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loading buffer  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology loading buffer
    Loading Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sample+loading+buffer/RNA+Sample+Loading+Buffer/pm28525371-146-5-19
    Average 94 stars, based on 1 article reviews
    loading buffer - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: SARS-CoV-2 Infection Reactivates HIV-1 Replication From Latency in U1 Cells.
    Article Snippet: The global impact of COVID‐19, caused by SARS‐CoV‐2, has infected millions, including those with HIV‐1.. However, it is unclear if SARS‐CoV‐2 affects HIV‐1 reactivation from latency.. Here, we used the U1 cell line to explore how SARS‐CoV‐2 infection affects HIV‐1 reactivation from latency, employing real‐time PCR assays and Western blot analysis.

    Incubation:

    Article Title: Cryptotanshinone-Induced p53-Dependent Sensitization of Colon Cancer Cells to Apoptotic Drive by Regulation of Calpain and Calcium Homeostasis
    Article Snippet: Over-expression of calpains in tumor tissues can be associated with cancer progression.. Thus, inhibition of calpain activity using specific inhibitors has become a novel approach to control tumor growth.. In this study, the anticancer potential of cryptotanshinone in combination with calpain inhibitor had been investigated in colon cancer cells and tumor xenograft.

    Article Title: The neuronal proteins CIPP, Cypin and IRSp53 form a tripartite complex mediated by PDZ and SH3 domains
    Article Snippet: Here we report the dissection of a tripartite complex formed by CIPP (channel-interacting PDZ protein), IRSp53 (insulin receptor tyrosine kinase substrate protein) and Cypin (cytosolic PSD-95 interactor) in cultured cells.. The three proteins are expressed in similar neuronal districts, where CIPP binds to different membrane channels and receptors, IRSp53 regulates the morphogenesis of actin-rich dendritic spines, and Cypin promotes dendrite branching and patterning by binding to tubulin heterodimers.. We observed that the interaction among the three proteins is mediated by small binding domains: CIPP works as a bridge, linking the carboxy-termini of IRSp53 and Cypin with its PDZ domains; IRSp53 connects Cypin, through an unusual SH3-mediated association, which can be impaired by substituting two crucial positively charged residues of Cypin.

    Centrifugation:

    Article Title: Cryptotanshinone-Induced p53-Dependent Sensitization of Colon Cancer Cells to Apoptotic Drive by Regulation of Calpain and Calcium Homeostasis
    Article Snippet: Over-expression of calpains in tumor tissues can be associated with cancer progression.. Thus, inhibition of calpain activity using specific inhibitors has become a novel approach to control tumor growth.. In this study, the anticancer potential of cryptotanshinone in combination with calpain inhibitor had been investigated in colon cancer cells and tumor xenograft.

    Transfection:

    Article Title: Cryptotanshinone-Induced p53-Dependent Sensitization of Colon Cancer Cells to Apoptotic Drive by Regulation of Calpain and Calcium Homeostasis
    Article Snippet: Over-expression of calpains in tumor tissues can be associated with cancer progression.. Thus, inhibition of calpain activity using specific inhibitors has become a novel approach to control tumor growth.. In this study, the anticancer potential of cryptotanshinone in combination with calpain inhibitor had been investigated in colon cancer cells and tumor xenograft.

    SDS Page:

    Article Title: Crohn's Disease-associated variant in laccase domain containing 1 (LACC1) modulates T cell gene expression, metabolism and T cell function.
    Article Snippet: Protein concentration of the supernatant wasmeasured using the BCA Protein Assay (Thermo Scientific). .. Proteins were denatured by boiling for 10min in SDS sample loading buffer and then loaded on 12% SDS-PAGE gel for electrophoresis, followed by transfer to a polyvinylidene difluoride transfer membrane (Santa Cruz). ..

    Article Title: Crohn’s Disease-associated variant in laccase domain containing 1 ( LACC1 ) modulates T cell gene expression, metabolism and T cell function
    Article Snippet: Protein concentration of the supernatant was measured using the BCA Protein Assay (Thermo Scientific). .. Proteins were denatured by boiling for 10 min in SDS sample loading buffer and then loaded on 12% SDS-PAGE gel for electrophoresis, followed by transfer to a polyvinylidene difluoride transfer membrane (Santa Cruz). ..

    Electrophoresis:

    Article Title: Crohn's Disease-associated variant in laccase domain containing 1 (LACC1) modulates T cell gene expression, metabolism and T cell function.
    Article Snippet: Protein concentration of the supernatant wasmeasured using the BCA Protein Assay (Thermo Scientific). .. Proteins were denatured by boiling for 10min in SDS sample loading buffer and then loaded on 12% SDS-PAGE gel for electrophoresis, followed by transfer to a polyvinylidene difluoride transfer membrane (Santa Cruz). ..

    Article Title: Crohn’s Disease-associated variant in laccase domain containing 1 ( LACC1 ) modulates T cell gene expression, metabolism and T cell function
    Article Snippet: Protein concentration of the supernatant was measured using the BCA Protein Assay (Thermo Scientific). .. Proteins were denatured by boiling for 10 min in SDS sample loading buffer and then loaded on 12% SDS-PAGE gel for electrophoresis, followed by transfer to a polyvinylidene difluoride transfer membrane (Santa Cruz). ..

    Membrane:

    Article Title: Crohn's Disease-associated variant in laccase domain containing 1 (LACC1) modulates T cell gene expression, metabolism and T cell function.
    Article Snippet: Protein concentration of the supernatant wasmeasured using the BCA Protein Assay (Thermo Scientific). .. Proteins were denatured by boiling for 10min in SDS sample loading buffer and then loaded on 12% SDS-PAGE gel for electrophoresis, followed by transfer to a polyvinylidene difluoride transfer membrane (Santa Cruz). ..

    Article Title: Crohn’s Disease-associated variant in laccase domain containing 1 ( LACC1 ) modulates T cell gene expression, metabolism and T cell function
    Article Snippet: Protein concentration of the supernatant was measured using the BCA Protein Assay (Thermo Scientific). .. Proteins were denatured by boiling for 10 min in SDS sample loading buffer and then loaded on 12% SDS-PAGE gel for electrophoresis, followed by transfer to a polyvinylidene difluoride transfer membrane (Santa Cruz). ..

    other:

    Article Title: Arsenic trioxide and angiotensin II have inhibitory effects on HERG protein expression: Evidence for the role of PML SUMOylation.
    Article Snippet: Primary antibodies against PML (Santa Cruz Biotechnology, Santa Cruz, CA, U.S.) and Pin1 (Santa Cruz Biotechnology, Santa Cruz, CA, U.S.) were incubated with the protein samples at 4°C overnight on a shaker.

    Article Title: The MORN domain of Junctophilin2 regulates functional interactions with small-conductance Ca 2+ -activated potassium channel subtype2 (SK2).
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Numbers: 81270248, 81570311 Abstract Small-conductance Ca-activated K channel subtype2 (SK2) are stable macromolecular complexes that regulate myocardial excitability and Ca homeostasis.. Junctophilin-2 (JP2) is a membrane-binding protein, which provides functional crosstalk by physically linking with the cell-surface and intracellular ion channels.. We previously demonstrated that the MORN domain of JP2 interacts with SK2 channels.

    Lysis:

    Article Title: The neuronal proteins CIPP, Cypin and IRSp53 form a tripartite complex mediated by PDZ and SH3 domains
    Article Snippet: Here we report the dissection of a tripartite complex formed by CIPP (channel-interacting PDZ protein), IRSp53 (insulin receptor tyrosine kinase substrate protein) and Cypin (cytosolic PSD-95 interactor) in cultured cells.. The three proteins are expressed in similar neuronal districts, where CIPP binds to different membrane channels and receptors, IRSp53 regulates the morphogenesis of actin-rich dendritic spines, and Cypin promotes dendrite branching and patterning by binding to tubulin heterodimers.. We observed that the interaction among the three proteins is mediated by small binding domains: CIPP works as a bridge, linking the carboxy-termini of IRSp53 and Cypin with its PDZ domains; IRSp53 connects Cypin, through an unusual SH3-mediated association, which can be impaired by substituting two crucial positively charged residues of Cypin.

    Western Blot:

    Article Title: The neuronal proteins CIPP, Cypin and IRSp53 form a tripartite complex mediated by PDZ and SH3 domains
    Article Snippet: Here we report the dissection of a tripartite complex formed by CIPP (channel-interacting PDZ protein), IRSp53 (insulin receptor tyrosine kinase substrate protein) and Cypin (cytosolic PSD-95 interactor) in cultured cells.. The three proteins are expressed in similar neuronal districts, where CIPP binds to different membrane channels and receptors, IRSp53 regulates the morphogenesis of actin-rich dendritic spines, and Cypin promotes dendrite branching and patterning by binding to tubulin heterodimers.. We observed that the interaction among the three proteins is mediated by small binding domains: CIPP works as a bridge, linking the carboxy-termini of IRSp53 and Cypin with its PDZ domains; IRSp53 connects Cypin, through an unusual SH3-mediated association, which can be impaired by substituting two crucial positively charged residues of Cypin.



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