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macsima running buffer (Miltenyi Biotec)

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Miltenyi Biotec macsima running buffer
Macsima Running Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/running+buffer/MACSima+Running+Buffer/pm40513575-446-7-10
Average 95 stars, based on 32 article reviews

macsima running buffer - by Bioz Stars, 2026-10

95/100 stars

Images

Blocking Assay:

Article Title: Immune checkpoint blockade activates macrophages and remodels the lymph node microenvironment in melanoma metastasis
Article Snippet: .. Tissues were fixed in cold acetone for 20min, then washed withMACSima Running Buffer (Miltenyi Biotec, # 130-121-565), which was also used for blocking for 30min at room temperature before probing with primary antibodies diluted inMACSima Running Buffer overnight at 4 °C. .. This was followed by washing of the slides with MACSima Running Buffer, prestaining of nuclei using DAPI Staining Solution (Miltenyi Biotec, component of MACSima Stain Support Kit, # 130-127-574) to incubate the slides with the diluted solution for 10min in the dark at room temperature, and a final washing step.

Staining:

Article Title: Intravitreal AAV vector delivery induces integrin-dependent ocular inflammation, complement activation, and antiviral and DNA damage responses
Article Snippet: Fixed ocular globes were sectioned at 7μM, and slides were loaded onto the MACSima Imaging System four-well cassette (Miltenyi Biotech). .. Slides were subsequently stained with DAPI for 10 min and washed thrice with MACSima running buffer (Miltenyi Biotech). ..

Incubation:

Article Title: Interferon-elicited lipoprotein metabolism in lung fibroblasts facilitates premetastatic niche formation.
Article Snippet: Afterward, 20 μL of Fibroblast MicroBeads was added, followed by an incubation for 30 minutes at room temperature. .. After the incubation, the cells were washed with 1–2 mL of running buffer via centrifugation (300 g, 10 minutes) and resuspended in 500 μL of running buffer (Miltenyi Biotec, Cat# 130-121-565). .. LS columns (Miltenyi Biotec, Cat#130-042-401) were prerinsed with 3 mL of running buffer before the cell suspensions were loaded.

Centrifugation:

Imaging:

Article Title: Single Cell Analysis Reveals the Presence of Novel Intermediate Cells in Both Mice and Patients With Severe MASLD
Article Snippet: .. Liver frozen embedded tissues, 4 μm thick, were mounted on SuperFrost Plus slides and fixed with a 4% paraformaldehyde solution for 10 min at RT, rinsed three times with MACSima Running Buffer (130‐121‐565, Miltenyi Biotec, Bergisch Gladbach, Germany), and placed onto MACSwell Four Imaging Frames (130‐124‐676, Miltenyi Biotec). .. Sections were then pre‐treated with DAPI at a 1:5 ratio (130‐127‐574, Miltenyi Biotec) in the Running Buffer for 20 min, washed three times with Running Buffer, and finalized with a sample volume of 250 μL.