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nuclear stain dapi  (Promega)

 
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    Structured Review

    Promega nuclear stain dapi
    Nuclear Stain Dapi, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+with+dapi/dapi+staining/pm18728402-228-1-7
    Average 90 stars, based on 1 article reviews
    nuclear stain dapi - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Nm23-H1 modulates the activity of the guanine exchange factor Dbl-1.
    Article Snippet: Rabbit anti-Dbl antibody was purchased from Santa Cruz (Santa Cruz, CA), anti-Nm23 antibody was obtained from SEIKAGAKU Corp. (Tokyo, Japan), anti-Myc antibody 9E10 and anti-HA antibody 12CA5, were culture products of hybridoma cell lines. .. The nuclear stain DAPI was purchased from Promega (Madison, WI). ..

    Article Title: Use of oligosaccharide compounds for the prevention and treatment of pathological scars
    Article Snippet: .. The immunolabeling is carried out using DAPI staining (which gives a blue color) for the cell nuclei, a rabbit anti-α-SMA antibody and an anti-rabbit secondary antibody coupled to Cys3 (sold by Promega). ..

    Article Title: Id-1 promotes TGF-beta1-induced cell motility through HSP27 activation and disassembly of adherens junction in prostate epithelial cells.
    Article Snippet: Article Chronology: Received 27 March 2007 Revised version received 17 August 2007 Accepted 23 August 2007 Available online 5 September 2007 Id-1 (inhibitor of differentiation or DNA binding-1) has been positively associated with cell proliferation, cell cycle progression, and invasiveness during tumorigenesis.. In addition, Id1 has been shown tomodulate cellular sensitivity to TGF-β1 (transforming growth factor β1).. Here we demonstrate a novel role of Id-1 in promoting TGF-β1-induced cell motility in a non-malignant prostate epithelial cell line, NPTX.

    Article Title: The suppressor of metastasis Nm23-H1 interacts with the Cdc42 Rho family member and the pleckstrin homology domain of oncoprotein Dbl-1 to suppress cell migration.
    Article Snippet: Rabbit anti-Dbl antibody was purchased from Santa Cruz (Santa Cruz, CA), anti-Nm23-H1 antibody was obtained from Seikagaku Corp (Tokyo, Japan), anti-Myc antibody 9E10 and anti-HA antibody 12CA5, were obtained from hybridoma cell culture. .. The nuclear stain DAPI was purchased from Promega Inc.(Madison, WI). ..

    Immunolabeling:

    Article Title: Use of oligosaccharide compounds for the prevention and treatment of pathological scars
    Article Snippet: .. The immunolabeling is carried out using DAPI staining (which gives a blue color) for the cell nuclei, a rabbit anti-α-SMA antibody and an anti-rabbit secondary antibody coupled to Cys3 (sold by Promega). ..

    other:

    Article Title: Sonazoid-Conjugated Natural Killer Cells for Tumor Therapy and Real-Time Visualization by Ultrasound Imaging
    Article Snippet: The results were evaluated by DAPI (Promega, Madison, WI, USA) staining and analyzed with a flow cytometer (MACSQuant, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Control:

    Article Title: Id-1 promotes TGF-beta1-induced cell motility through HSP27 activation and disassembly of adherens junction in prostate epithelial cells.
    Article Snippet: Article Chronology: Received 27 March 2007 Revised version received 17 August 2007 Accepted 23 August 2007 Available online 5 September 2007 Id-1 (inhibitor of differentiation or DNA binding-1) has been positively associated with cell proliferation, cell cycle progression, and invasiveness during tumorigenesis.. In addition, Id1 has been shown tomodulate cellular sensitivity to TGF-β1 (transforming growth factor β1).. Here we demonstrate a novel role of Id-1 in promoting TGF-β1-induced cell motility in a non-malignant prostate epithelial cell line, NPTX.

    Western Blot:

    Article Title: Id-1 promotes TGF-beta1-induced cell motility through HSP27 activation and disassembly of adherens junction in prostate epithelial cells.
    Article Snippet: Article Chronology: Received 27 March 2007 Revised version received 17 August 2007 Accepted 23 August 2007 Available online 5 September 2007 Id-1 (inhibitor of differentiation or DNA binding-1) has been positively associated with cell proliferation, cell cycle progression, and invasiveness during tumorigenesis.. In addition, Id1 has been shown tomodulate cellular sensitivity to TGF-β1 (transforming growth factor β1).. Here we demonstrate a novel role of Id-1 in promoting TGF-β1-induced cell motility in a non-malignant prostate epithelial cell line, NPTX.

    Transfection:

    Article Title: Id-1 promotes TGF-beta1-induced cell motility through HSP27 activation and disassembly of adherens junction in prostate epithelial cells.
    Article Snippet: Article Chronology: Received 27 March 2007 Revised version received 17 August 2007 Accepted 23 August 2007 Available online 5 September 2007 Id-1 (inhibitor of differentiation or DNA binding-1) has been positively associated with cell proliferation, cell cycle progression, and invasiveness during tumorigenesis.. In addition, Id1 has been shown tomodulate cellular sensitivity to TGF-β1 (transforming growth factor β1).. Here we demonstrate a novel role of Id-1 in promoting TGF-β1-induced cell motility in a non-malignant prostate epithelial cell line, NPTX.



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    Image Search Results


    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing