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Alignment of parborlysin-like sequences found in the transcriptomes. N-terminal sequence boundaries were predicted using SignalP-5.0. Sequence names: REF; reference sequence, SP; SignalP-5.0-processed, NO_SP; no signal sequence found. Sequence identifiers ending in _cXXX were found in the transcriptome sequenced in this study, and identifiers ending in _DNXXX were found in SRX731467. Default Clustal colour code is used.

Journal: Toxins

Article Title: Peptide Toxins from Antarctica: The Nemertean Predator and Scavenger Parborlasia corrugatus (McIntosh, 1876)

doi: 10.3390/toxins16050209

Figure Lengend Snippet: Alignment of parborlysin-like sequences found in the transcriptomes. N-terminal sequence boundaries were predicted using SignalP-5.0. Sequence names: REF; reference sequence, SP; SignalP-5.0-processed, NO_SP; no signal sequence found. Sequence identifiers ending in _cXXX were found in the transcriptome sequenced in this study, and identifiers ending in _DNXXX were found in SRX731467. Default Clustal colour code is used.

Article Snippet: The Illumina 2500-sequenced and Trinity-assembled transcriptome produced in this study resulted in 67,148 contigs with a combined contig length of 43,298,283 bases.

Techniques: Sequencing

ClustalW alignment of conoprec-processed beta/neurotoxin-B and 8750 Da sequences found in the P. corrugatus transcriptomes. ( A ) Beta/neurotoxin B hit alignment. REF; neurotoxins B II and IV on top; nemertide beta 1 at the bottom. Grey bar marks loop 4; numerals mark Cys residues. ( B ) ClustalW alignment of conoprec-predicted mature 8750Da BLASTp hits. DN6723 does not contain a signal sequence and seems to be truncated after position 82.

Journal: Toxins

Article Title: Peptide Toxins from Antarctica: The Nemertean Predator and Scavenger Parborlasia corrugatus (McIntosh, 1876)

doi: 10.3390/toxins16050209

Figure Lengend Snippet: ClustalW alignment of conoprec-processed beta/neurotoxin-B and 8750 Da sequences found in the P. corrugatus transcriptomes. ( A ) Beta/neurotoxin B hit alignment. REF; neurotoxins B II and IV on top; nemertide beta 1 at the bottom. Grey bar marks loop 4; numerals mark Cys residues. ( B ) ClustalW alignment of conoprec-predicted mature 8750Da BLASTp hits. DN6723 does not contain a signal sequence and seems to be truncated after position 82.

Article Snippet: The Illumina 2500-sequenced and Trinity-assembled transcriptome produced in this study resulted in 67,148 contigs with a combined contig length of 43,298,283 bases.

Techniques: Sequencing

Transcriptome mining for alpha nemertides and the 3624 Da peptide. ( A ) Alignment of conoprec-predicted mature hits with similarity to alpha nemertides. Alpha 1 on top for reference. Cystine and loop numbering are marked. ( B ) Conoprec-predicted mature toxin hits with similarity to 3624 Da peptide. Note that 3266Da_c10532 is found in both the alpha and 3624 surveys. ( C ) Precursor sequences with conoprec-identified regions for 3694, 3716, and 3266 Da peptides. Alpha 1 precursor for reference. PC: post-sequence cleavage site. * Specific post-sequence cleavage sites for 3266 and alpha 1, respectively. ** Pre-sequence cleavage site. Deafault Clustal color code is used.

Journal: Toxins

Article Title: Peptide Toxins from Antarctica: The Nemertean Predator and Scavenger Parborlasia corrugatus (McIntosh, 1876)

doi: 10.3390/toxins16050209

Figure Lengend Snippet: Transcriptome mining for alpha nemertides and the 3624 Da peptide. ( A ) Alignment of conoprec-predicted mature hits with similarity to alpha nemertides. Alpha 1 on top for reference. Cystine and loop numbering are marked. ( B ) Conoprec-predicted mature toxin hits with similarity to 3624 Da peptide. Note that 3266Da_c10532 is found in both the alpha and 3624 surveys. ( C ) Precursor sequences with conoprec-identified regions for 3694, 3716, and 3266 Da peptides. Alpha 1 precursor for reference. PC: post-sequence cleavage site. * Specific post-sequence cleavage sites for 3266 and alpha 1, respectively. ** Pre-sequence cleavage site. Deafault Clustal color code is used.

Article Snippet: The Illumina 2500-sequenced and Trinity-assembled transcriptome produced in this study resulted in 67,148 contigs with a combined contig length of 43,298,283 bases.

Techniques: Sequencing