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Promega storage buffer
Storage Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/storage+buffer/storage+buffer+b/pmc04063647-42-6-8
Average 90 stars, based on 1 article reviews
storage buffer - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Oligodendrocyte precursor cells express a functional chemokine receptor CCR3: implications for myelination.
Article Snippet: Myelination in the central nervous system requires an accurate interplay between oligodendrocyte precursor cells (OPC) and axons.. By as yet not fully understood mechanisms, OPC proliferate, migrate to the axon to be myelinated and finally differentiate into mature oligodendrocytes.. The recent finding that OPC express CXC chemokine receptors led us to the investigation of the expression and functional importance of CC chemokine receptors.

Article Title: An in vitro method to assess toxicity of waterborne metals to fish.
Article Snippet: Article history: Received 8 November 2007 Received 1 February 2008 Accepted 6 February 2008 Available online 26 February 2008 The transcription of metal-responsive genes in the rainbow trout (Oncorhynchus mykiss) gill tissue can be used to detect effects of bioreactive metals in natural waters.. Here we take advantage of an in vitro gill epithelium, which can be directly exposed to test water samples.. The in vitro gill epithelial model mimics the molecular response of in vivo gill epithelial cells to waterborne contaminants.

Article Title: Influence of culture conditions on metal-induced responses in a cultured rainbow trout gill epithelium.
Article Snippet: A primary culture technique for rainbow trout (Oncorhynchus mykiss) gill cells was optimized to better represent the intact gill in vivo in response to waterborne toxic metals.. Modifications in cell seeding density and culture conditions resulted in a gill epithelial cell culture model, which displayed classic in vivo responses to toxic metals.. Metallothionein-A (MTA), metallothionein-B (MTB), zinc transporter-1 (ZnT-1), glutathione-S-transferase (GST), and glucose-6-phosphate-dehydrogenase (G6PD) all showed dose-dependent increases in expression at the mRNA level in response to waterborne zinc.

Recombinant:

Article Title: Heme oxygenase-1 stabilizes the blood-spinal cord barrier and limits oxidative stress and white matter damage in the acutely injured murine spinal cord.
Article Snippet: .. Briefly, recombinant luciferase was diluted to 1 mg/mL in luciferase storage buffer (Promega, Madison, WI, USA) and stored at 701C. ..

Luciferase:

Article Title: Heme oxygenase-1 stabilizes the blood-spinal cord barrier and limits oxidative stress and white matter damage in the acutely injured murine spinal cord.
Article Snippet: .. Briefly, recombinant luciferase was diluted to 1 mg/mL in luciferase storage buffer (Promega, Madison, WI, USA) and stored at 701C. ..

Purification:

Article Title: Bone marrow mesenchymal stem cell-derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non-canonical Wnt signaling pathway
Article Snippet: .. The vectors were dialyzed in a storage buffer (Promega, Madison, WI, USA) following purification using a cesium chloride gradient (Sigma-Aldrich, Shanghai, China), followed by transfection into HEK293 cells via lipofectamine 2000 (Invitrogen Life Technologies) for 24 h. Subsequently the titers of the adenovirus were determined. ..

Article Title: Bone marrow mesenchymal stem cell-derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non-canonical Wnt signaling pathway.
Article Snippet: .. The vectors were dialyzed in a storage buffer (Promega, Madison, WI, USA) following purification using a cesium chloride gradient (Sigma‐Aldrich, Shanghai, China), followed by transfection into HEK293 cells via lipofectamine 2000 (Invitrogen Life Technologies) for 24 h. Subsequently the titers of the adenovirus were determined. ..

Transfection:

Article Title: Bone marrow mesenchymal stem cell-derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non-canonical Wnt signaling pathway
Article Snippet: .. The vectors were dialyzed in a storage buffer (Promega, Madison, WI, USA) following purification using a cesium chloride gradient (Sigma-Aldrich, Shanghai, China), followed by transfection into HEK293 cells via lipofectamine 2000 (Invitrogen Life Technologies) for 24 h. Subsequently the titers of the adenovirus were determined. ..

Article Title: Bone marrow mesenchymal stem cell-derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non-canonical Wnt signaling pathway.
Article Snippet: .. The vectors were dialyzed in a storage buffer (Promega, Madison, WI, USA) following purification using a cesium chloride gradient (Sigma‐Aldrich, Shanghai, China), followed by transfection into HEK293 cells via lipofectamine 2000 (Invitrogen Life Technologies) for 24 h. Subsequently the titers of the adenovirus were determined. ..

other:

Article Title: Bone morphogenetic protein-2 used in spinal fusion with spinal cord injury penetrates intrathecally and elicits a functional signaling cascade.
Article Snippet: BACKGROUND CONTEXT: The use of re FDA device/drug Author disclosures research program of of Defense).. The views express reflect the official p Defense, or US Gove 1529-9430/10/$ – see doi:10.1016/j.spinee.2 combinant human bone morphogenetic protein-2 (rhBMP-2) and its indications for spinal fusion continue to be expanded with recent reports citing spinal trauma application.. However, there are no data establishing the effects of rhBMP-2 on the injured spinal cord.

Amplification:

Article Title: Reconstruction of fig wasp mating structure: how many mothers share a fig?
Article Snippet: Fig wasps (Hymenoptera: Agaonidae) represent an important model system for studies of sex ratio evolution, mainly because they may adjust their sex ratios in response to the numbers of ovipositing females (foundresses) that enter a fig and their clutch size.. Until recently, it was assumed that all foundresses fail to re-emerge from the figs that they have entered to oviposit, but there is increasing evidence that such re-emergence may be routine.. The common practice of counting the number of dead foundresses present in a fig in order to deduce the number of foundresses is therefore questionable in species where failure to re-emerge has not been confirmed.



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Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

Journal: STAR Protocols

Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system

doi: 10.1016/j.xpro.2026.104471

Figure Lengend Snippet: Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

Article Snippet: Note: We use commercial flow cytometry staining buffer from eBioscience (Cat# 00-4222-26), which is PBS-based formulation designed to prevent non-specific antibody binding and maintain cell stability during flow cytometry.

Techniques: Flow Cytometry, Isolation, Staining