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reverse transcriptase  (tiangen biotech co)


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    Structured Review

    tiangen biotech co reverse transcriptase
    Reverse Transcriptase, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase+mixture/Reverse+Transcriptase+Mix/pmc12064826-223-13-15
    Average 96 stars, based on 71 article reviews
    reverse transcriptase - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: Genomic, Probiotic, and Functional Properties of Bacteroides dorei RX2020 Isolated from Gut Microbiota.
    Article Snippet: Converting the isolated tissues RNA into complementary DNA (cDNA) using reverse transcriptase (TIANGEN, Beijing, China) and specific primers (Table S1).

    Article Title: Profiling and functional characterization of long noncoding RNAs during human tooth development
    Article Snippet: Human fetal craniofacial tissues from 17 gw were analyzed using ISH with the RNAscope Multiplex Fluorescent Reagent Kit v2 (Advanced Cell Diagnostics, CA, USA).

    Article Title: Genomic, Probiotic, and Functional Properties of Bacteroides dorei RX2020 Isolated from Gut Microbiota
    Article Snippet: Converting the isolated tissues RNA into complementary DNA (cDNA) using reverse transcriptase (TIANGEN, Beijing, China) and specific primers ( ).

    Article Title: Chloride intracellular channel 6 inhibits hepatocellular carcinoma progression by modulating immune cell balance and promoting tumor cell apoptosis
    Article Snippet: Subsequently, the RNA was transcribed into complementary DNA (cDNA) using reverse transcriptase (KR103, TIANGEN, Beijing, China), with the addition of specific primers to enhance cDNA synthesis efficiency.

    Article Title: CXCL7 enhances RANKL-induced osteoclastogenesis via the activation of ERK/NFATc1 signaling pathway in inflammatory arthritis
    Article Snippet: Total RNA was isolated using TRIzol reagent (Invitrogen) and single-stranded cDNA was synthesized from 1 μg of total RNA using reverse transcriptase (Tiangen Biotech).

    Isolation:

    Article Title: Analysis of ferroptosis-related genes in cerebral ischemic stroke via immune infiltration and single-cell RNA-sequencing
    Article Snippet: .. By applying Trizol reagent (Invitrogen, Thermo Fisher Scientific), total RNAs were isolated from brain tissue for preparation of cDNA with reverse transcriptase ((TIANGEN, China) according to corresponding instructions. .. SYBR green kit (TIANGEN, China) was then adopted for qRT-PCR based on the PCR system (BioRad, Singapore).

    Article Title: Analysis of ferroptosis-related genes in cerebral ischemic stroke via immune infiltration and single-cell RNA-sequencing.
    Article Snippet: 4′,6-diamidino-2-phenylindole (DAPI) (Abcam, ab104139) was later added for nuclear staining, whereas the fluorescence microscope (Nikon, Y-TV55, JAPAN) was adopted for acquiring images, and ImageJ was adopted for analyzing the immunofluorescence area (peri-ischemic area on ischemic hemisphere). .. The experiment was double-blinded, and diverse group members read and analyzed the images. qRT-PCR By applying Trizol reagent (Invitrogen, Thermo Fisher Scientific), total RNAs were isolated from brain tissue for preparation of cDNA with reverse transcriptase ((TIANGEN, China) according to corresponding instructions. .. SYBR green kit (TIANGEN, China) was then adopted for qRT-PCR based on the PCR system (BioRad, Singapore).

    Reverse Transcription:

    Article Title: Analysis of ferroptosis-related genes in cerebral ischemic stroke via immune infiltration and single-cell RNA-sequencing
    Article Snippet: .. By applying Trizol reagent (Invitrogen, Thermo Fisher Scientific), total RNAs were isolated from brain tissue for preparation of cDNA with reverse transcriptase ((TIANGEN, China) according to corresponding instructions. .. SYBR green kit (TIANGEN, China) was then adopted for qRT-PCR based on the PCR system (BioRad, Singapore).

    Article Title: Analysis of ferroptosis-related genes in cerebral ischemic stroke via immune infiltration and single-cell RNA-sequencing.
    Article Snippet: 4′,6-diamidino-2-phenylindole (DAPI) (Abcam, ab104139) was later added for nuclear staining, whereas the fluorescence microscope (Nikon, Y-TV55, JAPAN) was adopted for acquiring images, and ImageJ was adopted for analyzing the immunofluorescence area (peri-ischemic area on ischemic hemisphere). .. The experiment was double-blinded, and diverse group members read and analyzed the images. qRT-PCR By applying Trizol reagent (Invitrogen, Thermo Fisher Scientific), total RNAs were isolated from brain tissue for preparation of cDNA with reverse transcriptase ((TIANGEN, China) according to corresponding instructions. .. SYBR green kit (TIANGEN, China) was then adopted for qRT-PCR based on the PCR system (BioRad, Singapore).

    Quantitative RT-PCR:

    Article Title: Analysis of ferroptosis-related genes in cerebral ischemic stroke via immune infiltration and single-cell RNA-sequencing.
    Article Snippet: 4′,6-diamidino-2-phenylindole (DAPI) (Abcam, ab104139) was later added for nuclear staining, whereas the fluorescence microscope (Nikon, Y-TV55, JAPAN) was adopted for acquiring images, and ImageJ was adopted for analyzing the immunofluorescence area (peri-ischemic area on ischemic hemisphere). .. The experiment was double-blinded, and diverse group members read and analyzed the images. qRT-PCR By applying Trizol reagent (Invitrogen, Thermo Fisher Scientific), total RNAs were isolated from brain tissue for preparation of cDNA with reverse transcriptase ((TIANGEN, China) according to corresponding instructions. .. SYBR green kit (TIANGEN, China) was then adopted for qRT-PCR based on the PCR system (BioRad, Singapore).

    Control:

    Article Title: Trichoderma longibrachiatum (T6) Peptaibols Inhibiting the Monilia yunnanensis Growth and Inducing Pear Fruit Resistance in Its Infection.
    Article Snippet: .. Transcriptase–PCR (qRT–PCR) The samples for treatment and control groups were collected at 3 days after inoculation, and the samples’ total RNA was extracted using the E.Z.N.A.® Plant RNA Kit (Tiangen Biotechnology, Beijing, China). .. The quantity and quality of the total RNA were assessed using a NanoDrop spectrophotometer at absorbance wavelengths of 230 and 260 nm.



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