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pbs wash  (Biosynth Carbosynth)


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    Biosynth Carbosynth pbs wash
    Pbs Wash, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 96/100, based on 440 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sigma+plot+12%2E5+computer+software/PBS/pmc08973849-314-29-15
    Average 96 stars, based on 440 article reviews
    pbs wash - by Bioz Stars, 2026-11
    96/100 stars

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    Incubation:

    Article Title: Orthotopic model for the analysis of melanoma circulating tumor cells
    Article Snippet: .. The frozen sections were permeabilized with 0.1% Triton X-100 in PBS, blocked with a 1% solution of bovine serum albumin in PBS, and then incubated overnight at 4 °C with primary antibody against GFP (20R-GR-011, Fitzgerald Industries International, Acton, MA) diluted in 1% BSA in PBS. .. Subsequently, the sections were incubated with a secondary antibody, Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (1:500, A-11034, Invitrogen, Carlsbad, CA), for 1 h at room temperature.

    Article Title: Citrullinated isomer of myelin basic protein can induce inflammatory responses in astrocytes.
    Article Snippet: .. After 21 days, the astrocytes— cells remaining adherent after the removal of microglia—were removed from the flasks by incubation with 0.25% trypsin (Carbosynth, Compton Berkshire, UK) for 10 min. After being washed in PBS, cells were ready for seeding and experimental use. ..

    Article Title: Type I Interferon, Induced by Adenovirus or Adenoviral Vector Infection, Regulates the Cytokine Response to Lipopolysaccharide in a Macrophage Type-Specific Manner
    Article Snippet: .. Frozen spleens were sectioned into 8 μm-thick sections using a Leica cryostat, fixed with acetone at 0°C for 5 min, and rehydrated with PBS for 30 min. To block endogenous peroxidase, sections were treated with Peroxo-Block (Invitrogen) for 1 min, permeabilized in PBS containing 5% normal donkey serum and 0.1% Triton X-100 for 20 min, and then incubated with rabbit anti-luciferase antibody (Fitzgerald, 70C-CR2020RAP) in PBS containing 3% normal donkey serum at 4°C overnight. .. For detection of luciferase, signal amplification with the TSA fluorescein system (Perkin-Elmer) was performed according to the manufacturer’s instructions using a biotin-conjugated donkey anti-rabbit antibody (Jackson ImmunoResearch).

    Article Title: Next Generation Chemiluminescent Probes for Antimalarial Drug Discovery
    Article Snippet: Luminescence stability was quantified using a theoretical parasite per well range of 20 000–1, 3-fold diluted, and lysed in H 2 O (20 μL per well of a 384-well plate Greiner Bio-One, cat. #781086). .. 20 000 F12 wt parasites were used as negative control. β-gal SENSOR probe (Biosynth AG, cat. #A-8169_P00) was dissolved in PBS containing 1 mM MgCl 2 to a concentration of 20 μM, incubated at RT for 30–60 min and subsequently added to each well to a final concentration of 10 μM. .. Chemiluminescence was measured every 15 min (0–180 min) using PerkinElmer’s IVIS Spectrum In Vivo Imaging System Lumina LT Series III at RT.

    Article Title: Type I interferon, induced by adenovirus or adenoviral vector infection, regulates the cytokine response to LPS in a macrophage type-specific manner.
    Article Snippet: .. Frozen spleens were sectioned into 8 μm-thick sections using a Leica cryostat, fixed with acetone at 0°C for 5 min and rehydrated with PBS for 30 min. To block endogenous peroxidase sections were treated with Peroxo-Block (Invitrogen) for 1 min, permeabilized in PBS containing 5% normal donkey serum and 0.1% Triton X-100 for 20 min., and then incubated with rabbit antiluciferase antibody (Fitzgerald, 70C-CR2020RAP) in PBS containing 3% normal donkey serum at 4°C overnight. .. For detection of luciferase, signal amplification with the TSA fluorescein system (Perkin-Elmer) was performed according to the manufacturer's instructions using a biotin-conjugated donkey anti-rabbit antibody (Jackson ImmunoResearch).

    Article Title: The modulatory effects of large and small extracellular vesicles from normal human urine on calcium oxalate crystallization, growth, aggregation, adhesion on renal cells, and invasion through extracellular matrix: An in vitro study.
    Article Snippet: .. The crystal complexes were then incubated with 200 μl of 0.3 pM Lys-plasminogen (Fitzgerald Industries International; Acton, MA) in PBS at 37◦C for 60 min. ..

    Blocking Assay:

    Article Title: Type I Interferon, Induced by Adenovirus or Adenoviral Vector Infection, Regulates the Cytokine Response to Lipopolysaccharide in a Macrophage Type-Specific Manner
    Article Snippet: .. Frozen spleens were sectioned into 8 μm-thick sections using a Leica cryostat, fixed with acetone at 0°C for 5 min, and rehydrated with PBS for 30 min. To block endogenous peroxidase, sections were treated with Peroxo-Block (Invitrogen) for 1 min, permeabilized in PBS containing 5% normal donkey serum and 0.1% Triton X-100 for 20 min, and then incubated with rabbit anti-luciferase antibody (Fitzgerald, 70C-CR2020RAP) in PBS containing 3% normal donkey serum at 4°C overnight. .. For detection of luciferase, signal amplification with the TSA fluorescein system (Perkin-Elmer) was performed according to the manufacturer’s instructions using a biotin-conjugated donkey anti-rabbit antibody (Jackson ImmunoResearch).

    Article Title: Type I interferon, induced by adenovirus or adenoviral vector infection, regulates the cytokine response to LPS in a macrophage type-specific manner.
    Article Snippet: .. Frozen spleens were sectioned into 8 μm-thick sections using a Leica cryostat, fixed with acetone at 0°C for 5 min and rehydrated with PBS for 30 min. To block endogenous peroxidase sections were treated with Peroxo-Block (Invitrogen) for 1 min, permeabilized in PBS containing 5% normal donkey serum and 0.1% Triton X-100 for 20 min., and then incubated with rabbit antiluciferase antibody (Fitzgerald, 70C-CR2020RAP) in PBS containing 3% normal donkey serum at 4°C overnight. .. For detection of luciferase, signal amplification with the TSA fluorescein system (Perkin-Elmer) was performed according to the manufacturer's instructions using a biotin-conjugated donkey anti-rabbit antibody (Jackson ImmunoResearch).

    Negative Control:

    Article Title: Next Generation Chemiluminescent Probes for Antimalarial Drug Discovery
    Article Snippet: Luminescence stability was quantified using a theoretical parasite per well range of 20 000–1, 3-fold diluted, and lysed in H 2 O (20 μL per well of a 384-well plate Greiner Bio-One, cat. #781086). .. 20 000 F12 wt parasites were used as negative control. β-gal SENSOR probe (Biosynth AG, cat. #A-8169_P00) was dissolved in PBS containing 1 mM MgCl 2 to a concentration of 20 μM, incubated at RT for 30–60 min and subsequently added to each well to a final concentration of 10 μM. .. Chemiluminescence was measured every 15 min (0–180 min) using PerkinElmer’s IVIS Spectrum In Vivo Imaging System Lumina LT Series III at RT.

    Concentration Assay:

    Article Title: Next Generation Chemiluminescent Probes for Antimalarial Drug Discovery
    Article Snippet: Luminescence stability was quantified using a theoretical parasite per well range of 20 000–1, 3-fold diluted, and lysed in H 2 O (20 μL per well of a 384-well plate Greiner Bio-One, cat. #781086). .. 20 000 F12 wt parasites were used as negative control. β-gal SENSOR probe (Biosynth AG, cat. #A-8169_P00) was dissolved in PBS containing 1 mM MgCl 2 to a concentration of 20 μM, incubated at RT for 30–60 min and subsequently added to each well to a final concentration of 10 μM. .. Chemiluminescence was measured every 15 min (0–180 min) using PerkinElmer’s IVIS Spectrum In Vivo Imaging System Lumina LT Series III at RT.

    Migration:

    Article Title: The modulatory effects of large and small extracellular vesicles from normal human urine on calcium oxalate crystallization, growth, aggregation, adhesion on renal cells, and invasion through extracellular matrix: An in vitro study.
    Article Snippet: .. Then, 100 μl of 0.15 pM urokinase plasminogen activator (Fitzgerald Industries International) in PBS was added, and the crystal complexes were laid on top of the matrix gel inside the ECM migration chamber. ..



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