Lysis:Article Title: The beauty of being (label)-free: sample preparation methods for SWATH-MS and next-generation targeted proteomics
Article Snippet: .. Lysis buffer: SDT buffer (4% SDS, 100 mM Tris/HCl pH 7.6, 0.1 M Dithiothreitol (DTT, Melford Cat No. MB1015) ABC: 0.05 M Ammonium bicarbonate (Fluka Cat. No. 40867-50G-F) in water DTT: 0.09 M Dithiothreitol (Melford Cat No. MB1015) IAA: 0.1 M Iodoacetamide (Sigma Cat. No. I1149-5G) in ABC Sequencing Grade Modified Trypsin: Stock 0.2 μg/μL (Promega Cat. No. V5111) UPLC/MS grade water FA: Formic acid ACN: Acetonitrile 30% Acrylamide/0.8% Bis-acrylamide (Protogel, Geneflow Limited Cat. No. EC-890) 10% Ammonium persulfate (APS, Sigma Cat. No. A3678-25G) 10% Sodium dodecyl sulfate (SDS, Melford Cat. No. S1030) 1.5 M Tris/HCl pH 8.8 0.5 M Tris/HCl pH 6.8 BCA Protein assay kit (Pierce Cat. No. 23225) .. Protein Gel Casting Stand (BioRad) Acid-washed glass beads (Sigma G8772-500G) Protein LoBind tubes (Eppendorf Cat. No. 0030 108.094) Fast Prep-24 instrument (MP Biomedicals) Refrigerated bench top centrifuge Spectrophotometer Ultrasonic tank (Langford Electronics Limited) Wet chamber Vacuum concentrator centrifuge
Sequencing:Article Title: The beauty of being (label)-free: sample preparation methods for SWATH-MS and next-generation targeted proteomics
Article Snippet: .. Lysis buffer: SDT buffer (4% SDS, 100 mM Tris/HCl pH 7.6, 0.1 M Dithiothreitol (DTT, Melford Cat No. MB1015) ABC: 0.05 M Ammonium bicarbonate (Fluka Cat. No. 40867-50G-F) in water DTT: 0.09 M Dithiothreitol (Melford Cat No. MB1015) IAA: 0.1 M Iodoacetamide (Sigma Cat. No. I1149-5G) in ABC Sequencing Grade Modified Trypsin: Stock 0.2 μg/μL (Promega Cat. No. V5111) UPLC/MS grade water FA: Formic acid ACN: Acetonitrile 30% Acrylamide/0.8% Bis-acrylamide (Protogel, Geneflow Limited Cat. No. EC-890) 10% Ammonium persulfate (APS, Sigma Cat. No. A3678-25G) 10% Sodium dodecyl sulfate (SDS, Melford Cat. No. S1030) 1.5 M Tris/HCl pH 8.8 0.5 M Tris/HCl pH 6.8 BCA Protein assay kit (Pierce Cat. No. 23225) .. Protein Gel Casting Stand (BioRad) Acid-washed glass beads (Sigma G8772-500G) Protein LoBind tubes (Eppendorf Cat. No. 0030 108.094) Fast Prep-24 instrument (MP Biomedicals) Refrigerated bench top centrifuge Spectrophotometer Ultrasonic tank (Langford Electronics Limited) Wet chamber Vacuum concentrator centrifuge
Modification:Article Title: The beauty of being (label)-free: sample preparation methods for SWATH-MS and next-generation targeted proteomics
Article Snippet: .. Lysis buffer: SDT buffer (4% SDS, 100 mM Tris/HCl pH 7.6, 0.1 M Dithiothreitol (DTT, Melford Cat No. MB1015) ABC: 0.05 M Ammonium bicarbonate (Fluka Cat. No. 40867-50G-F) in water DTT: 0.09 M Dithiothreitol (Melford Cat No. MB1015) IAA: 0.1 M Iodoacetamide (Sigma Cat. No. I1149-5G) in ABC Sequencing Grade Modified Trypsin: Stock 0.2 μg/μL (Promega Cat. No. V5111) UPLC/MS grade water FA: Formic acid ACN: Acetonitrile 30% Acrylamide/0.8% Bis-acrylamide (Protogel, Geneflow Limited Cat. No. EC-890) 10% Ammonium persulfate (APS, Sigma Cat. No. A3678-25G) 10% Sodium dodecyl sulfate (SDS, Melford Cat. No. S1030) 1.5 M Tris/HCl pH 8.8 0.5 M Tris/HCl pH 6.8 BCA Protein assay kit (Pierce Cat. No. 23225) .. Protein Gel Casting Stand (BioRad) Acid-washed glass beads (Sigma G8772-500G) Protein LoBind tubes (Eppendorf Cat. No. 0030 108.094) Fast Prep-24 instrument (MP Biomedicals) Refrigerated bench top centrifuge Spectrophotometer Ultrasonic tank (Langford Electronics Limited) Wet chamber Vacuum concentrator centrifuge
Bicinchoninic Acid Protein Assay:Article Title: The beauty of being (label)-free: sample preparation methods for SWATH-MS and next-generation targeted proteomics
Article Snippet: .. Lysis buffer: SDT buffer (4% SDS, 100 mM Tris/HCl pH 7.6, 0.1 M Dithiothreitol (DTT, Melford Cat No. MB1015) ABC: 0.05 M Ammonium bicarbonate (Fluka Cat. No. 40867-50G-F) in water DTT: 0.09 M Dithiothreitol (Melford Cat No. MB1015) IAA: 0.1 M Iodoacetamide (Sigma Cat. No. I1149-5G) in ABC Sequencing Grade Modified Trypsin: Stock 0.2 μg/μL (Promega Cat. No. V5111) UPLC/MS grade water FA: Formic acid ACN: Acetonitrile 30% Acrylamide/0.8% Bis-acrylamide (Protogel, Geneflow Limited Cat. No. EC-890) 10% Ammonium persulfate (APS, Sigma Cat. No. A3678-25G) 10% Sodium dodecyl sulfate (SDS, Melford Cat. No. S1030) 1.5 M Tris/HCl pH 8.8 0.5 M Tris/HCl pH 6.8 BCA Protein assay kit (Pierce Cat. No. 23225) .. Protein Gel Casting Stand (BioRad) Acid-washed glass beads (Sigma G8772-500G) Protein LoBind tubes (Eppendorf Cat. No. 0030 108.094) Fast Prep-24 instrument (MP Biomedicals) Refrigerated bench top centrifuge Spectrophotometer Ultrasonic tank (Langford Electronics Limited) Wet chamber Vacuum concentrator centrifuge
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