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Promega reverse transcriptase reaction mixture
Reverse Transcriptase Reaction Mixture, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcriptase+mixture/reverse+transcriptase+reaction+mixture/pmc03577834-190-34-38
Average 90 stars, based on 1 article reviews
reverse transcriptase reaction mixture - by Bioz Stars, 2026-10
90/100 stars

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Synthesized:

Article Title: Arctigenin ameliorates depression-like behaviors in Toxoplasma gondii-infected intermediate hosts via the TLR4/NF-κB and TNFR1/NF-κB signaling pathways.
Article Snippet: Toxoplasma gondii (T. gondii) is a known neurotropic protozoan that remains in the central nervous system and induces neuropsychiatric diseases in intermediate hosts.. Arctigenin (AG) is one of the major bioactive lignans of the fruit Arctium lappa L. and has a broad spectrum of pharmacological activities such as neuroprotective, antiinflammatory and anti-T. gondii effects.. However, the effect of AG against depressive behaviors observed in T. gondii-infected hosts has not yet been clarified.

Article Title: Synergistic growth inhibition based on small-molecule p53 activation as treatment for intraocular melanoma.
Article Snippet: RNA was isolated using the SV Total RNA isolation kit (Promega, Madison, WI, USA). .. Complementary DNA was synthesized using 2.0mg RNA in a total volume of 30ml reverse transcriptase reaction mixture (Promega). .. Samples were analyzed in triplicate using SYBR-Green mix (Roche Biochemicals, Indianapolis, IN, USA) in a 7900ht Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA).

Article Title: Targeting MDMX and PKCδ to improve current uveal melanoma therapeutic strategies
Article Snippet: .. RNA was isolated from cells using the SV total RNA isolation kit (Promega, Fitchburg, WI, USA), from which complementary DNA (cDNA) was synthesized using the reverse transcriptase reaction mixture as indicated by Promega. .. Quantitative PCR (qPCR) was performed using SYBR green mix (Roche, Basel, Switzerland) in a C1000 touch Thermal Cycler (Bio-Rad Laboratories, Hercules, CA, USA).

Article Title: MDMX Regulates Transcriptional Activity of p53 and FOXO Proteins to Stimulate Proliferation of Melanoma Cells
Article Snippet: .. RNA was isolated using the SV total RNA isolation kit (Promega), after which cDNA was synthesized using the reverse transcriptase reaction mixture as indicated by Promega. qPCR was performed using SYBR green mix (Roche Diagnostics, Indianapolis, IN, USA) in a C1000 touch Thermal Cycler (Bio-Rad laboratories, Hercules, CA, USA). ..

Article Title: Bmi1 Enhances Tumorigenicity and Cancer Stem Cell Function in Pancreatic Adenocarcinoma
Article Snippet: .. Total RNA was isolated from sorted primary human pancreatic cancer cells as described previously . cDNA was first synthesized using equivalent amounts of total RNA (0.5–1 μg) with random primers in a 20 μL reverse transcriptase reaction mixture (Promega, Madison, WI). .. Real-time quantitative RT-PCR (Taqman) primers for Bmi1 were designed and purchased from Applied Biosystems (Foster City, CA) as Assay-on-Demand Gene Expression Products.

Article Title: Differential Expression of DNA Repair Genes in Prognostically-Favorable versus Unfavorable Uveal Melanoma
Article Snippet: Gene-expression profiling at the transcriptional level was carried out on RNA of 64 UMs using 35,244 probes from the Illumina HT-12v4 chip (Illumina, San Diego, CA, USA). .. RNA for real-time PCR analysis in cell lines was isolated using the SV total RNA isolation kit (Promega, Madison, WI, USA), then cDNA was synthesized using the reverse transcriptase reaction mixture, as indicated by Promega. qPCR was performed using SYBR green mix (Roche Diagnostics, IN, USA) in a C1000 touch Thermal Cycler (Bio-Rad laboratories, Hercules, CA, USA). ..

Reverse Transcription:

Article Title: Arctigenin ameliorates depression-like behaviors in Toxoplasma gondii-infected intermediate hosts via the TLR4/NF-κB and TNFR1/NF-κB signaling pathways.
Article Snippet: Toxoplasma gondii (T. gondii) is a known neurotropic protozoan that remains in the central nervous system and induces neuropsychiatric diseases in intermediate hosts.. Arctigenin (AG) is one of the major bioactive lignans of the fruit Arctium lappa L. and has a broad spectrum of pharmacological activities such as neuroprotective, antiinflammatory and anti-T. gondii effects.. However, the effect of AG against depressive behaviors observed in T. gondii-infected hosts has not yet been clarified.

Article Title: Synergistic growth inhibition based on small-molecule p53 activation as treatment for intraocular melanoma.
Article Snippet: RNA was isolated using the SV Total RNA isolation kit (Promega, Madison, WI, USA). .. Complementary DNA was synthesized using 2.0mg RNA in a total volume of 30ml reverse transcriptase reaction mixture (Promega). .. Samples were analyzed in triplicate using SYBR-Green mix (Roche Biochemicals, Indianapolis, IN, USA) in a 7900ht Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA).

Article Title: Targeting MDMX and PKCδ to improve current uveal melanoma therapeutic strategies
Article Snippet: .. RNA was isolated from cells using the SV total RNA isolation kit (Promega, Fitchburg, WI, USA), from which complementary DNA (cDNA) was synthesized using the reverse transcriptase reaction mixture as indicated by Promega. .. Quantitative PCR (qPCR) was performed using SYBR green mix (Roche, Basel, Switzerland) in a C1000 touch Thermal Cycler (Bio-Rad Laboratories, Hercules, CA, USA).

Article Title: MDMX Regulates Transcriptional Activity of p53 and FOXO Proteins to Stimulate Proliferation of Melanoma Cells
Article Snippet: .. RNA was isolated using the SV total RNA isolation kit (Promega), after which cDNA was synthesized using the reverse transcriptase reaction mixture as indicated by Promega. qPCR was performed using SYBR green mix (Roche Diagnostics, Indianapolis, IN, USA) in a C1000 touch Thermal Cycler (Bio-Rad laboratories, Hercules, CA, USA). ..

Article Title: Bmi1 Enhances Tumorigenicity and Cancer Stem Cell Function in Pancreatic Adenocarcinoma
Article Snippet: .. Total RNA was isolated from sorted primary human pancreatic cancer cells as described previously . cDNA was first synthesized using equivalent amounts of total RNA (0.5–1 μg) with random primers in a 20 μL reverse transcriptase reaction mixture (Promega, Madison, WI). .. Real-time quantitative RT-PCR (Taqman) primers for Bmi1 were designed and purchased from Applied Biosystems (Foster City, CA) as Assay-on-Demand Gene Expression Products.

Article Title: Differential Expression of DNA Repair Genes in Prognostically-Favorable versus Unfavorable Uveal Melanoma
Article Snippet: Gene-expression profiling at the transcriptional level was carried out on RNA of 64 UMs using 35,244 probes from the Illumina HT-12v4 chip (Illumina, San Diego, CA, USA). .. RNA for real-time PCR analysis in cell lines was isolated using the SV total RNA isolation kit (Promega, Madison, WI, USA), then cDNA was synthesized using the reverse transcriptase reaction mixture, as indicated by Promega. qPCR was performed using SYBR green mix (Roche Diagnostics, IN, USA) in a C1000 touch Thermal Cycler (Bio-Rad laboratories, Hercules, CA, USA). ..

other:

Article Title: Compositions and methods for treating and diagnosing pancreatic cancer
Article Snippet: For real-time RT-PCR analysis, cDNA was first synthesized using equivalent amounts of total RNA (0.5-1 μg) with random primers in a 20 μl reverse-transcriptase reaction mixture (Promega).

Article Title: Inhibitory effects of metformin at low concentration on epithelial–mesenchymal transition of CD44 + CD117 + ovarian cancer stem cells
Article Snippet: cDNA was first synthesized using equivalent amounts of total RNA (0.5-1 μg) with random primers in a 20 μl reverse-transcriptase reaction mixture (Promega).

Isolation:

Article Title: Targeting MDMX and PKCδ to improve current uveal melanoma therapeutic strategies
Article Snippet: .. RNA was isolated from cells using the SV total RNA isolation kit (Promega, Fitchburg, WI, USA), from which complementary DNA (cDNA) was synthesized using the reverse transcriptase reaction mixture as indicated by Promega. .. Quantitative PCR (qPCR) was performed using SYBR green mix (Roche, Basel, Switzerland) in a C1000 touch Thermal Cycler (Bio-Rad Laboratories, Hercules, CA, USA).

Article Title: MDMX Regulates Transcriptional Activity of p53 and FOXO Proteins to Stimulate Proliferation of Melanoma Cells
Article Snippet: .. RNA was isolated using the SV total RNA isolation kit (Promega), after which cDNA was synthesized using the reverse transcriptase reaction mixture as indicated by Promega. qPCR was performed using SYBR green mix (Roche Diagnostics, Indianapolis, IN, USA) in a C1000 touch Thermal Cycler (Bio-Rad laboratories, Hercules, CA, USA). ..

Article Title: Bmi1 Enhances Tumorigenicity and Cancer Stem Cell Function in Pancreatic Adenocarcinoma
Article Snippet: .. Total RNA was isolated from sorted primary human pancreatic cancer cells as described previously . cDNA was first synthesized using equivalent amounts of total RNA (0.5–1 μg) with random primers in a 20 μL reverse transcriptase reaction mixture (Promega, Madison, WI). .. Real-time quantitative RT-PCR (Taqman) primers for Bmi1 were designed and purchased from Applied Biosystems (Foster City, CA) as Assay-on-Demand Gene Expression Products.

Article Title: Differential Expression of DNA Repair Genes in Prognostically-Favorable versus Unfavorable Uveal Melanoma
Article Snippet: Gene-expression profiling at the transcriptional level was carried out on RNA of 64 UMs using 35,244 probes from the Illumina HT-12v4 chip (Illumina, San Diego, CA, USA). .. RNA for real-time PCR analysis in cell lines was isolated using the SV total RNA isolation kit (Promega, Madison, WI, USA), then cDNA was synthesized using the reverse transcriptase reaction mixture, as indicated by Promega. qPCR was performed using SYBR green mix (Roche Diagnostics, IN, USA) in a C1000 touch Thermal Cycler (Bio-Rad laboratories, Hercules, CA, USA). ..

Real-time Polymerase Chain Reaction:

Article Title: MDMX Regulates Transcriptional Activity of p53 and FOXO Proteins to Stimulate Proliferation of Melanoma Cells
Article Snippet: .. RNA was isolated using the SV total RNA isolation kit (Promega), after which cDNA was synthesized using the reverse transcriptase reaction mixture as indicated by Promega. qPCR was performed using SYBR green mix (Roche Diagnostics, Indianapolis, IN, USA) in a C1000 touch Thermal Cycler (Bio-Rad laboratories, Hercules, CA, USA). ..

Article Title: Differential Expression of DNA Repair Genes in Prognostically-Favorable versus Unfavorable Uveal Melanoma
Article Snippet: Gene-expression profiling at the transcriptional level was carried out on RNA of 64 UMs using 35,244 probes from the Illumina HT-12v4 chip (Illumina, San Diego, CA, USA). .. RNA for real-time PCR analysis in cell lines was isolated using the SV total RNA isolation kit (Promega, Madison, WI, USA), then cDNA was synthesized using the reverse transcriptase reaction mixture, as indicated by Promega. qPCR was performed using SYBR green mix (Roche Diagnostics, IN, USA) in a C1000 touch Thermal Cycler (Bio-Rad laboratories, Hercules, CA, USA). ..

SYBR Green Assay:

Article Title: MDMX Regulates Transcriptional Activity of p53 and FOXO Proteins to Stimulate Proliferation of Melanoma Cells
Article Snippet: .. RNA was isolated using the SV total RNA isolation kit (Promega), after which cDNA was synthesized using the reverse transcriptase reaction mixture as indicated by Promega. qPCR was performed using SYBR green mix (Roche Diagnostics, Indianapolis, IN, USA) in a C1000 touch Thermal Cycler (Bio-Rad laboratories, Hercules, CA, USA). ..

Article Title: Differential Expression of DNA Repair Genes in Prognostically-Favorable versus Unfavorable Uveal Melanoma
Article Snippet: Gene-expression profiling at the transcriptional level was carried out on RNA of 64 UMs using 35,244 probes from the Illumina HT-12v4 chip (Illumina, San Diego, CA, USA). .. RNA for real-time PCR analysis in cell lines was isolated using the SV total RNA isolation kit (Promega, Madison, WI, USA), then cDNA was synthesized using the reverse transcriptase reaction mixture, as indicated by Promega. qPCR was performed using SYBR green mix (Roche Diagnostics, IN, USA) in a C1000 touch Thermal Cycler (Bio-Rad laboratories, Hercules, CA, USA). ..



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