFR (B). Overlay of A and B is shown in (C), and DAPI staining showing nuclei in (D). Cells expressing CCHCR1 (green) were also positive for EGFR (red) but the two proteins did not co-localize (C). CCHCR1 positive (arrow) and negative (arrow head) cells exhibit similar EGFR expression suggesting that CCHCR1 overexpression does not affect EGFR expression (B).
Techniques Used: Transfection, Staining, Expressing, Over Expression
Figure Legend Snippet: Serial sections of KA (A, B, E, F) were stained with the antibodies against CCHCR1 and EGFR (A, B) or against CCHCR1 and Ki67 (E, F). Higher magnifications of A and B are shown (C, D, respectively). The pushing border of a KA is CCHCR1 positive (A) co-localizing with EGFR (B). Another sample is shown (E) with a prominent lymphocyte infiltrate and cytoplasmic CCHCR1 expression of the pushing border cells. More scarce Ki67 expression (F) is defined to the same areas but not necessarily to the same cells. Arrows indicate corresponding positions. Scale bars: (A, B, E, F) 50 µm; (C, D) 12.5 µm.
Techniques Used: Staining, Expressing
Figure Legend Snippet: Sections of Bowen's disease (A–D) immunostained with antibodies against CCHCR1 and EGFR as indicated. Serial sections of psoriasis (E, F) were stained with antibodies for CCHCR1 and Ki67. AK samples (G, H) were stained with CCHCR1 antibodies. In Bowen's disease (A, B), spongiosis, inflammatory infiltrate, and capillary proliferation are associated with CCHCR1 expression while areas lacking inflammation and spongiosis express less CCHCR1 (C). EGFR expression (D) in basal and suprabasal KCs of dermal papillae associates with CCHCR1 expression (B). Also in psoriasis (E), CCHCR1 is expressed basally or suprabasally (arrows) overlying the dermal papillae (dp) while rete ridges (rr) projecting into the dermis are almost negative for CCHCR1. In contrast, Ki67 expression (F) localizes to regions that are almost negative for CCHCR1 cells (arrowheads). AK samples (G) express CCHCR1 basally and suprabasally in association with KC heterogeneity, spongiosis or inflammation but adjacent areas with less inflammation or spongiosis (H) stain only faintly. Scale bars: (A–D) 50 µm; (E, F) 25 µm; (G, H) 12.5 µm.
Techniques Used: Staining, Expressing
Figure Legend Snippet: A) Ki67, EGFR, cyclin-D1 and CCHCR1 gene expression profile of five normal epidermal KC and eight cutaneous SCC cell lines (heatmap). Signal values of the probe sets were compared to the mean signal values of each probe set in KCs. The colouring is based on the log2 values of the change in the signal values. The up-regulated genes are shown in red and down-regulated genes are shown in green. B) Correlation between CCHCR1 and Ki67 probe sets was calculated between the signal values of one CCHCR1 and one Ki67 probe set in the HG-U133 Plus 3.0 array. Pearson's correlation coefficient R = 0.88. C) CCHCR1 and D) Ki67 mRNA expression levels in the normal KCs and cutaneous SCC cell lines as measured by qRT-PCR (TaqMan). Expression levels of CCHCR1 and Ki67 in the normal KCs and SCC cell lines were analyzed by qRT-PCR and corrected for the β-actin mRNA levels in the same samples. E) Correlation between CCHCR1 and Ki67 probe sets was calculated between the signal values of CCHCR1 and Ki67 mRNA levels. Pearson's correlation coefficient R = 0.46.
Techniques Used: Expressing, Quantitative RT-PCR
Figure Legend Snippet: CCHCR1 (A), Ki67 (B), and EGFR (C) mRNA expression levels (TaqMan) in HaCaT cells after treatment with the tumor promoters OA and menadione. CCHCR1 (D), Ki67 (E) and EGFR (F) mRNA expression in HaCaT, A5, II4, RT3, A431, and FaDu cells. The invasive cells II4 and FaDu and metastatic RT3 cells expressed less CCHCR1 (D) and Ki67 (E) than HaCaT and A5 cells. A431 cells (F) express EGFR mRNA clearly more than other cell lines. The ras-transformed clones A5, II4, and RT3 and FaDu cells express EGFR less than HaCaT cells. Quantitative RT-PCR results are shown relative to mRNA levels from corresponding control cells (assigned the value 1). Expression levels of CCHCR1, Ki67, and EGFR in HaCaT cells were normalized to the GAPDH mRNA levels in the same samples. * p<0.05, ** p<0.01, *** p<0.001.
Techniques Used: Expressing, Transformation Assay, Clone Assay, Quantitative RT-PCR
Figure Legend Snippet: A) Relative expression of CCHCR1 mRNA (as measured by TaqMan) at different time points (from 24 h to 8 d) after releasing the cells from serum starvation and high density culturing. Expression of CCHCR1 did not differ between confluent (control) cells and quiescent (starved) cells. Control cells expressed CCHCR1 2.5-fold more than proliferative HaCaT cells (24–48 h, 3–4 d). As confluency was reattained (8 d), the expression of CCHCR1 increased to the level of the control cells. B) Correlation between relative CCHCR1 and Ki67 expression levels. When CCHCR1 mRNA expression levels were compared to those of Ki67, a negative correlation was seen, confirming the proliferative status of the HaCaT cells. C) EGFR mRNA expression correlated with CCHCR1 mRNA expression. D) Correlation between CCHCR1 and Ki67 expression in the experiment with lower cell density. The negative correlation of CCHCR1 expression with Ki67 expression was even more profound as relative CCHCR1 mRNA decreased near to zero in the two control cells. E) Correlation between CCHCR1 and EGFR expression in the experiment with lower cell density. Here again, CCHCR1 expression correlated with EGFR expression. TaqMan PCR results are shown relative to mRNA levels from corresponding control cells assigned the value 1. Expression levels of CCHCR1, Ki67, and EGFR in HaCaT cells were normalized to the GAPDH mRNA levels in the same samples. * p<0.05, ** p<0.01, *** p<0.001.
Techniques Used: Expressing
Related Articles
Incubation:Article Title: Precursor Cells in the Parenchyma Act in Concert with Ventricular Neural Progenitors to Facilitate Dramatic Astrocyte Turnover and Recovery Following Natural Neuronal Death Article Snippet: .. Slides were then incubated in a 0.03% hydrogen peroxide and subsequently an Article Title: FXR-YAP signalling maintains biliary epithelial cell identity and preserves liver homeostasis. Article Snippet: Bile acids (BAs) flow through ducts lined by biliary epithelial cells (BECs), which preserve ductal integrity and liver homeostasis.. Failure of this barrier causes BA accumulation in the liver parenchyma, and ultimately fibrosis.. Here we show that BECs safeguard biliary barrier integrity and restrain BA-induced fibrogenesis through a cell-intrinsic mechanism involving farnesoid-X-receptor (FXR)–YAP signalling. Article Title: IL-12-producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy. Article Snippet: Please cite this article as: Yamamoto M, Zhang R, Kobayashi T, Li Y, Xu H, Mashima H, Saito K, Kariya M, Ide M, Hagiya Y, Fukuda E, Uemura S, Osako Y, Nakaki R, Nakatsura T, Fukushima S, Kaneko S, Hotta A, Ohdan H, Uemura.. Y, IL-12–producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy, Molecular Therapy (2026), doi: https:// doi.org/10.1016/j.ymthe.2026.10.017. Avidin-Biotin Assay:Article Title: Precursor Cells in the Parenchyma Act in Concert with Ventricular Neural Progenitors to Facilitate Dramatic Astrocyte Turnover and Recovery Following Natural Neuronal Death Article Snippet: .. Slides were then incubated in a 0.03% hydrogen peroxide and subsequently an Article Title: Effects of Glial Cell Line-Derived Neurotrophic Factor and Ciliary Neurotrophic Factor on Extraocular and Limb Muscle Precursor Cells. Article Snippet: Skeletal muscle has the capacity to regenerate after injury or disease,1 and this regenerative capacity is due to populations of myogenic precursor cells found within the muscles.2,3 More recently, it has been shown that normal adult skeletal muscles have a slow rate of myonuclear turnover throughout life, occurring at apparently random locations along the length of individual myofibers.4,5 This process is even more striking in the extraocular muscles, which show a high rate of myonuclear turnover throughout life in the absence of injury or skeletal muscle-related diseases.4,6,7 Based on our studies and those of other laboratories, this may be due, in part, to the presence of two distinct populations of myogenic precursor cells within the extraocular muscles.. In addition to the PAX7positive satellite cells found in all skeletal muscles,8 we 1435666 JHCXXX10.1369/00221554261435666CNTF, GDNF and EOM Precursor CellsWinker et al. research-article2026 Article Title: FXR-YAP signalling maintains biliary epithelial cell identity and preserves liver homeostasis. Article Snippet: Bile acids (BAs) flow through ducts lined by biliary epithelial cells (BECs), which preserve ductal integrity and liver homeostasis.. Failure of this barrier causes BA accumulation in the liver parenchyma, and ultimately fibrosis.. Here we show that BECs safeguard biliary barrier integrity and restrain BA-induced fibrogenesis through a cell-intrinsic mechanism involving farnesoid-X-receptor (FXR)–YAP signalling. Article Title: RIPK3 acts as a molecular switch between neuroprotection and neuropathology during herpes simplex encephalitis Article Snippet: Following an additional three washes in TBS-TX, sections were mounted with ProLong Gold Antifade Reagent containing DAPI (Cat# P36930, Thermo Fisher Scientific) and imaged using a Nikon confocal microscope. .. Phosphorylated MLKL (p-MLKL; Nature Communications Article in Press Ser345) was detected following publishedprocedures; briefly, sections were subjected to sequential blocking steps per the manufacturer’s instructions to minimize background: nonspecific antibody binding (Vector Laboratories, Cat#SP-5035-100), and Article Title: IL-12-producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy. Article Snippet: Please cite this article as: Yamamoto M, Zhang R, Kobayashi T, Li Y, Xu H, Mashima H, Saito K, Kariya M, Ide M, Hagiya Y, Fukuda E, Uemura S, Osako Y, Nakaki R, Nakatsura T, Fukushima S, Kaneko S, Hotta A, Ohdan H, Uemura.. Y, IL-12–producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy, Molecular Therapy (2026), doi: https:// doi.org/10.1016/j.ymthe.2026.10.017. Blocking Assay:Article Title: Precursor Cells in the Parenchyma Act in Concert with Ventricular Neural Progenitors to Facilitate Dramatic Astrocyte Turnover and Recovery Following Natural Neuronal Death Article Snippet: .. Slides were then incubated in a 0.03% hydrogen peroxide and subsequently an Article Title: Effects of Glial Cell Line-Derived Neurotrophic Factor and Ciliary Neurotrophic Factor on Extraocular and Limb Muscle Precursor Cells. Article Snippet: Skeletal muscle has the capacity to regenerate after injury or disease,1 and this regenerative capacity is due to populations of myogenic precursor cells found within the muscles.2,3 More recently, it has been shown that normal adult skeletal muscles have a slow rate of myonuclear turnover throughout life, occurring at apparently random locations along the length of individual myofibers.4,5 This process is even more striking in the extraocular muscles, which show a high rate of myonuclear turnover throughout life in the absence of injury or skeletal muscle-related diseases.4,6,7 Based on our studies and those of other laboratories, this may be due, in part, to the presence of two distinct populations of myogenic precursor cells within the extraocular muscles.. In addition to the PAX7positive satellite cells found in all skeletal muscles,8 we 1435666 JHCXXX10.1369/00221554261435666CNTF, GDNF and EOM Precursor CellsWinker et al. research-article2026 Article Title: FXR-YAP signalling maintains biliary epithelial cell identity and preserves liver homeostasis. Article Snippet: Bile acids (BAs) flow through ducts lined by biliary epithelial cells (BECs), which preserve ductal integrity and liver homeostasis.. Failure of this barrier causes BA accumulation in the liver parenchyma, and ultimately fibrosis.. Here we show that BECs safeguard biliary barrier integrity and restrain BA-induced fibrogenesis through a cell-intrinsic mechanism involving farnesoid-X-receptor (FXR)–YAP signalling. Article Title: RIPK3 acts as a molecular switch between neuroprotection and neuropathology during herpes simplex encephalitis Article Snippet: Following an additional three washes in TBS-TX, sections were mounted with ProLong Gold Antifade Reagent containing DAPI (Cat# P36930, Thermo Fisher Scientific) and imaged using a Nikon confocal microscope. .. Phosphorylated MLKL (p-MLKL; Nature Communications Article in Press Ser345) was detected following publishedprocedures; briefly, sections were subjected to sequential blocking steps per the manufacturer’s instructions to minimize background: nonspecific antibody binding (Vector Laboratories, Cat#SP-5035-100), and Article Title: IL-12-producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy. Article Snippet: Please cite this article as: Yamamoto M, Zhang R, Kobayashi T, Li Y, Xu H, Mashima H, Saito K, Kariya M, Ide M, Hagiya Y, Fukuda E, Uemura S, Osako Y, Nakaki R, Nakatsura T, Fukushima S, Kaneko S, Hotta A, Ohdan H, Uemura.. Y, IL-12–producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy, Molecular Therapy (2026), doi: https:// doi.org/10.1016/j.ymthe.2026.10.017. Cell Culture:Article Title: Effects of Glial Cell Line-Derived Neurotrophic Factor and Ciliary Neurotrophic Factor on Extraocular and Limb Muscle Precursor Cells. Article Snippet: Skeletal muscle has the capacity to regenerate after injury or disease,1 and this regenerative capacity is due to populations of myogenic precursor cells found within the muscles.2,3 More recently, it has been shown that normal adult skeletal muscles have a slow rate of myonuclear turnover throughout life, occurring at apparently random locations along the length of individual myofibers.4,5 This process is even more striking in the extraocular muscles, which show a high rate of myonuclear turnover throughout life in the absence of injury or skeletal muscle-related diseases.4,6,7 Based on our studies and those of other laboratories, this may be due, in part, to the presence of two distinct populations of myogenic precursor cells within the extraocular muscles.. In addition to the PAX7positive satellite cells found in all skeletal muscles,8 we 1435666 JHCXXX10.1369/00221554261435666CNTF, GDNF and EOM Precursor CellsWinker et al. research-article2026 Amplification:Article Title: FXR-YAP signalling maintains biliary epithelial cell identity and preserves liver homeostasis. Article Snippet: Bile acids (BAs) flow through ducts lined by biliary epithelial cells (BECs), which preserve ductal integrity and liver homeostasis.. Failure of this barrier causes BA accumulation in the liver parenchyma, and ultimately fibrosis.. Here we show that BECs safeguard biliary barrier integrity and restrain BA-induced fibrogenesis through a cell-intrinsic mechanism involving farnesoid-X-receptor (FXR)–YAP signalling. Binding Assay:Article Title: RIPK3 acts as a molecular switch between neuroprotection and neuropathology during herpes simplex encephalitis Article Snippet: Following an additional three washes in TBS-TX, sections were mounted with ProLong Gold Antifade Reagent containing DAPI (Cat# P36930, Thermo Fisher Scientific) and imaged using a Nikon confocal microscope. .. Phosphorylated MLKL (p-MLKL; Nature Communications Article in Press Ser345) was detected following publishedprocedures; briefly, sections were subjected to sequential blocking steps per the manufacturer’s instructions to minimize background: nonspecific antibody binding (Vector Laboratories, Cat#SP-5035-100), and Transcranial Magnetic Stimulation:Article Title: IL-12-producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy. Article Snippet: Please cite this article as: Yamamoto M, Zhang R, Kobayashi T, Li Y, Xu H, Mashima H, Saito K, Kariya M, Ide M, Hagiya Y, Fukuda E, Uemura S, Osako Y, Nakaki R, Nakatsura T, Fukushima S, Kaneko S, Hotta A, Ohdan H, Uemura.. Y, IL-12–producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy, Molecular Therapy (2026), doi: https:// doi.org/10.1016/j.ymthe.2026.10.017. |