stemdiff tm astrocyte differentiation kit #100-0013 (STEMCELL Technologies Inc)

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STEMCELL Technologies Inc stemdiff tm astrocyte differentiation kit #100-0013

Characterisation of ‘healthy’ iPSC‐derived astrocytes. (a) Representative images showing differentiation and ICC staining of iPSC‐derived astrocytic cells at Day 45+. The astrocytes were differentiated from ‘healthy’ control NPCs for >40 days using astrocytes differentiation and <t>maturation</t> protocols. Top) Phase contrast images of control astrocytes on Days 2, 10 and 40 in culture post differentiation. Bottom) Cells were stained using immunofluorescent antibodies for astrocytic markers ALDH1L1 (left, green), S100β (middle, green) and GFAP (right, red), nuclei were counterstained in each image with DAPI (blue). Scale bars: 100 μM. (b) Cellular glycogen content of control astrocytes following exposure to hypoglycaemic conditions and treatment with DAB over 60 and 120 min. (c) Glycogen content of cells treated with dbcAMP an isoproterenol. (d) Glycogen contents of cells treated with Glutamate, oubain, glutamate and ouabain, TBOA and glutamate and TBOA for 60, 180 and 360 min. Results are expressed as ug/mg protein ± SD, n = 3 p < 0.05 (*), p < 0.01 (**), p < 0.001 (***). For DAB (b), a two‐way analysis of variance (ANOVA) was performed follow by Sidaks post‐test. Comparisons between treatments (c) were performed using analysis of variance (ANOVA) followed by Dunnet's post‐test. Each replicate or ‘ n ’ represents an independent culture preparation and is displayed as an individual data point.

Stemdiff Tm Astrocyte Differentiation Kit #100 0013, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stemdiff+co+kit/stemdiff+astrocyte+maturation+medium/pmc11655965-47-38-47
Average 90 stars, based on 1 article reviews

stemdiff tm astrocyte differentiation kit #100-0013 - by Bioz Stars, 2026-10

90/100 stars

Images

1) Product Images from "Altered metabolic function induced by Aβ‐oligomers and PSEN1 mutations in iPSC ‐derived astrocytes"

Article Title: Altered metabolic function induced by Aβ‐oligomers and PSEN1 mutations in iPSC ‐derived astrocytes

Journal: Journal of Neurochemistry

doi: 10.1111/jnc.16267

Figure Legend Snippet: Characterisation of ‘healthy’ iPSC‐derived astrocytes. (a) Representative images showing differentiation and ICC staining of iPSC‐derived astrocytic cells at Day 45+. The astrocytes were differentiated from ‘healthy’ control NPCs for >40 days using astrocytes differentiation and maturation protocols. Top) Phase contrast images of control astrocytes on Days 2, 10 and 40 in culture post differentiation. Bottom) Cells were stained using immunofluorescent antibodies for astrocytic markers ALDH1L1 (left, green), S100β (middle, green) and GFAP (right, red), nuclei were counterstained in each image with DAPI (blue). Scale bars: 100 μM. (b) Cellular glycogen content of control astrocytes following exposure to hypoglycaemic conditions and treatment with DAB over 60 and 120 min. (c) Glycogen content of cells treated with dbcAMP an isoproterenol. (d) Glycogen contents of cells treated with Glutamate, oubain, glutamate and ouabain, TBOA and glutamate and TBOA for 60, 180 and 360 min. Results are expressed as ug/mg protein ± SD, n = 3 p < 0.05 (*), p < 0.01 (**), p < 0.001 (***). For DAB (b), a two‐way analysis of variance (ANOVA) was performed follow by Sidaks post‐test. Comparisons between treatments (c) were performed using analysis of variance (ANOVA) followed by Dunnet's post‐test. Each replicate or ‘ n ’ represents an independent culture preparation and is displayed as an individual data point.

Techniques Used: Derivative Assay, Staining, Control

other:

Article Title: Altered metabolic function induced by Aβ-oligomers and PSEN1 mutations in iPSC-derived astrocytes.
Article Snippet: Cells were plated at 5 × 104 /cm2 density and maintained in a 37°C, 5% CO2/ 95% air atmosphere with a total medium exchange every other day, through two subsequent passages, before switching to astrocyte maturation medium (STEMdifftm Astrocyte maturation kit #100- 0016, StemCell Technologies, Cambridge, UK).

Article Title: Generation and Characterization of Human iPSC-Derived Astrocytes with Potential for Modeling X-Linked Adrenoleukodystrophy Phenotypes
Article Snippet: The NPCs were differentiated with a STEMdiff TM Astrocyte differentiation kit and STEMdiff TM Astrocyte maturation kit (STEMCELL Technologies) as per the manufacturer’s protocol.

Article Title: Altered metabolic function induced by Aβ-oligomers and PSEN1 mutations in iPSC-derived astrocytes.
Article Snippet: After 24 h cells were washed with D- PBS before medium was exchanged for astrocyte differentiation medium (ADM; STEMdifftm Astrocyte differentiation kit #100- 0013, StemCell Technologies, Cambridge, UK) and a full media exchange was completed every day for 7 days.

Activity Assay:

Article Title: The Alzheimer's disease-associated complement receptor 1 variant confers risk by impacting glial phagocytosis.
Article Snippet: .. Further studies are needed to delineate how enhanced glial phagocytic capacity contributes to neurodegeneration, with the potential to uncover novel therapeutic strategies aimed at fine-tuning glial phagocytic activity in Alzheimer’s and related disorders. were differentiated intomicroglia using the embryoid body (EB) protocol as previously described,16,20 or into astrocytes using the STEMdiff Astrocyte differentiation kit and the STEMdiff Astrocyte maturation kit using themanufacturer’s EB protocol (STEMCELL Technologies). .. iPSC-microglia and astrocytes grown on black optically clear flatbottom tissue culture-treated 384-well PhenoPlates (PerkinElmer) or black μclear CELLSTAR tissue culture-treated 96-well plates (Greiner Bio-One Ltd) were fixed (4% paraformaldehyde), washed in PBS, incubated in blocking buffer [PBS, 3% bovine serum albumin (BSA), 2% normal goat serum; plus 0.1% Triton X-100 for intracellular staining], then with primary antibodies at optimal dilutions in blocking buffer overnight.

Cell Culture:

Article Title: FABP7 Expression Modulates the Response of Astrocytes to Induced Endotoxemia
Article Snippet: .. Induced NPCs were cultured for 3 weeks in astrocyte differentiation media (STEMdiff Astrocyte Differentiation Media, Stemcell), followed by 3 weeks in astrocyte maturation media (STEMdiff Astrocyte Maturation Media, Stemcell). .. Astrocyte differentiation was confirmed by assessing GFAP , S100B , and ALDH1L1 gene expression, as previously shown (Hamilton et al. ).

Article Title: The Alzheimer's disease-associated complement receptor 1 variant confers risk by impacting glial phagocytosis.
Article Snippet: Myeloid precursors were seeded at a density of 7 × 103 cells per well (50 μL/well) inmicroglia differentiationmedia in black optical 384-well plates (PerkinElmer) and allowed to differentiate into microglia over a period of 10 days. .. The iPSC-derived astrocytes cultured in STEMdiff AstrocyteMaturationmedium (STEMCELL Technologies) for 20–30 days were seeded at a density of 7 × 103 cells per well (50 μL/well). .. On the day of the phagocytosis assay, some wells were treated with the cytoskeleton disruptor Cytochalasin D (CytoD; 10 μM, Sigma) for 1 h to disable phagocytosis (negative controls).

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