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Promega first-strand buffer
First Strand Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/strand+buffer/first+strand+buffer/pmc04411717-103-17-19
Average 90 stars, based on 1 article reviews
first-strand buffer - by Bioz Stars, 2026-10
90/100 stars

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Reverse Transcription:

Article Title: Histone methylase MLL1 coordinates with HIF and regulate lncRNA HOTAIR expression under hypoxia.
Article Snippet: Accepted Manuscript Histone methylase MLL1 coordinates with HIF and regulate lncRNA HOTAIR expression under hypoxia Arunoday Bhan, Paromita Deb, Nadine Shihabeddin, Khairul I. Ansari, Marco Brotto, Subhrangsu S. Mandal PII: S0378-1119(17)30604-2 DOI: doi: 10.1016/j.gene.2017.07.069 Reference: GENE 42093 To appear in: Gene Received date: 21 April 2017 Revised date: 11 July 2017 Accepted date: 25 July 2017 Please cite this article as: Arunoday Bhan, Paromita Deb, Nadine Shihabeddin, Khairul I. Ansari, Marco Brotto, Subhrangsu S. Mandal , Histone methylase MLL1 coordinates with HIF and regulate lncRNA HOTAIR expression under hypoxia, Gene (2017), doi: 10.1016/ j.gene.2017.07.069 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Modified FGF-21 polypeptides comprising an internal deletion and uses thereof
Article Snippet: For quantitative PCR, Total RNA was extracted from liver samples using RNAiso (Takara Bio Inc., Japan) according to the manufacturer's instructions. .. One μg of RNA was reverse-transcribed using a reaction mixture containing 4.4 mM MgCl2 (F. Hoffmann-La Roche Ltd., Switzerland), 40 U RNase inhibitor (Toyobo Co., Ltd., Japan), 0.5 mM dNTP (Promega Corporation, USA), 6.28 μM random hexamer (Promega Corporation), 5× first strand buffer (Promega), 10 mM dithiothreitol (Invitrogen) and 200 U MMLV-RT (Invitrogen) in a final volume of 20 μL. .. The reaction was carried out for 1 hour at 37° C., followed by 5 minutes at 99° C. Real-time PCR was performed using real-time PCR DICE and SYBR premix Taq (Takara Bio).

Article Title: The IGSF1, Wnt5a, FGF14 , and ITPR1 Gene Expression and Prognosis Hallmark of Prostate Cancer
Article Snippet: The optical density (OD) of each sample at 260 and 280 nm (measured using Ultrospec 2100, Biochrom, USA) was used as criteria to determine the amount of each sample. .. Reverse transcription of RNA was performed at 37 °C for 1 hour in a 20 μl reaction mixture containing: first strand buffer, 200 units of Moloney murine leukemia virus reverse transcriptase, 20 units of RNasin, 10 mM DTT, 4.75 μM random hexamers, and 500 μM deoxynucleotides (all from Promega, Madison, WI). ..

Article Title: Initiation of Conceptus Elongation Coincides with an Endometrium Basic Fibroblast Growth Factor (FGF2) Protein Increase in Heifers
Article Snippet: The RNA amount was spectrophotometrically determined at 260 nm by a Nanodrop 1000 ND-1000 (peqLab Biotechnologie GmbH, Erlangen, Germany). .. One μg total RNA was reverse transcribed to cDNA in a reaction of 50 μM Random Hexamer Primers (Invitrogen GmbH, Karlsruhe, Germany), 10 mM dNTPs (Fermentas GmbH, St. Leon-Roth, Germany), 5× First Strand Buffer (Promega, Madison, USA) and 200 U M-MLV RT H(-) Enzym (Promega, Wisconsin, USA). ..

other:

Article Title: Intrinsic terminators in Mycoplasma hyopneumoniae transcription
Article Snippet: The mixture was heated to 70°C for 5 min and then incubated on ice for 5 min. First-strand buffer (Promega, USA), 0.1 M dithiothreitol, 40 U RNase inhibitor (Promega, USA) and 200 U M-MLV RT (Moloney Murine Leukemia Virus Reverse Transcriptase—Promega, USA) were then added to a total volume of 20 μl.

Article Title: Complex comprising RNAi molecule and N-acetylated chitosan
Article Snippet: TABLE 2 Evaluation criteria of the intestinal epithelial damage and inflammatory infiltration Index Intestinal epithelial damage Inflammatory infiltration 0 Normal No infiltration 1 Loss of goblet cells Infiltration around crypt base 2 Loss of goblet cells Infiltration reaching conjunctival in larger area muscle plate 3 Loss of crypts Extensive infiltration reaching conjunctival muscle plate and mucosal thickening with many edemas 4 Loss of crypts in larger Infiltration under mucosal area membrane (Quantitative RT-PCR) Total RNA was extracted from colon samples with RNAiso (Takara Bio Inc., Japan) in accordance with manufacturer's instructions.

Isolation:

Article Title: Uremic solutes modulate hepatic bile acid handling and induce mitochondrial toxicity.
Article Snippet: Next, efflux rates were normalized to the amount of cellular protein present in the samples as determined by a Biorad protein assay (Biorad). .. RNA was isolated from primary human hepatocytes exposed to 500 μM of uremic solutes or DMSO using the RNeasy® mini kit (Qiagen) following incubation at 37 °C and 5% (v/v) CO2 for 72 h. Subsequently, RNA was converted into cDNA using 300 ng of RNA, 0.625mM dNTPs (Life Technologies), 0.25 μg/μL random primers (Thermo Fisher Scientific), 10mM DTT (Promega), 20 U RNAsin (Promega), and 5% (v/ v) M-MLV (Thermo Fisher Scientific) in a total volume of 20 μL firststrand buffer solution (Promega), which was incubated at 20 °C for 10min, 42 °C for 45min, and 95 °C for 10min. .. Then, 2.5 μL of the resulting cDNA was mixed with 1.25 μL of primer-probe set (Applied Biosystems) for the transporter of interest, 12.5 μL Taqman mastermix (Applied Biosystems), and 8.75 μL of sterile MQ.

Incubation:

Article Title: Uremic solutes modulate hepatic bile acid handling and induce mitochondrial toxicity.
Article Snippet: Next, efflux rates were normalized to the amount of cellular protein present in the samples as determined by a Biorad protein assay (Biorad). .. RNA was isolated from primary human hepatocytes exposed to 500 μM of uremic solutes or DMSO using the RNeasy® mini kit (Qiagen) following incubation at 37 °C and 5% (v/v) CO2 for 72 h. Subsequently, RNA was converted into cDNA using 300 ng of RNA, 0.625mM dNTPs (Life Technologies), 0.25 μg/μL random primers (Thermo Fisher Scientific), 10mM DTT (Promega), 20 U RNAsin (Promega), and 5% (v/ v) M-MLV (Thermo Fisher Scientific) in a total volume of 20 μL firststrand buffer solution (Promega), which was incubated at 20 °C for 10min, 42 °C for 45min, and 95 °C for 10min. .. Then, 2.5 μL of the resulting cDNA was mixed with 1.25 μL of primer-probe set (Applied Biosystems) for the transporter of interest, 12.5 μL Taqman mastermix (Applied Biosystems), and 8.75 μL of sterile MQ.

Random Hexamer:

Article Title: Modified FGF-21 polypeptides comprising an internal deletion and uses thereof
Article Snippet: For quantitative PCR, Total RNA was extracted from liver samples using RNAiso (Takara Bio Inc., Japan) according to the manufacturer's instructions. .. One μg of RNA was reverse-transcribed using a reaction mixture containing 4.4 mM MgCl2 (F. Hoffmann-La Roche Ltd., Switzerland), 40 U RNase inhibitor (Toyobo Co., Ltd., Japan), 0.5 mM dNTP (Promega Corporation, USA), 6.28 μM random hexamer (Promega Corporation), 5× first strand buffer (Promega), 10 mM dithiothreitol (Invitrogen) and 200 U MMLV-RT (Invitrogen) in a final volume of 20 μL. .. The reaction was carried out for 1 hour at 37° C., followed by 5 minutes at 99° C. Real-time PCR was performed using real-time PCR DICE and SYBR premix Taq (Takara Bio).

Article Title: Initiation of Conceptus Elongation Coincides with an Endometrium Basic Fibroblast Growth Factor (FGF2) Protein Increase in Heifers
Article Snippet: The RNA amount was spectrophotometrically determined at 260 nm by a Nanodrop 1000 ND-1000 (peqLab Biotechnologie GmbH, Erlangen, Germany). .. One μg total RNA was reverse transcribed to cDNA in a reaction of 50 μM Random Hexamer Primers (Invitrogen GmbH, Karlsruhe, Germany), 10 mM dNTPs (Fermentas GmbH, St. Leon-Roth, Germany), 5× First Strand Buffer (Promega, Madison, USA) and 200 U M-MLV RT H(-) Enzym (Promega, Wisconsin, USA). ..

Virus:

Article Title: The IGSF1, Wnt5a, FGF14 , and ITPR1 Gene Expression and Prognosis Hallmark of Prostate Cancer
Article Snippet: The optical density (OD) of each sample at 260 and 280 nm (measured using Ultrospec 2100, Biochrom, USA) was used as criteria to determine the amount of each sample. .. Reverse transcription of RNA was performed at 37 °C for 1 hour in a 20 μl reaction mixture containing: first strand buffer, 200 units of Moloney murine leukemia virus reverse transcriptase, 20 units of RNasin, 10 mM DTT, 4.75 μM random hexamers, and 500 μM deoxynucleotides (all from Promega, Madison, WI). ..



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Image Search Results


Journal: STAR Protocols

Article Title: Protocol to isolate broadly neutralizing monoclonal antibodies against SARS-CoV-2 from human B cells

doi: 10.1016/j.xpro.2025.104275

Figure Lengend Snippet: RT mix-II

Article Snippet: 5× First-Strand Buffer (part of the SuperScript III Reverse Transcriptase) , Thermo Fisher Scientific , Cat#18080044.

Techniques: