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digital micrograph software  (Gatan Inc)


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    Gatan Inc digital micrograph software
    Digital Micrograph Software, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 97/100, based on 12074 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sted+microscopy+data/DigitalMicrograph+Software/pmc03273009-196-11-14
    Average 97 stars, based on 12074 article reviews
    digital micrograph software - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Microscopy:

    Article Title: Alloy exsolution in co-doped PrBaMn2-xTMxO5+δ (TM = Co and/or Ni) obtained by mechanochemistry
    Article Snippet: were performed by combining HAADF and EDX acquisitions in STEM mode and were accomplished with the VELOX software (FEI Europe). HR micrograph analysis, fast Fourier transform (FFT) and phase interpretation were done with the Gatan Digital Micrograph software (Gatan Inc.) and the Java version of the electron microscope software (JEMS). The electrode polarization resistance in air and hydrogen atmospheres was determined in symmetrical cell configur

    Software:

    Article Title: Alloy exsolution in co-doped PrBaMn2-xTMxO5+δ (TM = Co and/or Ni) obtained by mechanochemistry
    Article Snippet: were performed by combining HAADF and EDX acquisitions in STEM mode and were accomplished with the VELOX software (FEI Europe). HR micrograph analysis, fast Fourier transform (FFT) and phase interpretation were done with the Gatan Digital Micrograph software (Gatan Inc.) and the Java version of the electron microscope software (JEMS). The electrode polarization resistance in air and hydrogen atmospheres was determined in symmetrical cell configur

    Electrochemiluminescence:

    Article Title: Alloy exsolution in co-doped PrBaMn2-xTMxO5+δ (TM = Co and/or Ni) obtained by mechanochemistry
    Article Snippet: were performed by combining HAADF and EDX acquisitions in STEM mode and were accomplished with the VELOX software (FEI Europe). HR micrograph analysis, fast Fourier transform (FFT) and phase interpretation were done with the Gatan Digital Micrograph software (Gatan Inc.) and the Java version of the electron microscope software (JEMS). The electrode polarization resistance in air and hydrogen atmospheres was determined in symmetrical cell configur

    Transmission Assay:

    Article Title: Alloy exsolution in co-doped PrBaMn2-xTMxO5+δ (TM = Co and/or Ni) obtained by mechanochemistry
    Article Snippet: were performed by combining HAADF and EDX acquisitions in STEM mode and were accomplished with the VELOX software (FEI Europe). HR micrograph analysis, fast Fourier transform (FFT) and phase interpretation were done with the Gatan Digital Micrograph software (Gatan Inc.) and the Java version of the electron microscope software (JEMS). The electrode polarization resistance in air and hydrogen atmospheres was determined in symmetrical cell configur

    Transmission Electron Microscopy:

    Article Title: Alloy exsolution in co-doped PrBaMn2-xTMxO5+δ (TM = Co and/or Ni) obtained by mechanochemistry
    Article Snippet: were performed by combining HAADF and EDX acquisitions in STEM mode and were accomplished with the VELOX software (FEI Europe). HR micrograph analysis, fast Fourier transform (FFT) and phase interpretation were done with the Gatan Digital Micrograph software (Gatan Inc.) and the Java version of the electron microscope software (JEMS). The electrode polarization resistance in air and hydrogen atmospheres was determined in symmetrical cell configur

    Electron Microscopy:

    Article Title: Alloy exsolution in co-doped PrBaMn2-xTMxO5+δ (TM = Co and/or Ni) obtained by mechanochemistry
    Article Snippet: were performed by combining HAADF and EDX acquisitions in STEM mode and were accomplished with the VELOX software (FEI Europe). HR micrograph analysis, fast Fourier transform (FFT) and phase interpretation were done with the Gatan Digital Micrograph software (Gatan Inc.) and the Java version of the electron microscope software (JEMS). The electrode polarization resistance in air and hydrogen atmospheres was determined in symmetrical cell configur

    Centrifugation:

    Article Title: Alloy exsolution in co-doped PrBaMn2-xTMxO5+δ (TM = Co and/or Ni) obtained by mechanochemistry
    Article Snippet: were performed by combining HAADF and EDX acquisitions in STEM mode and were accomplished with the VELOX software (FEI Europe). HR micrograph analysis, fast Fourier transform (FFT) and phase interpretation were done with the Gatan Digital Micrograph software (Gatan Inc.) and the Java version of the electron microscope software (JEMS). The electrode polarization resistance in air and hydrogen atmospheres was determined in symmetrical cell configur

    Staining:

    Article Title: Alloy exsolution in co-doped PrBaMn2-xTMxO5+δ (TM = Co and/or Ni) obtained by mechanochemistry
    Article Snippet: were performed by combining HAADF and EDX acquisitions in STEM mode and were accomplished with the VELOX software (FEI Europe). HR micrograph analysis, fast Fourier transform (FFT) and phase interpretation were done with the Gatan Digital Micrograph software (Gatan Inc.) and the Java version of the electron microscope software (JEMS). The electrode polarization resistance in air and hydrogen atmospheres was determined in symmetrical cell configur

    Concentration Assay:

    Article Title: Alloy exsolution in co-doped PrBaMn2-xTMxO5+δ (TM = Co and/or Ni) obtained by mechanochemistry
    Article Snippet: were performed by combining HAADF and EDX acquisitions in STEM mode and were accomplished with the VELOX software (FEI Europe). HR micrograph analysis, fast Fourier transform (FFT) and phase interpretation were done with the Gatan Digital Micrograph software (Gatan Inc.) and the Java version of the electron microscope software (JEMS). The electrode polarization resistance in air and hydrogen atmospheres was determined in symmetrical cell configur

    Imaging:

    Article Title: Alloy exsolution in co-doped PrBaMn2-xTMxO5+δ (TM = Co and/or Ni) obtained by mechanochemistry
    Article Snippet: were performed by combining HAADF and EDX acquisitions in STEM mode and were accomplished with the VELOX software (FEI Europe). HR micrograph analysis, fast Fourier transform (FFT) and phase interpretation were done with the Gatan Digital Micrograph software (Gatan Inc.) and the Java version of the electron microscope software (JEMS). The electrode polarization resistance in air and hydrogen atmospheres was determined in symmetrical cell configur

    Cell Culture:

    Article Title: Alloy exsolution in co-doped PrBaMn2-xTMxO5+δ (TM = Co and/or Ni) obtained by mechanochemistry
    Article Snippet: were performed by combining HAADF and EDX acquisitions in STEM mode and were accomplished with the VELOX software (FEI Europe). HR micrograph analysis, fast Fourier transform (FFT) and phase interpretation were done with the Gatan Digital Micrograph software (Gatan Inc.) and the Java version of the electron microscope software (JEMS). The electrode polarization resistance in air and hydrogen atmospheres was determined in symmetrical cell configur

    Produced:

    Article Title: Alloy exsolution in co-doped PrBaMn2-xTMxO5+δ (TM = Co and/or Ni) obtained by mechanochemistry
    Article Snippet: were performed by combining HAADF and EDX acquisitions in STEM mode and were accomplished with the VELOX software (FEI Europe). HR micrograph analysis, fast Fourier transform (FFT) and phase interpretation were done with the Gatan Digital Micrograph software (Gatan Inc.) and the Java version of the electron microscope software (JEMS). The electrode polarization resistance in air and hydrogen atmospheres was determined in symmetrical cell configur

    Control:

    Article Title: Alloy exsolution in co-doped PrBaMn2-xTMxO5+δ (TM = Co and/or Ni) obtained by mechanochemistry
    Article Snippet: were performed by combining HAADF and EDX acquisitions in STEM mode and were accomplished with the VELOX software (FEI Europe). HR micrograph analysis, fast Fourier transform (FFT) and phase interpretation were done with the Gatan Digital Micrograph software (Gatan Inc.) and the Java version of the electron microscope software (JEMS). The electrode polarization resistance in air and hydrogen atmospheres was determined in symmetrical cell configur



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    Increases in mitochondrial mass in response to activation through the BCR and/or TLR9. a) Purified B cells were stimulated with CpG (1 μM) and/or anti-IgM (5 μg/ml) or left unstimulated for 24h and stained with Live/Dead marker, fixed, permeabilized and stained with antibodies specific for the mitochondrial markers: TOM20; COXIV; and VDAC1. MFI levels quantified by flow cytometry are shown. Data represent three independent experiments each carried out with three replicates. Bars represent mean. b,c) B cells cultured in vitro for 24 h as in (a) were stained with Live/Dead marker and viable B cells were FACS sorted and lysates from equal number of cells were analyzed by immunoblot for: Hsp60; Sirt3, TOM20 and actin. The immunoblot was cropped guided by the appropriate molecular weight markers. Representative immunoblot (b) and quantification of the blot relative to actin (c) are given. Data represent three independent experiments. d,e) Purified mouse splenic B cells were cultured in vitro for 24 h in media containing CpG (1 μM) and/or anti-IgM (5 μg/ml), stained with Live/Dead marker and MitoTracker Red, washed and plated on poly-L-lysine coated coverslips. Cells were fixed, permeabilized and stained with TOM20-specific antibodies and DAPI and imaged by <t>STED</t> <t>microscopy.</t> Representative microscope images (d) and quantification of mitochondrial volume for each stimulation condition (e) are shown. Scale bar = 2 μm. Data represent two independent experiments. Images of at least 30 viable cells per experiment were analyzed for each condition. Dashed lines represent the mean values. f,g) Purified B cells cultured in vitro for 24 h as in (a) were harvested and total DNA and RNA were isolated. The relative levels of COI DNA to 18S DNA (f) and TFAM gene expressions compared to unstimulated condition were quantified by qPCR (g). Data represent three independent experiments each of which were carried out in triplicates. Bars represent mean. ( P >0.05 = n.s.; 0.01 < P ≤0.05 = *;0.001 < P ≤0.01 = **; 0.0001 <P ≤0.001 = ***; P ≤0.0001= ****) (Panels a,f,g: One Way ANOVA with Tukey’s adjustment; Panel e: Mann-Withney test:).
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    Image Search Results


    Increases in mitochondrial mass in response to activation through the BCR and/or TLR9. a) Purified B cells were stimulated with CpG (1 μM) and/or anti-IgM (5 μg/ml) or left unstimulated for 24h and stained with Live/Dead marker, fixed, permeabilized and stained with antibodies specific for the mitochondrial markers: TOM20; COXIV; and VDAC1. MFI levels quantified by flow cytometry are shown. Data represent three independent experiments each carried out with three replicates. Bars represent mean. b,c) B cells cultured in vitro for 24 h as in (a) were stained with Live/Dead marker and viable B cells were FACS sorted and lysates from equal number of cells were analyzed by immunoblot for: Hsp60; Sirt3, TOM20 and actin. The immunoblot was cropped guided by the appropriate molecular weight markers. Representative immunoblot (b) and quantification of the blot relative to actin (c) are given. Data represent three independent experiments. d,e) Purified mouse splenic B cells were cultured in vitro for 24 h in media containing CpG (1 μM) and/or anti-IgM (5 μg/ml), stained with Live/Dead marker and MitoTracker Red, washed and plated on poly-L-lysine coated coverslips. Cells were fixed, permeabilized and stained with TOM20-specific antibodies and DAPI and imaged by STED microscopy. Representative microscope images (d) and quantification of mitochondrial volume for each stimulation condition (e) are shown. Scale bar = 2 μm. Data represent two independent experiments. Images of at least 30 viable cells per experiment were analyzed for each condition. Dashed lines represent the mean values. f,g) Purified B cells cultured in vitro for 24 h as in (a) were harvested and total DNA and RNA were isolated. The relative levels of COI DNA to 18S DNA (f) and TFAM gene expressions compared to unstimulated condition were quantified by qPCR (g). Data represent three independent experiments each of which were carried out in triplicates. Bars represent mean. ( P >0.05 = n.s.; 0.01 < P ≤0.05 = *;0.001 < P ≤0.01 = **; 0.0001 <P ≤0.001 = ***; P ≤0.0001= ****) (Panels a,f,g: One Way ANOVA with Tukey’s adjustment; Panel e: Mann-Withney test:).

    Journal: Nature immunology

    Article Title: Second signals rescue B cells from activation-induced mitochondrial dysfunction and death

    doi: 10.1038/s41590-018-0156-5

    Figure Lengend Snippet: Increases in mitochondrial mass in response to activation through the BCR and/or TLR9. a) Purified B cells were stimulated with CpG (1 μM) and/or anti-IgM (5 μg/ml) or left unstimulated for 24h and stained with Live/Dead marker, fixed, permeabilized and stained with antibodies specific for the mitochondrial markers: TOM20; COXIV; and VDAC1. MFI levels quantified by flow cytometry are shown. Data represent three independent experiments each carried out with three replicates. Bars represent mean. b,c) B cells cultured in vitro for 24 h as in (a) were stained with Live/Dead marker and viable B cells were FACS sorted and lysates from equal number of cells were analyzed by immunoblot for: Hsp60; Sirt3, TOM20 and actin. The immunoblot was cropped guided by the appropriate molecular weight markers. Representative immunoblot (b) and quantification of the blot relative to actin (c) are given. Data represent three independent experiments. d,e) Purified mouse splenic B cells were cultured in vitro for 24 h in media containing CpG (1 μM) and/or anti-IgM (5 μg/ml), stained with Live/Dead marker and MitoTracker Red, washed and plated on poly-L-lysine coated coverslips. Cells were fixed, permeabilized and stained with TOM20-specific antibodies and DAPI and imaged by STED microscopy. Representative microscope images (d) and quantification of mitochondrial volume for each stimulation condition (e) are shown. Scale bar = 2 μm. Data represent two independent experiments. Images of at least 30 viable cells per experiment were analyzed for each condition. Dashed lines represent the mean values. f,g) Purified B cells cultured in vitro for 24 h as in (a) were harvested and total DNA and RNA were isolated. The relative levels of COI DNA to 18S DNA (f) and TFAM gene expressions compared to unstimulated condition were quantified by qPCR (g). Data represent three independent experiments each of which were carried out in triplicates. Bars represent mean. ( P >0.05 = n.s.; 0.01 < P ≤0.05 = *;0.001 < P ≤0.01 = **; 0.0001

    Article Snippet: Confocal and STED microscopy data were analyzed using Imaris (Bitplane).

    Techniques: Activation Assay, Purification, Staining, Marker, Flow Cytometry, Cell Culture, In Vitro, Western Blot, Molecular Weight, Microscopy, Isolation